[Histonet] low signal for long post fixation

[Mariela Chertoff]

Dear all,

I have mouse brain tissue perfused with PFA 4% and post fixed in formol 10%
for periods between 2 month and 1 year. I cut them on vibratome (30um) and
I made inmunohistochemistry and beta galactosidase to check senescence.
My problem is that I have less signal on positive controls if postfixation
on formol was longer.

There is a way to solve this problem?

Thanks in advance for your reply

Mariela Chertoff, PhD
Laboratorio de Neuroepigenetica - QB75
 Departamento de Química Biológica
Facultad de Ciencias Exactas y Naturales - UBA
Ciudad Universitaria Pabellón II Piso 4
Ciudad Autónoma de Buenos Aires
C1428EGA - Argentina
Tel: 54 11 4576-3300/09 - Int. 221
email:marielachertoff at gmail.com
marielachertoff at qb.fcen.uba.ar
<mariela.chertoff at uab.cat>