[Histonet] malarial pigment

Paula Keene Pierce paula at excaliburpathology.com
Tue Mar 7 10:03:25 CST 2017

Lee Luna's third edition.
Page 43, Method II
1. Deparaffinize and hydrate to water.2. Wash in distilled water.3. Immerse slides in 100 ml of 70% alcohol, to which has been added 2 ml of 28% ammonium hydroxide, for 3 hours.4. Rinse in water.5. Rinse in 1% glacial acetic acid.6. Wash well in distilled water and stain as desired. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953FAX 405-759-7513www.excaliburpathology.com 

    On Tuesday, March 7, 2017 9:46 AM, "Sanders, Jeanine (CDC/OID/NCEZID) via Histonet" <histonet at lists.utsouthwestern.edu> wrote:

 Good morning!

Does anyone know of a method using ammonium hydroxide to remove malarial pigment from tissue sections?


Jeanine H. Sanders
Infectious Diseases Pathology Branch
Centers for Disease Control and Prevention
1600 Clifton Rd., NE MS-G32
Atlanta, GA 30329

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