[Histonet] Sections falling off
j.benavides at eae.csic.es
Thu Mar 23 06:56:46 CDT 2017
Just to add another advice,
Pablo, yesterday I told you that we were drying the slides overnight at
37º... not true!! (that happens for me speaking without checking with
the really knowledgeable ones, the technicians!!
We used to have that same problem and then began to dry at 60º for one
hour straight after cutting, then, after that hour in the oven straight
to staining. We don´t wash under tap water, as we have problems with
variable water pressure, so instead, 3x5 min wash.
Hope this helps
El 23/03/2017 a las 10:40, Pablo Sánchez Quinteiro via Histonet escribió:
> Thanks a lot!
> El 22/03/2017 a las 20:33, LitePath escribió:
>> Hi Pablo
>> This article just came out, I actually have not read yet, just
>> downloaded yesterday...thought I would send to your way as it might
>> be helpful...
>> Luis Chiriboga PhD
>> Director IHC
>> Experimental Pathology
>> *From:* Pablo Sánchez Quinteiro via Histonet
>> <histonet at lists.utsouthwestern.edu>
>> *To:* histonet at lists.utsouthwestern.edu
>> *Sent:* Wednesday, March 22, 2017 1:50 PM
>> *Subject:* [Histonet] Sections falling off
>> Dear Histonetters,
>> I am in trouble with my paraffin sections -samples fixed in Bouin's
>> Just after the first steps: Two xylol baths and 100% ethanol most of
>> them are falling off. Slides are silanised.
>> I do not think it was an adhesion problem. What do you think about?
>> Embedding, cuttibg?
>> Thanks a lot in advance
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