[Histonet] Sections falling off

[Manfre, Philip]

-----Original Message-----
From: Pablo Sánchez Quinteiro via Histonet [mailto:histonet at lists.utsouthwestern.edu] 
Sent: Wednesday, March 22, 2017 1:39 PM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Sections falling off

Dear Histonetters,

I am in trouble with my paraffin sections -samples fixed in Bouin's liquid.

Pablo,
	I think your sections are not completely dry, after placing them on slides.  Silanized slides can trap water between the sections and the slide, almost appearing like a bulge.  You may need to use the corner of a paper towel to "poke" the paraffin section and drain off the excess water.  Make sure the sections look dry before starting the staining procedure.  They should be transparent (translucent), not opaque (milky white).
	That's my guess

Phil.

Philip Manfre, HT (ASCP)
Associate Principal Scientist
Merck Research Laboratories
WP81-406
770 Sumneytown Pike
West Point, PA 19486

215-652-9750
215-993-0383 (fax)
philip_manfre at merck.com




Just after the first steps: Two xylol baths and 100% ethanol most of 
them are falling off. Slides are silanised.

I do not think it was an adhesion problem. What do you think about? 
Embedding, cuttibg?

Thanks a lot in advance

Pablo


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