[Histonet] Bone Processing

[MICHELLE BETH TITUNICK]

Hello.  Does anyone have a processing protocol for formalin fixed decalcified
rat bones using an automated processor?  I've been doing it manually with a
vacuum oven and it takes 14-16 hours to complete.  I don't have a vacuumed
section on my automatic processor.

Also any tips besides what I've read in the archives for immunohistochemistry?

Thanks so much.

Michelle B. Titunick

PhD Candidate, Anatomy
The Pennsylvania State University College of Medicine
mbt137 at psu.edu
(973) 715-9831