[Histonet] Sections falling off

[Pablo Sánchez Quinteiro]

Thanks for your reply Shirley.

After 24 h fixation in Bouin samples are passed into 70º ethanol.

I have tried drying the sections at RT for short or long period (30´) 
and then 37º oven overnight.

The tissue is mouse brain, sections thickness 8 microns.

Pablo



El 22/03/2017 a las 18:45, Shirley A. Powell escribió:
> Are you washing the bouins out of the tissue well before processing, that may be one source.  Also how long are you drying your sections before staining? What type of tissue are you processing?
>
> -----Original Message-----
> From: Pablo Sánchez Quinteiro via Histonet [mailto:histonet at lists.utsouthwestern.edu]
> Sent: Wednesday, March 22, 2017 1:39 PM
> To: histonet at lists.utsouthwestern.edu
> Subject: [Histonet] Sections falling off
>
> Dear Histonetters,
>
> I am in trouble with my paraffin sections -samples fixed in Bouin's liquid.
>
> Just after the first steps: Two xylol baths and 100% ethanol most of them are falling off. Slides are silanised.
>
> I do not think it was an adhesion problem. What do you think about?
> Embedding, cuttibg?
>
> Thanks a lot in advance
>
> Pablo
>
>
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