From akemiat3377 at gmail.com Wed Mar 1 07:23:16 2017 From: akemiat3377 at gmail.com (Eileen Akemi Allison) Date: Wed, 1 Mar 2017 05:23:16 -0800 Subject: [Histonet] Cassette Printers Message-ID: Good morning Histoland! Our department is looking into purchasing a new cassette printer which has the capability of interfacing with our AP Easy LIS System. I am currently demoing a General Data ID/Positive CL-01 Laser Cassette Printer and label printer system. We are a fairly small lab with limited space so a small foot print is essential. We also do not need a multiple magazine. I would love to hear your comments, good, bad or indifferent? Thanks in advance Akemi Allison BS, HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants Laboratory 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 W: Email: aallison at montereygi.com H: Email: akemiat3377 at gmail.com From akemiat3377 at gmail.com Wed Mar 1 07:30:35 2017 From: akemiat3377 at gmail.com (Eileen Akemi Allison) Date: Wed, 1 Mar 2017 05:30:35 -0800 Subject: [Histonet] cassette printer additional request Message-ID: Hi Again: I forgot to mention I am open to investigate other Cassette Printers other than the General Data Printer? AlOf course, price, and dependability is a big factor. Would love to hear your comments, good, bad or indifferent... Thanks in again! Akemi Allison BS, HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants Laboratory 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 W: Email: aallison at montereygi.com H: Email: akemiat3377 at gmail.com From lblazek at digestivespecialists.com Wed Mar 1 08:50:13 2017 From: lblazek at digestivespecialists.com (Blazek, Linda) Date: Wed, 1 Mar 2017 09:50:13 -0500 Subject: [Histonet] cassette printer additional request References: Message-ID: <5A2BD13465E061429D6455C8D6B40E39198A4479BB@IBMB7Exchange.digestivespecialists.com> We have the Primera Signature Cassette printer and like it a lot. Support is excellent but we haven't needed it much. Set up is easy. Printing is very good. We also have their slide printer that is interfaced with our LIS. It's sold by Creative Waste Solutions. http://cwsincorp.com/ If you'd like any questions answered feel free to contact me. Linda Blazek HT (ASCP) Pathology Lab Manager GI Pathology of Dayton Digestive Specialists, Inc Phone: (937) 396-2623 Email: lblazek at digestivespecialists.com -----Original Message----- From: Eileen Akemi Allison via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, March 01, 2017 8:31 AM To: Histonet Subject: [Histonet] cassette printer additional request Hi Again: I forgot to mention I am open to investigate other Cassette Printers other than the General Data Printer? AlOf course, price, and dependability is a big factor. Would love to hear your comments, good, bad or indifferent... Thanks in again! Akemi Allison BS, HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants Laboratory 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 W: Email: aallison at montereygi.com H: Email: akemiat3377 at gmail.com _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Lisa.White3 at va.gov Wed Mar 1 12:32:28 2017 From: Lisa.White3 at va.gov (White, Lisa M.) Date: Wed, 1 Mar 2017 18:32:28 +0000 Subject: [Histonet] : Recycled reagents in VIP processor Message-ID: <6D9E570180A7FC4A8C972BBAF6C2B99202773805@VAPNWMSGD52S07.vha.med.va.gov> We have recycled here for over 14 years. The cost of the recycling unit is not small. It will take a while to recoup the cost. We have found that xylene recycles well. The substitutes are not infinitely recyclable. The biggest savings is that you are not paying to have drums of xylene picked up as hazardous waste. That is a savings in itself. Yes you are pouring into a 'dirty carboy' and loading and unloading onto a machine, but you are either doing that or pouring dirty product into a bottle to be sent for pick up as well as placing new in the processor carboys. Either way you have to handle product. If xylene or other solvent are out of range when your monitors are performed then the lab you work at must improve the ventilation as well as possibly provide chemical filter apparatus to be utilized by the staff. Just one more perspective. From john.frazier at roche.com Wed Mar 1 12:43:29 2017 From: john.frazier at roche.com (Frazier, John) Date: Wed, 1 Mar 2017 13:43:29 -0500 Subject: [Histonet] Histonet Digest, Vol 160, Issue 1 In-Reply-To: References: Message-ID: One last statement about recycling Xylene and Alcohol and I do not have a dog in the fight. *Many labs I visit have recyclers and many of those do not use them any more due to poor or inconsistent quality of the end product and overall disillusionment of the money or time they thought they would save.* I am not telling labs not to recycle but if they ask me, I tell them what I have seen and give them some of the data around the process measurements we have collected. Some variables that are critical in gauging the value-add or non value-add are: - Cost of Waste disposal per gallon - Fully Burdened Salary of the lab individual/s that currently handle reagent changes (H&E stainer, Tissue Processor) vs. that of lab individual/s that will be or are handling the the recyclable reagents - Time to retrieve fresh reagents vs. time to move the recyclable reagent to and from the recycler. - Cost of PPE, i.e. respirator mask, gloves - Cost per square foot of Storage space for Hazardous waste vs. recycler foot print. This is an opportunity cost that recycling may provide for other types of storage. - Time to make dilutions of alcohol (100% to 95%, 85%,etc.) - Time to measure the concentrations of the recycled alcohol - Time for corrections if the concentrations is too low or too high - Is there a xylene substitute or instrument that does not require xylene and or alcohol - Others Your have to be objective about the process measurements of making the decision. What am I gaining vs. what am getting vs. what am I giving up. On Wed, Mar 1, 2017 at 1:00 PM, wrote: > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > Today's Topics: > > 1. Re: Recycled reagents in VIP processor (Frazier, John) > 2. Leica CM1950 (Clements, Mary Ann) > 3. Re: Recycled reagents in VIP processor (Margaryan, Naira) > 4. Re: Recycled reagents in VIP processor (Gareth Davis) > 5. Re: Histonet Digest, Vol 159, Issue 24 (Joanne Clark) > 6. Cassette Printers (Eileen Akemi Allison) > 7. cassette printer additional request (Eileen Akemi Allison) > 8. Re: cassette printer additional request (Blazek, Linda) > > > ---------- Forwarded message ---------- > From: "Frazier, John" > To: Jay Lundgren > Cc: "Margaryan, Naira" , Gareth Davis < > garethdavisyuma at gmail.com>, "histonet at lists.utsouthwestern.edu" < > histonet at lists.utsouthwestern.edu>, "naira.margaryan at hsc.wvu.edu" < > naira.margaryan at hsc.wvu.edu> > Bcc: > Date: Tue, 28 Feb 2017 14:30:46 -0500 > Subject: Re: [Histonet] Recycled reagents in VIP processor > As a six sigma consultant to histology laboratories, it has been my > experience that recycling xylene and alcohol overall is not a cost > saver. When you factor in both capital dollars and operational > dollars, the savings is neutral. In addition to the neutral cost in > recycling, you have to concern yourself with the quality of your in > product on a daily basis. The last piece in the equation is the > handling of Xylene with multiple touch points in between. A movement > within the histology world has begun with handling xylene (hazard > waste) as little as possible and/or reducing or eliminating its use > where possible. > > Sent from my iPhone > > > On Feb 27, 2017, at 6:25 PM, Jay Lundgren wrote: > > > > When people say they are saving money by recycling reagents I always > wonder > > if they are including tech time (to run the still) in their calculations. > > > > Sincerely, > > > > Jay A. Lundgren, M.S., HTL (ASCP) > > > > On Wed, Feb 22, 2017 at 2:32 PM, Margaryan, Naira via Histonet < > > histonet at lists.utsouthwestern.edu> wrote: > > > >> WOULD LIKE TO KNOW AS WELL - IT IS WASTE OF MONEY.... > >> > >> Thanks, > >> Naira > >> > >> > >> Ranked nationally in all 10 pediatric specialties by U.S. News & World > >> Report (LCHOC Ver 1.0) > >> > >> > >> This message contains confidential information and is intended only for > >> the individual named. If you are not the named addressee you should not > >> disseminate, distribute or copy this e-mail. Please notify the sender > >> immediately by e-mail if you have received this e-mail by mistake and > >> delete this e-mail from your system. E-mail transmission cannot be > >> guaranteed to be secure or error-free as information could be > intercepted, > >> corrupted, lost, destroyed, arrive late or incomplete, or contain > viruses. > >> The sender therefore does not accept liability for any errors or > omissions > >> in the contents of this message, which arise as a result of e-mail > >> transmission. If verification is required please request a hard-copy > >> version. (LCHOC VER 1.0) > >> > >> ________________________________________ > >> From: Gareth Davis via Histonet [histonet at lists.utsouthwestern.edu] > >> Sent: Wednesday, February 22, 2017 2:23 PM > >> To: histonet at lists.utsouthwestern.edu > >> Subject: [Histonet] Recycled reagents in VIP processor > >> > >> Hi, > >> I was always told not to use recycled reagents, i.e. Alcohol and > Xylene, in > >> processors. I am using a VIP 300, refurbished, and I would rather not > use > >> recycled reagents in it. But, during the last CAP inspection they > >> suggested I use the recycled to save money. And now my administration > >> wants to cut cost. Just wanted to know what labs were doing. > >> Thanks, > >> > >> > >> -- > >> Ms. Gareth B. Davis, B.S., HT, QIHC (ASCP)cm > >> Yuma Gastroenterology > >> Yuma, AZ 85364 > >> 928-248-5259 > >> _______________________________________________ > >> Histonet mailing list > >> Histonet at lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> _______________________________________________ > >> Histonet mailing list > >> Histonet at lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> > > > > > > > ---------- Forwarded message ---------- > From: "Clements, Mary Ann" > To: "'histonet at lists.utsouthwestern.edu'" < > histonet at lists.utsouthwestern.edu> > Cc: > Bcc: > Date: Tue, 28 Feb 2017 19:50:49 +0000 > Subject: [Histonet] Leica CM1950 > We are interested in purchasing the Leica CM 1950 cryostat. Would love to > hear your comments, good, bad or indifferent... > Thanks in advance! > > Mary Ann Clements, B.S. > Biorepository Manager > Department of Pathology & Anatomy > Eastern Virginia Medical School > 700 West Olney Road > Lewis Hall, Room 3011 > Norfolk, VA 23507 > > email: clemenma at evms.edu > phone: (757) 446-7910 > fax: (757) 446-7059 > > > > > ---------- Forwarded message ---------- > From: "Margaryan, Naira" > To: "Frazier, John" , Jay Lundgren < > jaylundgren at gmail.com> > Cc: "Margaryan, Naira" , Gareth Davis < > garethdavisyuma at gmail.com>, "histonet at lists.utsouthwestern.edu" < > histonet at lists.utsouthwestern.edu> > Bcc: > Date: Tue, 28 Feb 2017 20:39:10 +0000 > Subject: Re: [Histonet] Recycled reagents in VIP processor > Absolutely agreed! > > Naira > > -----Original Message----- > From: Frazier, John [mailto:john.frazier at roche.com] > Sent: Tuesday, February 28, 2017 2:31 PM > To: Jay Lundgren > Cc: Margaryan, Naira ; Gareth Davis < > garethdavisyuma at gmail.com>; histonet at lists.utsouthwestern.edu; Margaryan, > Naira > Subject: Re: [Histonet] Recycled reagents in VIP processor > > As a six sigma consultant to histology laboratories, it has been my > experience that recycling xylene and alcohol overall is not a cost saver. > When you factor in both capital dollars and operational dollars, the > savings is neutral. In addition to the neutral cost in recycling, you have > to concern yourself with the quality of your in product on a daily basis. > The last piece in the equation is the handling of Xylene with multiple > touch points in between. A movement within the histology world has begun > with handling xylene (hazard > waste) as little as possible and/or reducing or eliminating its use where > possible. > > Sent from my iPhone > > > On Feb 27, 2017, at 6:25 PM, Jay Lundgren wrote: > > > > When people say they are saving money by recycling reagents I always > > wonder if they are including tech time (to run the still) in their > calculations. > > > > Sincerely, > > > > Jay A. Lundgren, M.S., HTL (ASCP) > > > > On Wed, Feb 22, 2017 at 2:32 PM, Margaryan, Naira via Histonet < > > histonet at lists.utsouthwestern.edu> wrote: > > > >> WOULD LIKE TO KNOW AS WELL - IT IS WASTE OF MONEY.... > >> > >> Thanks, > >> Naira > >> > >> > >> Ranked nationally in all 10 pediatric specialties by U.S. News & > >> World Report (LCHOC Ver 1.0) > >> > >> > >> This message contains confidential information and is intended only > >> for the individual named. If you are not the named addressee you > >> should not disseminate, distribute or copy this e-mail. Please notify > >> the sender immediately by e-mail if you have received this e-mail by > >> mistake and delete this e-mail from your system. E-mail transmission > >> cannot be guaranteed to be secure or error-free as information could > >> be intercepted, corrupted, lost, destroyed, arrive late or incomplete, > or contain viruses. > >> The sender therefore does not accept liability for any errors or > >> omissions in the contents of this message, which arise as a result of > >> e-mail transmission. If verification is required please request a > >> hard-copy version. (LCHOC VER 1.0) > >> > >> ________________________________________ > >> From: Gareth Davis via Histonet [histonet at lists.utsouthwestern.edu] > >> Sent: Wednesday, February 22, 2017 2:23 PM > >> To: histonet at lists.utsouthwestern.edu > >> Subject: [Histonet] Recycled reagents in VIP processor > >> > >> Hi, > >> I was always told not to use recycled reagents, i.e. Alcohol and > >> Xylene, in processors. I am using a VIP 300, refurbished, and I > >> would rather not use recycled reagents in it. But, during the last > >> CAP inspection they suggested I use the recycled to save money. And > >> now my administration wants to cut cost. Just wanted to know what labs > were doing. > >> Thanks, > >> > >> > >> -- > >> Ms. Gareth B. Davis, B.S., HT, QIHC (ASCP)cm Yuma Gastroenterology > >> Yuma, AZ 85364 > >> 928-248-5259 > >> _______________________________________________ > >> Histonet mailing list > >> Histonet at lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> _______________________________________________ > >> Histonet mailing list > >> Histonet at lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> > > > > > ---------- Forwarded message ---------- > From: Gareth Davis > To: "Margaryan, Naira" > Cc: "Frazier, John" , Jay Lundgren < > jaylundgren at gmail.com>, "Margaryan, Naira" , > "histonet at lists.utsouthwestern.edu" > Bcc: > Date: Tue, 28 Feb 2017 13:57:24 -0700 > Subject: Re: [Histonet] Recycled reagents in VIP processor > When using Xylene in a lab, it's my opinion that the savings is associated > with the size of the lab. I am in a very small lab, where I am the only > Histotech. I recycle both alcohol and Xylene and monitor both closely. > For me, it would cost effective to recycle and use as much as possible, > instead of purchasing fresh. But, I agree, if it's a larger lab it may not > be as cost effective. Also, if the lab is in the position to order a > substitute for Xylene, where it would be cheaper, than that seems to be the > optimum thing to do. > > On Tue, Feb 28, 2017 at 1:39 PM, Margaryan, Naira < > naira.margaryan at hsc.wvu.edu> wrote: > > > Absolutely agreed! > > > > Naira > > > > -----Original Message----- > > From: Frazier, John [mailto:john.frazier at roche.com] > > Sent: Tuesday, February 28, 2017 2:31 PM > > To: Jay Lundgren > > Cc: Margaryan, Naira ; Gareth Davis < > > garethdavisyuma at gmail.com>; histonet at lists.utsouthwestern.edu; > Margaryan, > > Naira > > Subject: Re: [Histonet] Recycled reagents in VIP processor > > > > As a six sigma consultant to histology laboratories, it has been my > > experience that recycling xylene and alcohol overall is not a cost saver. > > When you factor in both capital dollars and operational dollars, the > > savings is neutral. In addition to the neutral cost in recycling, you > have > > to concern yourself with the quality of your in product on a daily basis. > > The last piece in the equation is the handling of Xylene with multiple > > touch points in between. A movement within the histology world has begun > > with handling xylene (hazard > > waste) as little as possible and/or reducing or eliminating its use where > > possible. > > > > Sent from my iPhone > > > > > On Feb 27, 2017, at 6:25 PM, Jay Lundgren > wrote: > > > > > > When people say they are saving money by recycling reagents I always > > > wonder if they are including tech time (to run the still) in their > > calculations. > > > > > > Sincerely, > > > > > > Jay A. Lundgren, M.S., HTL (ASCP) > > > > > > On Wed, Feb 22, 2017 at 2:32 PM, Margaryan, Naira via Histonet < > > > histonet at lists.utsouthwestern.edu> wrote: > > > > > >> WOULD LIKE TO KNOW AS WELL - IT IS WASTE OF MONEY.... > > >> > > >> Thanks, > > >> Naira > > >> > > >> > > >> Ranked nationally in all 10 pediatric specialties by U.S. News & > > >> World Report (LCHOC Ver 1.0) > > >> > > >> > > >> This message contains confidential information and is intended only > > >> for the individual named. If you are not the named addressee you > > >> should not disseminate, distribute or copy this e-mail. Please notify > > >> the sender immediately by e-mail if you have received this e-mail by > > >> mistake and delete this e-mail from your system. E-mail transmission > > >> cannot be guaranteed to be secure or error-free as information could > > >> be intercepted, corrupted, lost, destroyed, arrive late or incomplete, > > or contain viruses. > > >> The sender therefore does not accept liability for any errors or > > >> omissions in the contents of this message, which arise as a result of > > >> e-mail transmission. If verification is required please request a > > >> hard-copy version. (LCHOC VER 1.0) > > >> > > >> ________________________________________ > > >> From: Gareth Davis via Histonet [histonet at lists.utsouthwestern.edu] > > >> Sent: Wednesday, February 22, 2017 2:23 PM > > >> To: histonet at lists.utsouthwestern.edu > > >> Subject: [Histonet] Recycled reagents in VIP processor > > >> > > >> Hi, > > >> I was always told not to use recycled reagents, i.e. Alcohol and > > >> Xylene, in processors. I am using a VIP 300, refurbished, and I > > >> would rather not use recycled reagents in it. But, during the last > > >> CAP inspection they suggested I use the recycled to save money. And > > >> now my administration wants to cut cost. Just wanted to know what > labs > > were doing. > > >> Thanks, > > >> > > >> > > >> -- > > >> Ms. Gareth B. Davis, B.S., HT, QIHC (ASCP)cm Yuma Gastroenterology > > >> Yuma, AZ 85364 > > >> 928-248-5259 > > >> _______________________________________________ > > >> Histonet mailing list > > >> Histonet at lists.utsouthwestern.edu > > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >> _______________________________________________ > > >> Histonet mailing list > > >> Histonet at lists.utsouthwestern.edu > > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >> > > > > > > > > > -- > Ms. Gareth B. Davis, B.S., HT, QIHC (ASCP)cm > Yuma Gastroenterology > Yuma, AZ 85364 > 928-248-5259 > > > > ---------- Forwarded message ---------- > From: Joanne Clark > To: "histonet at lists.utsouthwestern.edu" > > Cc: > Bcc: > Date: Tue, 28 Feb 2017 21:37:10 +0000 > Subject: Re: [Histonet] Histonet Digest, Vol 159, Issue 24 > > > > Hi Allison, I talked to my safety guy and there isn't anything specific. > They are telling you that because there is a fire hazard warning on > formalin cubes, but we know that the change of it catching fire is slim to > nil. They want you to use the same standards as with alcohol and xylene (1 > gallon per 100 square feet). It is all so dependent on who you have > inspecting you and what their background is. > > Joanne Clark, HT > Pathology Consultants of New Mexico > > ---------------------------------------------------------------------- > > Message: 1 > Date: Mon, 27 Feb 2017 19:26:54 +0000 > From: "Eck, Allison" > To: "'histonet at lists.utsouthwestern.edu'" > > Subject: [Histonet] square footage for formalin > Message-ID: > <4ED8C96A8F20FC4F883A92E2A0A0D64A97341B84 at DH-MAIL01.dhorg.org> > Content-Type: text/plain; charset="us-ascii" > > Good afternoon, > We have inspectors here and they are questioning the size of the room in > the operating room where they keep their 5 gallon cube. Does anyone know > of any square footage requirements for a room that where formalin is kept > and used? > > Thank you in advance > Allison > > Allison Eck, HTL(ASCP)cm,QLS, AHI(AMT) > Lead Tech Histology > Doylestown Hospital > 595 W State St > Doylestown, PA 18901 > 215-345-2264 > aeck at dh.org > > > > > ------------------------------ > > > Disclaimer: This electronic message may contain information that is > Proprietary, Confidential, or legally privileged or protected. It is > intended only for the use of the individual(s) and entity named in the > message. If you are not an intended recipient of this message, please > notify the sender immediately and delete the material from your computer. > Do not deliver, distribute or copy this message and do not disclose its > contents or take any action in reliance on the information it contains. > > > > > ---------- Forwarded message ---------- > From: Eileen Akemi Allison > To: Histonet > Cc: > Bcc: > Date: Wed, 1 Mar 2017 05:23:16 -0800 > Subject: [Histonet] Cassette Printers > Good morning Histoland! > > Our department is looking into purchasing a new cassette printer which has > the capability of interfacing with our AP Easy LIS System. I am currently > demoing a General Data ID/Positive CL-01 Laser Cassette Printer and label > printer system. We are a fairly small lab with limited space so a small > foot print is essential. We also do not need a multiple magazine. > > I would love to hear your comments, good, bad or indifferent? > > Thanks in advance > > Akemi Allison BS, HT/HTL (ASCP) > Pathology Manager > Monterey Bay GI Consultants Laboratory > 23 Upper Ragsdale Drive, Suite 200 > Monterey, CA 93940 > W: Email: aallison at montereygi.com > H: Email: akemiat3377 at gmail.com > > > > > ---------- Forwarded message ---------- > From: Eileen Akemi Allison > To: Histonet > Cc: > Bcc: > Date: Wed, 1 Mar 2017 05:30:35 -0800 > Subject: [Histonet] cassette printer additional request > Hi Again: > > I forgot to mention I am open to investigate other Cassette Printers other > than the General Data Printer? AlOf course, price, and dependability is a > big factor. Would love to hear your comments, good, bad or indifferent... > > Thanks in again! > > Akemi Allison BS, HT/HTL (ASCP) > Pathology Manager > Monterey Bay GI Consultants Laboratory > 23 Upper Ragsdale Drive, Suite 200 > Monterey, CA 93940 > W: Email: aallison at montereygi.com > H: Email: akemiat3377 at gmail.com > > > > > ---------- Forwarded message ---------- > From: "Blazek, Linda" > To: Eileen Akemi Allison , " > histonet at lists.utsouthwestern.edu" > Cc: > Bcc: > Date: Wed, 1 Mar 2017 09:50:13 -0500 > Subject: Re: [Histonet] cassette printer additional request > > We have the Primera Signature Cassette printer and like it a lot. Support > is excellent but we haven't needed it much. Set up is easy. Printing is > very good. We also have their slide printer that is interfaced with our > LIS. It's sold by Creative Waste Solutions. > http://cwsincorp.com/ > If you'd like any questions answered feel free to contact me. > > Linda Blazek HT (ASCP) > Pathology Lab Manager > GI Pathology of Dayton > Digestive Specialists, Inc > Phone: (937) 396-2623 > Email: lblazek at digestivespecialists.com > > > -----Original Message----- > From: Eileen Akemi Allison via Histonet [mailto:histonet at lists. > utsouthwestern.edu] > Sent: Wednesday, March 01, 2017 8:31 AM > To: Histonet > Subject: [Histonet] cassette printer additional request > > Hi Again: > > I forgot to mention I am open to investigate other Cassette Printers other > than the General Data Printer? AlOf course, price, and dependability is a > big factor. Would love to hear your comments, good, bad or indifferent... > > Thanks in again! > > Akemi Allison BS, HT/HTL (ASCP) > Pathology Manager > Monterey Bay GI Consultants Laboratory > 23 Upper Ragsdale Drive, Suite 200 > Monterey, CA 93940 > W: Email: aallison at montereygi.com > H: Email: akemiat3377 at gmail.com > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- John S. Frazier, MT(ASCP), MBA, LSSBB Senior Manager, RMS Consulting Roche Diagnostics Corporation Mobile: 1-520-305-5030 Fax: 1-520-229-6819 john.frazier at roche.com From jaylundgren at gmail.com Wed Mar 1 12:58:27 2017 From: jaylundgren at gmail.com (Jay Lundgren) Date: Wed, 1 Mar 2017 12:58:27 -0600 Subject: [Histonet] Recycled reagents in VIP processor In-Reply-To: References: <-2110263303007881997@unknownmsgid> Message-ID: Yes, it was my suspicion that if you add in tech time , there is not a savings. The histotech is, after all, the most expensive piece of equipment you have in the lab. In my personal experience, most labs that start recycling don't keep it up, when they discover it doesn't really save them money. Plus, I think recycling xylene is a tremendous fire risk. Maybe they are safer nowadays, but I knew a lab superintendent in the Air Force who had won an Airman's Medal (the highest non-combat award for heroism in the Air Force) for dragging a co-worker from a flaming room when the xylene still blew up. I talked to the airman he saved, who described being absolutely unable to stand up on the ultra-slippery floor covered in a slurry of paraffin and xylene, and totally resigned to burning to death. Until the heroic superintendent swam/wriggled across the burning floor to drag him to safety. Something to think about the next time you recycle your xylene. On Tue, Feb 28, 2017 at 2:57 PM, Gareth Davis wrote: > When using Xylene in a lab, it's my opinion that the savings is associated > with the size of the lab. I am in a very small lab, where I am the only > Histotech. I recycle both alcohol and Xylene and monitor both closely. > For me, it would cost effective to recycle and use as much as possible, > instead of purchasing fresh. But, I agree, if it's a larger lab it may not > be as cost effective. Also, if the lab is in the position to order a > substitute for Xylene, where it would be cheaper, than that seems to be the > optimum thing to do. > > On Tue, Feb 28, 2017 at 1:39 PM, Margaryan, Naira < > naira.margaryan at hsc.wvu.edu> wrote: > >> Absolutely agreed! >> >> Naira >> >> -----Original Message----- >> From: Frazier, John [mailto:john.frazier at roche.com] >> Sent: Tuesday, February 28, 2017 2:31 PM >> To: Jay Lundgren >> Cc: Margaryan, Naira ; Gareth Davis < >> garethdavisyuma at gmail.com>; histonet at lists.utsouthwestern.edu; >> Margaryan, Naira >> Subject: Re: [Histonet] Recycled reagents in VIP processor >> >> As a six sigma consultant to histology laboratories, it has been my >> experience that recycling xylene and alcohol overall is not a cost saver. >> When you factor in both capital dollars and operational dollars, the >> savings is neutral. In addition to the neutral cost in recycling, you have >> to concern yourself with the quality of your in product on a daily basis. >> The last piece in the equation is the handling of Xylene with multiple >> touch points in between. A movement within the histology world has begun >> with handling xylene (hazard >> waste) as little as possible and/or reducing or eliminating its use where >> possible. >> >> Sent from my iPhone >> >> > On Feb 27, 2017, at 6:25 PM, Jay Lundgren >> wrote: >> > >> > When people say they are saving money by recycling reagents I always >> > wonder if they are including tech time (to run the still) in their >> calculations. >> > >> > Sincerely, >> > >> > Jay A. Lundgren, M.S., HTL (ASCP) >> > >> > On Wed, Feb 22, 2017 at 2:32 PM, Margaryan, Naira via Histonet < >> > histonet at lists.utsouthwestern.edu> wrote: >> > >> >> WOULD LIKE TO KNOW AS WELL - IT IS WASTE OF MONEY.... >> >> >> >> Thanks, >> >> Naira >> >> >> >> >> >> Ranked nationally in all 10 pediatric specialties by U.S. News & >> >> World Report (LCHOC Ver 1.0) >> >> >> >> >> >> This message contains confidential information and is intended only >> >> for the individual named. If you are not the named addressee you >> >> should not disseminate, distribute or copy this e-mail. Please notify >> >> the sender immediately by e-mail if you have received this e-mail by >> >> mistake and delete this e-mail from your system. E-mail transmission >> >> cannot be guaranteed to be secure or error-free as information could >> >> be intercepted, corrupted, lost, destroyed, arrive late or incomplete, >> or contain viruses. >> >> The sender therefore does not accept liability for any errors or >> >> omissions in the contents of this message, which arise as a result of >> >> e-mail transmission. If verification is required please request a >> >> hard-copy version. (LCHOC VER 1.0) >> >> >> >> ________________________________________ >> >> From: Gareth Davis via Histonet [histonet at lists.utsouthwestern.edu] >> >> Sent: Wednesday, February 22, 2017 2:23 PM >> >> To: histonet at lists.utsouthwestern.edu >> >> Subject: [Histonet] Recycled reagents in VIP processor >> >> >> >> Hi, >> >> I was always told not to use recycled reagents, i.e. Alcohol and >> >> Xylene, in processors. I am using a VIP 300, refurbished, and I >> >> would rather not use recycled reagents in it. But, during the last >> >> CAP inspection they suggested I use the recycled to save money. And >> >> now my administration wants to cut cost. Just wanted to know what >> labs were doing. >> >> Thanks, >> >> >> >> >> >> -- >> >> Ms. Gareth B. Davis, B.S., HT, QIHC (ASCP)cm Yuma Gastroenterology >> >> Yuma, AZ 85364 >> >> 928-248-5259 >> >> _______________________________________________ >> >> Histonet mailing list >> >> Histonet at lists.utsouthwestern.edu >> >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> >> Histonet mailing list >> >> Histonet at lists.utsouthwestern.edu >> >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> > >> > > > > -- > Ms. Gareth B. Davis, B.S., HT, QIHC (ASCP)cm > Yuma Gastroenterology > Yuma, AZ 85364 > 928-248-5259 <(928)%20248-5259> > > From garethdavisyuma at gmail.com Wed Mar 1 13:20:54 2017 From: garethdavisyuma at gmail.com (Gareth Davis) Date: Wed, 1 Mar 2017 12:20:54 -0700 Subject: [Histonet] : Recycled reagents in VIP processor In-Reply-To: <6D9E570180A7FC4A8C972BBAF6C2B99202773805@VAPNWMSGD52S07.vha.med.va.gov> References: <6D9E570180A7FC4A8C972BBAF6C2B99202773805@VAPNWMSGD52S07.vha.med.va.gov> Message-ID: Thank you for your perspective. All input is helpful in our decision. Gareth On Wed, Mar 1, 2017 at 11:32 AM, White, Lisa M. via Histonet < histonet at lists.utsouthwestern.edu> wrote: > We have recycled here for over 14 years. The cost of the recycling unit > is not small. It will take a while to recoup the cost. We have found that > xylene recycles well. The substitutes are not infinitely recyclable. The > biggest savings is that you are not paying to have drums of xylene picked > up as hazardous waste. That is a savings in itself. Yes you are pouring > into a 'dirty carboy' and loading and unloading onto a machine, but you are > either doing that or pouring dirty product into a bottle to be sent for > pick up as well as placing new in the processor carboys. Either way you > have to handle product. > If xylene or other solvent are out of range when your monitors are > performed then the lab you work at must improve the ventilation as well as > possibly provide chemical filter apparatus to be utilized by the staff. > > Just one more perspective. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Ms. Gareth B. Davis, B.S., HT, QIHC (ASCP)cm Yuma Gastroenterology Yuma, AZ 85364 928-248-5259 From koellingr at comcast.net Wed Mar 1 13:23:26 2017 From: koellingr at comcast.net (koellingr at comcast.net) Date: Wed, 1 Mar 2017 19:23:26 +0000 (UTC) Subject: [Histonet] Histonet Digest, Vol 160, Issue 1 In-Reply-To: References: Message-ID: <301539714.124001725.1488396205931.JavaMail.zimbra@comcast.net> All, I think John Frazier is exactly spot-on with his e-mail. This is not a single variable equation solved easily. It is complex and not so easy as it might seem for a recycler sales pitch. I am FOR recycling and the environment whenever it is at all possible in any situation but there is not an easy answer that covers every lab. One thing that John alluded to, and has stuck with me for years and years, is the concept of "poor or inconsistent quality of the end product" . I've read numerous e-mails of the xylene coming off a recycler as being "purer" than the original. I just can't wrap my mind around that when multi-billion dollar fractional distillation mega-complexes will supply alcohol or xylene and now a lab recycler is making it "purer and better" than when purchased? When I was at a biotech company a while back we followed many lots of xylene and reagent alcohol from purchase to use to recycling by sending samples to outside chemistry analysis labs doing high-level testing of purity and contaminations. While in general the recycler had worked OK it was not producing product chemically consistent or optimally pure. It was "OK". While histo-pathology labored in a world of H&E sections ONLY, possibly those re-used reagents were and still are very, very acceptable. But I wonder about when that tissue is going to be used for very sensitive IHC testing (Her2) or PCR to look at mutations or FISH to look at different lymphoma's? Where tissue processing and quality is absolutely critical. I'm FOR recycling if possible but it is not a universal panacea in my opinion. What about the patient tissue for sophisticated testing? Ray Koelling Lecture Faculty UW School of Medicine-WWAMI, Spokane ----- Original Message ----- From: "John via Histonet Frazier" To: "Histonet (histonet at lists.utsouthwestern.edu)" Sent: Wednesday, March 1, 2017 10:43:29 AM Subject: Re: [Histonet] Histonet Digest, Vol 160, Issue 1 One last statement about recycling Xylene and Alcohol and I do not have a dog in the fight. *Many labs I visit have recyclers and many of those do not use them any more due to poor or inconsistent quality of the end product and overall disillusionment of the money or time they thought they would save.* I am not telling labs not to recycle but if they ask me, I tell them what I have seen and give them some of the data around the process measurements we have collected. Some variables that are critical in gauging the value-add or non value-add are: - Cost of Waste disposal per gallon - Fully Burdened Salary of the lab individual/s that currently handle reagent changes (H&E stainer, Tissue Processor) vs. that of lab individual/s that will be or are handling the the recyclable reagents - Time to retrieve fresh reagents vs. time to move the recyclable reagent to and from the recycler. - Cost of PPE, i.e. respirator mask, gloves - Cost per square foot of Storage space for Hazardous waste vs. recycler foot print. This is an opportunity cost that recycling may provide for other types of storage. - Time to make dilutions of alcohol (100% to 95%, 85%,etc.) - Time to measure the concentrations of the recycled alcohol - Time for corrections if the concentrations is too low or too high - Is there a xylene substitute or instrument that does not require xylene and or alcohol - Others Your have to be objective about the process measurements of making the decision. What am I gaining vs. what am getting vs. what am I giving up. On Wed, Mar 1, 2017 at 1:00 PM, wrote: > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > Today's Topics: > > 1. Re: Recycled reagents in VIP processor (Frazier, John) > 2. Leica CM1950 (Clements, Mary Ann) > 3. Re: Recycled reagents in VIP processor (Margaryan, Naira) > 4. Re: Recycled reagents in VIP processor (Gareth Davis) > 5. Re: Histonet Digest, Vol 159, Issue 24 (Joanne Clark) > 6. Cassette Printers (Eileen Akemi Allison) > 7. cassette printer additional request (Eileen Akemi Allison) > 8. Re: cassette printer additional request (Blazek, Linda) > > > ---------- Forwarded message ---------- > From: "Frazier, John" > To: Jay Lundgren > Cc: "Margaryan, Naira" , Gareth Davis < > garethdavisyuma at gmail.com>, "histonet at lists.utsouthwestern.edu" < > histonet at lists.utsouthwestern.edu>, "naira.margaryan at hsc.wvu.edu" < > naira.margaryan at hsc.wvu.edu> > Bcc: > Date: Tue, 28 Feb 2017 14:30:46 -0500 > Subject: Re: [Histonet] Recycled reagents in VIP processor > As a six sigma consultant to histology laboratories, it has been my > experience that recycling xylene and alcohol overall is not a cost > saver. When you factor in both capital dollars and operational > dollars, the savings is neutral. In addition to the neutral cost in > recycling, you have to concern yourself with the quality of your in > product on a daily basis. The last piece in the equation is the > handling of Xylene with multiple touch points in between. A movement > within the histology world has begun with handling xylene (hazard > waste) as little as possible and/or reducing or eliminating its use > where possible. > > Sent from my iPhone > > > On Feb 27, 2017, at 6:25 PM, Jay Lundgren wrote: > > > > When people say they are saving money by recycling reagents I always > wonder > > if they are including tech time (to run the still) in their calculations. > > > > Sincerely, > > > > Jay A. Lundgren, M.S., HTL (ASCP) > > > > On Wed, Feb 22, 2017 at 2:32 PM, Margaryan, Naira via Histonet < > > histonet at lists.utsouthwestern.edu> wrote: > > > >> WOULD LIKE TO KNOW AS WELL - IT IS WASTE OF MONEY.... > >> > >> Thanks, > >> Naira > >> > >> > >> Ranked nationally in all 10 pediatric specialties by U.S. News & World > >> Report (LCHOC Ver 1.0) > >> > >> > >> This message contains confidential information and is intended only for > >> the individual named. If you are not the named addressee you should not > >> disseminate, distribute or copy this e-mail. Please notify the sender > >> immediately by e-mail if you have received this e-mail by mistake and > >> delete this e-mail from your system. E-mail transmission cannot be > >> guaranteed to be secure or error-free as information could be > intercepted, > >> corrupted, lost, destroyed, arrive late or incomplete, or contain > viruses. > >> The sender therefore does not accept liability for any errors or > omissions > >> in the contents of this message, which arise as a result of e-mail > >> transmission. If verification is required please request a hard-copy > >> version. (LCHOC VER 1.0) > >> > >> ________________________________________ > >> From: Gareth Davis via Histonet [histonet at lists.utsouthwestern.edu] > >> Sent: Wednesday, February 22, 2017 2:23 PM > >> To: histonet at lists.utsouthwestern.edu > >> Subject: [Histonet] Recycled reagents in VIP processor > >> > >> Hi, > >> I was always told not to use recycled reagents, i.e. Alcohol and > Xylene, in > >> processors. I am using a VIP 300, refurbished, and I would rather not > use > >> recycled reagents in it. But, during the last CAP inspection they > >> suggested I use the recycled to save money. And now my administration > >> wants to cut cost. Just wanted to know what labs were doing. > >> Thanks, > >> > >> > >> -- > >> Ms. Gareth B. Davis, B.S., HT, QIHC (ASCP)cm > >> Yuma Gastroenterology > >> Yuma, AZ 85364 > >> 928-248-5259 > >> _______________________________________________ > >> Histonet mailing list > >> Histonet at lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> _______________________________________________ > >> Histonet mailing list > >> Histonet at lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> > > > > > > > ---------- Forwarded message ---------- > From: "Clements, Mary Ann" > To: "'histonet at lists.utsouthwestern.edu'" < > histonet at lists.utsouthwestern.edu> > Cc: > Bcc: > Date: Tue, 28 Feb 2017 19:50:49 +0000 > Subject: [Histonet] Leica CM1950 > We are interested in purchasing the Leica CM 1950 cryostat. Would love to > hear your comments, good, bad or indifferent... > Thanks in advance! > > Mary Ann Clements, B.S. > Biorepository Manager > Department of Pathology & Anatomy > Eastern Virginia Medical School > 700 West Olney Road > Lewis Hall, Room 3011 > Norfolk, VA 23507 > > email: clemenma at evms.edu > phone: (757) 446-7910 > fax: (757) 446-7059 > > > > > ---------- Forwarded message ---------- > From: "Margaryan, Naira" > To: "Frazier, John" , Jay Lundgren < > jaylundgren at gmail.com> > Cc: "Margaryan, Naira" , Gareth Davis < > garethdavisyuma at gmail.com>, "histonet at lists.utsouthwestern.edu" < > histonet at lists.utsouthwestern.edu> > Bcc: > Date: Tue, 28 Feb 2017 20:39:10 +0000 > Subject: Re: [Histonet] Recycled reagents in VIP processor > Absolutely agreed! > > Naira > > -----Original Message----- > From: Frazier, John [mailto:john.frazier at roche.com] > Sent: Tuesday, February 28, 2017 2:31 PM > To: Jay Lundgren > Cc: Margaryan, Naira ; Gareth Davis < > garethdavisyuma at gmail.com>; histonet at lists.utsouthwestern.edu; Margaryan, > Naira > Subject: Re: [Histonet] Recycled reagents in VIP processor > > As a six sigma consultant to histology laboratories, it has been my > experience that recycling xylene and alcohol overall is not a cost saver. > When you factor in both capital dollars and operational dollars, the > savings is neutral. In addition to the neutral cost in recycling, you have > to concern yourself with the quality of your in product on a daily basis. > The last piece in the equation is the handling of Xylene with multiple > touch points in between. A movement within the histology world has begun > with handling xylene (hazard > waste) as little as possible and/or reducing or eliminating its use where > possible. > > Sent from my iPhone > > > On Feb 27, 2017, at 6:25 PM, Jay Lundgren wrote: > > > > When people say they are saving money by recycling reagents I always > > wonder if they are including tech time (to run the still) in their > calculations. > > > > Sincerely, > > > > Jay A. Lundgren, M.S., HTL (ASCP) > > > > On Wed, Feb 22, 2017 at 2:32 PM, Margaryan, Naira via Histonet < > > histonet at lists.utsouthwestern.edu> wrote: > > > >> WOULD LIKE TO KNOW AS WELL - IT IS WASTE OF MONEY.... > >> > >> Thanks, > >> Naira > >> > >> > >> Ranked nationally in all 10 pediatric specialties by U.S. News & > >> World Report (LCHOC Ver 1.0) > >> > >> > >> This message contains confidential information and is intended only > >> for the individual named. If you are not the named addressee you > >> should not disseminate, distribute or copy this e-mail. Please notify > >> the sender immediately by e-mail if you have received this e-mail by > >> mistake and delete this e-mail from your system. E-mail transmission > >> cannot be guaranteed to be secure or error-free as information could > >> be intercepted, corrupted, lost, destroyed, arrive late or incomplete, > or contain viruses. > >> The sender therefore does not accept liability for any errors or > >> omissions in the contents of this message, which arise as a result of > >> e-mail transmission. If verification is required please request a > >> hard-copy version. (LCHOC VER 1.0) > >> > >> ________________________________________ > >> From: Gareth Davis via Histonet [histonet at lists.utsouthwestern.edu] > >> Sent: Wednesday, February 22, 2017 2:23 PM > >> To: histonet at lists.utsouthwestern.edu > >> Subject: [Histonet] Recycled reagents in VIP processor > >> > >> Hi, > >> I was always told not to use recycled reagents, i.e. Alcohol and > >> Xylene, in processors. I am using a VIP 300, refurbished, and I > >> would rather not use recycled reagents in it. But, during the last > >> CAP inspection they suggested I use the recycled to save money. And > >> now my administration wants to cut cost. Just wanted to know what labs > were doing. > >> Thanks, > >> > >> > >> -- > >> Ms. Gareth B. Davis, B.S., HT, QIHC (ASCP)cm Yuma Gastroenterology > >> Yuma, AZ 85364 > >> 928-248-5259 > >> _______________________________________________ > >> Histonet mailing list > >> Histonet at lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> _______________________________________________ > >> Histonet mailing list > >> Histonet at lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> > > > > > ---------- Forwarded message ---------- > From: Gareth Davis > To: "Margaryan, Naira" > Cc: "Frazier, John" , Jay Lundgren < > jaylundgren at gmail.com>, "Margaryan, Naira" , > "histonet at lists.utsouthwestern.edu" > Bcc: > Date: Tue, 28 Feb 2017 13:57:24 -0700 > Subject: Re: [Histonet] Recycled reagents in VIP processor > When using Xylene in a lab, it's my opinion that the savings is associated > with the size of the lab. I am in a very small lab, where I am the only > Histotech. I recycle both alcohol and Xylene and monitor both closely. > For me, it would cost effective to recycle and use as much as possible, > instead of purchasing fresh. But, I agree, if it's a larger lab it may not > be as cost effective. Also, if the lab is in the position to order a > substitute for Xylene, where it would be cheaper, than that seems to be the > optimum thing to do. > > On Tue, Feb 28, 2017 at 1:39 PM, Margaryan, Naira < > naira.margaryan at hsc.wvu.edu> wrote: > > > Absolutely agreed! > > > > Naira > > > > -----Original Message----- > > From: Frazier, John [mailto:john.frazier at roche.com] > > Sent: Tuesday, February 28, 2017 2:31 PM > > To: Jay Lundgren > > Cc: Margaryan, Naira ; Gareth Davis < > > garethdavisyuma at gmail.com>; histonet at lists.utsouthwestern.edu; > Margaryan, > > Naira > > Subject: Re: [Histonet] Recycled reagents in VIP processor > > > > As a six sigma consultant to histology laboratories, it has been my > > experience that recycling xylene and alcohol overall is not a cost saver. > > When you factor in both capital dollars and operational dollars, the > > savings is neutral. In addition to the neutral cost in recycling, you > have > > to concern yourself with the quality of your in product on a daily basis. > > The last piece in the equation is the handling of Xylene with multiple > > touch points in between. A movement within the histology world has begun > > with handling xylene (hazard > > waste) as little as possible and/or reducing or eliminating its use where > > possible. > > > > Sent from my iPhone > > > > > On Feb 27, 2017, at 6:25 PM, Jay Lundgren > wrote: > > > > > > When people say they are saving money by recycling reagents I always > > > wonder if they are including tech time (to run the still) in their > > calculations. > > > > > > Sincerely, > > > > > > Jay A. Lundgren, M.S., HTL (ASCP) > > > > > > On Wed, Feb 22, 2017 at 2:32 PM, Margaryan, Naira via Histonet < > > > histonet at lists.utsouthwestern.edu> wrote: > > > > > >> WOULD LIKE TO KNOW AS WELL - IT IS WASTE OF MONEY.... > > >> > > >> Thanks, > > >> Naira > > >> > > >> > > >> Ranked nationally in all 10 pediatric specialties by U.S. News & > > >> World Report (LCHOC Ver 1.0) > > >> > > >> > > >> This message contains confidential information and is intended only > > >> for the individual named. If you are not the named addressee you > > >> should not disseminate, distribute or copy this e-mail. Please notify > > >> the sender immediately by e-mail if you have received this e-mail by > > >> mistake and delete this e-mail from your system. E-mail transmission > > >> cannot be guaranteed to be secure or error-free as information could > > >> be intercepted, corrupted, lost, destroyed, arrive late or incomplete, > > or contain viruses. > > >> The sender therefore does not accept liability for any errors or > > >> omissions in the contents of this message, which arise as a result of > > >> e-mail transmission. If verification is required please request a > > >> hard-copy version. (LCHOC VER 1.0) > > >> > > >> ________________________________________ > > >> From: Gareth Davis via Histonet [histonet at lists.utsouthwestern.edu] > > >> Sent: Wednesday, February 22, 2017 2:23 PM > > >> To: histonet at lists.utsouthwestern.edu > > >> Subject: [Histonet] Recycled reagents in VIP processor > > >> > > >> Hi, > > >> I was always told not to use recycled reagents, i.e. Alcohol and > > >> Xylene, in processors. I am using a VIP 300, refurbished, and I > > >> would rather not use recycled reagents in it. But, during the last > > >> CAP inspection they suggested I use the recycled to save money. And > > >> now my administration wants to cut cost. Just wanted to know what > labs > > were doing. > > >> Thanks, > > >> > > >> > > >> -- > > >> Ms. Gareth B. Davis, B.S., HT, QIHC (ASCP)cm Yuma Gastroenterology > > >> Yuma, AZ 85364 > > >> 928-248-5259 > > >> _______________________________________________ > > >> Histonet mailing list > > >> Histonet at lists.utsouthwestern.edu > > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >> _______________________________________________ > > >> Histonet mailing list > > >> Histonet at lists.utsouthwestern.edu > > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >> > > > > > > > > > -- > Ms. Gareth B. Davis, B.S., HT, QIHC (ASCP)cm > Yuma Gastroenterology > Yuma, AZ 85364 > 928-248-5259 > > > > ---------- Forwarded message ---------- > From: Joanne Clark > To: "histonet at lists.utsouthwestern.edu" > > Cc: > Bcc: > Date: Tue, 28 Feb 2017 21:37:10 +0000 > Subject: Re: [Histonet] Histonet Digest, Vol 159, Issue 24 > > > > Hi Allison, I talked to my safety guy and there isn't anything specific. > They are telling you that because there is a fire hazard warning on > formalin cubes, but we know that the change of it catching fire is slim to > nil. They want you to use the same standards as with alcohol and xylene (1 > gallon per 100 square feet). It is all so dependent on who you have > inspecting you and what their background is. > > Joanne Clark, HT > Pathology Consultants of New Mexico > > ---------------------------------------------------------------------- > > Message: 1 > Date: Mon, 27 Feb 2017 19:26:54 +0000 > From: "Eck, Allison" > To: "'histonet at lists.utsouthwestern.edu'" > > Subject: [Histonet] square footage for formalin > Message-ID: > <4ED8C96A8F20FC4F883A92E2A0A0D64A97341B84 at DH-MAIL01.dhorg.org> > Content-Type: text/plain; charset="us-ascii" > > Good afternoon, > We have inspectors here and they are questioning the size of the room in > the operating room where they keep their 5 gallon cube. Does anyone know > of any square footage requirements for a room that where formalin is kept > and used? > > Thank you in advance > Allison > > Allison Eck, HTL(ASCP)cm,QLS, AHI(AMT) > Lead Tech Histology > Doylestown Hospital > 595 W State St > Doylestown, PA 18901 > 215-345-2264 > aeck at dh.org > > > > > ------------------------------ > > > Disclaimer: This electronic message may contain information that is > Proprietary, Confidential, or legally privileged or protected. It is > intended only for the use of the individual(s) and entity named in the > message. If you are not an intended recipient of this message, please > notify the sender immediately and delete the material from your computer. > Do not deliver, distribute or copy this message and do not disclose its > contents or take any action in reliance on the information it contains. > > > > > ---------- Forwarded message ---------- > From: Eileen Akemi Allison > To: Histonet > Cc: > Bcc: > Date: Wed, 1 Mar 2017 05:23:16 -0800 > Subject: [Histonet] Cassette Printers > Good morning Histoland! > > Our department is looking into purchasing a new cassette printer which has > the capability of interfacing with our AP Easy LIS System. I am currently > demoing a General Data ID/Positive CL-01 Laser Cassette Printer and label > printer system. We are a fairly small lab with limited space so a small > foot print is essential. We also do not need a multiple magazine. > > I would love to hear your comments, good, bad or indifferent? > > Thanks in advance > > Akemi Allison BS, HT/HTL (ASCP) > Pathology Manager > Monterey Bay GI Consultants Laboratory > 23 Upper Ragsdale Drive, Suite 200 > Monterey, CA 93940 > W: Email: aallison at montereygi.com > H: Email: akemiat3377 at gmail.com > > > > > ---------- Forwarded message ---------- > From: Eileen Akemi Allison > To: Histonet > Cc: > Bcc: > Date: Wed, 1 Mar 2017 05:30:35 -0800 > Subject: [Histonet] cassette printer additional request > Hi Again: > > I forgot to mention I am open to investigate other Cassette Printers other > than the General Data Printer? AlOf course, price, and dependability is a > big factor. Would love to hear your comments, good, bad or indifferent... > > Thanks in again! > > Akemi Allison BS, HT/HTL (ASCP) > Pathology Manager > Monterey Bay GI Consultants Laboratory > 23 Upper Ragsdale Drive, Suite 200 > Monterey, CA 93940 > W: Email: aallison at montereygi.com > H: Email: akemiat3377 at gmail.com > > > > > ---------- Forwarded message ---------- > From: "Blazek, Linda" > To: Eileen Akemi Allison , " > histonet at lists.utsouthwestern.edu" > Cc: > Bcc: > Date: Wed, 1 Mar 2017 09:50:13 -0500 > Subject: Re: [Histonet] cassette printer additional request > > We have the Primera Signature Cassette printer and like it a lot. Support > is excellent but we haven't needed it much. Set up is easy. Printing is > very good. We also have their slide printer that is interfaced with our > LIS. It's sold by Creative Waste Solutions. > http://cwsincorp.com/ > If you'd like any questions answered feel free to contact me. > > Linda Blazek HT (ASCP) > Pathology Lab Manager > GI Pathology of Dayton > Digestive Specialists, Inc > Phone: (937) 396-2623 > Email: lblazek at digestivespecialists.com > > > -----Original Message----- > From: Eileen Akemi Allison via Histonet [mailto:histonet at lists. > utsouthwestern.edu] > Sent: Wednesday, March 01, 2017 8:31 AM > To: Histonet > Subject: [Histonet] cassette printer additional request > > Hi Again: > > I forgot to mention I am open to investigate other Cassette Printers other > than the General Data Printer? AlOf course, price, and dependability is a > big factor. Would love to hear your comments, good, bad or indifferent... > > Thanks in again! > > Akemi Allison BS, HT/HTL (ASCP) > Pathology Manager > Monterey Bay GI Consultants Laboratory > 23 Upper Ragsdale Drive, Suite 200 > Monterey, CA 93940 > W: Email: aallison at montereygi.com > H: Email: akemiat3377 at gmail.com > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- John S. Frazier, MT(ASCP), MBA, LSSBB Senior Manager, RMS Consulting Roche Diagnostics Corporation Mobile: 1-520-305-5030 Fax: 1-520-229-6819 john.frazier at roche.com _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wendy at cwsincorp.com Wed Mar 1 13:26:32 2017 From: wendy at cwsincorp.com (Wendy) Date: Wed, 1 Mar 2017 19:26:32 +0000 Subject: [Histonet] Histonet Digest, Vol 160, Issue 1 In-Reply-To: References: Message-ID: <8BE14B3077AE9A449557AC6019C8D31B398104BA@SERVER.cw.local> Perhaps the out of pocket expense of a still for distilling your reagents is too expensive and too time consuming for some labs. There is an alternative using Gravity fed filtration through a carbon filter to filter out organics and impurities in alcohol and formalin. Distillation is not the only way to go. But I think the key is you have to have the desire to recycle and the do your own assessment as to whether or not it works for your lab. -----Original Message----- From: Frazier, John via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, March 01, 2017 10:43 AM To: Histonet (histonet at lists.utsouthwestern.edu) Subject: Re: [Histonet] Histonet Digest, Vol 160, Issue 1 One last statement about recycling Xylene and Alcohol and I do not have a dog in the fight. *Many labs I visit have recyclers and many of those do not use them any more due to poor or inconsistent quality of the end product and overall disillusionment of the money or time they thought they would save.* I am not telling labs not to recycle but if they ask me, I tell them what I have seen and give them some of the data around the process measurements we have collected. Some variables that are critical in gauging the value-add or non value-add are: - Cost of Waste disposal per gallon - Fully Burdened Salary of the lab individual/s that currently handle reagent changes (H&E stainer, Tissue Processor) vs. that of lab individual/s that will be or are handling the the recyclable reagents - Time to retrieve fresh reagents vs. time to move the recyclable reagent to and from the recycler. - Cost of PPE, i.e. respirator mask, gloves - Cost per square foot of Storage space for Hazardous waste vs. recycler foot print. This is an opportunity cost that recycling may provide for other types of storage. - Time to make dilutions of alcohol (100% to 95%, 85%,etc.) - Time to measure the concentrations of the recycled alcohol - Time for corrections if the concentrations is too low or too high - Is there a xylene substitute or instrument that does not require xylene and or alcohol - Others Your have to be objective about the process measurements of making the decision. What am I gaining vs. what am getting vs. what am I giving up. On Wed, Mar 1, 2017 at 1:00 PM, wrote: > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > Today's Topics: > > 1. Re: Recycled reagents in VIP processor (Frazier, John) > 2. Leica CM1950 (Clements, Mary Ann) > 3. Re: Recycled reagents in VIP processor (Margaryan, Naira) > 4. Re: Recycled reagents in VIP processor (Gareth Davis) > 5. Re: Histonet Digest, Vol 159, Issue 24 (Joanne Clark) > 6. Cassette Printers (Eileen Akemi Allison) > 7. cassette printer additional request (Eileen Akemi Allison) > 8. Re: cassette printer additional request (Blazek, Linda) > > > ---------- Forwarded message ---------- > From: "Frazier, John" > To: Jay Lundgren > Cc: "Margaryan, Naira" , Gareth Davis < > garethdavisyuma at gmail.com>, "histonet at lists.utsouthwestern.edu" < > histonet at lists.utsouthwestern.edu>, "naira.margaryan at hsc.wvu.edu" < > naira.margaryan at hsc.wvu.edu> > Bcc: > Date: Tue, 28 Feb 2017 14:30:46 -0500 > Subject: Re: [Histonet] Recycled reagents in VIP processor As a six > sigma consultant to histology laboratories, it has been my experience > that recycling xylene and alcohol overall is not a cost saver. When > you factor in both capital dollars and operational dollars, the > savings is neutral. In addition to the neutral cost in recycling, you > have to concern yourself with the quality of your in product on a > daily basis. The last piece in the equation is the handling of Xylene > with multiple touch points in between. A movement within the > histology world has begun with handling xylene (hazard > waste) as little as possible and/or reducing or eliminating its use > where possible. > > Sent from my iPhone > > > On Feb 27, 2017, at 6:25 PM, Jay Lundgren wrote: > > > > When people say they are saving money by recycling reagents I always > wonder > > if they are including tech time (to run the still) in their calculations. > > > > Sincerely, > > > > Jay A. Lundgren, M.S., HTL > > (ASCP) > > > > On Wed, Feb 22, 2017 at 2:32 PM, Margaryan, Naira via Histonet < > > histonet at lists.utsouthwestern.edu> wrote: > > > >> WOULD LIKE TO KNOW AS WELL - IT IS WASTE OF MONEY.... > >> > >> Thanks, > >> Naira > >> > >> > >> Ranked nationally in all 10 pediatric specialties by U.S. News & > >> World Report (LCHOC Ver 1.0) > >> > >> > >> This message contains confidential information and is intended only > >> for the individual named. If you are not the named addressee you > >> should not disseminate, distribute or copy this e-mail. Please > >> notify the sender immediately by e-mail if you have received this > >> e-mail by mistake and delete this e-mail from your system. E-mail > >> transmission cannot be guaranteed to be secure or error-free as > >> information could be > intercepted, > >> corrupted, lost, destroyed, arrive late or incomplete, or contain > viruses. > >> The sender therefore does not accept liability for any errors or > omissions > >> in the contents of this message, which arise as a result of e-mail > >> transmission. If verification is required please request a > >> hard-copy version. (LCHOC VER 1.0) > >> > >> ________________________________________ > >> From: Gareth Davis via Histonet [histonet at lists.utsouthwestern.edu] > >> Sent: Wednesday, February 22, 2017 2:23 PM > >> To: histonet at lists.utsouthwestern.edu > >> Subject: [Histonet] Recycled reagents in VIP processor > >> > >> Hi, > >> I was always told not to use recycled reagents, i.e. Alcohol and > Xylene, in > >> processors. I am using a VIP 300, refurbished, and I would rather > >> not > use > >> recycled reagents in it. But, during the last CAP inspection they > >> suggested I use the recycled to save money. And now my > >> administration wants to cut cost. Just wanted to know what labs were doing. > >> Thanks, > >> > >> > >> -- > >> Ms. Gareth B. Davis, B.S., HT, QIHC (ASCP)cm Yuma Gastroenterology > >> Yuma, AZ 85364 > >> 928-248-5259 > >> _______________________________________________ > >> Histonet mailing list > >> Histonet at lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> _______________________________________________ > >> Histonet mailing list > >> Histonet at lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> > > > > > > > ---------- Forwarded message ---------- > From: "Clements, Mary Ann" > To: "'histonet at lists.utsouthwestern.edu'" < > histonet at lists.utsouthwestern.edu> > Cc: > Bcc: > Date: Tue, 28 Feb 2017 19:50:49 +0000 > Subject: [Histonet] Leica CM1950 > We are interested in purchasing the Leica CM 1950 cryostat. Would love to > hear your comments, good, bad or indifferent... > Thanks in advance! > > Mary Ann Clements, B.S. > Biorepository Manager > Department of Pathology & Anatomy > Eastern Virginia Medical School > 700 West Olney Road > Lewis Hall, Room 3011 > Norfolk, VA 23507 > > email: clemenma at evms.edu > phone: (757) 446-7910 > fax: (757) 446-7059 > > > > > ---------- Forwarded message ---------- > From: "Margaryan, Naira" > To: "Frazier, John" , Jay Lundgren < > jaylundgren at gmail.com> > Cc: "Margaryan, Naira" , Gareth Davis < > garethdavisyuma at gmail.com>, "histonet at lists.utsouthwestern.edu" < > histonet at lists.utsouthwestern.edu> > Bcc: > Date: Tue, 28 Feb 2017 20:39:10 +0000 > Subject: Re: [Histonet] Recycled reagents in VIP processor > Absolutely agreed! > > Naira > > -----Original Message----- > From: Frazier, John [mailto:john.frazier at roche.com] > Sent: Tuesday, February 28, 2017 2:31 PM > To: Jay Lundgren > Cc: Margaryan, Naira ; Gareth Davis < > garethdavisyuma at gmail.com>; histonet at lists.utsouthwestern.edu; Margaryan, > Naira > Subject: Re: [Histonet] Recycled reagents in VIP processor > > As a six sigma consultant to histology laboratories, it has been my > experience that recycling xylene and alcohol overall is not a cost saver. > When you factor in both capital dollars and operational dollars, the > savings is neutral. In addition to the neutral cost in recycling, you have > to concern yourself with the quality of your in product on a daily basis. > The last piece in the equation is the handling of Xylene with multiple > touch points in between. A movement within the histology world has begun > with handling xylene (hazard > waste) as little as possible and/or reducing or eliminating its use where > possible. > > Sent from my iPhone > > > On Feb 27, 2017, at 6:25 PM, Jay Lundgren wrote: > > > > When people say they are saving money by recycling reagents I always > > wonder if they are including tech time (to run the still) in their > calculations. > > > > Sincerely, > > > > Jay A. Lundgren, M.S., HTL (ASCP) > > > > On Wed, Feb 22, 2017 at 2:32 PM, Margaryan, Naira via Histonet < > > histonet at lists.utsouthwestern.edu> wrote: > > > >> WOULD LIKE TO KNOW AS WELL - IT IS WASTE OF MONEY.... > >> > >> Thanks, > >> Naira > >> > >> > >> Ranked nationally in all 10 pediatric specialties by U.S. News & > >> World Report (LCHOC Ver 1.0) > >> > >> > >> This message contains confidential information and is intended only > >> for the individual named. If you are not the named addressee you > >> should not disseminate, distribute or copy this e-mail. Please notify > >> the sender immediately by e-mail if you have received this e-mail by > >> mistake and delete this e-mail from your system. E-mail transmission > >> cannot be guaranteed to be secure or error-free as information could > >> be intercepted, corrupted, lost, destroyed, arrive late or incomplete, > or contain viruses. > >> The sender therefore does not accept liability for any errors or > >> omissions in the contents of this message, which arise as a result of > >> e-mail transmission. If verification is required please request a > >> hard-copy version. (LCHOC VER 1.0) > >> > >> ________________________________________ > >> From: Gareth Davis via Histonet [histonet at lists.utsouthwestern.edu] > >> Sent: Wednesday, February 22, 2017 2:23 PM > >> To: histonet at lists.utsouthwestern.edu > >> Subject: [Histonet] Recycled reagents in VIP processor > >> > >> Hi, > >> I was always told not to use recycled reagents, i.e. Alcohol and > >> Xylene, in processors. I am using a VIP 300, refurbished, and I > >> would rather not use recycled reagents in it. But, during the last > >> CAP inspection they suggested I use the recycled to save money. And > >> now my administration wants to cut cost. Just wanted to know what labs > were doing. > >> Thanks, > >> > >> > >> -- > >> Ms. Gareth B. Davis, B.S., HT, QIHC (ASCP)cm Yuma Gastroenterology > >> Yuma, AZ 85364 > >> 928-248-5259 > >> _______________________________________________ > >> Histonet mailing list > >> Histonet at lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> _______________________________________________ > >> Histonet mailing list > >> Histonet at lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> > > > > > ---------- Forwarded message ---------- > From: Gareth Davis > To: "Margaryan, Naira" > Cc: "Frazier, John" , Jay Lundgren < > jaylundgren at gmail.com>, "Margaryan, Naira" , > "histonet at lists.utsouthwestern.edu" > Bcc: > Date: Tue, 28 Feb 2017 13:57:24 -0700 > Subject: Re: [Histonet] Recycled reagents in VIP processor > When using Xylene in a lab, it's my opinion that the savings is associated > with the size of the lab. I am in a very small lab, where I am the only > Histotech. I recycle both alcohol and Xylene and monitor both closely. > For me, it would cost effective to recycle and use as much as possible, > instead of purchasing fresh. But, I agree, if it's a larger lab it may not > be as cost effective. Also, if the lab is in the position to order a > substitute for Xylene, where it would be cheaper, than that seems to be the > optimum thing to do. > > On Tue, Feb 28, 2017 at 1:39 PM, Margaryan, Naira < > naira.margaryan at hsc.wvu.edu> wrote: > > > Absolutely agreed! > > > > Naira > > > > -----Original Message----- > > From: Frazier, John [mailto:john.frazier at roche.com] > > Sent: Tuesday, February 28, 2017 2:31 PM > > To: Jay Lundgren > > Cc: Margaryan, Naira ; Gareth Davis < > > garethdavisyuma at gmail.com>; histonet at lists.utsouthwestern.edu; > Margaryan, > > Naira > > Subject: Re: [Histonet] Recycled reagents in VIP processor > > > > As a six sigma consultant to histology laboratories, it has been my > > experience that recycling xylene and alcohol overall is not a cost saver. > > When you factor in both capital dollars and operational dollars, the > > savings is neutral. In addition to the neutral cost in recycling, you > have > > to concern yourself with the quality of your in product on a daily basis. > > The last piece in the equation is the handling of Xylene with multiple > > touch points in between. A movement within the histology world has begun > > with handling xylene (hazard > > waste) as little as possible and/or reducing or eliminating its use where > > possible. > > > > Sent from my iPhone > > > > > On Feb 27, 2017, at 6:25 PM, Jay Lundgren > wrote: > > > > > > When people say they are saving money by recycling reagents I always > > > wonder if they are including tech time (to run the still) in their > > calculations. > > > > > > Sincerely, > > > > > > Jay A. Lundgren, M.S., HTL (ASCP) > > > > > > On Wed, Feb 22, 2017 at 2:32 PM, Margaryan, Naira via Histonet < > > > histonet at lists.utsouthwestern.edu> wrote: > > > > > >> WOULD LIKE TO KNOW AS WELL - IT IS WASTE OF MONEY.... > > >> > > >> Thanks, > > >> Naira > > >> > > >> > > >> Ranked nationally in all 10 pediatric specialties by U.S. News & > > >> World Report (LCHOC Ver 1.0) > > >> > > >> > > >> This message contains confidential information and is intended only > > >> for the individual named. If you are not the named addressee you > > >> should not disseminate, distribute or copy this e-mail. Please notify > > >> the sender immediately by e-mail if you have received this e-mail by > > >> mistake and delete this e-mail from your system. E-mail transmission > > >> cannot be guaranteed to be secure or error-free as information could > > >> be intercepted, corrupted, lost, destroyed, arrive late or incomplete, > > or contain viruses. > > >> The sender therefore does not accept liability for any errors or > > >> omissions in the contents of this message, which arise as a result of > > >> e-mail transmission. If verification is required please request a > > >> hard-copy version. (LCHOC VER 1.0) > > >> > > >> ________________________________________ > > >> From: Gareth Davis via Histonet [histonet at lists.utsouthwestern.edu] > > >> Sent: Wednesday, February 22, 2017 2:23 PM > > >> To: histonet at lists.utsouthwestern.edu > > >> Subject: [Histonet] Recycled reagents in VIP processor > > >> > > >> Hi, > > >> I was always told not to use recycled reagents, i.e. Alcohol and > > >> Xylene, in processors. I am using a VIP 300, refurbished, and I > > >> would rather not use recycled reagents in it. But, during the last > > >> CAP inspection they suggested I use the recycled to save money. And > > >> now my administration wants to cut cost. Just wanted to know what > labs > > were doing. > > >> Thanks, > > >> > > >> > > >> -- > > >> Ms. Gareth B. Davis, B.S., HT, QIHC (ASCP)cm Yuma Gastroenterology > > >> Yuma, AZ 85364 > > >> 928-248-5259 > > >> _______________________________________________ > > >> Histonet mailing list > > >> Histonet at lists.utsouthwestern.edu > > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >> _______________________________________________ > > >> Histonet mailing list > > >> Histonet at lists.utsouthwestern.edu > > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >> > > > > > > > > > -- > Ms. Gareth B. Davis, B.S., HT, QIHC (ASCP)cm > Yuma Gastroenterology > Yuma, AZ 85364 > 928-248-5259 > > > > ---------- Forwarded message ---------- > From: Joanne Clark > To: "histonet at lists.utsouthwestern.edu" > > Cc: > Bcc: > Date: Tue, 28 Feb 2017 21:37:10 +0000 > Subject: Re: [Histonet] Histonet Digest, Vol 159, Issue 24 > > > > Hi Allison, I talked to my safety guy and there isn't anything specific. > They are telling you that because there is a fire hazard warning on > formalin cubes, but we know that the change of it catching fire is slim to > nil. They want you to use the same standards as with alcohol and xylene (1 > gallon per 100 square feet). It is all so dependent on who you have > inspecting you and what their background is. > > Joanne Clark, HT > Pathology Consultants of New Mexico > > ---------------------------------------------------------------------- > > Message: 1 > Date: Mon, 27 Feb 2017 19:26:54 +0000 > From: "Eck, Allison" > To: "'histonet at lists.utsouthwestern.edu'" > > Subject: [Histonet] square footage for formalin > Message-ID: > <4ED8C96A8F20FC4F883A92E2A0A0D64A97341B84 at DH-MAIL01.dhorg.org> > Content-Type: text/plain; charset="us-ascii" > > Good afternoon, > We have inspectors here and they are questioning the size of the room in > the operating room where they keep their 5 gallon cube. Does anyone know > of any square footage requirements for a room that where formalin is kept > and used? > > Thank you in advance > Allison > > Allison Eck, HTL(ASCP)cm,QLS, AHI(AMT) > Lead Tech Histology > Doylestown Hospital > 595 W State St > Doylestown, PA 18901 > 215-345-2264 > aeck at dh.org > > > > > ------------------------------ > > > Disclaimer: This electronic message may contain information that is > Proprietary, Confidential, or legally privileged or protected. It is > intended only for the use of the individual(s) and entity named in the > message. If you are not an intended recipient of this message, please > notify the sender immediately and delete the material from your computer. > Do not deliver, distribute or copy this message and do not disclose its > contents or take any action in reliance on the information it contains. > > > > > ---------- Forwarded message ---------- > From: Eileen Akemi Allison > To: Histonet > Cc: > Bcc: > Date: Wed, 1 Mar 2017 05:23:16 -0800 > Subject: [Histonet] Cassette Printers > Good morning Histoland! > > Our department is looking into purchasing a new cassette printer which has > the capability of interfacing with our AP Easy LIS System. I am currently > demoing a General Data ID/Positive CL-01 Laser Cassette Printer and label > printer system. We are a fairly small lab with limited space so a small > foot print is essential. We also do not need a multiple magazine. > > I would love to hear your comments, good, bad or indifferent? > > Thanks in advance > > Akemi Allison BS, HT/HTL (ASCP) > Pathology Manager > Monterey Bay GI Consultants Laboratory > 23 Upper Ragsdale Drive, Suite 200 > Monterey, CA 93940 > W: Email: aallison at montereygi.com > H: Email: akemiat3377 at gmail.com > > > > > ---------- Forwarded message ---------- > From: Eileen Akemi Allison > To: Histonet > Cc: > Bcc: > Date: Wed, 1 Mar 2017 05:30:35 -0800 > Subject: [Histonet] cassette printer additional request > Hi Again: > > I forgot to mention I am open to investigate other Cassette Printers other > than the General Data Printer? AlOf course, price, and dependability is a > big factor. Would love to hear your comments, good, bad or indifferent... > > Thanks in again! > > Akemi Allison BS, HT/HTL (ASCP) > Pathology Manager > Monterey Bay GI Consultants Laboratory > 23 Upper Ragsdale Drive, Suite 200 > Monterey, CA 93940 > W: Email: aallison at montereygi.com > H: Email: akemiat3377 at gmail.com > > > > > ---------- Forwarded message ---------- > From: "Blazek, Linda" > To: Eileen Akemi Allison , " > histonet at lists.utsouthwestern.edu" > Cc: > Bcc: > Date: Wed, 1 Mar 2017 09:50:13 -0500 > Subject: Re: [Histonet] cassette printer additional request > > We have the Primera Signature Cassette printer and like it a lot. Support > is excellent but we haven't needed it much. Set up is easy. Printing is > very good. We also have their slide printer that is interfaced with our > LIS. It's sold by Creative Waste Solutions. > http://cwsincorp.com/ > If you'd like any questions answered feel free to contact me. > > Linda Blazek HT (ASCP) > Pathology Lab Manager > GI Pathology of Dayton > Digestive Specialists, Inc > Phone: (937) 396-2623 > Email: lblazek at digestivespecialists.com > > > -----Original Message----- > From: Eileen Akemi Allison via Histonet [mailto:histonet at lists. > utsouthwestern.edu] > Sent: Wednesday, March 01, 2017 8:31 AM > To: Histonet > Subject: [Histonet] cassette printer additional request > > Hi Again: > > I forgot to mention I am open to investigate other Cassette Printers other > than the General Data Printer? AlOf course, price, and dependability is a > big factor. Would love to hear your comments, good, bad or indifferent... > > Thanks in again! > > Akemi Allison BS, HT/HTL (ASCP) > Pathology Manager > Monterey Bay GI Consultants Laboratory > 23 Upper Ragsdale Drive, Suite 200 > Monterey, CA 93940 > W: Email: aallison at montereygi.com > H: Email: akemiat3377 at gmail.com > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- John S. Frazier, MT(ASCP), MBA, LSSBB Senior Manager, RMS Consulting Roche Diagnostics Corporation Mobile: 1-520-305-5030 Fax: 1-520-229-6819 john.frazier at roche.com _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LRaff at uropartners.com Thu Mar 2 10:37:12 2017 From: LRaff at uropartners.com (Lester Raff MD) Date: Thu, 2 Mar 2017 16:37:12 +0000 Subject: [Histonet] New Lab Blog Posting Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF11280E89@COLOEXCH01.uropartners.local> I hope all are doing well. A return look at prostate cancer in the lab. http://www.chicagonow.com/downsize-maybe/2017/03/prostate-cancer-peaking-behind-the-pathologist-screen/ Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From relia1 at earthlink.net Thu Mar 2 11:35:57 2017 From: relia1 at earthlink.net (Pam Barker) Date: Thu, 2 Mar 2017 12:35:57 -0500 Subject: [Histonet] Exciting Opportunities in California!! Histotechs, Pathologists Assistants, Managers!!! Message-ID: <00d701d2937b$7374a350$5a5de9f0$@earthlink.net> Hi Histonetters!! I hope everybody is having a great day!! Things are really hopping in California I have some exciting opportunities in a couple of different areas. Here's what's going on: Management: San Diego Orange County HT/HTL San Diego Orange County Modesto PA San Diego All of these are full time permanent positions. My clients offer competitive pay, excellent benefits and a team that can't wait to welcome you aboard. If you are interested please contact me asap. You can reach me by cell/text at 407-353-5070 or toll free at the office at 866-607-3542 or e-mail me at relia1 at earthlink.net I'm NOT like those OTHER recruiters I won't share your resume with anyone before telling you who the client is. Your resume and your job search are confidential with me!!! YOU and YOUR career are my priority!!! Have a great day!!! Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From relia1 at earthlink.net Thu Mar 2 11:47:51 2017 From: relia1 at earthlink.net (Pam Barker) Date: Thu, 2 Mar 2017 12:47:51 -0500 Subject: [Histonet] RELIA Hot Histology Job Alert!! Exciting Opportunities in California!! Histotechs, Pathologists Assistants, Managers!!! Message-ID: <00e401d2937d$1d4a2840$57de78c0$@earthlink.net> Hi Histonetters!! I hope everybody is having a great day!! Things are really hopping in California I have some exciting opportunities in a couple of different areas. Here's what's going on: Management: San Diego Orange County HT/HTL San Diego Orange County Modesto PA San Diego All of these are full time permanent positions. My clients offer competitive pay, excellent benefits and a team that can't wait to welcome you aboard. If you are interested please contact me asap. You can reach me by cell/text at 407-353-5070 or toll free at the office at 866-607-3542 or e-mail me at relia1 at earthlink.net I'm NOT like those OTHER recruiters I won't share your resume with anyone before telling you who the client is. Your resume and your job search are confidential with me!!! YOU and YOUR career are my priority!!! Have a great day!!! Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From relia1 at earthlink.net Fri Mar 3 07:58:26 2017 From: relia1 at earthlink.net (Pam Barker) Date: Fri, 3 Mar 2017 08:58:26 -0500 Subject: [Histonet] RELIA Hot Histology Job Alert!! Live and work in Beautiful Coastal North Carolina with this RELIA exclusive!!! Message-ID: <006001d29426$43e93230$cbbb9690$@earthlink.net> Hi Histonetters!! TGIF!!! And it's almost Spring Break!! Wouldn't you love to live in flip flops and bermuda shorts? If yes CALL ME!! I have an amazing opportunity in Coastal NC. This is a full time permanent position and my client is looking for an ASCP certified histotech that can perform routine histology. Competitive pay, excellent benefits and a team that can't wait to welcome you aboard. If you are interested call /text me right away on my cell at 407-353-5070. I will even stop in the middle of my Zumba class to respond to you and I LOVE MY ZUMBA CLASS!! You can also reach me toll free at the office at 866-607-3542 or via email at relia1 at earthlink.net If you know someone who might be interested and you refer them to me you will earn a referral fee if I place them. That means if they get placed in this job or in another position a year from now. I never forget a referral. Have a great day and a great weekend!! Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From Christine.Altemus at smha.org Fri Mar 3 09:03:38 2017 From: Christine.Altemus at smha.org (Altemus, Christine) Date: Fri, 3 Mar 2017 15:03:38 +0000 Subject: [Histonet] Looking for control tissue Message-ID: <34E426C83C2EC346806B6E8CCDA6D26955577D49@TX1P03DAG0309.apptixhealth.net> Hi - We are currently in need of Lung Adenocarcinoma and Lung Squamous Cell carcinoma tissue to use for controls for Pulmo Panel IHC Staining. Stains include Desmoglein 3 + Napsin A cocktail, TTF-1 + CK5 cocktail and p63+TRIM29 Cocktail. If anyone has some that they can spare, kindly pm me. Thanks in advance for any assistance you can provide. Chrissy - St. Mary's Healthcare, Amsterdam-NY CONFIDENTIALITY NOTICE: This email message and any accompanying data or files is confidential and may contain privileged information intended only for the named recipient(s). If you are not the intended recipient(s), you are hereby notified that the dissemination, distribution, and or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at the email address above, delete this email from your computer, and destroy any copies in any form immediately. Receipt by anyone other than the named recipient(s) is not a waiver of any attorney-client, work product, or other applicable privilege. From INAIR at coh.org Fri Mar 3 12:25:59 2017 From: INAIR at coh.org (Nair, Indu) Date: Fri, 3 Mar 2017 18:25:59 +0000 Subject: [Histonet] query Message-ID: Is there a protocol to section un-decalcified bone? Or is there a laboratory that does this routinely? thank you indu --------------------------------------------------------------------- -SECURITY/CONFIDENTIALITY WARNING- This message (and any attachments) are intended solely for the individual or entity to which they are addressed. This communication may contain information that is privileged, confidential, or exempt from disclosure under applicable law (e.g., personal health information, research data, financial information). Because this e-mail has been sent without encryption, individuals other than the intended recipient may be able to view the information, forward it to others or tamper with the information without the knowledge or consent of the sender. If you are not the intended recipient, or the employee or person responsible for delivering the message to the intended recipient, any dissemination, distribution or copying of the communication is strictly prohibited. If you received the communication in error, please notify the sender immediately by replying to this message and deleting the message and any accompanying files from your system. If, due to the security risks, you do not wish to receive further communications via e-mail, please reply to this message and inform the sender that you do not wish to receive further e-mail from the sender. (LCP301) --------------------------------------------------------------------- From angie at ka-recruiting.com Fri Mar 3 12:28:49 2017 From: angie at ka-recruiting.com (Angie Laparidis) Date: Fri, 3 Mar 2017 13:28:49 -0500 Subject: [Histonet] Day Shift Histotech in Buffalo Message-ID: TGIF Histonet subscribers! I wanted to fill you all in on a brand new DAY SHIFT position in the Buffalo, NY area! This location is fantastic for those who value all four seasons! Day shift opportunities are hard to come by, and this position will not be open very long. Take a look at the area (http://www.visitbuffaloniagara.com/) and send over a resume so we can further discuss your next career move! Look forward to hearing from you! Sincerely, *Angie Laparidis*Healthcare Recruiter K.A. Recruiting, Inc. 10 Post Office Square, 8th Floor South, Boston, MA, 02109 W: 617.746-2744 (*please note this is a new number*) F: (617) 507-8009 Angie at ka-recruiting.com Our openings are updated daily at www.ka-recruiting.com From plucas at biopath.org Fri Mar 3 12:48:37 2017 From: plucas at biopath.org (Paula) Date: Fri, 3 Mar 2017 10:48:37 -0800 Subject: [Histonet] VIPE300 Message-ID: <001401d2944e$c644e710$52ceb530$@biopath.org> Hello, We're looking at purchasing a refurbished VIP E300 and I was hoping for some feedback on this processor. Is it reliable, dependable, good workmanship kind of machine or are they nothing but trouble? Thanks for any replies and have a good day. Paula From b-frederick at northwestern.edu Fri Mar 3 13:42:47 2017 From: b-frederick at northwestern.edu (Bernice Frederick) Date: Fri, 3 Mar 2017 19:42:47 +0000 Subject: [Histonet] TUNEL Message-ID: Hello all. Would there be any reason that slides stained with TUNEL (ISH) would not pick up the Mayers counterstain? We use it for IHC and it is fine. Mayers for 7 minutes and then ammonia water for 1 minute. We do that rather than rinse for 15 minutes and there are usually no issues. Could a reagent in the stain be causing it? DAPI is used... Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick at northwestern.edu From FMonson at wcupa.edu Fri Mar 3 14:09:55 2017 From: FMonson at wcupa.edu (Monson, Frederick) Date: Fri, 3 Mar 2017 20:09:55 +0000 Subject: [Histonet] query In-Reply-To: References: Message-ID: <9498aff695244c07968c794a78ff9329@WCUXCHP08.PASSHE.LCL> Have no expertise other with 'library.' So, here is some new stuff that I found. http://www.autoqbiosciences.com/products/3d-laser-nanodissection-systems/tissue-surgeon/ http://www.autoqbiosciences.com/_webedit/uploaded-files/All%20Files/Nano/LLS-Brosch%25C3%25BCre_TissueSurgeon_HardTissue_2014-03-18_web.pdf There are lots of methods, but few that can be followed with microdissection. Cheers, Fred Monson Frederick C. Monson, PhD Technical Director Center for Microanalysis and Imaging, Research and Training (CMIRT) West Chester University of PA Geology-Astronomy 750 South Church St. West Chester, PA, 19383 fmonson at wcupa.edu 610-738-0437(Work) -----Original Message----- From: Nair, Indu via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, March 03, 2017 1:26 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] query Is there a protocol to section un-decalcified bone? Or is there a laboratory that does this routinely? thank you indu --------------------------------------------------------------------- -SECURITY/CONFIDENTIALITY WARNING- This message (and any attachments) are intended solely for the individual or entity to which they are addressed. This communication may contain information that is privileged, confidential, or exempt from disclosure under applicable law (e.g., personal health information, research data, financial information). Because this e-mail has been sent without encryption, individuals other than the intended recipient may be able to view the information, forward it to others or tamper with the information without the knowledge or consent of the sender. If you are not the intended recipient, or the employee or person responsible for delivering the message to the intended recipient, any dissemination, distribution or copying of the communication is strictly prohibited. If you received the communication in error, please notify the sender immediately by replying to this message and deleting the message and any accompanying files from your system. If, due to the security risks, you do not w ish to receive further communications via e-mail, please reply to this message and inform the sender that you do not wish to receive further e-mail from the sender. (LCP301) --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mills at 3scan.com Fri Mar 3 14:16:17 2017 From: mills at 3scan.com (Caroline Miller) Date: Fri, 3 Mar 2017 12:16:17 -0800 Subject: [Histonet] TUNEL In-Reply-To: References: Message-ID: I have done that a lot and I don't see why it would not pick up hx or DAPI. Was there anything left on the slide? Maybe it was mounted in aqueous already?? mills On Fri, Mar 3, 2017 at 11:42 AM, Bernice Frederick via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hello all. > Would there be any reason that slides stained with TUNEL (ISH) would not > pick up the Mayers counterstain? We use it for IHC and it is fine. Mayers > for 7 minutes and then ammonia water for 1 minute. We do that rather than > rinse for 15 minutes and there are usually no issues. Could a reagent in > the stain be causing it? DAPI is used... > Bernice > > Bernice Frederick HTL (ASCP) > Senior Research Tech > Pathology Core Facility > Robert. H. Lurie Cancer Center > Northwestern University > 710 N Fairbanks Court > Olson 8-421 > Chicago,IL 60611 > 312-503-3723 > b-frederick at northwestern.edu > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 From vtolley25 at gmail.com Fri Mar 3 18:07:57 2017 From: vtolley25 at gmail.com (Val Tolley) Date: Fri, 3 Mar 2017 16:07:57 -0800 Subject: [Histonet] Label-less slides Message-ID: <4886E037-1456-4044-84D0-FC5B561A8E31@gmail.com> Hi All- Does anyone know where I can buy charged slides without a frosted end for a label? All I can find is plain, uncharged. ...Or alternatively, a charged slide with a shortened frosted edge allowing more room at the top of the slide for tissue adhesion. Val From lynettepav at gmail.com Sat Mar 4 13:40:15 2017 From: lynettepav at gmail.com (Lynette Pavelich) Date: Sat, 4 Mar 2017 14:40:15 -0500 Subject: [Histonet] IHC on alcoholic fixed cytology smears Message-ID: Hello, I will soon be starting the process of validating many cytoplasmic, membranous, and nuclear antibodies on alcoholic fixed cytology smears. They will be performed using the Bond III. In doing research on the subject, I am finding many different variables, and it is starting to be confusing. Is anyone willing to share their processes, or can suggest good reading material for IHC with alcohol fixed cytology smears? Hints, tips, tried and true methods would be greatly appreciated. Thank you, Lynette Pavelich, HT(ASCP) From vicki.kalscheur at wisc.edu Sat Mar 4 15:44:01 2017 From: vicki.kalscheur at wisc.edu (Vicki Kalscheur) Date: Sat, 04 Mar 2017 21:44:01 +0000 Subject: [Histonet] Indu, Check the histonet archives Message-ID: Feel free to contact me with questions on bone. Vicki L. Kalscheur School of Veterinary Medicine Comparative Orthopedic Research Laboratory 2015 Linden Drive Madison, WI 53706-1100 Phone: 608-262-8534 vicki.kalscheur at wisc.edu From rjbuesa at yahoo.com Sun Mar 5 09:45:41 2017 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Sun, 5 Mar 2017 15:45:41 +0000 (UTC) Subject: [Histonet] IHC on alcoholic fixed cytology smears In-Reply-To: References: Message-ID: <736160994.2210858.1488728741816@mail.yahoo.com> M best advise is contacting Dako and obtain from them the IHC manual, which covers essentially everything you need to know on this subject.Ren? On Saturday, March 4, 2017 2:51 PM, Lynette Pavelich via Histonet wrote: Hello, I will soon be starting the process of validating many cytoplasmic, membranous, and nuclear antibodies on alcoholic fixed cytology smears. They will be performed using the Bond III. In doing research on the subject, I am finding many different variables, and it is starting to be confusing. Is anyone willing to share their processes, or can suggest good reading material for IHC with alcohol fixed cytology smears? Hints, tips, tried and true methods would be greatly appreciated. Thank you, Lynette Pavelich, HT(ASCP) _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ASelf at tidelandshealth.org Mon Mar 6 09:28:12 2017 From: ASelf at tidelandshealth.org (Amy Self) Date: Mon, 6 Mar 2017 10:28:12 -0500 Subject: [Histonet] CPT code 88342 Message-ID: Good Morning Histonetters, For Meditech users who download 3M files for medical necessity, has anyone had any issues with CPT code 88342 rejecting and only accepting diagnosis codes specific to lung (dx code C34... ). Thanks in advance for your help, Amy Self Histology Lab Senior Tech Lab Tidelands Georgetown Memorial Hospital 606 Black River Road Georgetown, SC 29440 843-520-8711 ASelf at tidelandshealth.org NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. From BZIMMERM at augusta.edu Mon Mar 6 09:28:37 2017 From: BZIMMERM at augusta.edu (Zimmerman, Billie) Date: Mon, 6 Mar 2017 15:28:37 +0000 Subject: [Histonet] GSH Annual Histopalooza April 21 - 23 Legacy Lodge Lake Lanier Islands, Buford, Georgia Message-ID: The annual Histopalooza is just around the corner. The annual event is April 21 - 23 . Please visit our website for details: http://www.histosearch.com/gsh/ Reserve your room by March 21, which is only 15 days away. Registration for the event is $135 prior to April 10th. What a steal compared to other CEU opportunities out there. Talk to previous attendees for their opinion of Legacy Lodge. I assure you it's NOT the Bates motel. Billie Zimmerman GSH PR From relia1 at earthlink.net Mon Mar 6 10:01:08 2017 From: relia1 at earthlink.net (Pam Barker) Date: Mon, 6 Mar 2017 11:01:08 -0500 Subject: [Histonet] Histotechnology Professionals Day is March 10, 2017. Message-ID: <0ac501d29692$e89c6180$b9d52480$@earthlink.net> Dear Histonetters! This Friday March 10 is Histotechnology Professionals Day!! The National Society for Histotechnology has some great suggestions for ways to celebrate. Here is the link: http://www.nsh.org/content/histotechnology-professionals-day Happy Histotechnology Professionals Day! From RELIA! This year we mark 12 years serving the Histology community as the only recruiting firm working exclusively in nationwide permanent placement of histology professionals! We look forward to many more years together!! Thank you for your time, your business, your support and your fellowship! Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From phruntedeske at gmail.com Mon Mar 6 10:08:47 2017 From: phruntedeske at gmail.com (Keith Brown) Date: Mon, 6 Mar 2017 11:08:47 -0500 Subject: [Histonet] Newbe help Message-ID: Hi. I've looked through the archives of this group to little avail. If i missed an answer to my questions that was previously posted to this list, my apologies. I'm looking to get into the histotech field, but I'm not sure how to go about it. I'm changing careers after 20 years as a journalist. I have an associates and a bachelor's degree, neither of which are in anything remotely science-y: Liberal Arts and Journalism, respectively. I live in New Jersey, where there does not appear to be any histology training programs, yet most of the jobs I see posted throughout the state want a certification. I have a friend in Seattle who fell into histology after journalism, but he admits he got lucky and still does not have a certification. He gets paid slightly less, he says, but not by much. It sounds like a good career choice for me, but I'm not sure how to get it started. Any and all advice is appreciated. Thanks, Keith. From wbenton at cua.md Mon Mar 6 10:34:40 2017 From: wbenton at cua.md (Walter Benton) Date: Mon, 6 Mar 2017 16:34:40 +0000 Subject: [Histonet] Newbe help In-Reply-To: References: Message-ID: Start with this link and navigate through the website. There are various online and in-person programs. Best of luck. http://nsh.org/content/schools -----Original Message----- From: Keith Brown via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, March 06, 2017 11:09 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Newbe help Hi. I've looked through the archives of this group to little avail. If i missed an answer to my questions that was previously posted to this list, my apologies. I'm looking to get into the histotech field, but I'm not sure how to go about it. I'm changing careers after 20 years as a journalist. I have an associates and a bachelor's degree, neither of which are in anything remotely science-y: Liberal Arts and Journalism, respectively. I live in New Jersey, where there does not appear to be any histology training programs, yet most of the jobs I see posted throughout the state want a certification. I have a friend in Seattle who fell into histology after journalism, but he admits he got lucky and still does not have a certification. He gets paid slightly less, he says, but not by much. It sounds like a good career choice for me, but I'm not sure how to get it started. Any and all advice is appreciated. Thanks, Keith. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From alamberth at lji.org Mon Mar 6 11:57:33 2017 From: alamberth at lji.org (Angela Lamberth) Date: Mon, 6 Mar 2017 09:57:33 -0800 Subject: [Histonet] Metabisulfite rinse for PAS reaction (potassium vs sodium) Message-ID: In Carson?s 3rd edition, the reducing rinse following Schiff for PAS is 0.55% potassium metabisulfite (pg 137-138). For PAS/AB the reducing rinse is given as sodium metabisulfite (Carson pg 149). My other texts (Sheehan/Hrapchak as well as Vacca) list the reducing rinse as sodium metabisulfite which makes sense to me since the Schiff is made with sodium metabisulfite. Is this a printing error in the Carson book? I appreciate any light anybody can shed on this. -- Angela Lamberth Histology Technician III Histology Core Lab La Jolla Institute for Allergy & Immunology 9420 Athena Circle La Jolla, CA 92037 From tejohnson at genoptix.com Mon Mar 6 12:06:19 2017 From: tejohnson at genoptix.com (Teri Johnson) Date: Mon, 6 Mar 2017 18:06:19 +0000 Subject: [Histonet] TUNEL Message-ID: Hi Bernice, Likely there is something in your TUNEL procedure that is causing a problem with histochemical nuclear staining. If it isn't the pre-treatment, it might be the DAPI. After all, they do bind the same structures, and the DAPI might be winning that competition. Teri Johnson Manager, Clinical Trial Testing T +1 760 516 5954 M +1 442 222 4503 tejohnson at genoptix.com Navigate BioPharma, Inc. A Novartis Company 1890 Rutherford Rd. Carlsbad, CA 92008 USA ________________________________ CONFIDENTIALITY NOTICE: The sender of this email is not an employee or agent of, or a contractor or consultant to, Genoptix. This email has been sent through the Genoptix email system as a transitional service provided by Genoptix, Inc. ("Genoptix") to Navigate BioPharma Services, Inc. The sender of this email is not authorized to represent or to speak on behalf of any member of Genoptix, nor to enter into any undertaking, arrangement or agreement on behalf of any member of Genoptix. Any such undertaking, arrangement or agreement shall not be binding on Genoptix. Genoptix accepts no liability for the content of this email, or for the consequences of any acts or omissions taken or not taken on the basis of the information contained in this email or in any email sent from this email address. All reasonable precautions have been taken to ensure no viruses or other malware are present in this e-mail and Genoptix cannot accept responsibility for any loss or damage arising from the use of this e-mail or attachments and you are responsible for ensuring that your own virus screening procedures are up to date and fit for purpose. From scrochiere at nedlc.com Mon Mar 6 12:33:56 2017 From: scrochiere at nedlc.com (Steven Crochiere) Date: Mon, 6 Mar 2017 13:33:56 -0500 Subject: [Histonet] UV bulb in cryostat Message-ID: <000a01d296a8$37461e60$a5d25b20$@nedlc.com> I am trying to justify the $1600 cost of replacing the uv bulb in one of my cryostats. The 2 other cryostats I have do not even have a uv bulb in them, but I have one tech who is insisting that I replace the bulb. I have been told by management that it's too expensive and since the other instruments don't have one, unnecessary Any thoughts p.s. I am siding with management on this one. s From philip_manfre at merck.com Mon Mar 6 13:01:27 2017 From: philip_manfre at merck.com (Manfre, Philip) Date: Mon, 6 Mar 2017 19:01:27 +0000 Subject: [Histonet] Metabisulfite rinse for PAS reaction (potassium vs sodium) In-Reply-To: References: Message-ID: I almost always have used SODIUM metabisulfite for any version of PAS. I am also familiar with procedures that go straight to tap water for the pot-Schiff's rinsing, though that was from a job in my past. I seem to remember that working fine. Phil. Philip Manfre, HT (ASCP) Associate Principal Scientist Merck Research Laboratories WP81-406 770 Sumneytown Pike West Point, PA 19486 215-652-9750 215-993-0383 (fax) philip_manfre at merck.com -----Original Message----- From: Angela Lamberth via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, March 06, 2017 12:58 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Metabisulfite rinse for PAS reaction (potassium vs sodium) In Carson?s 3rd edition, the reducing rinse following Schiff for PAS is 0.55% potassium metabisulfite (pg 137-138). For PAS/AB the reducing rinse is given as sodium metabisulfite (Carson pg 149). My other texts (Sheehan/Hrapchak as well as Vacca) list the reducing rinse as sodium metabisulfite which makes sense to me since the Schiff is made with sodium metabisulfite. Is this a printing error in the Carson book? I appreciate any light anybody can shed on this. -- Angela Lamberth Histology Technician III Histology Core Lab La Jolla Institute for Allergy & Immunology 9420 Athena Circle La Jolla, CA 92037 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From alamberth at lji.org Mon Mar 6 13:12:47 2017 From: alamberth at lji.org (Angela Lamberth) Date: Mon, 6 Mar 2017 11:12:47 -0800 Subject: [Histonet] Metabisulfite rinse for PAS reaction (potassium vs sodium) In-Reply-To: References: Message-ID: Thank you Philip. I'm writing a procedure and was driving myself bananas. On Mon, Mar 6, 2017 at 11:01 AM, Manfre, Philip wrote: > I almost always have used SODIUM metabisulfite for any version of PAS. I > am also familiar with procedures that go straight to tap water for the > pot-Schiff's rinsing, though that was from a job in my past. I seem to > remember that working fine. > > Phil. > > Philip Manfre, HT (ASCP) > Associate Principal Scientist > Merck Research Laboratories > WP81-406 > 770 Sumneytown Pike > West Point, PA 19486 > > 215-652-9750 > 215-993-0383 (fax) > philip_manfre at merck.com > > > > -----Original Message----- > From: Angela Lamberth via Histonet [mailto:histonet at lists. > utsouthwestern.edu] > Sent: Monday, March 06, 2017 12:58 PM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Metabisulfite rinse for PAS reaction (potassium vs > sodium) > > In Carson?s 3rd edition, the reducing rinse following Schiff for PAS is > 0.55% potassium metabisulfite (pg 137-138). For PAS/AB the reducing rinse > is given as sodium metabisulfite (Carson pg 149). > > > > My other texts (Sheehan/Hrapchak as well as Vacca) list the reducing rinse > as sodium metabisulfite which makes sense to me since the Schiff is made > with sodium metabisulfite. > > > > Is this a printing error in the Carson book? I appreciate any light anybody > can shed on this. > > -- > Angela Lamberth > Histology Technician III > Histology Core Lab > La Jolla Institute for Allergy & Immunology > 9420 Athena Circle > La Jolla, CA 92037 > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Notice: This e-mail message, together with any attachments, contains > information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, > New Jersey, USA 07033), and/or its affiliates Direct contact information > for affiliates is available at > http://www.merck.com/contact/contacts.html) that may be confidential, > proprietary copyrighted and/or legally privileged. It is intended solely > for the use of the individual or entity named on this message. If you are > not the intended recipient, and have received this message in error, > please notify us immediately by reply e-mail and then delete it from > your system. > -- Angela Lamberth Histology Technician III Histology Core Lab La Jolla Institute for Allergy & Immunology 9420 Athena Circle La Jolla, CA 92037 From relia1 at earthlink.net Mon Mar 6 13:42:50 2017 From: relia1 at earthlink.net (Pam Barker) Date: Mon, 6 Mar 2017 14:42:50 -0500 Subject: [Histonet] Posting for a friend. Unlicensed PA's and what they can qualify to gross Message-ID: <0dc501d296b1$d8ee9d60$8acbd820$@earthlink.net> Hi Histonetters!! I am posting these questions for a friend: What can a non licensed person with 12 or more science credits gross in a surgical pathology lab? What can a licensed Histotech with a 2 year degree, with the training from a Pathologist qualify to gross in a surgical Pathology lab? What are the Florida state requirements as well as CAP, CLIA and any other regulating agency? Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From joyce.weems at emoryhealthcare.org Mon Mar 6 15:35:05 2017 From: joyce.weems at emoryhealthcare.org (Weems, Joyce K.) Date: Mon, 6 Mar 2017 21:35:05 +0000 Subject: [Histonet] Shandon Cytoblock Message-ID: Hi Folks, Does anyone have any experience/advice/feedback on the Shandon Cytoblock for cell block preparation? TIA - j Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax 770-380-8099 Cell joyce.weems at emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From j.benavides at eae.csic.es Mon Mar 6 16:18:19 2017 From: j.benavides at eae.csic.es (Julio Benavides) Date: Mon, 06 Mar 2017 23:18:19 +0100 Subject: [Histonet] Trimming soft brain In-Reply-To: References: Message-ID: <20170306231819.Horde.yLnzqmg1byVn79kW7me1SA1@webmail.csic.es> Hi there, I?m trying to trim ovine foetal brain (90 days of gestation). The brain is too soft and there is no way to get a proper slice. It has been in buffered 10% formalin for three weeks but still too soft. When you take the brain out of the pot and put into the trimming table, it just kind of spill over the place. We have tried to embed the brain in 7.5% gelatin, which helps to keep the shape of the brain before trimming, but as soon you cut the slice, the centre of the tissue just melt away. I was wondering if there is any post fixative solution we can try. May be Bouin?s? Any suggestion would be greatly appreciated! Cheers Julio From mhale at MiracaLS.com Mon Mar 6 18:45:08 2017 From: mhale at MiracaLS.com (Hale, Meredith) Date: Tue, 7 Mar 2017 00:45:08 +0000 Subject: [Histonet] AZ HT Position Message-ID: <0E828EC51C7CC445A51E53F81B64E8C7BEB22D9A@DFW-MPEXCH01.pathologypartners.intranet> HISTOTECHNICIAN Chandler Pathology Services - Chandler, AZ Job Type: Full-time Reports to: Laboratory Manager Schedule: 40 Hours per Week Salary: DOE FLSA Status: Hourly; Non-Exempt Essential functions and responsibilities: * Ensures compliance with all local, federal, CLIA, regulations. * Performs routine and non-routine activities involved in the preparation of slides for microscopic evaluation by pathologist(s), according to policies and procedures. * Responsible for embedding of surgical pathology specimens. * Responsible for the sectioning and microtomy of surgical pathology specimens. * Responsible for the routine staining, histochemical staining, and immunohistochemical staining of surgical pathology specimens for microscope evaluation and analysis. * Performs routine maintenance and cleaning of equipment and troubleshoots minor equipment failures. Documents remedial actions such as repairs or repeated tests. * Adheres to laboratory's quality control policies and documents all quality control activities. * Maintains diagnostic viability of all specimens and ensures correct patient labeling. * Unpacks orders received; dates and stocks orders, in accordance with established policies and procedures. * Maintain a clean and safe prep work area in accordance with all laboratory and safety SOP's. * Prepares and labels necessary stains and reagents in accordance with departmental procedures, policies and standards. * Reviews and performs QC on slides before taking the slides to the pathologist. * Maintenance of accurate work records and logs (i.e., Discrepancy Logs, Maintenance Logs, Quality Control Logs etc.) * Demonstrates a commitment to service, organizational values, and professionalism through appropriate conduct and demeanor at all times. * Works collaboratively and supports efforts of team members. * Protects patient information by adhering to professional standards including the Health Information Portability and Accountability Act (HIPAA). Knowledge and Skill: * Must have 2+ years laboratory experience. * Must be HT Certified. * Must be precise when performing technical tasks. * Effective interpersonal skills, both in person and on the telephone. * High accuracy in work and attention to detail. Physical demands: * Must have manual dexterity and motor coordination. * May involve sitting, standing, walking for long periods of time and/ or sitting at the microtome for long periods of time. * Exposure to chemicals and fumes. Benefit Package: * Health, Dental and Vision Insurance, 401K, Paid time off and Holiday Pay. Required education: * Associate's Degree or higher Required experience and certification: * laboratory: 2 years * HT Certification ASCP Chandler Pathology Services is an Equal Opportunity Employer. This company does not and will not discriminate in employment and personnel practices on the basis of race, sex, age, handicap, religion, national origin or any other basis prohibited by applicable law. Hiring, transferring and promotion practices are performed without regard to the above listed items. To apply, please fax resume to (480) 786-6996, ATTN: Office Manager or email: jobposting2600 at gmail.com From llewllew at shaw.ca Mon Mar 6 20:28:06 2017 From: llewllew at shaw.ca (Bryan Llewellyn) Date: Mon, 6 Mar 2017 18:28:06 -0800 Subject: [Histonet] Metabisulfite rinse for PAS reaction (potassium vs sodium) In-Reply-To: References: Message-ID: <1ede4ef1-c2a4-71c9-9df1-85cb4945c076@shaw.ca> You can use either the sodium or potassium salt. Both can also be used to make Schiff's reagent as well. In fact, many histotechs leave out the sulphite rinse step and simply rinse off in water and wash well with tap water. It seems to work just as well. Bryan Llewellyn Angela Lamberth via Histonet wrote: > In Carson?s 3rd edition, the reducing rinse following Schiff for PAS is > 0.55% potassium metabisulfite (pg 137-138). For PAS/AB the reducing rinse > is given as sodium metabisulfite (Carson pg 149). > > > > My other texts (Sheehan/Hrapchak as well as Vacca) list the reducing rinse > as sodium metabisulfite which makes sense to me since the Schiff is made > with sodium metabisulfite. > > > > Is this a printing error in the Carson book? I appreciate any light anybody > can shed on this. > From jqb7 at cdc.gov Tue Mar 7 09:13:58 2017 From: jqb7 at cdc.gov (Sanders, Jeanine (CDC/OID/NCEZID)) Date: Tue, 7 Mar 2017 15:13:58 +0000 Subject: [Histonet] malarial pigment Message-ID: <2780a0c218634be1b835f22e7b0ffe5d@cdc.gov> Good morning! Does anyone know of a method using ammonium hydroxide to remove malarial pigment from tissue sections? Thanks! Jeanine H. Sanders Infectious Diseases Pathology Branch Centers for Disease Control and Prevention 1600 Clifton Rd., NE MS-G32 Atlanta, GA 30329 From relia1 at earthlink.net Tue Mar 7 09:18:53 2017 From: relia1 at earthlink.net (Pam Barker) Date: Tue, 7 Mar 2017 10:18:53 -0500 Subject: [Histonet] Here is a link to some cool ideas to celebrate Histotechnology Professionals Day March 10, 2017. Message-ID: <124801d29756$21becde0$653c69a0$@earthlink.net> Hi Histonetters!! This Friday March 10 is Histotechnology Professionals Day!! The National Society for Histotechnology has some great suggestions for ways to celebrate. Here is the link: http://www.nsh.org/content/histotechnology-professionals-day Happy Histotechnology Professionals Day! From RELIA! This year we mark 12 years serving the Histology community as the only recruiting firm working exclusively in nationwide permanent placement of histology professionals! We look forward to many more years together!! As a matter of fact I have some exciting opportunities in: California Texas Tennessee North Carolina New Jersey Wisconsin New York Colorado I have histotech , lead tech and management positions, day and night shifts IHC and routine histology and new opportunities coming in all of the time! All of these positions are full time and permanent and my clients and their teams can't wait to welcome you aboard! For more info for you or a friend (refer a friend earn a referral fee) Contact me!! You can reach via email at relia1 at earthlink.net or on my cell/text at 407-353-5070 or toll free at the office at 866-607-3542. Thank you for your time, your business, your support and your fellowship! Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From FMonson at wcupa.edu Tue Mar 7 09:28:52 2017 From: FMonson at wcupa.edu (Monson, Frederick) Date: Tue, 7 Mar 2017 15:28:52 +0000 Subject: [Histonet] Metabisulfite rinse for PAS reaction (potassium vs sodium) In-Reply-To: <1ede4ef1-c2a4-71c9-9df1-85cb4945c076@shaw.ca> References: <1ede4ef1-c2a4-71c9-9df1-85cb4945c076@shaw.ca> Message-ID: And, I always rinsed the Schiff with 'absolute' HOH before the tap water rinse - which rinses were taken from my protocol for Ehrlich's 'Acid' Hematoxylin that was mixed from scratch and aged in the second story deep window sills on the West wall of the Biology/Geology building at Lehigh University - usually left, after mixing, with a cotton plug, for around 6 months [with periodic compensatory 'absolute' water additions to the bottle]. A. Ageless NOTE: One of the most important phrases in organic chemistry is 'tautomeric shift.' Especially for biologists - most of whom never heard the phrase. Follow-up after GOOGLE: Why is tritium so dangerous...? B. Current NOTE: 98% of science folk - after decades of programming - don't know how many digital cameras they carry with them every day. C. HISTO_EXPERIMENT: next time sections are taking fresh, UN-fixed tissues, in the cryostat, and when time permits, do the following. 1. set 5 or more sections on glass slides - or coverslips - and set them aside IN the cryostat 2. immediately remove one and after thawing - very little time - and NOT treating with PA, immerse the specimen directly in Schiff's solution for the standard time and follow with the usual rinses and counter dye(s)-BUT, I left them out! 3. at one hour intervals repeat (2) with one of the remaining sections. 4. when you want a counter dye, please help yourself. 5. if the outcome resembles mine in 1968, explain what you have observed. Cheers to all, Fred Monson On the 16th of June, 16 days from my 78th, I will retire to do other things. That, at least, is the plan!! Frederick C. Monson, PhD Technical Director Center for Microanalysis and Imaging, Research and Training (CMIRT) West Chester University of PA Geology-Astronomy 750 South Church St. West Chester, PA, 19383 fmonson at wcupa.edu 610-738-0437(Work) -----Original Message----- From: Bryan Llewellyn via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, March 06, 2017 9:28 PM To: Histonet Subject: Re: [Histonet] Metabisulfite rinse for PAS reaction (potassium vs sodium) You can use either the sodium or potassium salt. Both can also be used to make Schiff's reagent as well. In fact, many histotechs leave out the sulphite rinse step and simply rinse off in water and wash well with tap water. It seems to work just as well. Bryan Llewellyn Angela Lamberth via Histonet wrote: > In Carson?s 3rd edition, the reducing rinse following Schiff for PAS > is 0.55% potassium metabisulfite (pg 137-138). For PAS/AB the reducing > rinse is given as sodium metabisulfite (Carson pg 149). > > > > My other texts (Sheehan/Hrapchak as well as Vacca) list the reducing > rinse as sodium metabisulfite which makes sense to me since the Schiff > is made with sodium metabisulfite. > > > > Is this a printing error in the Carson book? I appreciate any light > anybody can shed on this. > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From paula at excaliburpathology.com Tue Mar 7 10:03:25 2017 From: paula at excaliburpathology.com (Paula Keene Pierce) Date: Tue, 7 Mar 2017 16:03:25 +0000 (UTC) Subject: [Histonet] malarial pigment In-Reply-To: <2780a0c218634be1b835f22e7b0ffe5d@cdc.gov> References: <2780a0c218634be1b835f22e7b0ffe5d@cdc.gov> Message-ID: <2029191732.377706.1488902605196@mail.yahoo.com> Lee Luna's third edition. Page 43, Method II 1. Deparaffinize and hydrate to water.2. Wash in distilled water.3. Immerse slides in 100 ml of 70% alcohol, to which has been added 2 ml of 28% ammonium hydroxide, for 3 hours.4. Rinse in water.5. Rinse in 1% glacial acetic acid.6. Wash well in distilled water and stain as desired.?Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953FAX 405-759-7513www.excaliburpathology.com On Tuesday, March 7, 2017 9:46 AM, "Sanders, Jeanine (CDC/OID/NCEZID) via Histonet" wrote: Good morning! Does anyone know of a method using ammonium hydroxide to remove malarial pigment from tissue sections? Thanks! Jeanine H. Sanders Infectious Diseases Pathology Branch Centers for Disease Control and Prevention 1600 Clifton Rd., NE MS-G32 Atlanta, GA 30329 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From melissa at alliedsearchpartners.com Tue Mar 7 10:06:46 2017 From: melissa at alliedsearchpartners.com (Melissa Owens) Date: Tue, 7 Mar 2017 16:06:46 +0000 Subject: [Histonet] Histology Tech Job in New York (Will take NY License Eligibility) Message-ID: Good Morning, I have a Histotech position in New York near Queens area and I am able to take someone who is New York License eligible. The shift is Tuesday-Saturday 11pm-7:30am. The position comes with vacation/sick days, paid holidays, medical/dental/vision and other benefits! This is a permanent full time position. ASCP not required and NY license eligibility accepted. Must have 1-2 years of histology experience. Please contact me for more details. Melissa Owens President, Laboratory Staffing Allied Search Partners T: 888.388.7571 ext. 102 F: 888.388.7572 From angie at ka-recruiting.com Tue Mar 7 12:20:56 2017 From: angie at ka-recruiting.com (Angie Laparidis) Date: Tue, 7 Mar 2017 13:20:56 -0500 Subject: [Histonet] Updated Openings across the US Message-ID: Hello Histonet Subscribers, I wanted to send along my active openings list for some great Histotech opportunities across the US! Let me know what you are looking for in your next career move & please send over an updated resume. AR- (1st shift, starting at either 4:30AM, or 5AM) AR- (1st shift, Outreach & Dermatapothology lab) CA- Palm Springs area - FT 8 hr shift Mohs Histotech, M-F GA- Atlanta- (3rd shift (11 pm to 7 am) IL- Rockford- (part time, 6am-2:30pm) IN- Outside of Chicago - Histotechnician - Midnight NC- Durham- IHC Tech - 2nd Shift (HTL certified with BS degree ? immunohistochemistry) (MUST HAVE BS) NC- 1st shift hours 6am-2:30pm, 3rd shift opening 10pm-6:30am NY - Buffalo - Histotech (days) NY - New York - Histotech (day shift) NY ? Long Island- Histotech (NYS license required)(Monday - Friday, 5am-1:30pm) OH ? Columbus- Histotech VA ? Richmond-Histotechnologist (days) VA ? Rural- (rotating shift) WI ? Green Bay- (2nd & 3rd shifts) Look forward to hearing from you! Sincerely, *Angie Laparidis*Healthcare Recruiter K.A. Recruiting, Inc. 10 Post Office Square, 8th Floor South, Boston, MA, 02109 W: 617.746-2744 (*please note this is a new number*) F: (617) 507-8009 Angie at ka-recruiting.com Our openings are updated daily at www.ka-recruiting.com From phruntedeske at gmail.com Tue Mar 7 12:21:51 2017 From: phruntedeske at gmail.com (Keith Brown) Date: Tue, 7 Mar 2017 13:21:51 -0500 Subject: [Histonet] Thank you Message-ID: Yesterday, I posted on this list some very basic questions about how to get started in this field as a second career, having spent the last nearly two decades in the news business. The number of people who responded, on-list and off, was surprising. And the honest efforts from people I've never met to try to help a guy get on the right track was much appreciated. Each one. I wanted to say thanks to everyone. I didn't expect that kind of response and if that's any indication of the people who work in this field, I think I've made the right decision about the discipline to attempt breaking into. Thanks a lot, Keith. From Richard.Cartun at hhchealth.org Tue Mar 7 17:23:01 2017 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Tue, 7 Mar 2017 23:23:01 +0000 Subject: [Histonet] Serotonin Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E953F7C6A@HHCEXCHMB03.hhcsystem.org> Can anyone recommend a good anti-Serotonin antibody for diagnostic morphologic proteomics testing (aka, Immunohistochemistry)? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From BZIMMERM at augusta.edu Thu Mar 9 13:47:48 2017 From: BZIMMERM at augusta.edu (Zimmerman, Billie) Date: Thu, 9 Mar 2017 19:47:48 +0000 Subject: [Histonet] Histopalooza GSH April 21 - 23 Legacy Lodge, Lake Lanier Buford GA Message-ID: Just a friendly reminder to register for Histopalooza by March 21st. Here's the link for registration: http://www.histosearch.com/gsh/2017GSHReg.pdf What a steal of a deal! Price of 3 days is $135 and if registering prior to April 10th. (after April 10th it's $150) The first day (Friday) is an eight hour prep class for passing the HT/HTL exam taught by Ely Klar. Saturday there will be all day classes and on Sunday class until noon. Bring the family because there's something for every age group here at Legacy Lodge. Make it a mini vacation while getting your CEUs. Hope to see all of you soon. Billie Zimmerman GSH PR From diane.satterfield at duke.edu Thu Mar 9 13:56:09 2017 From: diane.satterfield at duke.edu (Diane Satterfield) Date: Thu, 9 Mar 2017 19:56:09 +0000 Subject: [Histonet] Eosin used in processing Message-ID: For those of you that use Eosin in the processing of tissue. Can you please tell me what kind of Eosin mixture you use and how much? Thanks Diane From ssegal2 at slu.edu Thu Mar 9 14:56:14 2017 From: ssegal2 at slu.edu (Salomao Segal) Date: Thu, 9 Mar 2017 14:56:14 -0600 Subject: [Histonet] (no subject) Message-ID: I would appreciate if anyone could direct me to protocols for processing large slabs of tissue such as whole human brain slabs. I have acquired a very large microtome and knife but no previous experience with embedding, cutting, mounting and cover slipping such large sections. Any information/protocols/resources would be greatly appreciated. *Solomon Segal, M.D.* *Associate Professor of Anatomy Department of AnatomyKirksville College of Osteopathic MedicineA.T. Still University* *800 W. Jefferson St.* *Kirksville, MO 63501-1497* *e-mail*: s olomonsegal at atsu.edu www.atsu.edu From leoncinis44 at yahoo.com Thu Mar 9 14:57:47 2017 From: leoncinis44 at yahoo.com (Maria Leoncini) Date: Thu, 9 Mar 2017 20:57:47 +0000 (UTC) Subject: [Histonet] V.I.P E300 References: <1958602239.2372136.1489093067013.ref@mail.yahoo.com> Message-ID: <1958602239.2372136.1489093067013@mail.yahoo.com> ? Our lab bought one in Mercedes medical.this is the phone: 800.331.2716I hope I've helped From melissa at alliedsearchpartners.com Thu Mar 9 15:10:07 2017 From: melissa at alliedsearchpartners.com (Melissa Owens) Date: Thu, 9 Mar 2017 21:10:07 +0000 Subject: [Histonet] Need a Vacation Fill In Histotech in Aurora, CO Message-ID: Hello, I am in search of a vacation fill in for May 1st-May 23rd. This is a Day Shift opportunity, M-TH, 4/10s from 7am-3:30pm. If you are in the Aurora, CO area and have the ability and desire to do this than please reach out to me for more information. Thank you! Melissa Owens President, Laboratory Staffing Allied Search Partners T: 888.388.7571 ext. 102 F: 888.388.7572 From joyce.weems at emoryhealthcare.org Thu Mar 9 15:10:41 2017 From: joyce.weems at emoryhealthcare.org (Weems, Joyce K.) Date: Thu, 9 Mar 2017 21:10:41 +0000 Subject: [Histonet] Peloris and Eosin Message-ID: Hello All, For those of you using Peloris processors, what do you use instead of Eosin to dye your biopsies for better visibility at embedding? Inquiring minds... TIA! j Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax 770-380-8099 Cell joyce.weems at emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From dr at personifysearch.com Thu Mar 9 15:32:42 2017 From: dr at personifysearch.com (Danielle Robinson) Date: Thu, 9 Mar 2017 16:32:42 -0500 Subject: [Histonet] Clinical Pathology Technician II Opportunities- Reno, NV and Greater Boston Area Message-ID: Good Afternoon, Our exclusively retained client, a world leading CRO, is searching for Clinical Pathology Technicians in Reno, NV and Shrewsbury, MA. These are full-time, direct-hire positions with great benefits and opportunity for growth. Technicians may cross-train in other areas of pathology (clinical, special, bio-analytical) and perform histology procedures. If anyone is interested in learning more about these positions, please contact me directly at dr at personifysearch.com Thank you! -- Danielle Robinson Team Lead, Talent Acquisition *Personify* 416 S. Dawson Street Raleigh, NC 27601 (Direct) 919.460.8726 www.personifysearch.com -- CONFIDENTIALITY NOTICE: The contents of this email message and any attachments are intended solely for the addressee(s) and may contain confidential and/or privileged information and may be legally protected from disclosure. If you are not the intended recipient of this message or their agent, or if this message has been addressed to you in error, please immediately alert the sender by reply email and then delete this message and any attachments. If you are not the intended recipient, you are hereby notified that any use, dissemination, copying, or storage of this message or its attachments is strictly prohibited. From relia1 at earthlink.net Fri Mar 10 08:32:54 2017 From: relia1 at earthlink.net (Pam Barker) Date: Fri, 10 Mar 2017 09:32:54 -0500 Subject: [Histonet] Happy Histotechnology Professionals Day!! #hpd Message-ID: <006001d299ab$35159570$9f40c050$@earthlink.net> Hi Histonetters!! Happy Histotechnology Day!! If you want to see how your fellow histotechs are celebrating today follow the hashtag #hpd on social media - Facebook, LinkedIn and Instagram! Whatever you do today it's your day and I hope it's special! You are the unsung heroes of healthcare and today is your day to sing!!! Thanks-Pam p.s TGIF!! Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From melissa at alliedsearchpartners.com Fri Mar 10 13:22:27 2017 From: melissa at alliedsearchpartners.com (Melissa Owens) Date: Fri, 10 Mar 2017 19:22:27 +0000 Subject: [Histonet] Happy Histotechnology Professionals Day! Message-ID: Happy Histotechnology Professionals Day! I am very proud to say I have had the pleasure of being a part of this industry now for a decade (and counting). Enjoy your Day! Here is a list of our current Anatomic Pathology positions as well! Have a great weekend. Reach out to me directly for more information! 1. Pathologist-Delaware 2. Clinical Pathology Research Director-New Jersey 3. Histotech-Wisconsin 4. Histotech-Southwest Florida 5. Cytotechnologist-New York (Long Island) 6. Histotech-Western Colorado 7. Histology Supervisor-Southern California 8. MOHS Technician-Minnesota 9. Histotech-New York (East of Queens) 10. MOHS Technician-Southwest Florida 11. Cytotechnologist-Delaware Melissa Owens President, Laboratory Staffing Allied Search Partners T: 888.388.7571 ext. 102 F: 888.388.7572 From j.rowaihi at alborglaboratories.com Sat Mar 11 07:38:12 2017 From: j.rowaihi at alborglaboratories.com (Jamal Rwaihi) Date: Sat, 11 Mar 2017 16:38:12 +0300 Subject: [Histonet] SOP share Message-ID: <004c01d29a6c$bbd687b0$33839710$@alborglaboratories.com> Hi colleagues I hope all are fine Please share me the SOP for: 1. Leica ASP6025 Automatic Tissue Processor. 2. Tissue-Tek Prisma & Film Automated Slide Stainer & Film Coverslipper. 3. Leica IP C Inkjet Printer for Tissue Cassettes. Best Regards, Jamal M. Al Rowaihi Al Borg Medical Laboratories I Anatomic Pathology Supervisor I Headquarters, Jeddah, KSA I Phone: +966 12 670 0099 I Mobile +966 503629832 www.alborglaboratories.com From rsrichmond at gmail.com Sat Mar 11 12:20:52 2017 From: rsrichmond at gmail.com (Bob Richmond) Date: Sat, 11 Mar 2017 13:20:52 -0500 Subject: [Histonet] Happy Histotechnology Professionals Day! Message-ID: The old Samurai Pathologist - now retiring at 78 - thanks the many histotechnologists who've kept him out of hot water the past 52 years. Too bad we can't have more kids in search of a career reading Histonet - or at least, the Help Desperately Needed notices! Bob Richmond Samurai Pathologist Maryville TN From GenieJacobs at texashealth.org Sun Mar 12 11:56:33 2017 From: GenieJacobs at texashealth.org (Jacobs, Genie) Date: Sun, 12 Mar 2017 16:56:33 +0000 Subject: [Histonet] Leica ASP Message-ID: Looking to purchase refurbished ASP or ASP S basically for Biopsies. Any pros or cons.Need a 2 hour run that is really 2 hours. Thanks The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from disclosure under applicable law. If you are not the intended recipient, you are prohibited from copying, distributing, or using the information. Please contact the sender immediately by return e-mail and delete the original message from your system. From kenu2 at comcast.net Sun Mar 12 12:46:20 2017 From: kenu2 at comcast.net (KEN URBAN) Date: Sun, 12 Mar 2017 12:46:20 -0500 Subject: [Histonet] Histonet Digest, Vol 160, Issue 12 Message-ID: <73ab9b5c-8bcf-4cdb-9bf1-ccde5385ddac@Ken-Urbans-iPhone> Take care Bob. Ya know, ya never really retire-:) Sent from XFINITY Connect Mobile App ------ Original Message ------ From: histonet-request at lists.utsouthwestern.edu To: Histonet Sent: March 12, 2017 at 12:00 PM Subject: Histonet Digest, Vol 160, Issue 12 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Happy Histotechnology Professionals Day! (Bob Richmond) 2. Leica ASP (Jacobs, Genie) ---------------------------------------------------------------------- Message: 1 Date: Sat, 11 Mar 2017 13:20:52 -0500 From: Bob Richmond To: "Histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Happy Histotechnology Professionals Day! Message-ID: Content-Type: text/plain; charset=UTF-8 The old Samurai Pathologist - now retiring at 78 - thanks the many histotechnologists who've kept him o ut of hot water the past 52 years. Too bad we can't have more kids in search of a career reading Histonet - or at least, the Help Desperately Needed notices! Bob Richmond Samurai Pathologist Maryville TN ------------------------------ Message: 2 Date: Sun, 12 Mar 2017 16:56:33 +0000 From: "Jacobs, Genie" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Leica ASP Message-ID: Content-Type: text/plain; charset="iso-8859-1" Looking to purchase refurbished ASP or ASP S basically for Biopsies. Any pros or cons.Need a 2 hour run that is really 2 hours. Thanks The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from disclosure under applicable law. If you are not the intended recipient, you are prohibited from copying, distributing, or using the information. Please contact the sender immediately by return e-mail and d elete the original message from your system. ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 160, Issue 12 ***************************************** From j.rowaihi at alborglaboratories.com Sun Mar 12 14:03:27 2017 From: j.rowaihi at alborglaboratories.com (Jamal Rowaihi) Date: Sun, 12 Mar 2017 22:03:27 +0300 Subject: [Histonet] SOP share Message-ID: No single response? Regards Jamal RowaihiAnatomic Pathology SupervisorAl Borg Medical Laboratories?Sent from my cell phone -------- Original message --------From: Jamal Rwaihi via Histonet Date: 3/11/17 4:38 PM (GMT+03:00) To: Histonet edu Subject: [Histonet] SOP share Hi colleagues I hope all are fine Please share me the SOP for: 1.??? Leica ASP6025 Automatic Tissue Processor. 2.??? Tissue-Tek Prisma & Film Automated Slide Stainer & Film Coverslipper. 3.??? Leica IP C Inkjet Printer for Tissue Cassettes. Best Regards, Jamal M. Al Rowaihi Al Borg Medical Laboratories I Anatomic Pathology Supervisor I Headquarters, Jeddah, KSA I Phone: +966 12 670 0099 I Mobile +966 503629832 www.alborglaboratories.com _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From j.rowaihi at alborglaboratories.com Mon Mar 13 06:28:51 2017 From: j.rowaihi at alborglaboratories.com (Jamal Rwaihi) Date: Mon, 13 Mar 2017 14:28:51 +0300 Subject: [Histonet] SOP share In-Reply-To: <2AE3386A-5CC8-4709-A435-80B57530DBE6@gmail.com> References: <2AE3386A-5CC8-4709-A435-80B57530DBE6@gmail.com> Message-ID: <006801d29bec$ff15eda0$fd41c8e0$@alborglaboratories.com> Hi Cristi As you know every machine has a Standard Operating Procedure which include: Operation, maintenance, troubleshooting, quality control, that's what I need. Mostly the laboratories are collecting those data from different sources in SOP, so if someone have it ready and willing to share me a copy from it will be great. Best Regards, Jamal M. Al Rowaihi Al Borg Medical Laboratories I Anatomic Pathology Supervisor I Headquarters, Jeddah, KSA I Phone: +966 12 670 0099 I Mobile +966 503629832 www.alborglaboratories.com -----Original Message----- From: Cristi Rigazio [mailto:cls71877 at gmail.com] Sent: Monday, March 13, 2017 12:32 AM To: Jamal Rowaihi Subject: Re: [Histonet] SOP share I am not sure I know what you are looking? We wrote the SOP based on the manual, set the procedure for processing and staining through validation and working with the pathologist to find a product they want and the cassette printer is set up based on what information you want printed ; last name accession number, bar code, etc., the SOP should cover how you use it, maintenance procedures and such...can you be more specific as to what you need? What tissue type, what do you want the stainer to do, etc., Thanks, Cristi Sent from my iPhone > On Mar 12, 2017, at 3:03 PM, Jamal Rowaihi via Histonet wrote: > > No single response > > > Regards > Jamal RowaihiAnatomic Pathology SupervisorAl Borg Medical Laboratories > Sent from my cell phone > -------- Original message --------From: Jamal Rwaihi via Histonet > Date: 3/11/17 4:38 PM > (GMT+03:00) To: Histonet edu > Subject: [Histonet] SOP share Hi colleagues > > I hope all are fine > > Please share me the SOP for: > > 1. Leica ASP6025 Automatic Tissue Processor. > > 2. Tissue-Tek Prisma & Film Automated Slide Stainer & Film Coverslipper. > > 3. Leica IP C Inkjet Printer for Tissue Cassettes. > > > > > > > > Best Regards, > > > > > > Jamal M. Al Rowaihi > > Al Borg Medical Laboratories I Anatomic Pathology Supervisor I > Headquarters, Jeddah, KSA I Phone: +966 12 670 0099 I Mobile +966 > 503629832 > > www.alborglaboratories.com > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gagnone at KGH.KARI.NET Mon Mar 13 09:14:08 2017 From: gagnone at KGH.KARI.NET (Gagnon, Eric) Date: Mon, 13 Mar 2017 14:14:08 +0000 Subject: [Histonet] Bob Richmond Message-ID: <5F06C3AD0B27264CA20CFA986C87882E01BB673CEB@EXCHANGEPV1.KGH.ON.CA> Happy 'retirement', Bob. Old pathologists never retire. They are just glassy-eyed! Appreciative of your many years of pragmatic, might I say homespun, advice and counsel on this list. Many of your posts have been printed off and retained in my Big Binder of Histo-Truth. Enjoy! Eric Gagnon Histology Laboratory Kingston General Hospital, Kingston, Ontario, Canada. >The old Samurai Pathologist - now retiring at 78 - thanks the many histotechnologists who've kept him out of hot water the past 52 years. Too bad we can't have more kids in search of a career reading Histonet - or at least, the Help Desperately Needed notices! Bob Richmond Samurai Pathologist Maryville TN From lblazek at digestivespecialists.com Mon Mar 13 09:45:56 2017 From: lblazek at digestivespecialists.com (Blazek, Linda) Date: Mon, 13 Mar 2017 10:45:56 -0400 Subject: [Histonet] Bob Richmond In-Reply-To: <5F06C3AD0B27264CA20CFA986C87882E01BB673CEB@EXCHANGEPV1.KGH.ON.CA> References: <5F06C3AD0B27264CA20CFA986C87882E01BB673CEB@EXCHANGEPV1.KGH.ON.CA> Message-ID: <5A2BD13465E061429D6455C8D6B40E39198A699D73@IBMB7Exchange.digestivespecialists.com> Happy retirement Dr. Richmond! Just don't retire from the histonet! We need you. I too have a wonderful Samurai collection that someday I'll hand down to a "newbie" that will just scratch their head and say "really????????" Your advice and humor have been appreciated (and collected) more than you know! Linda Linda Blazek HT (ASCP) Pathology Lab Manager GI Pathology of Dayton Digestive Specialists, Inc Phone: (937) 396-2623 Email: lblazek at digestivespecialists.com -----Original Message----- From: Gagnon, Eric via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, March 13, 2017 10:14 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Bob Richmond Happy 'retirement', Bob. Old pathologists never retire. They are just glassy-eyed! Appreciative of your many years of pragmatic, might I say homespun, advice and counsel on this list. Many of your posts have been printed off and retained in my Big Binder of Histo-Truth. Enjoy! Eric Gagnon Histology Laboratory Kingston General Hospital, Kingston, Ontario, Canada. >The old Samurai Pathologist - now retiring at 78 - thanks the many histotechnologists who've kept him out of hot water the past 52 years. Too bad we can't have more kids in search of a career reading Histonet - or at least, the Help Desperately Needed notices! Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From billodonnell at catholichealth.net Mon Mar 13 10:17:40 2017 From: billodonnell at catholichealth.net (O'Donnell, Bill) Date: Mon, 13 Mar 2017 15:17:40 +0000 Subject: [Histonet] SOP share In-Reply-To: References: Message-ID: Jamal, My response is to your response of " No single response" There are some things you need to be aware of: I work for an institution that will fire people for sharing an SOP with someone outside our system. They even block/encrypt e-mails with attachments from going out. I suspect a lot of folks here in US are in a similar situation. It's a different world in healthcare than it was even 8-10 years ago.. SOPs are considered the property of the employer - so I would be stealing from my company to give you an SOP. Another thing to understand is that most people work very hard on their SOPs and would rather not just hand over their work, especially to someone they don't know personally. That's all I have to say on the subject - have a nice week -----Original Message----- From: Jamal Rowaihi via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Sunday, March 12, 2017 2:03 PM To: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] SOP share CAUTION: This email is not from a CHI source. Only click links or open attachments you know are safe. ...................................................................... No single response? Regards Jamal RowaihiAnatomic Pathology SupervisorAl Borg Medical Laboratories?Sent from my cell phone -------- Original message --------From: Jamal Rwaihi via Histonet Date: 3/11/17 4:38 PM (GMT+03:00) To: Histonet edu Subject: [Histonet] SOP share Hi colleagues I hope all are fine Please share me the SOP for: 1.??? Leica ASP6025 Automatic Tissue Processor. 2.??? Tissue-Tek Prisma & Film Automated Slide Stainer & Film Coverslipper. 3.??? Leica IP C Inkjet Printer for Tissue Cassettes. Best Regards, Jamal M. Al Rowaihi Al Borg Medical Laboratories I Anatomic Pathology Supervisor I Headquarters, Jeddah, KSA I Phone: +966 12 670 0099 I Mobile +966 503629832 www.alborglaboratories.com _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwIGaQ&c=ND-Z_FnoJTOCBd6ZraMT0-wJD0GDS_U0VV_Zq7yxAI4&r=3iEA9oByFKGUVmHHjEGViq9-hi91LvAPvOKSxbQaAak&m=Aq3ceRNscpTxAg-Nssfkg5YAwcqXEKkZGJilVqFyhv8&s=RDuoPAYcwu5R76_TN2zW1VHDmb2fqHipprtsE42YWxw&e= _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwIGaQ&c=ND-Z_FnoJTOCBd6ZraMT0-wJD0GDS_U0VV_Zq7yxAI4&r=3iEA9oByFKGUVmHHjEGViq9-hi91LvAPvOKSxbQaAak&m=Aq3ceRNscpTxAg-Nssfkg5YAwcqXEKkZGJilVqFyhv8&s=RDuoPAYcwu5R76_TN2zW1VHDmb2fqHipprtsE42YWxw&e= This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. From FMonson at wcupa.edu Mon Mar 13 12:16:42 2017 From: FMonson at wcupa.edu (Monson, Frederick) Date: Mon, 13 Mar 2017 17:16:42 +0000 Subject: [Histonet] Happy Histotechnology Professionals Day! In-Reply-To: References: Message-ID: <1489425402471.28673@wcupa.edu> And I, "O>>>>>" scary one, is following your class by leaving 38 days BEFORE I will be 78. You really are old!?""@ In my family, retirement has appeared to lead directly - one way or another - to demise, and I have yet to discover anyone who has turned the inevitability of that ending. So, I have contrived to take advantage of all that time by doing things that previous family have avoided. 1. I have mapped out a morning walk that is 10 miles from my house but the required exact 1 mile - thus, I will return to my car when it is finished. 2. I will return to our home and ride my bike around the block that is NOT a mile and has no traffic. 3. I am purchasing a digital camera to capture images from all of my sections - kept from 1965 to the present. Since this activity is considered a punishment for putting it off for so long, it is clear that the effort will be life prolonging. 4. I have been practicing my aiming at 100 yards for several years now, and I will have time and energy to allot more time so that I can reach my goal of hitting the bull's eye with 5 rounds in 1 minute. I have already made it clear that I will not leave this effort undone for any reason. To make this effort fun, I will load my own rounds to a tenth (0.1) of a grain. {You would think that we would have left weighing by seeds behind us now that we have grams; but I guess we will go to any length to avoid using metrics. 4.a. I have never been punished for joining the USMC when I needed a vacation from from the rigors of Lehigh University. I enjoyed that hiatus so much that I have always called Paris Island, "Pleasure Island." My older son joined as well, but he calls it: "The bad place." He has placed his 5 rounds in the bull's eye many times, and that explains my goal to achieve it just once. 5. Finally, I have signed a witnessed paper, under duress by my wife, that I will not go first, and I do try not to break promises to her. I hope that you have a similar set of activities in your plan. However, if not, then would you like one of my ICS microtomes (at 2 micron intervals) to keep your hand in rotating (otherwise known as 'cranking')? BTW, has anyone EVER made a microtome for a lefty? Ah! If you were a lefty Bob, you would be a pathologist, because you were never comfortable cutting sections! Wow! I never thought of that before. Cheers and reciprocating best wishes, Fred Monson Frederick C Monson, PhD Center for Microanalysis and Imaging, Research and Training (CMIRT) West Chester University of Pannsylvania West Chester, PA, 1938 610-738-0437 fmonson at wcupa.edu ________________________________________ From: Bob Richmond via Histonet Sent: Saturday, March 11, 2017 1:20 PM To: Histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Happy Histotechnology Professionals Day! The old Samurai Pathologist - now retiring at 78 - thanks the many histotechnologists who've kept him out of hot water the past 52 years. Too bad we can't have more kids in search of a career reading Histonet - or at least, the Help Desperately Needed notices! Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From angie at ka-recruiting.com Mon Mar 13 15:24:32 2017 From: angie at ka-recruiting.com (Angie Laparidis) Date: Mon, 13 Mar 2017 16:24:32 -0400 Subject: [Histonet] Palm Springs MOHs tech Message-ID: Hi Histonetters! My client opened a new MOHS tech opportunity with a large reputable healthcare system in *Palm Springs, CA*! This is a M-F Day-Shift opportunity that will not be open very long!! Let me know if you can think of anyone who may be interested and send along an updated resume. Look forward to hearing from you! Sincerely, *Angie Laparidis*Healthcare Recruiter K.A. Recruiting, Inc. 10 Post Office Square, 8th Floor South, Boston, MA, 02109 W: 617.746-2744 (*please note this is a new number*) F: (617) 507-8009 Angie at ka-recruiting.com Our openings are updated daily at www.ka-recruiting.com From relia1 at earthlink.net Mon Mar 13 15:26:05 2017 From: relia1 at earthlink.net (Pam Barker) Date: Mon, 13 Mar 2017 16:26:05 -0400 Subject: [Histonet] Hope your Histotechnology Professionals Day was Great! Check out #hpd Message-ID: <004b01d29c38$0c678c20$2536a460$@earthlink.net> Hello Histonetters, I hope your week is off to a great start!!? I know mine is!! I want to remind you once more about the hashtag #hpd Search the hashtag on Facebook, Instagram and Twitter to see all the fun your fellow histotechs had on Histotechnology Professionals Day!! I would also like to tell you about the positions I am currently working on. Here are the hot jobs I want to share with you! RELIA Spotlight Histology Job!!! ? Histology Tech ? Austin, TX ? IHC Tech ? Modesto, CA ? Histology Tech ?Modesto, CA Here are the rest of my sizzlin? hot jobs!!! ? Histology Manager ? San Diego, CA ? Histology Manager ? Irvine, CA ? Histotechnician ? Wilmington, NC ? Histology Tech ? Cranford, NJ ? Histotech ? San Diego, CA ? Grossing Histotech ? Nashville, TN ? Histology Tech ? Kingsport, TN And new opportunities are coming in nationwide DAILY! If you are on the hunt and your desired location is not listed shoot me an email and tell me where you want to go so I can investigate for you.? REMEMBER ? ALL INQUIRIES ARE KEPT CONFIDENTIAL! Unlike OTHER recruiting firms; Here at RELIA I will only show a client YOUR resume with YOUR? permission. I respect YOU and YOUR career!! All of my clients offer excellent compensation, benefits and in most cases relocation assistance.? These are full time permanent positions with stable secure labs.? My clients are eager to interview and hire for these positions! If any of these opportunities are the right one for you RELIA can make it happen!!! If you or anyone you know is interested in any of these opportunities or are looking for a position in another area and want some help - Contact me!!!! I can be reached ASAP on my cell/text at 407-353-5070, via email at relia1 at earthlink.net? or toll free at the office at 866-607-3542. If you refer someone and I place them you will earn a referral fee!! Don?t forget to checkout #hpd Have a great week!!! Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From GenieJacobs at texashealth.org Mon Mar 13 15:48:00 2017 From: GenieJacobs at texashealth.org (Jacobs, Genie) Date: Mon, 13 Mar 2017 20:48:00 +0000 Subject: [Histonet] Leica ASP Message-ID: Looking to purchase a refurbished Leica ASP or ASPS. Is there a big difference. Need it for processing of biopsies. WE have VIPS but they do not rapid process. Their 2 hour run is really 31/2 with pump in time. Thanks The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from disclosure under applicable law. If you are not the intended recipient, you are prohibited from copying, distributing, or using the information. Please contact the sender immediately by return e-mail and delete the original message from your system. From tony.henwood at health.nsw.gov.au Mon Mar 13 18:02:32 2017 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Mon, 13 Mar 2017 23:02:32 +0000 Subject: [Histonet] Bob Richmond In-Reply-To: <5A2BD13465E061429D6455C8D6B40E39198A699D73@IBMB7Exchange.digestivespecialists.com> References: <5F06C3AD0B27264CA20CFA986C87882E01BB673CEB@EXCHANGEPV1.KGH.ON.CA> <5A2BD13465E061429D6455C8D6B40E39198A699D73@IBMB7Exchange.digestivespecialists.com> Message-ID: <0237449DE79DBC45B686AB82CDCD16FF014E86ED@SVDCMBX-MEX008.nswhealth.net> All the Best Bob, Have a great retirement. Maybe we will see you in Australia on one of those big boats that make their way here regularly. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From relia1 at earthlink.net Tue Mar 14 07:20:26 2017 From: relia1 at earthlink.net (Pam Barker) Date: Tue, 14 Mar 2017 08:20:26 -0400 Subject: [Histonet] Hope your Histotechnology Professionals Day was Great! Check out #hpd Message-ID: <005b01d29cbd$5ec5a990$1c50fcb0$@earthlink.net> Hello Histonetters, I hope your week is off to a great start!! I know mine is!! I want to remind you once more about the hashtag #hpd Search the hashtag on Facebook, Instagram and Twitter to see all the fun your fellow histotechs had on Histotechnology Professionals Day!! I would also like to tell you about the positions I am currently working on. Here are the hot jobs I want to share with you! RELIA Spotlight Histology Job!!! . Histology Tech - Austin, TX . IHC Tech - Modesto, CA . Histology Tech -Modesto, CA Here are the rest of my sizzlin' hot jobs!!! . Histology Manager - San Diego, CA . Histology Manager - Irvine, CA . Histotechnician - Wilmington, NC . Histology Tech - Cranford, NJ . Histotech - San Diego, CA . Grossing Histotech - Nashville, TN . Histology Tech - Kingsport, TN And new opportunities are coming in nationwide DAILY! If you are on the hunt and your desired location is not listed shoot me an email and tell me where you want to go so I can investigate for you. REMEMBER - ALL INQUIRIES ARE KEPT CONFIDENTIAL! Unlike OTHER recruiting firms; Here at RELIA I will only show a client YOUR resume with YOUR permission. I respect YOU and YOUR career!! All of my clients offer excellent compensation, benefits and in most cases relocation assistance. These are full time permanent positions with stable secure labs. My clients are eager to interview and hire for these positions! If any of these opportunities are the right one for you. RELIA can make it happen!!! If you or anyone you know is interested in any of these opportunities or are looking for a position in another area and want some help - Contact me!!!! I can be reached ASAP on my cell/text at 407-353-5070, via email at relia1 at earthlink.net or toll free at the office at 866-607-3542. If you refer someone and I place them you will earn a referral fee!! Don't forget to checkout #hpd Have a great week!!! Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From NMargaryan at luriechildrens.org Tue Mar 14 13:50:45 2017 From: NMargaryan at luriechildrens.org (Margaryan, Naira) Date: Tue, 14 Mar 2017 18:50:45 +0000 Subject: [Histonet] Immunoperoxidase Protocol on Cytospin Message-ID: <34B2EDA118548A4EB35D6E650345BA6469C067C8@SV-EX08.childrensmemorial.org> Dear histonetters: I never done ICC. A scientist brought me a slide with Cytospined cells on for IHC. I am going to fix with 10%NBF (it is only what I have now) then wash with TBST. May I skip AR step? May I use my usual IHC protocol and reagents which I usually use for FFPE tissue: blocks with H2O2, avidin/biotin/PB with TBST wash buffer???? Thanks in advance, Naira Ranked nationally in all 10 pediatric specialties by U.S. News & World Report (LCHOC Ver 1.0) This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. If verification is required please request a hard-copy version. (LCHOC VER 1.0) From Richard.Cartun at hhchealth.org Tue Mar 14 14:38:08 2017 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Tue, 14 Mar 2017 19:38:08 +0000 Subject: [Histonet] Immunoperoxidase Protocol on Cytospin In-Reply-To: <34B2EDA118548A4EB35D6E650345BA6469C067E1@SV-EX08.childrensmemorial.org> References: <34B2EDA118548A4EB35D6E650345BA6469C067C8@SV-EX08.childrensmemorial.org>, <9215BD4B0BA1B44D962A71C758B68D2E95401D98@HHCEXCHMB03.hhcsystem.org> <34B2EDA118548A4EB35D6E650345BA6469C067E1@SV-EX08.childrensmemorial.org> Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E95401DE3@HHCEXCHMB03.hhcsystem.org> Since you only have 1 slide I would recommend following your regular protocol that you use for formalin-fixed, paraffin-embedded tissue. I would use heat retrieval if that's what you use for FFPE tissue. However, if you use enzyme digestion on FFPE tissue I would "not" digest your cytospin slide. If you don't get any immunoreactivity it could be a "False Negative" due to pre-analytical variables. I think it can be interesting to try experiments like this, but it's not the way to do IHC testing. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax -----Original Message----- From: Margaryan, Naira [mailto:NMargaryan at luriechildrens.org] Sent: Tuesday, March 14, 2017 3:24 PM To: Cartun, Richard Subject: RE: Immunoperoxidase Protocol on Cytospin cytoplasmic staining for Nodal protein. thanks ________________________________________ From: Cartun, Richard [Richard.Cartun at hhchealth.org] Sent: Tuesday, March 14, 2017 2:13 PM To: Margaryan, Naira; histonet-bounces at lists.utsouthwestern.edu Subject: RE: Immunoperoxidase Protocol on Cytospin What is the target? Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax -----Original Message----- From: Margaryan, Naira via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, March 14, 2017 2:51 PM To: histonet-bounces at lists.utsouthwestern.edu Cc: histonet-request Subject: [Histonet] Immunoperoxidase Protocol on Cytospin Importance: High Dear histonetters: I never done ICC. A scientist brought me a slide with Cytospined cells on for IHC. I am going to fix with 10%NBF (it is only what I have now) then wash with TBST. May I skip AR step? May I use my usual IHC protocol and reagents which I usually use for FFPE tissue: blocks with H2O2, avidin/biotin/PB with TBST wash buffer???? Thanks in advance, Naira Ranked nationally in all 10 pediatric specialties by U.S. News & World Report (LCHOC Ver 1.0) This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. If verification is required please request a hard-copy version. (LCHOC VER 1.0) _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From Timothy.Morken at ucsf.edu Tue Mar 14 14:42:36 2017 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Tue, 14 Mar 2017 19:42:36 +0000 Subject: [Histonet] Congo red and LED illumination problem? Message-ID: <761E2B5697F795489C8710BCC72141FF8E5C19DF@ex07.net.ucsf.edu> Something new to me... A pathologist said he is getting reports that LED illumination on newer microscopes is leading to faint or even false negatives for congo red amyloid birefringence due to the difference in spectrum between LED and tungsten filament lamps. Has anyone else noticed this? Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center From melissa at alliedsearchpartners.com Tue Mar 14 19:47:09 2017 From: melissa at alliedsearchpartners.com (Melissa Owens) Date: Wed, 15 Mar 2017 00:47:09 +0000 Subject: [Histonet] Day Shift Histotech Job in Southern CA Message-ID: Hello Histoland, I have a Day Shift Histotech opportunity in Southern California for full time permanent employment. Please contact me directly for more details. Melissa Owens President, Laboratory Staffing Allied Search Partners T: 888.388.7571 ext. 102 F: 888.388.7572 From mbt137 at psu.edu Wed Mar 15 08:58:08 2017 From: mbt137 at psu.edu (MICHELLE BETH TITUNICK) Date: Wed, 15 Mar 2017 09:58:08 -0400 Subject: [Histonet] Bone Processing Message-ID: <1489586288l.21430310l.0l@psu.edu> Hello. Does anyone have a processing protocol for formalin fixed decalcified rat bones using an automated processor? I've been doing it manually with a vacuum oven and it takes 14-16 hours to complete. I don't have a vacuumed section on my automatic processor. Also any tips besides what I've read in the archives for immunohistochemistry? Thanks so much. Michelle B. Titunick PhD Candidate, Anatomy The Pennsylvania State University College of Medicine mbt137 at psu.edu (973) 715-9831 From BZIMMERM at augusta.edu Wed Mar 15 10:21:13 2017 From: BZIMMERM at augusta.edu (Zimmerman, Billie) Date: Wed, 15 Mar 2017 15:21:13 +0000 Subject: [Histonet] GSH Annual Histopalooza April 21st - April 23rd Legacy Lodge, Lake Lanier Islands, Georgia Message-ID: The Georgia Society for Histology Histopalooza is just around the corner!! Calling all procrastinators to register for the event. It's cheap, cheap, cheap compared to other CEU events. I spoke with Claudia Faulkenberry this morning. She will be speaking about the hot, hot antibody PDL1. (no, it ain't cheap ) It's on television commercials so the general public is asking about it. She will tell us the uses for this antibody as well as the various clones available. Companion diagnostics is the new buzz word for PDL1. Thank you Claudia. See you at Lake Lanier, Billie Zimmerman MT(ASCP)QIHC From abadesuyi at nrh-ok.com Wed Mar 15 12:38:52 2017 From: abadesuyi at nrh-ok.com (Adesupo, Adesuyi (Banjo)) Date: Wed, 15 Mar 2017 12:38:52 -0500 Subject: [Histonet] Training and Initial Competency Assessment Evaluation Message-ID: <04EE4F75BB5FB246ADB68D69B7460443B98877B427@MAIL.nrhnt.nrh-ok.com> Hi, Does anyone have a Training and Initial Competency Assessment Evaluation Checklist to share? Best regards, Adesupo Adesuyi ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. From alamberth at lji.org Wed Mar 15 12:39:37 2017 From: alamberth at lji.org (Angela Lamberth) Date: Wed, 15 Mar 2017 10:39:37 -0700 Subject: [Histonet] Folding/pinching in GI tissue Message-ID: Hello everyone, I can't find any reference to this particular artifact. There was a post from 2006 about it but no replies. I'm seeing numerous vertical folds or "pinches" in mouse GI tissue. The ffpe blocks have 3 strips and the artifact is not necessarily seen in all of the tissue; one GI strip might look beautiful under the scope and the remaining 2 (in the same block) will have these folds. One day everything will look flawless and other days (like today) many slides will exhibit this folding. I call them vertical folds because they are oriented in the direction of my cutting. Ribbons look great on the waterbath. I've tried preemptively "teasing" the sections with my forceps but that doesn't seem to help. Waterbath is 42C and sections are 4um. Blocks are nice and cold. I've tried increasing waterbath temp to 45 and floating sections for 20-30 seconds. I even tried slowing my cutting pace with this batch hoping that might make a difference. I'm really frustrated to keep seeing this under the microscope and slightly embarrassed that it keeps happening. What's really going on here and what more can I try? I've uploaded a pic (mouse ileum) to imgur for a visual reference. I took it with my iphone so it's not focused well but you'll be able to see what I'm talking about. https://imgur.com/a/IXoJG -- Angela Lamberth Histology Technician III Histology Core Lab La Jolla Institute for Allergy & Immunology 9420 Athena Circle La Jolla, CA 92037 From thigginsht at msn.com Wed Mar 15 12:46:39 2017 From: thigginsht at msn.com (T H) Date: Wed, 15 Mar 2017 17:46:39 +0000 Subject: [Histonet] Histonet Digest, Vol 160, Issue 15 In-Reply-To: References: Message-ID: When you fix the slide in 10% formalin you will have to perform AR. TH ________________________________ From: histonet-request at lists.utsouthwestern.edu Sent: Wednesday, March 15, 2017 12:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 160, Issue 15 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet Histonet Info Page - lists.utsouthwestern.edu Mailing Lists lists.utsouthwestern.edu Histonet -- For the exchange of information pertaining to histotechnology and related fields About Histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Immunoperoxidase Protocol on Cytospin (Margaryan, Naira) 2. Re: Immunoperoxidase Protocol on Cytospin (Cartun, Richard) 3. Congo red and LED illumination problem? (Morken, Timothy) 4. Day Shift Histotech Job in Southern CA (Melissa Owens) 5. Bone Processing (MICHELLE BETH TITUNICK) 6. GSH Annual Histopalooza April 21st - April 23rd Legacy Lodge, Lake Lanier Islands, Georgia (Zimmerman, Billie) ---------------------------------------------------------------------- Message: 1 Date: Tue, 14 Mar 2017 18:50:45 +0000 From: "Margaryan, Naira" To: "histonet-bounces at lists.utsouthwestern.edu" Cc: histonet-request Subject: [Histonet] Immunoperoxidase Protocol on Cytospin Message-ID: <34B2EDA118548A4EB35D6E650345BA6469C067C8 at SV-EX08.childrensmemorial.org> Content-Type: text/plain; charset="utf-8" Dear histonetters: I never done ICC. A scientist brought me a slide with Cytospined cells on for IHC. I am going to fix with 10%NBF (it is only what I have now) then wash with TBST. May I skip AR step? May I use my usual IHC protocol and reagents which I usually use for FFPE tissue: blocks with H2O2, avidin/biotin/PB with TBST wash buffer???? Thanks in advance, Naira Ranked nationally in all 10 pediatric specialties by U.S. News & World Report (LCHOC Ver 1.0) This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. If verification is required please request a hard-copy version. (LCHOC VER 1.0) ------------------------------ Message: 2 Date: Tue, 14 Mar 2017 19:38:08 +0000 From: "Cartun, Richard" To: "Margaryan, Naira" Cc: "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Immunoperoxidase Protocol on Cytospin Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E95401DE3 at HHCEXCHMB03.hhcsystem.org> Content-Type: text/plain; charset="us-ascii" Since you only have 1 slide I would recommend following your regular protocol that you use for formalin-fixed, paraffin-embedded tissue. I would use heat retrieval if that's what you use for FFPE tissue. However, if you use enzyme digestion on FFPE tissue I would "not" digest your cytospin slide. If you don't get any immunoreactivity it could be a "False Negative" due to pre-analytical variables. I think it can be interesting to try experiments like this, but it's not the way to do IHC testing. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax -----Original Message----- From: Margaryan, Naira [mailto:NMargaryan at luriechildrens.org] Sent: Tuesday, March 14, 2017 3:24 PM To: Cartun, Richard Subject: RE: Immunoperoxidase Protocol on Cytospin cytoplasmic staining for Nodal protein. thanks ________________________________________ From: Cartun, Richard [Richard.Cartun at hhchealth.org] Sent: Tuesday, March 14, 2017 2:13 PM To: Margaryan, Naira; histonet-bounces at lists.utsouthwestern.edu Subject: RE: Immunoperoxidase Protocol on Cytospin What is the target? Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax -----Original Message----- From: Margaryan, Naira via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, March 14, 2017 2:51 PM To: histonet-bounces at lists.utsouthwestern.edu Cc: histonet-request Subject: [Histonet] Immunoperoxidase Protocol on Cytospin Importance: High Dear histonetters: I never done ICC. A scientist brought me a slide with Cytospined cells on for IHC. I am going to fix with 10%NBF (it is only what I have now) then wash with TBST. May I skip AR step? May I use my usual IHC protocol and reagents which I usually use for FFPE tissue: blocks with H2O2, avidin/biotin/PB with TBST wash buffer???? Thanks in advance, Naira Ranked nationally in all 10 pediatric specialties by U.S. News & World Report (LCHOC Ver 1.0) This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. If verification is required please request a hard-copy version. (LCHOC VER 1.0) _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. ------------------------------ Message: 3 Date: Tue, 14 Mar 2017 19:42:36 +0000 From: "Morken, Timothy" To: Histonet Subject: [Histonet] Congo red and LED illumination problem? Message-ID: <761E2B5697F795489C8710BCC72141FF8E5C19DF at ex07.net.ucsf.edu> Content-Type: text/plain; charset="us-ascii" Something new to me... A pathologist said he is getting reports that LED illumination on newer microscopes is leading to faint or even false negatives for congo red amyloid birefringence due to the difference in spectrum between LED and tungsten filament lamps. Has anyone else noticed this? Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center ------------------------------ Message: 4 Date: Wed, 15 Mar 2017 00:47:09 +0000 From: Melissa Owens To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Day Shift Histotech Job in Southern CA Message-ID: Content-Type: text/plain; charset="us-ascii" Hello Histoland, I have a Day Shift Histotech opportunity in Southern California for full time permanent employment. Please contact me directly for more details. Melissa Owens President, Laboratory Staffing Allied Search Partners T: 888.388.7571 ext. 102 F: 888.388.7572 ------------------------------ Message: 5 Date: Wed, 15 Mar 2017 09:58:08 -0400 From: "MICHELLE BETH TITUNICK" To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Bone Processing Message-ID: <1489586288l.21430310l.0l at psu.edu> Content-Type: text/plain; charset=utf-8 Hello. Does anyone have a processing protocol for formalin fixed decalcified rat bones using an automated processor? I've been doing it manually with a vacuum oven and it takes 14-16 hours to complete. I don't have a vacuumed section on my automatic processor. Also any tips besides what I've read in the archives for immunohistochemistry? Thanks so much. Michelle B. Titunick PhD Candidate, Anatomy The Pennsylvania State University College of Medicine mbt137 at psu.edu (973) 715-9831 ------------------------------ Message: 6 Date: Wed, 15 Mar 2017 15:21:13 +0000 From: "Zimmerman, Billie" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] GSH Annual Histopalooza April 21st - April 23rd Legacy Lodge, Lake Lanier Islands, Georgia Message-ID: Content-Type: text/plain; charset="us-ascii" The Georgia Society for Histology Histopalooza is just around the corner!! Calling all procrastinators to register for the event. It's cheap, cheap, cheap compared to other CEU events. I spoke with Claudia Faulkenberry this morning. She will be speaking about the hot, hot antibody PDL1. (no, it ain't cheap ) It's on television commercials so the general public is asking about it. She will tell us the uses for this antibody as well as the various clones available. Companion diagnostics is the new buzz word for PDL1. Thank you Claudia. See you at Lake Lanier, Billie Zimmerman MT(ASCP)QIHC ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 160, Issue 15 ***************************************** From vkline650 at comcast.net Wed Mar 15 15:38:25 2017 From: vkline650 at comcast.net (Victoria Kline) Date: Wed, 15 Mar 2017 16:38:25 -0400 Subject: [Histonet] more MOHS questions Message-ID: <760302372180413DB6979CF910440762@vicPC> Hi fellow histotechs! Currently working on our procedure manual for MOHS and had a question about optimal room temperature and humidity. I know CLIA requires that we keep track of this, but I'm struggling to find information on what readings are optimal for MOHS. Our MOHS set up is in a very small room in a private derm center and I'll be honest, it gets rather warm and stuffy in there due to such a small space. I do keep the door open, and the blind shut when the sun is out, but we're on a AC track that services a few patient rooms and asking to drop the temp has resulted in some chilly patients. Also, any advice on controlling static. I'm having a lot of issues with tissue curling once I lift the anti roll plate. I've thought about getting an anti static brush, but wasn't sure what you all do. I don't know if keeping a dryer sheet tucked in the back will make a difference. I struggled so much this week with the anti roll plate. It worked beautifully on some cases and then there were condensing and tissue tearing on others. I'm assuming I really shouldn't need to adjust it for each case, but perhaps I am mistaken. Some of our edge is a bit chipped, so I think I really need to flip the glass. (which I've never done before, but I imagine its just loosening the two top screws, sliding the glass out and then tightening). We have a Leica and the manual states we should be able to use all 4 sides of the glass. Our eyewash station and microscope logs are done by the derm practice the MOHs lab takes place in. Do I still need to include the policy and do separate logs for CLIA for MOHS? Its a shared location. Most of the week its just for routine derm, and on Mondays we do MOHS there. Thanks in advance for all the help! Victoria From BZIMMERM at augusta.edu Thu Mar 16 10:18:51 2017 From: BZIMMERM at augusta.edu (Zimmerman, Billie) Date: Thu, 16 Mar 2017 15:18:51 +0000 Subject: [Histonet] GSH annual Histopalooza April 21st - April 23rd Message-ID: Come to Lake Lanier Islands for cheap, cheap CEUs! There's a lot of "bang for the buck" at our annual meeting. Yesterday, I mentioned Claudia Faulkenberry will be speaking about PDL1. This is the awesome antibody that's here to stay. Joe Meyers will be lecturing at Histopalooza. I've already told him to not do "death by PowerPoint". I'll just make sure he doesn't drink a 5 hour energy drink. His topic will be : "Similarities and Differences Between Immunostaining for Routine Assessment and Treatment Decision-Making". Joe will address companion/complementary diagnostics, and in particular PD1/PD-L1 testing. I've looking forward to learning more about these antibodies. Companion Diagnostics is the buzz phrase for IHC Looking forward to seeing y'all soon. Bring the family-there's something for everyone. Or, come by yourself (why take sand to the beach?) Billie Zimmerman MT(ASCP)QIHC GSH PR From relia1 at earthlink.net Thu Mar 16 10:57:27 2017 From: relia1 at earthlink.net (Pam Barker) Date: Thu, 16 Mar 2017 11:57:27 -0400 Subject: [Histonet] Histology Opportunities in Florida!!! New Grads Welcome!!! Message-ID: <05e701d29e6e$0f8792a0$2e96b7e0$@earthlink.net> Hi Histonetters! How are you? I have several new histology positions in Florida!! And I need your help. I am currently working with a couple of my best clients who are private dermatology labs in FL that are in need of a histo tech and a Mohs Tech. These are permanent full time dayshift positions. My clients offer great environments, great crews to work with the opportunity to learn new things like Mohs! And excellent salary and benefits. Florida license is required. Here are the specifics for each position: Histotech - process permanent and Mohs sections - full time dayshift in house lab ASCP HT and Florida license required. Opportunity to Learn MOHS!!! New GRADS WELCOME TO APPLY Mohs Tech - Full time Mohs tech floating between several doctors in the local area. Must be proficient in Mohs. My question is do you know of anyone who might be interested in these positions? I really appreciate you taking the time to read this e-mail and it means a lot to me when you take the time to refer your friends and coworkers. So if you think you or someone you know might be interested please contact me. Don't forget if I place someone you refer you will earn a referral fee!! I can be reached at 866-607-3542 or cell/text 407-353-5070 or relia1 at earthlink.net Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From tgenade at gmail.com Thu Mar 16 12:36:10 2017 From: tgenade at gmail.com (Tyrone Genade) Date: Thu, 16 Mar 2017 12:36:10 -0500 Subject: [Histonet] FYI: FastRed vs 88% formic acid Message-ID: Hello, Sticking with the theme... In case you are curious Abcam's FastRed ( http://www.abcam.com/liquid-fast-red-substrate-kit-ab64254.html) on IHC sections is resistant to 88% formic acid. There is a little fading but it holds up well. Bye -- Tyrone Genade Orange City, Iowa tel: (+1) 712 230 4101 http://tgenade.freeshell.org ******************************************************************************** Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord. To find out how to receive this FREE gift visit http://www.alpha.org. From Rosalie.Agrow at piedmont.org Thu Mar 16 13:47:37 2017 From: Rosalie.Agrow at piedmont.org (Rosalie N. Agrow) Date: Thu, 16 Mar 2017 18:47:37 +0000 Subject: [Histonet] Tek SmartWrite Message-ID: <202e5a9c6ede42b1b8806688c40768d7@PHCMM211.piedmonthospital.org> Our histology lab purchased the Tissue-Tek SmartWrite cassette and slide printers. We're in the process of interfacing the cassette printer with the hope that the barcode on the cassette will drive the slide printer to print the exact # of slides necessary for each case. If any of you have this equipment and successfully implemented this process please contact me as I would love to talk to you. Thank you. Rosalie Agrow, B.S., CT(ASCP) | Manager | Anatomic Pathology Laboratory | 1968 Peachtree Road | 77 Building, 4th Floor | Atlanta, GA 30309 O: 404-605-2142 |F: 404-609-6645 |E: Rosalie.Agrow at piedmont.org If your messaging client supports TLS for secure connections, then this message was sent securely via TLS from Piedmont Healthcare, Inc. This e-mail communication, including any attached files may contain material that is proprietary, privileged, confidential, or otherwise legally exempt from disclosure. This communication is intended solely for the use of the individual or entity to which it is addressed. If you are not the intended recipient or the person responsible for delivering this communication to the intended recipient, you are prohibited from retaining, using, disseminating, forwarding, printing or copying this communication. If you have received this communication in error, please immediately notify the sender via return e-mail or telephone. This email has been scanned and found to be virus free. If this message contains a virus please contact postmaster at piedmont.org. This original email was sent to the internet for delivery at 14:47:35 From aaditza1 at gmail.com Fri Mar 17 10:39:04 2017 From: aaditza1 at gmail.com (Aditza) Date: Fri, 17 Mar 2017 10:39:04 -0500 Subject: [Histonet] -80c freezer, blood safe at -65c too? Message-ID: Dear all, In a research lab how much the -80c freezer can drop the temperature and the integrity of the blood, serum or tissue will not be affected? -65c is still good? Or -50c? Sincerely, Adriana Rosca BS, HTL (ASCP) MS "...what we say and how we say it, is still the foundation of behavior change." From tejohnson at genoptix.com Sat Mar 18 13:43:05 2017 From: tejohnson at genoptix.com (Teri Johnson) Date: Sat, 18 Mar 2017 18:43:05 +0000 Subject: [Histonet] So long and thanks for all the fish! Message-ID: Dr. Richmond, Thank you for all your contributions to the histonet over the years. I would have loved working with you. I know I loved learning from you. Best wishes and happy retirement! Teri Johnson, HT(ASCP)QIHC Manager, Clinical Trial Testing T +1 760 516 5954 tejohnson at genoptix.com Navigate BioPharma, Inc. A Novartis Company 1890 Rutherford Rd. Carlsbad, CA 92008 USA ________________________________ CONFIDENTIALITY NOTICE: The sender of this email is not an employee or agent of, or a contractor or consultant to, Genoptix. This email has been sent through the Genoptix email system as a transitional service provided by Genoptix, Inc. ("Genoptix") to Navigate BioPharma Services, Inc. The sender of this email is not authorized to represent or to speak on behalf of any member of Genoptix, nor to enter into any undertaking, arrangement or agreement on behalf of any member of Genoptix. Any such undertaking, arrangement or agreement shall not be binding on Genoptix. Genoptix accepts no liability for the content of this email, or for the consequences of any acts or omissions taken or not taken on the basis of the information contained in this email or in any email sent from this email address. All reasonable precautions have been taken to ensure no viruses or other malware are present in this e-mail and Genoptix cannot accept responsibility for any loss or damage arising from the use of this e-mail or attachments and you are responsible for ensuring that your own virus screening procedures are up to date and fit for purpose. From mbmphoto at gmail.com Sun Mar 19 19:10:41 2017 From: mbmphoto at gmail.com (Maria Mejia) Date: Sun, 19 Mar 2017 17:10:41 -0700 Subject: [Histonet] need help staining 120um human whole brain sections! In-Reply-To: <761E2B5697F795489C8710BCC72141FF8E5C19DF@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF8E5C19DF@ex07.net.ucsf.edu> Message-ID: <2F3406BA-CAB9-487D-9D62-FCB7EF461CDC@gmail.com> Or lab is currently processing a human whole brain. In about a month or two, the whole brain, which will be encased in celloidin & serial sections will be cut at 120um each. Now, we?ve bought an old Tetrander cast iron microtome. If you haven?t seen one of these microtomes, I can tell you it?s BIG! Now, we?ll have to stain quite a number of these sections for IHC. In fact too many to handle manually. If possible, need to find a way to at least stain the majority of these sections in a semi-automatic system e.g. washes, quenching & blocking. Does any one think it?s possible to convert a LABGO processor (made in India & can hold 2 liter glass beakers) or a Sakura-Tissue-Tek 4640 (also holds glass beakers - both are old style circular processors using glass beakers for staining these sections? Does anyone have an alternative system? I could sure use some input or ideas anyone welcome and most appreciated. Maria Mejia Lead Histologist UCSF Mission Bay San Francisco, CA From rjbuesa at yahoo.com Mon Mar 20 08:51:12 2017 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Mon, 20 Mar 2017 13:51:12 +0000 (UTC) Subject: [Histonet] need help staining 120um human whole brain sections! In-Reply-To: <2F3406BA-CAB9-487D-9D62-FCB7EF461CDC@gmail.com> References: <761E2B5697F795489C8710BCC72141FF8E5C19DF@ex07.net.ucsf.edu> <2F3406BA-CAB9-487D-9D62-FCB7EF461CDC@gmail.com> Message-ID: <745821795.3803443.1490017872520@mail.yahoo.com> You have a special project ? special tasks so your approach has to be equally special.Large brain sections are usually stained while floating but for IH with different and successive steps requiring very expensive reagents, floating sections is not well suited.You should affix the sections to large slides, and I imagine will not be brain whole sections but limited to some areas.In that case for IHC you can stain several sections in a humid chamber, manually.?I do not imagine an automatic system for this task.It will be a costly and slow process indeed.Ren? On Sunday, March 19, 2017 8:27 PM, Maria Mejia via Histonet wrote: Or lab is currently processing a human whole brain.? In about a month or two, the whole brain, which will be encased in celloidin & serial sections will be cut at 120um each.? Now, we?ve bought an old Tetrander cast iron microtome. If you haven?t seen one of these microtomes, I can tell you it?s BIG! Now, we?ll have to stain quite a number of these sections for IHC.? In fact too many to handle manually. If possible, need to find a way to at least stain the majority of these sections in a semi-automatic system e.g. washes, quenching & blocking. Does any one think it?s possible to convert a LABGO processor (made in India & can hold 2 liter glass beakers) or a Sakura-Tissue-Tek 4640 (also holds glass beakers - both are old style circular processors using glass beakers for staining these sections? Does anyone have an alternative system? I could sure use some input or ideas anyone welcome and most appreciated. Maria Mejia Lead Histologist UCSF Mission Bay San Francisco, CA _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jackie.OConnor at abbvie.com Mon Mar 20 09:07:55 2017 From: Jackie.OConnor at abbvie.com (Oconnor, Jackie M) Date: Mon, 20 Mar 2017 14:07:55 +0000 Subject: [Histonet] Toxicology Histology Supervisor Position Message-ID: Hello Histonetters - we are looking for a Histology Supervisor. If you are interested, please email me. Jackie From mbmphoto at gmail.com Mon Mar 20 10:46:53 2017 From: mbmphoto at gmail.com (Maria Mejia) Date: Mon, 20 Mar 2017 08:46:53 -0700 Subject: [Histonet] need help staining 120um human whole brain sections! In-Reply-To: <745821795.3803443.1490017872520@mail.yahoo.com> References: <761E2B5697F795489C8710BCC72141FF8E5C19DF@ex07.net.ucsf.edu> <2F3406BA-CAB9-487D-9D62-FCB7EF461CDC@gmail.com> <745821795.3803443.1490017872520@mail.yahoo.com> Message-ID: <1B42B140-10BE-46AA-B5C6-CEBAA6036DC0@gmail.com> Good morning Rene, I have worked out special staining IHC protocols to work on celloidin cut sections from 60um to 600um thick. These are free-floating human whole brainstem & whole brain sections. The IHC is amazing using single, double & triple. We save on antibodies, ABC, polymer, TSA using the parafilm method. For example: 1) I use a sheet of parafilm slightly larger than the section to be stained & it?s placed on flat surface (glass slide or dish or whatever). 2) Using a pipette, small drops of working diluted antibody are placed on the parafilm surface to match the size of the tissue section (from 500ul to 1ml). 3) Then the tissue section is free-floated from PBST/2% Triton onto another cut sheet of parafilm, where carefully the section is floating onto the middle surface of the antibody drops by using a brush. As the section lays flat, the drops spread throughout the tissue surface - evenly covering the bottom of the tissue section. 4) This step is repeated for the top surface & the section is gently sealed by placing another parafilm sheet on the top surface of the section to spread the antibody & seal the section for incubation overnight. This method works, although time consuming! The problem is for the washes, quenching & blocking - we need a semi automatic IHC system to take of these steps. To bad there isn?t a robotic arm we could have built & programed to do the above steps. We need an inventor type person to build what I can imagine. best Maria > On Mar 20, 2017, at 6:51 AM, Rene J Buesa wrote: > > You have a special project ? special tasks so your approach has to be equally special. > Large brain sections are usually stained while floating but for IH with different and successive steps requiring very expensive reagents, floating sections is not well suited. > You should affix the sections to large slides, and I imagine will not be brain whole sections but limited to some areas. > In that case for IHC you can stain several sections in a humid chamber, manually. I do not imagine an automatic system for this task. > It will be a costly and slow process indeed. > Ren? > > > On Sunday, March 19, 2017 8:27 PM, Maria Mejia via Histonet wrote: > > > > Or lab is currently processing a human whole brain. In about a month or two, the whole brain, which will be encased > in celloidin & serial sections will be cut at 120um each. Now, we?ve bought an old Tetrander cast iron microtome. If > you haven?t seen one of these microtomes, I can tell you it?s BIG! > > Now, we?ll have to stain quite a number of these sections for IHC. In fact too many to handle manually. If possible, need > to find a way to at least stain the majority of these sections in a semi-automatic system e.g. washes, quenching & blocking. > > Does any one think it?s possible to convert a LABGO processor (made in India & can hold 2 liter glass beakers) or a > Sakura-Tissue-Tek 4640 (also holds glass beakers - both are old style circular processors using glass beakers for staining > these sections? Does anyone have an alternative system? I could sure use some input or ideas anyone welcome > and most appreciated. > > Maria Mejia > Lead Histologist > UCSF > Mission Bay > San Francisco, CA > > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From KSimeone at leavittmgt.com Mon Mar 20 13:26:31 2017 From: KSimeone at leavittmgt.com (Kari Simeone) Date: Mon, 20 Mar 2017 18:26:31 +0000 Subject: [Histonet] FT EVENING HT POSTITION Delray Beach, FL Message-ID: <43944B1DBAAC2846B7B9D626B5F1233CC91BE61E@vm-email.leavittmgt.com> Hi Histonetters! We are looking for a full time licensed HISTOTECHNOLOGIST here in our very busy Delray Beach Florida Dermatology Lab. This is a permanent full time EVENING SHIFT (40 hours) position with benefits (medical/401k/vacation). THIS IS A DRUG FREE WORKPLACE. Background check, personality test and drug test will be necessary. Sorry, no relocation assistance provided. ***PLEASE NO HEAD HUNTERS/PLACEMENT SERVICES***!!! Please fill out employment application HERE https://www.indeed.com/viewjob?jk=ec6f15bf3fbbbdf7&q=histotechnologist&l=florida&tk=1bbmf648ua3cqdc2&from=web ^^you MUST follow this application link to apply! No exceptions. *FULL TIME position Mon-Fri OR Sun-Thurs 5P-1:30AM (START TIME CAN VARY FROM 5-10P) *MUST be licensed as a FL HISTOTECHNOLOGIST ONLY (will be working solo some of your shift) *MUST have at LEAST FIVE (5) years experience (dermatology preferred) Please DO NOT respond if no EXPERIENCE! *VERY proficient in embedding and microtomy *WILL MOSTLY BE EMBEDDING EXCISION BLOCKS, please know DERMS *must be self motivated, reliable and a team player *knowledge in operating Ventana and Leica equipment desired (not necessary) *some IHC experience preferred but not necessary Kari M Simeone The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. From lguernsey at ucsd.edu Mon Mar 20 15:53:29 2017 From: lguernsey at ucsd.edu (Lucie Guernsey) Date: Mon, 20 Mar 2017 13:53:29 -0700 Subject: [Histonet] Use of sodium borohydride in immunofluorescence Message-ID: Hi, I'm currently going over IHC SOPs that were written by lab members that have long ago moved on to other jobs. One inconsistency that I've come across is the use of sodium borohydride in immunofluorescence. Our protocols state that a '0.5% sodium borohydride solution should be used to block aldehyde fluorescence if staining CNS tissue in paraffin sections' (this step is not included in our cryosection protocol). Is there a particular reason that it should only be done in CNS tissue? Seems to me that all tissues, regardless of type, should benefit from blocking aldehyde fluorescence as the problem is caused by the fixative, not the tissue type. Which leads me to the follow up question of: Our cryosection protocol should also include sodium borohydride blocking if the tissues were at any point fixed using an aldehyde fixative, correct? There's a chance I have pregnancy brain and an obvious answer is slipping past me, but my current opinion is that any and all immunofluorescence done on paraformaldehyde (our usual fixative) fixed tissue should undergo sodium borohydride blocking. Am I missing something? Thanks for any help in clarifying this! Lucie Lucie Guernsey UC San Diego lguernsey at ucsd.edu From relia1 at earthlink.net Tue Mar 21 10:31:25 2017 From: relia1 at earthlink.net (Pam Barker) Date: Tue, 21 Mar 2017 11:31:25 -0400 Subject: [Histonet] Spring Break Staycation Ideas!! And Some Exciting Histology Opportunities! Message-ID: <09ea01d2a258$34bf74f0$9e3e5ed0$@earthlink.net> Hi Histonetters, How are you? I hope you are having a great week! Can you believe it's Spring Break already? Since I live in Florida we see spring breakers from all over the U.S. during the months of March and April. Not everyone jets away to an "exotic locale" for Spring Break and. I saw something the other day and thought I would share it with you. If you are looking for fun things to do in your area during Spring Break try this: Google "Spring Break Staycation and the name of your city" You will find tons of fun stuff to do right there in your own backyard! Whatever you do for Spring Break - Have Fun, Be Safe and Wear Sunscreen!! I also wanted to give you a heads up on my current open positions. These are exciting opportunities with some of the finest employers in the country. Management Opportunities: Lab Manager - Danville, VA Histology Lab Manager - Los Angeles, CA Histology Lab Manager - San Diego, CA Histology Opportunities Histotechnician - Austin, TX - Great Crew to work with!! Histotechnician - Modesto, CA - Brand New Lab!!! IHC tech - Modesto, CA - Brand New Lab Dermpath Histotech - Longview, TX - Learn Mohs!! Histotech - San Diego, CA - Brand new Lab!! Histology Tech - Ft. Myers, FL - Learn Mohs!! Histotech - Wilmington, NC - CLIA qualified to Gross. Histotech - Cranford, NJ - Very Nice Environment!!! *If you happen to know any junior techs or recent grads that are ASCP certified many of my clients are interested in speaking with them as well! All of my clients offer excellent compensation, benefits and some offer relocation assistance and or sign on bonuses. All of these jobs are full time & permanent & most of them are RELIA Exclusives!!! If you or anyone you know is interested in hearing more about any of these opportunities please contact me. Remember if I place someone you refer to me you will earn a referral bonus. I can be reached ASAP on my cell/text at 407-353-5070 or e-mail me at relia1 at earthlink.net Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From skiousis at med.wayne.edu Tue Mar 21 10:53:04 2017 From: skiousis at med.wayne.edu (Sam Kiousis) Date: Tue, 21 Mar 2017 15:53:04 +0000 Subject: [Histonet] (no subject) Message-ID: Does anyone know the histo standard antibodies for differentiating sarcoma from glial components in a gliasarcoma cell line Ive been growing? Any help would be appreciated Thanks Sam Kiousis From BZIMMERM at augusta.edu Tue Mar 21 12:38:24 2017 From: BZIMMERM at augusta.edu (Zimmerman, Billie) Date: Tue, 21 Mar 2017 17:38:24 +0000 Subject: [Histonet] GSH Annual Histopalooza April 21 - April 23` Message-ID: Just a friendly reminder about the upcoming meeting at Legacy Lodge, Lake Lanier Islands Here's the link for registration: http://www.histosearch.com/gsh/2017GSHReg.pdf Featured speakers: Ely Klar, Joe Meyers, Tonia Crook, Danielle Boleska, and Tania Nigro Check out the program online. You'll see it's a lot of bang for the buck! Also please come and support our vendors, too. Billie Zimmerman MT(ASCP) PR for GSH From Evelyn.Flynn at childrens.harvard.edu Tue Mar 21 14:29:17 2017 From: Evelyn.Flynn at childrens.harvard.edu (Flynn, Evelyn) Date: Tue, 21 Mar 2017 19:29:17 +0000 Subject: [Histonet] Embedding station Message-ID: <1490124557640.62398@childrens.harvard.edu> Hello all, Our laboratory is purchasing a new paraffin embedding station. We are considering a Leica Arcadia or a Thermo HistoStar model. Has anyone had good or bad experiences with either of these? Thanks for your input, Evelyn Flynn Lead Research Technologist Boston Children's Hospital From rsrichmond at gmail.com Wed Mar 22 09:12:27 2017 From: rsrichmond at gmail.com (Bob Richmond) Date: Wed, 22 Mar 2017 10:12:27 -0400 Subject: [Histonet] Use of sodium borohydride in immunofluorescence Message-ID: > > Lucie Guernsey at UC San Diego asks about sodium borohydride: >>I'm currently going over IHC SOPs that were written by lab members that have long ago moved on to other jobs. One inconsistency that I've come across is the use of 0.5 % sodium borohydride in immunofluorescence.<< Does your protocol include the information that sodium borohydride is a serious hazmat? Here's the Sigma-Aldrich MSDS: http://www.sigmaaldrich.com/catalog/product/sigma/s9125?lang=en®ion=US >>There's a chance I have pregnancy brain....<< Best wishes to you and your child! Bob Richmond Samurai Pathologist Maryville TN > > From rjbuesa at yahoo.com Wed Mar 22 09:40:39 2017 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Wed, 22 Mar 2017 14:40:39 +0000 (UTC) Subject: [Histonet] Embedding station In-Reply-To: <1490124557640.62398@childrens.harvard.edu> References: <1490124557640.62398@childrens.harvard.edu> Message-ID: <462485727.596443.1490193639882@mail.yahoo.com> "Open" your options?and try Sakura.Ren? On Tuesday, March 21, 2017 3:54 PM, "Flynn, Evelyn via Histonet" wrote: Hello all, ? Our laboratory is purchasing a new paraffin embedding station.? We are considering a Leica Arcadia or a Thermo HistoStar model. ? Has anyone had good or bad experiences with either of these? Thanks for your input, Evelyn Flynn Lead Research Technologist Boston Children's Hospital _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From GauchV at mail.amc.edu Wed Mar 22 09:53:45 2017 From: GauchV at mail.amc.edu (Gauch, Vicki) Date: Wed, 22 Mar 2017 14:53:45 +0000 Subject: [Histonet] Ventana HE 600 Message-ID: Hi, Is anyone out there using the Ventana HE 600 stainer in their lab? If so, I would be very interested in hearing the pros and cons. Thanks so much, Vicki AMCH Dept. of Pathology Albany, NY ----------------------------------------- CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. From BHaines at caperegional.com Wed Mar 22 10:11:38 2017 From: BHaines at caperegional.com (Haines, Beth) Date: Wed, 22 Mar 2017 15:11:38 +0000 Subject: [Histonet] IHC billing on archived case Message-ID: <43A12C6F0219D24F8C5041DE2D7FC85702861895A8@CRMCEXSRV01.BTHOSP.INT> Hello all, After some discussion on IHC billing, I have been asked to verify accepted billing practice for the following situation: An IHC request for 2 single antibody stains has been made by outpatient oncology practice on a case (same block) that was completed nearly a year ago. 6 IHCs were done initially (billed as 88342 & 88341 x 5). How should the new request be billed? Should a new visit/encounter be created and 88342 & 88341 be billed for this encounter? Thanks in advance for the guidance. Beth Haines BS, HTL (ASCP) bhaines at caperegional.com Histology Supervisor Cape Regional Medical Center Confidentiality Notice: This e-mail message, including any attachments, from Cape Regional Health System contains information which is CONFIDENTIAL AND/OR LEGALLY PRIVILEGED.The information is intended only for the use of the individual named above and may not be disseminated to any other party without Cape Regional Health System's written permission. If you are not the intended recipient, or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, disclosure, distribution, copying or taking of any action in reliance on the contents of this e-mailed information is strictly prohibited. If you have received this e-mail in error, please notify us immediately by telephone at 609-463-2163 to arrange for the return of these documents to us without cost to you. From rjbuesa at yahoo.com Wed Mar 22 11:27:38 2017 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Wed, 22 Mar 2017 16:27:38 +0000 (UTC) Subject: [Histonet] IHC billing on archived case In-Reply-To: <43A12C6F0219D24F8C5041DE2D7FC85702861895A8@CRMCEXSRV01.BTHOSP.INT> References: <43A12C6F0219D24F8C5041DE2D7FC85702861895A8@CRMCEXSRV01.BTHOSP.INT> Message-ID: <493815964.1422901.1490200058624@mail.yahoo.com> You should treat it as a new request.Ren? On Wednesday, March 22, 2017 11:25 AM, "Haines, Beth via Histonet" wrote: Hello all, After some discussion on IHC billing, I have been asked to verify accepted billing practice for the following situation: An IHC request for 2 single antibody stains has been made by outpatient oncology practice on a case (same block) that was completed nearly a year ago. 6 IHCs were done initially (billed as 88342 & 88341 x 5). How should the new request be billed? Should a new visit/encounter be created and 88342 & 88341 be billed for this encounter? Thanks in advance for the guidance. Beth Haines BS, HTL (ASCP) bhaines at caperegional.com Histology Supervisor Cape Regional Medical Center Confidentiality Notice: This e-mail message, including any attachments, from Cape Regional Health System contains information which is CONFIDENTIAL AND/OR LEGALLY PRIVILEGED.The information is intended only for the use of the individual named above and may not be disseminated to any other party without Cape Regional Health System's written permission. If you are not the intended recipient, or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, disclosure, distribution, copying or taking of any action in reliance on the contents of this e-mailed information is strictly prohibited.? If you have received this e-mail in error, please notify us immediately by telephone at 609-463-2163 to arrange for the return of these documents to us without cost to you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joyce.weems at emoryhealthcare.org Wed Mar 22 11:31:56 2017 From: joyce.weems at emoryhealthcare.org (Weems, Joyce K.) Date: Wed, 22 Mar 2017 16:31:56 +0000 Subject: [Histonet] IHC billing on archived case In-Reply-To: <493815964.1422901.1490200058624@mail.yahoo.com> References: <43A12C6F0219D24F8C5041DE2D7FC85702861895A8@CRMCEXSRV01.BTHOSP.INT> <493815964.1422901.1490200058624@mail.yahoo.com> Message-ID: However, if it is a Medicare Patient - it would now be an archived case and would be treated as non-hospital. Medicare should pay. j Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax 770-380-8099 Cell joyce.weems at emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph?s Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: Rene J Buesa via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, March 22, 2017 12:28 PM To: Haines, Beth ; 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] IHC billing on archived case You should treat it as a new request.Ren? On Wednesday, March 22, 2017 11:25 AM, "Haines, Beth via Histonet" wrote: Hello all, After some discussion on IHC billing, I have been asked to verify accepted billing practice for the following situation: An IHC request for 2 single antibody stains has been made by outpatient oncology practice on a case (same block) that was completed nearly a year ago. 6 IHCs were done initially (billed as 88342 & 88341 x 5). How should the new request be billed? Should a new visit/encounter be created and 88342 & 88341 be billed for this encounter? Thanks in advance for the guidance. Beth Haines BS, HTL (ASCP) bhaines at caperegional.com Histology Supervisor Cape Regional Medical Center Confidentiality Notice: This e-mail message, including any attachments, from Cape Regional Health System contains information which is CONFIDENTIAL AND/OR LEGALLY PRIVILEGED.The information is intended only for the use of the individual named above and may not be disseminated to any other party without Cape Regional Health System's written permission. If you are not the intended recipient, or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, disclosure, distribution, copying or taking of any action in reliance on the contents of this e-mailed information is strictly prohibited. If you have received this e-mail in error, please notify us immediately by telephone at 609-463-2163 to arrange for the return of these documents to us without cost to you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From murphyv at karmanos.org Wed Mar 22 11:32:42 2017 From: murphyv at karmanos.org (Murphy, Valerie) Date: Wed, 22 Mar 2017 16:32:42 +0000 Subject: [Histonet] Slides for Tissue microarrays Message-ID: Does anyone make their own sticky slides for cutting TMA blocks? Is there an adhesive substance we could apply to our regular plus slides? Valerie Ratliff BS HT(ASCP) Wayne State University McLaren confidentiality statement: "The information contained in this communication, including attachments, is confidential, may be privileged, and is intended only for the use of the named recipient(s). Unauthorized use, disclosure, forwarding or copying is strictly prohibited and may be unlawful. If you have received this communication in error, please notify me IMMEDIATELY at the phone number or pager listed above." From craigak12 at gmail.com Wed Mar 22 11:55:24 2017 From: craigak12 at gmail.com (J B) Date: Wed, 22 Mar 2017 16:55:24 +0000 Subject: [Histonet] Retic Kit: Message-ID: Can anyone recommend a good reticulum kit to use? We currently are using a hand stain kit from Stat Lab & can not get rid of the precipitate. Any suggestions are greatly appreciated. Sincerely, JB -- Have a great day! From hardin at oncology.wisc.edu Wed Mar 22 11:57:59 2017 From: hardin at oncology.wisc.edu (Joseph Hardin) Date: Wed, 22 Mar 2017 16:57:59 +0000 Subject: [Histonet] HE autostainer Message-ID: I am looking for a small H+E autostainer to be used in a research core histo lab. We run from 10 to 150 H+Es a day. Any suggestions? Joseph Hardin Senior Research Specialist UWCCC Experimental Pathology WIMR I Rm. 4012 1111 Highland Av. Madison, WI 53705 (608)262-1836 From pablo.sanchez at usc.es Wed Mar 22 12:38:37 2017 From: pablo.sanchez at usc.es (=?UTF-8?Q?Pablo_S=c3=a1nchez_Quinteiro?=) Date: Wed, 22 Mar 2017 18:38:37 +0100 Subject: [Histonet] Sections falling off Message-ID: <3e496e32-7735-e770-5bdc-1fa8925879f6@usc.es> Dear Histonetters, I am in trouble with my paraffin sections -samples fixed in Bouin's liquid. Just after the first steps: Two xylol baths and 100% ethanol most of them are falling off. Slides are silanised. I do not think it was an adhesion problem. What do you think about? Embedding, cuttibg? Thanks a lot in advance Pablo --- El software de antivirus Avast ha analizado este correo electr?nico en busca de virus. https://www.avast.com/antivirus From philip_manfre at merck.com Wed Mar 22 12:53:14 2017 From: philip_manfre at merck.com (Manfre, Philip) Date: Wed, 22 Mar 2017 17:53:14 +0000 Subject: [Histonet] Sections falling off In-Reply-To: <3e496e32-7735-e770-5bdc-1fa8925879f6@usc.es> References: <3e496e32-7735-e770-5bdc-1fa8925879f6@usc.es> Message-ID: -----Original Message----- From: Pablo S?nchez Quinteiro via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, March 22, 2017 1:39 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Sections falling off Dear Histonetters, I am in trouble with my paraffin sections -samples fixed in Bouin's liquid. Pablo, I think your sections are not completely dry, after placing them on slides. Silanized slides can trap water between the sections and the slide, almost appearing like a bulge. You may need to use the corner of a paper towel to "poke" the paraffin section and drain off the excess water. Make sure the sections look dry before starting the staining procedure. They should be transparent (translucent), not opaque (milky white). That's my guess Phil. Philip Manfre, HT (ASCP) Associate Principal Scientist Merck Research Laboratories WP81-406 770 Sumneytown Pike West Point, PA 19486 215-652-9750 215-993-0383 (fax) philip_manfre at merck.com Just after the first steps: Two xylol baths and 100% ethanol most of them are falling off. Slides are silanised. I do not think it was an adhesion problem. What do you think about? Embedding, cuttibg? Thanks a lot in advance Pablo --- El software de antivirus Avast ha analizado este correo electr?nico en busca de virus. https://www.avast.com/antivirus _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From pablo.sanchez at usc.es Wed Mar 22 13:19:08 2017 From: pablo.sanchez at usc.es (=?UTF-8?Q?Pablo_S=c3=a1nchez_Quinteiro?=) Date: Wed, 22 Mar 2017 19:19:08 +0100 Subject: [Histonet] Sections falling off In-Reply-To: <030001d2a333$d2cb63d0$78622b70$@imebinc.com> References: <3e496e32-7735-e770-5bdc-1fa8925879f6@usc.es> <030001d2a333$d2cb63d0$78622b70$@imebinc.com> Message-ID: <7798f2cc-4719-3962-9bd4-8620e537a0c7@usc.es> Thanks for your hint Travis. The slides are new and good. Dako Flex Immuno Slides. Pablo El 22/03/2017 a las 18:43, Travis O'Brien escribi?: > Could be bad or expired Slides! What type are you using? > > > Travis O?Brien > > International Medical Equipment, Inc. > 170 Vallecitos De Oro > San Marcos, CA 92069 > > 800.543.8496 Phone > 760.761.0859 Fax > www.imebinc.com > > > > > -----Original Message----- > From: Pablo S?nchez Quinteiro via Histonet [mailto:histonet at lists.utsouthwestern.edu] > Sent: Wednesday, March 22, 2017 10:39 AM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Sections falling off > > Dear Histonetters, > > I am in trouble with my paraffin sections -samples fixed in Bouin's liquid. > > Just after the first steps: Two xylol baths and 100% ethanol most of them are falling off. Slides are silanised. > > I do not think it was an adhesion problem. What do you think about? > Embedding, cuttibg? > > Thanks a lot in advance > > Pablo > > > --- > El software de antivirus Avast ha analizado este correo electr?nico en busca de virus. > https://www.avast.com/antivirus > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > . > --- El software de antivirus Avast ha analizado este correo electr?nico en busca de virus. https://www.avast.com/antivirus From pablo.sanchez at usc.es Wed Mar 22 13:22:13 2017 From: pablo.sanchez at usc.es (=?UTF-8?Q?Pablo_S=c3=a1nchez_Quinteiro?=) Date: Wed, 22 Mar 2017 19:22:13 +0100 Subject: [Histonet] Sections falling off In-Reply-To: <219fa61b472c4c30a8eedff28237dcd2@spiderman.MercerU.local> References: <3e496e32-7735-e770-5bdc-1fa8925879f6@usc.es> <219fa61b472c4c30a8eedff28237dcd2@spiderman.MercerU.local> Message-ID: <99d89ba4-75ad-5187-c4ed-fff5a913bddc@usc.es> Thanks for your reply Shirley. After 24 h fixation in Bouin samples are passed into 70? ethanol. I have tried drying the sections at RT for short or long period (30?) and then 37? oven overnight. The tissue is mouse brain, sections thickness 8 microns. Pablo El 22/03/2017 a las 18:45, Shirley A. Powell escribi?: > Are you washing the bouins out of the tissue well before processing, that may be one source. Also how long are you drying your sections before staining? What type of tissue are you processing? > > -----Original Message----- > From: Pablo S?nchez Quinteiro via Histonet [mailto:histonet at lists.utsouthwestern.edu] > Sent: Wednesday, March 22, 2017 1:39 PM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Sections falling off > > Dear Histonetters, > > I am in trouble with my paraffin sections -samples fixed in Bouin's liquid. > > Just after the first steps: Two xylol baths and 100% ethanol most of them are falling off. Slides are silanised. > > I do not think it was an adhesion problem. What do you think about? > Embedding, cuttibg? > > Thanks a lot in advance > > Pablo > > > --- > El software de antivirus Avast ha analizado este correo electr?nico en busca de virus. > https://www.avast.com/antivirus > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet --- El software de antivirus Avast ha analizado este correo electr?nico en busca de virus. https://www.avast.com/antivirus From pablo.sanchez at usc.es Wed Mar 22 13:24:09 2017 From: pablo.sanchez at usc.es (=?UTF-8?Q?Pablo_S=c3=a1nchez_Quinteiro?=) Date: Wed, 22 Mar 2017 19:24:09 +0100 Subject: [Histonet] Sections falling off In-Reply-To: <85f2fa54543c43f6b35e94822e2b1ae9@cdc.gov> References: <3e496e32-7735-e770-5bdc-1fa8925879f6@usc.es> <85f2fa54543c43f6b35e94822e2b1ae9@cdc.gov> Message-ID: Thanks for your reply Jeanine, Sections are 8 microns thick (mouse brain) and they are stored overnight at 37?C Pablo El 22/03/2017 a las 18:51, Sanders, Jeanine (CDC/OID/NCEZID) escribi?: > Hi Pablo. > > How thick are your sections and how long and at what temperature do you heat the slides before beginning de-paraffinizing? > > Jeanine H. Sanders > Infectious Diseases Pathology Branch > Centers for Disease Control and Prevention > 1600 Clifton Rd., NE MS-G32 > Atlanta, GA 30329 > > -----Original Message----- > From: Pablo S?nchez Quinteiro via Histonet [mailto:histonet at lists.utsouthwestern.edu] > Sent: Wednesday, March 22, 2017 1:39 PM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Sections falling off > > Dear Histonetters, > > I am in trouble with my paraffin sections -samples fixed in Bouin's liquid. > > Just after the first steps: Two xylol baths and 100% ethanol most of them are falling off. Slides are silanised. > > I do not think it was an adhesion problem. What do you think about? > Embedding, cuttibg? > > Thanks a lot in advance > > Pablo > > > --- > El software de antivirus Avast ha analizado este correo electr?nico en busca de virus. > https://www.avast.com/antivirus > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet --- El software de antivirus Avast ha analizado este correo electr?nico en busca de virus. https://www.avast.com/antivirus From pablo.sanchez at usc.es Wed Mar 22 13:29:03 2017 From: pablo.sanchez at usc.es (=?UTF-8?Q?Pablo_S=c3=a1nchez_Quinteiro?=) Date: Wed, 22 Mar 2017 19:29:03 +0100 Subject: [Histonet] Sections falling off In-Reply-To: References: <3e496e32-7735-e770-5bdc-1fa8925879f6@usc.es> Message-ID: <4f9a26a3-fa0d-b5f9-c4d0-86a0bd30417b@usc.es> Thanks for your hint Philip, They are stored at 37? overnight. I learnt the problem with the trapped water. I am very careful with it but they keep spoiling. They seem transparent. Could I try 60 minutes at 60? oven just before xylen? Pablo El 22/03/2017 a las 18:53, Manfre, Philip escribi?: > > -----Original Message----- > From: Pablo S?nchez Quinteiro via Histonet [mailto:histonet at lists.utsouthwestern.edu] > Sent: Wednesday, March 22, 2017 1:39 PM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Sections falling off > > Dear Histonetters, > > I am in trouble with my paraffin sections -samples fixed in Bouin's liquid. > > Pablo, > I think your sections are not completely dry, after placing them on slides. Silanized slides can trap water between the sections and the slide, almost appearing like a bulge. You may need to use the corner of a paper towel to "poke" the paraffin section and drain off the excess water. Make sure the sections look dry before starting the staining procedure. They should be transparent (translucent), not opaque (milky white). > That's my guess > > Phil. > > Philip Manfre, HT (ASCP) > Associate Principal Scientist > Merck Research Laboratories > WP81-406 > 770 Sumneytown Pike > West Point, PA 19486 > > 215-652-9750 > 215-993-0383 (fax) > philip_manfre at merck.com > > > > > Just after the first steps: Two xylol baths and 100% ethanol most of > them are falling off. Slides are silanised. > > I do not think it was an adhesion problem. What do you think about? > Embedding, cuttibg? > > Thanks a lot in advance > > Pablo > > > --- > El software de antivirus Avast ha analizado este correo electr?nico en busca de virus. > https://www.avast.com/antivirus > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Notice: This e-mail message, together with any attachments, contains > information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, > New Jersey, USA 07033), and/or its affiliates Direct contact information > for affiliates is available at > http://www.merck.com/contact/contacts.html) that may be confidential, > proprietary copyrighted and/or legally privileged. It is intended solely > for the use of the individual or entity named on this message. If you are > not the intended recipient, and have received this message in error, > please notify us immediately by reply e-mail and then delete it from > your system. --- El software de antivirus Avast ha analizado este correo electr?nico en busca de virus. https://www.avast.com/antivirus From Kristopher.Kalleberg at unilever.com Wed Mar 22 13:32:53 2017 From: Kristopher.Kalleberg at unilever.com (Kalleberg, Kristopher) Date: Wed, 22 Mar 2017 18:32:53 +0000 Subject: [Histonet] COL3A1 Message-ID: Hello, All, Curious if anyone knows of a great COL3A1 (Collagen III) antibody that works well in IHC for paraffin sections for human skin. I have tested a few and the ones that work/stain seem to stain most of the dermis of the human skin and I am concerned that they are staining for other collagens like Collagen I. If anyone can suggest one that they have used confidently it would be greatly appreciated. Thank you in advance. From philip_manfre at merck.com Wed Mar 22 14:30:09 2017 From: philip_manfre at merck.com (Manfre, Philip) Date: Wed, 22 Mar 2017 19:30:09 +0000 Subject: [Histonet] Sections falling off In-Reply-To: <4f9a26a3-fa0d-b5f9-c4d0-86a0bd30417b@usc.es> References: <3e496e32-7735-e770-5bdc-1fa8925879f6@usc.es> <4f9a26a3-fa0d-b5f9-c4d0-86a0bd30417b@usc.es> Message-ID: You can use 60 degrees if they are not for immunohistochemistry (IHC). If they are for IHC, you need to be more cautious with temperature. Some antigens are adversely affected or destroyed by excessive heat. Histochemistry should work fine heating the slides that much and that would probably aid adhesion. Phil. -----Original Message----- From: Pablo S?nchez Quinteiro via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, March 22, 2017 2:29 PM To: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Sections falling off Thanks for your hint Philip, They are stored at 37? overnight. I learnt the problem with the trapped water. I am very careful with it but they keep spoiling. They seem transparent. Could I try 60 minutes at 60? oven just before xylen? Pablo El 22/03/2017 a las 18:53, Manfre, Philip escribi?: > > -----Original Message----- > From: Pablo S?nchez Quinteiro via Histonet [mailto:histonet at lists.utsouthwestern.edu] > Sent: Wednesday, March 22, 2017 1:39 PM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Sections falling off > > Dear Histonetters, > > I am in trouble with my paraffin sections -samples fixed in Bouin's liquid. > > Pablo, > I think your sections are not completely dry, after placing them on slides. Silanized slides can trap water between the sections and the slide, almost appearing like a bulge. You may need to use the corner of a paper towel to "poke" the paraffin section and drain off the excess water. Make sure the sections look dry before starting the staining procedure. They should be transparent (translucent), not opaque (milky white). > That's my guess > > Phil. > > Philip Manfre, HT (ASCP) > Associate Principal Scientist > Merck Research Laboratories > WP81-406 > 770 Sumneytown Pike > West Point, PA 19486 > > 215-652-9750 > 215-993-0383 (fax) > philip_manfre at merck.com > > > > > Just after the first steps: Two xylol baths and 100% ethanol most of > them are falling off. Slides are silanised. > > I do not think it was an adhesion problem. What do you think about? > Embedding, cuttibg? > > Thanks a lot in advance > > Pablo > > > --- > El software de antivirus Avast ha analizado este correo electr?nico en busca de virus. > https://www.avast.com/antivirus > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Notice: This e-mail message, together with any attachments, contains > information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, > New Jersey, USA 07033), and/or its affiliates Direct contact information > for affiliates is available at > http://www.merck.com/contact/contacts.html) that may be confidential, > proprietary copyrighted and/or legally privileged. It is intended solely > for the use of the individual or entity named on this message. If you are > not the intended recipient, and have received this message in error, > please notify us immediately by reply e-mail and then delete it from > your system. --- El software de antivirus Avast ha analizado este correo electr?nico en busca de virus. https://www.avast.com/antivirus _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From lucie.s.guernsey at gmail.com Wed Mar 22 16:51:28 2017 From: lucie.s.guernsey at gmail.com (Lucie Guernsey) Date: Wed, 22 Mar 2017 14:51:28 -0700 Subject: [Histonet] Use of sodium borohydride in immunofluorescence In-Reply-To: References: Message-ID: Hi Bob, Yes, thanks for looking out! We're very careful with it (used only in the fume hood and we collect the waste for pickup) and right now I'm just updating protocols. So many of them are outdated and missing steps or helpful info. Thank you, also, for the best wishes. Lucie On Wed, Mar 22, 2017 at 7:12 AM, Bob Richmond via Histonet < histonet at lists.utsouthwestern.edu> wrote: > > > > Lucie Guernsey at UC San Diego asks about sodium borohydride: > > >>I'm currently going over IHC SOPs that were written by lab members that > have long ago moved on to other jobs. One inconsistency that I've come > across is the use of 0.5 % sodium borohydride in immunofluorescence.<< > > Does your protocol include the information that sodium borohydride is a > serious hazmat? Here's the Sigma-Aldrich MSDS: > http://www.sigmaaldrich.com/catalog/product/sigma/s9125?lang=en®ion=US > > >>There's a chance I have pregnancy brain....<< Best wishes to you and your > child! > > Bob Richmond > Samurai Pathologist > Maryville TN > > > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From pablo.sanchez at usc.es Wed Mar 22 19:06:12 2017 From: pablo.sanchez at usc.es (SANCHEZ QUINTEIRO PABLO) Date: Thu, 23 Mar 2017 00:06:12 +0000 Subject: [Histonet] Sections falling off In-Reply-To: <502052F0C384624FA5F2EF37D686AACC46CEBAAC@msgb09.nih.gov> References: <3e496e32-7735-e770-5bdc-1fa8925879f6@usc.es> <219fa61b472c4c30a8eedff28237dcd2@spiderman.MercerU.local> <99d89ba4-75ad-5187-c4ed-fff5a913bddc@usc.es>, <502052F0C384624FA5F2EF37D686AACC46CEBAAC@msgb09.nih.gov> Message-ID: Lots of thanks Ruth! What you tell us seems very sensible. But I am unsure about your protocol. After 24 h Bouin we transfer the brain to 70 alcohol and start embedding several days later. Must we avoid this storage in 70 ethanol? Instead must we wash the samples from Bouin after 24 h in water and start embedding from that point? Thanks for your input. Pablo ________________________________ De: Yaskovich, Ruth A (NIH/NIDCR) [E] Enviado: mi?rcoles, 22 de marzo de 2017 20:58:35 Para: SANCHEZ QUINTEIRO PABLO Asunto: RE: [Histonet] Sections falling off Pablo, Don't leave them in 70 rinse the bouin's out you can rinse cassettes in running water and then put on processor. Alcohol really dries out the CNS tissues and can cause that falling off the slide. I have been there. Good Luck Ruth N.I.H. -----Original Message----- From: Pablo S?nchez Quinteiro via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, March 22, 2017 2:22 PM To: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Sections falling off Thanks for your reply Shirley. After 24 h fixation in Bouin samples are passed into 70? ethanol. I have tried drying the sections at RT for short or long period (30?) and then 37? oven overnight. The tissue is mouse brain, sections thickness 8 microns. Pablo El 22/03/2017 a las 18:45, Shirley A. Powell escribi?: > Are you washing the bouins out of the tissue well before processing, that may be one source. Also how long are you drying your sections before staining? What type of tissue are you processing? > > -----Original Message----- > From: Pablo S?nchez Quinteiro via Histonet > [mailto:histonet at lists.utsouthwestern.edu] > Sent: Wednesday, March 22, 2017 1:39 PM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Sections falling off > > Dear Histonetters, > > I am in trouble with my paraffin sections -samples fixed in Bouin's liquid. > > Just after the first steps: Two xylol baths and 100% ethanol most of them are falling off. Slides are silanised. > > I do not think it was an adhesion problem. What do you think about? > Embedding, cuttibg? > > Thanks a lot in advance > > Pablo > > > --- > El software de antivirus Avast ha analizado este correo electr?nico en busca de virus. > https://www.avast.com/antivirus > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet --- El software de antivirus Avast ha analizado este correo electr?nico en busca de virus. https://www.avast.com/antivirus _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From G.Spoelstra at murdoch.edu.au Wed Mar 22 22:04:12 2017 From: G.Spoelstra at murdoch.edu.au (Gerard Spoelstra) Date: Thu, 23 Mar 2017 03:04:12 +0000 Subject: [Histonet] Sections falling off In-Reply-To: References: <3e496e32-7735-e770-5bdc-1fa8925879f6@usc.es> <219fa61b472c4c30a8eedff28237dcd2@spiderman.MercerU.local> <99d89ba4-75ad-5187-c4ed-fff5a913bddc@usc.es>, <502052F0C384624FA5F2EF37D686AACC46CEBAAC@msgb09.nih.gov>, Message-ID: Hi Pablo I have found with bone sections, which I pick up with silanised slided, that if I apply gelatin to the slides just prior to picking up the sections, the sections don't lift. The slides are dipped in the gelatin solution, then the sections are picked up off the surface of the water. I use roughly 0.02 of a gram of gelatin/ 100 ml water dissolved with microwave. The gelatin seems to alter the surface tension of the water as there is no pooling around the edges of the paraffin. The sections are then dried for a hour in a 60 degree oven, before staining. Gerard Spoelstra Veterinary Histology Murdoch University Western Australia ________________________________________ From: SANCHEZ QUINTEIRO PABLO via Histonet [histonet at lists.utsouthwestern.edu] Sent: Thursday, March 23, 2017 8:06 AM To: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Sections falling off Lots of thanks Ruth! What you tell us seems very sensible. But I am unsure about your protocol. After 24 h Bouin we transfer the brain to 70 alcohol and start embedding several days later. Must we avoid this storage in 70 ethanol? Instead must we wash the samples from Bouin after 24 h in water and start embedding from that point? Thanks for your input. Pablo ________________________________ De: Yaskovich, Ruth A (NIH/NIDCR) [E] Enviado: mi?rcoles, 22 de marzo de 2017 20:58:35 Para: SANCHEZ QUINTEIRO PABLO Asunto: RE: [Histonet] Sections falling off Pablo, Don't leave them in 70 rinse the bouin's out you can rinse cassettes in running water and then put on processor. Alcohol really dries out the CNS tissues and can cause that falling off the slide. I have been there. Good Luck Ruth N.I.H. -----Original Message----- From: Pablo S?nchez Quinteiro via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, March 22, 2017 2:22 PM To: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Sections falling off Thanks for your reply Shirley. After 24 h fixation in Bouin samples are passed into 70? ethanol. I have tried drying the sections at RT for short or long period (30?) and then 37? oven overnight. The tissue is mouse brain, sections thickness 8 microns. Pablo El 22/03/2017 a las 18:45, Shirley A. Powell escribi?: > Are you washing the bouins out of the tissue well before processing, that may be one source. Also how long are you drying your sections before staining? What type of tissue are you processing? > > -----Original Message----- > From: Pablo S?nchez Quinteiro via Histonet > [mailto:histonet at lists.utsouthwestern.edu] > Sent: Wednesday, March 22, 2017 1:39 PM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Sections falling off > > Dear Histonetters, > > I am in trouble with my paraffin sections -samples fixed in Bouin's liquid. > > Just after the first steps: Two xylol baths and 100% ethanol most of them are falling off. Slides are silanised. > > I do not think it was an adhesion problem. What do you think about? > Embedding, cuttibg? > > Thanks a lot in advance > > Pablo > > > --- > El software de antivirus Avast ha analizado este correo electr?nico en busca de virus. > https://www.avast.com/antivirus > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet --- El software de antivirus Avast ha analizado este correo electr?nico en busca de virus. https://www.avast.com/antivirus _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pablo.sanchez at usc.es Thu Mar 23 04:36:23 2017 From: pablo.sanchez at usc.es (=?UTF-8?Q?Pablo_S=c3=a1nchez_Quinteiro?=) Date: Thu, 23 Mar 2017 10:36:23 +0100 Subject: [Histonet] Sections falling off In-Reply-To: References: <3e496e32-7735-e770-5bdc-1fa8925879f6@usc.es> <4f9a26a3-fa0d-b5f9-c4d0-86a0bd30417b@usc.es> Message-ID: Yes I do... if they do not fall off in the hydration. Pablo El 22/03/2017 a las 20:04, Ingles Claire escribi?: > Pablo > Do you wash the slides in water before staining to remove the Bouin's (until the sections are no longer yellow)? I seem to remember that needs to be done. > Claire > > ________________________________________ > From: Pablo S?nchez Quinteiro via Histonet [histonet at lists.utsouthwestern.edu] > Sent: Wednesday, March 22, 2017 1:29 PM > To: histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] Sections falling off > > Thanks for your hint Philip, > > They are stored at 37? overnight. I learnt the problem with the trapped > water. I am very careful with it but they keep spoiling. > > They seem transparent. > > Could I try 60 minutes at 60? oven just before xylen? > > Pablo > > > El 22/03/2017 a las 18:53, Manfre, Philip escribi?: > >> -----Original Message----- >> From: Pablo S?nchez Quinteiro via Histonet [mailto:histonet at lists.utsouthwestern.edu] >> Sent: Wednesday, March 22, 2017 1:39 PM >> To: histonet at lists.utsouthwestern.edu >> Subject: [Histonet] Sections falling off >> >> Dear Histonetters, >> >> I am in trouble with my paraffin sections -samples fixed in Bouin's liquid. >> >> Pablo, >> I think your sections are not completely dry, after placing them on slides. Silanized slides can trap water between the sections and the slide, almost appearing like a bulge. You may need to use the corner of a paper towel to "poke" the paraffin section and drain off the excess water. Make sure the sections look dry before starting the staining procedure. They should be transparent (translucent), not opaque (milky white). >> That's my guess >> >> Phil. >> >> Philip Manfre, HT (ASCP) >> Associate Principal Scientist >> Merck Research Laboratories >> WP81-406 >> 770 Sumneytown Pike >> West Point, PA 19486 >> >> 215-652-9750 >> 215-993-0383 (fax) >> philip_manfre at merck.com >> >> >> >> >> Just after the first steps: Two xylol baths and 100% ethanol most of >> them are falling off. Slides are silanised. >> >> I do not think it was an adhesion problem. What do you think about? >> Embedding, cuttibg? >> >> Thanks a lot in advance >> >> Pablo >> >> >> --- >> El software de antivirus Avast ha analizado este correo electr?nico en busca de virus. >> https://www.avast.com/antivirus >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> Notice: This e-mail message, together with any attachments, contains >> information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, >> New Jersey, USA 07033), and/or its affiliates Direct contact information >> for affiliates is available at >> http://www.merck.com/contact/contacts.html) that may be confidential, >> proprietary copyrighted and/or legally privileged. It is intended solely >> for the use of the individual or entity named on this message. If you are >> not the intended recipient, and have received this message in error, >> please notify us immediately by reply e-mail and then delete it from >> your system. > > --- > El software de antivirus Avast ha analizado este correo electr?nico en busca de virus. > https://www.avast.com/antivirus > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet. > --- El software de antivirus Avast ha analizado este correo electr?nico en busca de virus. https://www.avast.com/antivirus From pablo.sanchez at usc.es Thu Mar 23 04:39:46 2017 From: pablo.sanchez at usc.es (=?UTF-8?Q?Pablo_S=c3=a1nchez_Quinteiro?=) Date: Thu, 23 Mar 2017 10:39:46 +0100 Subject: [Histonet] Sections falling off In-Reply-To: <72d0913dc5bd4e1cbe36327fabf10269@spiderman.MercerU.local> References: <3e496e32-7735-e770-5bdc-1fa8925879f6@usc.es> <219fa61b472c4c30a8eedff28237dcd2@spiderman.MercerU.local> <99d89ba4-75ad-5187-c4ed-fff5a913bddc@usc.es> <72d0913dc5bd4e1cbe36327fabf10269@spiderman.MercerU.local> Message-ID: <39135d10-b060-9b00-9ed2-282b4e42676d@usc.es> Thanks a lot Shirley, How long you wash in water the tissue before embedding? Where does the tissue come from? 70 alcohol? Pablo El 22/03/2017 a las 20:06, Shirley A. Powell escribi?: > I cut autopsy material for the medical examiners here and they are usually very bloody and thick sections. I face my blocks, soak them in an ice slush for 30 min to an hour or on a moist towel in the freezer overnight. I section them at 5 microns, a little thinner than yours. I use a product called Sta-On from Leica, it was a Surgipath product but Leica bought them out. I use a 10 ml to 990 ml water in my water bath. Charged slides or coated ones do not hold the bloody tissues on as well, so I use plain slides. After sectioning I stand them up for all the water to drain from between the section and the slide. Next I place on a hot plate/slide warmer at a temp just above the melting point of my paraffin to help the section relax and smooth out the micro folds that may be in the section. This takes s just a few minutes to melt the paraffin from around and in the section. I then place in the slide staining rack and place in an hot air slide dryer for at least an hour, but for me, I leave them overnight at 60 degrees. The next morning I stain my sections. > > Brain is a tissue that does not adhere as well as others to the slide so when staining be very gentle, agitate them in each solution but I would not agitate vigorously. > > The reason I asked about washing the bouin's out, is that it could change the charge on your slides and affect the stain. I was always taught to wash the tissue before beginning the dehydration in the processing so that residual picric acid would not affect my staining as well. > > Hope this makes sense and helps. > > Shirley > > -----Original Message----- > From: Pablo S?nchez Quinteiro via Histonet [mailto:histonet at lists.utsouthwestern.edu] > Sent: Wednesday, March 22, 2017 2:22 PM > To: histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] Sections falling off > > Thanks for your reply Shirley. > > After 24 h fixation in Bouin samples are passed into 70? ethanol. > > I have tried drying the sections at RT for short or long period (30?) and then 37? oven overnight. > > The tissue is mouse brain, sections thickness 8 microns. > > Pablo > > > > El 22/03/2017 a las 18:45, Shirley A. Powell escribi?: >> Are you washing the bouins out of the tissue well before processing, that may be one source. Also how long are you drying your sections before staining? What type of tissue are you processing? >> >> -----Original Message----- >> From: Pablo S?nchez Quinteiro via Histonet >> [mailto:histonet at lists.utsouthwestern.edu] >> Sent: Wednesday, March 22, 2017 1:39 PM >> To: histonet at lists.utsouthwestern.edu >> Subject: [Histonet] Sections falling off >> >> Dear Histonetters, >> >> I am in trouble with my paraffin sections -samples fixed in Bouin's liquid. >> >> Just after the first steps: Two xylol baths and 100% ethanol most of them are falling off. Slides are silanised. >> >> I do not think it was an adhesion problem. What do you think about? >> Embedding, cuttibg? >> >> Thanks a lot in advance >> >> Pablo >> >> >> --- >> El software de antivirus Avast ha analizado este correo electr?nico en busca de virus. >> https://www.avast.com/antivirus >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > --- > El software de antivirus Avast ha analizado este correo electr?nico en busca de virus. > https://www.avast.com/antivirus > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet --- El software de antivirus Avast ha analizado este correo electr?nico en busca de virus. https://www.avast.com/antivirus From pablo.sanchez at usc.es Thu Mar 23 04:40:39 2017 From: pablo.sanchez at usc.es (=?UTF-8?Q?Pablo_S=c3=a1nchez_Quinteiro?=) Date: Thu, 23 Mar 2017 10:40:39 +0100 Subject: [Histonet] Sections falling off In-Reply-To: <32285988.1701489.1490211215233@mail.yahoo.com> References: <3e496e32-7735-e770-5bdc-1fa8925879f6@usc.es> <32285988.1701489.1490211215233@mail.yahoo.com> Message-ID: Thanks a lot! El 22/03/2017 a las 20:33, LitePath escribi?: > Hi Pablo > This article just came out, I actually have not read yet, just > downloaded yesterday...thought I would send to your way as it might be > helpful... > > Salute > Luis > Luis Chiriboga PhD > Director IHC > Experimental Pathology > NYULMC > > > ------------------------------------------------------------------------ > *From:* Pablo S?nchez Quinteiro via Histonet > > *To:* histonet at lists.utsouthwestern.edu > *Sent:* Wednesday, March 22, 2017 1:50 PM > *Subject:* [Histonet] Sections falling off > > Dear Histonetters, > > I am in trouble with my paraffin sections -samples fixed in Bouin's > liquid. > > Just after the first steps: Two xylol baths and 100% ethanol most of > them are falling off. Slides are silanised. > > I do not think it was an adhesion problem. What do you think about? > Embedding, cuttibg? > > Thanks a lot in advance > > Pablo > > > --- > El software de antivirus Avast ha analizado este correo electr?nico en > busca de virus. > https://www.avast.com/antivirus > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > --- El software de antivirus Avast ha analizado este correo electr?nico en busca de virus. https://www.avast.com/antivirus From j.benavides at eae.csic.es Thu Mar 23 06:56:46 2017 From: j.benavides at eae.csic.es (Julio Benavides) Date: Thu, 23 Mar 2017 12:56:46 +0100 Subject: [Histonet] Sections falling off In-Reply-To: References: <3e496e32-7735-e770-5bdc-1fa8925879f6@usc.es> <32285988.1701489.1490211215233@mail.yahoo.com> Message-ID: <0d08d5b0-e6ee-e1ed-1fee-7d1bbae3af94@eae.csic.es> Just to add another advice, Pablo, yesterday I told you that we were drying the slides overnight at 37?... not true!! (that happens for me speaking without checking with the really knowledgeable ones, the technicians!! We used to have that same problem and then began to dry at 60? for one hour straight after cutting, then, after that hour in the oven straight to staining. We don?t wash under tap water, as we have problems with variable water pressure, so instead, 3x5 min wash. Hope this helps Cheers, Julio El 23/03/2017 a las 10:40, Pablo S?nchez Quinteiro via Histonet escribi?: > Thanks a lot! > > > El 22/03/2017 a las 20:33, LitePath escribi?: >> Hi Pablo >> This article just came out, I actually have not read yet, just >> downloaded yesterday...thought I would send to your way as it might >> be helpful... >> >> Salute >> Luis >> Luis Chiriboga PhD >> Director IHC >> Experimental Pathology >> NYULMC >> >> >> ------------------------------------------------------------------------ >> *From:* Pablo S?nchez Quinteiro via Histonet >> >> *To:* histonet at lists.utsouthwestern.edu >> *Sent:* Wednesday, March 22, 2017 1:50 PM >> *Subject:* [Histonet] Sections falling off >> >> Dear Histonetters, >> >> I am in trouble with my paraffin sections -samples fixed in Bouin's >> liquid. >> >> Just after the first steps: Two xylol baths and 100% ethanol most of >> them are falling off. Slides are silanised. >> >> I do not think it was an adhesion problem. What do you think about? >> Embedding, cuttibg? >> >> Thanks a lot in advance >> >> Pablo >> >> >> --- >> El software de antivirus Avast ha analizado este correo electr?nico >> en busca de virus. >> https://www.avast.com/antivirus >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> > > > > --- > El software de antivirus Avast ha analizado este correo electr?nico en > busca de virus. > https://www.avast.com/antivirus > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From marielachertoff at gmail.com Thu Mar 23 08:28:24 2017 From: marielachertoff at gmail.com (Mariela Chertoff) Date: Thu, 23 Mar 2017 10:28:24 -0300 Subject: [Histonet] low signal for long post fixation Message-ID: Dear all, I have mouse brain tissue perfused with PFA 4% and post fixed in formol 10% for periods between 2 month and 1 year. I cut them on vibratome (30um) and I made inmunohistochemistry and beta galactosidase to check senescence. My problem is that I have less signal on positive controls if postfixation on formol was longer. There is a way to solve this problem? Thanks in advance for your reply Mariela Chertoff, PhD Laboratorio de Neuroepigenetica - QB75 Departamento de Qu?mica Biol?gica Facultad de Ciencias Exactas y Naturales - UBA Ciudad Universitaria Pabell?n II Piso 4 Ciudad Aut?noma de Buenos Aires C1428EGA - Argentina Tel: 54 11 4576-3300/09 - Int. 221 email:marielachertoff at gmail.com marielachertoff at qb.fcen.uba.ar From rjbuesa at yahoo.com Thu Mar 23 09:25:32 2017 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Thu, 23 Mar 2017 14:25:32 +0000 (UTC) Subject: [Histonet] low signal for long post fixation In-Reply-To: References: Message-ID: <22212280.1389259.1490279132571@mail.yahoo.com> Perhaps your only solution is to increase HIARRen? On Thursday, March 23, 2017 9:39 AM, Mariela Chertoff via Histonet wrote: Dear all, I have mouse brain tissue perfused with PFA 4% and post fixed in formol 10% for periods between 2 month and 1 year. I cut them on vibratome (30um) and I made inmunohistochemistry and beta galactosidase to check senescence. My problem is that I have less signal on positive controls if postfixation on formol was longer. There is a way to solve this problem? Thanks in advance for your reply Mariela Chertoff, PhD Laboratorio de Neuroepigenetica - QB75 Departamento de Qu?mica Biol?gica Facultad de Ciencias Exactas y Naturales - UBA Ciudad Universitaria Pabell?n II Piso 4 Ciudad Aut?noma de Buenos Aires C1428EGA - Argentina Tel: 54 11 4576-3300/09 - Int. 221 email:marielachertoff at gmail.com marielachertoff at qb.fcen.uba.ar _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lmarie08 at uga.edu Thu Mar 23 10:05:56 2017 From: lmarie08 at uga.edu (Lauren Sweeney) Date: Thu, 23 Mar 2017 15:05:56 +0000 Subject: [Histonet] solvent recyclers Message-ID: Hi everyone, We are looking into getting a new solvent recycler to recycle our xylene and alcohol, any recommendations? Thanks! From koellingr at comcast.net Thu Mar 23 10:21:55 2017 From: koellingr at comcast.net (koellingr at comcast.net) Date: Thu, 23 Mar 2017 15:21:55 +0000 (UTC) Subject: [Histonet] low signal for long post fixation In-Reply-To: References: Message-ID: <504309046.11369607.1490282515392.JavaMail.zimbra@comcast.net> Mariela, (1) If you are looking at enzyme activity of a LacZ transgene to localize with X-gal staining, I found the situation really a problem with extended fixation times of vibratome sections (like you with controls or test article). Have never been able to "retrieve" that kind of staining. Maybe someone out there knows the trick. Especially bad in low-level integration of transgene. (2) I have though gone after the enzyme with just traditional IHC to B-galactosidase (there are good antibodies available. And as Rene said, increase your antigen retrieval which will (might, should) bring back the antigenic sites for IHC. But for the enzyme activity, I've never had much luck at that. Ray Koelling Faculty lecturer, WWAMI, UW School of Medicine, Spokane, WA ----- Original Message ----- From: "Mariela Chertoff via Histonet" To: histonet at lists.utsouthwestern.edu Sent: Thursday, March 23, 2017 6:28:24 AM Subject: [Histonet] low signal for long post fixation Dear all, I have mouse brain tissue perfused with PFA 4% and post fixed in formol 10% for periods between 2 month and 1 year. I cut them on vibratome (30um) and I made inmunohistochemistry and beta galactosidase to check senescence. My problem is that I have less signal on positive controls if postfixation on formol was longer. There is a way to solve this problem? Thanks in advance for your reply Mariela Chertoff, PhD Laboratorio de Neuroepigenetica - QB75 Departamento de Qu?mica Biol?gica Facultad de Ciencias Exactas y Naturales - UBA Ciudad Universitaria Pabell?n II Piso 4 Ciudad Aut?noma de Buenos Aires C1428EGA - Argentina Tel: 54 11 4576-3300/09 - Int. 221 email:marielachertoff at gmail.com marielachertoff at qb.fcen.uba.ar _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang at gmx.at Thu Mar 23 11:44:44 2017 From: gu.lang at gmx.at (Gudrun Lang) Date: Thu, 23 Mar 2017 17:44:44 +0100 Subject: [Histonet] WG: question about retina IHC In-Reply-To: <0068fa2cfe7e41c8a9096919297c7227@KUKXS02.kepleruniklinikum.at> References: <0068fa2cfe7e41c8a9096919297c7227@KUKXS02.kepleruniklinikum.at> Message-ID: <002401d2a3f4$c7d99980$578ccc80$@gmx.at> Because I have no experience with this special specimens, I give the question below to the kind histonet and its professionals. Gudrun Lang Von: Amirkavei Mooud [mailto:mooud.amirkavei at aalto.fi] Gesendet: Donnerstag, 23. M?rz 2017 13:02 An: Lang Gudrun Betreff: question about retina IHC Wichtigkeit: Hoch Dear Gurdun, I am a Ph.D student from Kai Kaarniranta?s grou, Finland. I am working with mouse retina and need to do retina staining to check desired changes in POS. Now I would like to ask your help. Would it be possible for you to help me with the protocol of preparation of sample for retina sectioning and all procedure of its IHC. I don?t know if I can use snap freezing and cryosectioning or using PFA and normal microtome? I appreciate very much your kind help J. Bets regards, Mooud From rjbuesa at yahoo.com Thu Mar 23 12:02:34 2017 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Thu, 23 Mar 2017 17:02:34 +0000 (UTC) Subject: [Histonet] solvent recyclers In-Reply-To: References: Message-ID: <153656339.2123702.1490288554354@mail.yahoo.com> B/R recyclersRen? On Thursday, March 23, 2017 11:15 AM, Lauren Sweeney via Histonet wrote: Hi everyone, We are looking into getting a new solvent recycler to recycle our xylene and alcohol, any recommendations? Thanks! _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek at digestivespecialists.com Thu Mar 23 13:15:46 2017 From: lblazek at digestivespecialists.com (Blazek, Linda) Date: Thu, 23 Mar 2017 14:15:46 -0400 Subject: [Histonet] solvent recyclers In-Reply-To: References: Message-ID: <5A2BD13465E061429D6455C8D6B40E39198C961FED@IBMB7Exchange.digestivespecialists.com> I have had the recycler from CBG for years and it's been a great workhorse! Linda Blazek HT (ASCP) Pathology Lab Manager GI Pathology of Dayton Phone: (937) 396-2623 Email: lblazek at digestivespecialists.com -----Original Message----- From: Lauren Sweeney via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, March 23, 2017 11:06 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] solvent recyclers Hi everyone, We are looking into getting a new solvent recycler to recycle our xylene and alcohol, any recommendations? Thanks! _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond at gmail.com Fri Mar 24 13:00:50 2017 From: rsrichmond at gmail.com (Bob Richmond) Date: Fri, 24 Mar 2017 14:00:50 -0400 Subject: [Histonet] solvent recyclers Message-ID: A few years ago the B/R spinning-band stills were definitely the still of choice for recovering histologic solvents, and the older generation of CBG machine were inadequate. I think though that the later generation of CBG stills predominates today. I don't know how they work. Could somebody give us an update on this subject? Bob Richmond Samurai Pathologist Maryville TN From garethdavisyuma at gmail.com Fri Mar 24 13:34:08 2017 From: garethdavisyuma at gmail.com (Gareth Davis) Date: Fri, 24 Mar 2017 11:34:08 -0700 Subject: [Histonet] solvent recyclers In-Reply-To: References: Message-ID: I have a CBG recycler and it has never given me any problems in the two years I have been here. The last two labs I worked in (in the past 15 years) both had CBG recyclers and loved them. Gareth Davis On Fri, Mar 24, 2017 at 11:00 AM, Bob Richmond via Histonet < histonet at lists.utsouthwestern.edu> wrote: > A few years ago the B/R spinning-band stills were definitely the still of > choice for recovering histologic solvents, and the older generation of CBG > machine were inadequate. I think though that the later generation of CBG > stills predominates today. I don't know how they work. Could somebody give > us an update on this subject? > > Bob Richmond > Samurai Pathologist > Maryville TN > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Ms. Gareth B. Davis, B.S., HT, QIHC (ASCP)cm Yuma Gastroenterology Yuma, AZ 85364 928-248-5259 From liz at premierlab.com Fri Mar 24 14:00:51 2017 From: liz at premierlab.com (Elizabeth Chlipala) Date: Fri, 24 Mar 2017 13:00:51 -0600 Subject: [Histonet] solvent recyclers - long response Message-ID: <14E2C6176416974295479C64A11CB9AE0302C0353E4D@SBS2K8.premierlab.local> Bob I believe that both the B/R an CBG recyclers are fractional distillation units, and to be honest I'm not even certain why I choose the CBG over B/R instrument when I purchased. Prior to purchasing the CBG I was using the Suncycle Technologies system, these less expensive systems are more like filters and are not fractional distillation units, For alcohol percentage you get out what you put in. We have been using the CBG recycler for many years now, we recycle our 95 and 100 alcohol (I think it was around 2008 or so when we purchased it - we have a 5 liter system) - adding lower percentage alcohols to the mix just decreases the alcohol percentage of the output. We also recycle our xylene and propar. We do have defined SOP's that govern everything and we test the xylene for water content and record lot numbers for the xylene generated. The alcohol is checked with a hydrometer, adjusted for temp prior to making up lower percentage alcohols, and again we document with in house generated lot number for the lower grade alcohols we make. We use the recycled xylene and alcohol in both the stainer and tissue processor, we even use recycled xylene in the coverslipper. The only place we do not use recycled xylene is in the cleaning xylene station on the tissue processor we use new xylene for that. Our instrument is serviced every other year, we are a low volume lab. Recycling has saved us money in reagents and in disposal, since we started recycling our waste classification changed from small quantity generator to conditionally exempt small quantity generator, so that saves us in additional fees for a state waste certificate and other regulatory documentation, training, etc. that is required once you become a small quantity generator. In my opinion and I would say our clients opinions also, recycling has not affected the quality of our product, we process very small samples such as cartilage pellets up to large 2 x 3 slide research samples like lion vocal cords or portions of canine mandibles. We have done extensive testing on the precision and accuracy of our IHC and H&E staining via image analysis and the quality of our processing, routine, special and IHC is consistent and has not been affected at all since we began recycling. I firmly believe that you need to manage the reagents accordingly and make sure you follow the instructions and limitations of the instrument, for example you MUST not recycle any alcohol that has been used in deparaffinization. The documentation that we require does take some time but in our environment as GLP compliant lab it is required. There has been some extensive discussion on the value of recycling recently on the histonet and I would say that there are individuals that will not agree with my opinion, but based upon our experience it does works for us and I feel that it is of value personally. On another note - Congrats on your retirement!! Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: Bob Richmond via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, March 24, 2017 12:01 PM To: Histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] solvent recyclers A few years ago the B/R spinning-band stills were definitely the still of choice for recovering histologic solvents, and the older generation of CBG machine were inadequate. I think though that the later generation of CBG stills predominates today. I don't know how they work. Could somebody give us an update on this subject? Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the Symantec Email Security.cloud service. For more information please visit http://www.symanteccloud.com ______________________________________________________________________ From ian.bernard at comcast.net Fri Mar 24 23:04:08 2017 From: ian.bernard at comcast.net (ian bernard) Date: Fri, 24 Mar 2017 22:04:08 -0600 Subject: [Histonet] Reference for this Recipe and Use Message-ID: <008901d2a51c$dca3df00$95eb9d00$@comcast.net> 500 ML OF 100% ALCOHOL 170 ML OF DISTILLED WATER 80 ML OF 40% FORMALIN 50 ML OF GLACIAL ACETIC ACID V/R IB From tony.henwood at health.nsw.gov.au Sun Mar 26 18:22:31 2017 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Sun, 26 Mar 2017 23:22:31 +0000 Subject: [Histonet] Reference for this Recipe and Use In-Reply-To: <008901d2a51c$dca3df00$95eb9d00$@comcast.net> References: <008901d2a51c$dca3df00$95eb9d00$@comcast.net> Message-ID: <0237449DE79DBC45B686AB82CDCD16FF014EAD20@SVDCMBX-MEX008.nswhealth.net> This is sometimes known as Newel's solution for revealing lymph nodes in fatty specimens: Newell KJ, Sawka BW, Rudrick BF, Driman DK. (2001) "GEWF solution" Arch Pathol Lab Med 125:642 645. Absolute ethanol 1000ml Water 340ml Concentrated formalin (37%) 160ml Glacial acetic acid 100ml Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: ian bernard via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Saturday, 25 March 2017 3:04 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Reference for this Recipe and Use 500 ML OF 100% ALCOHOL 170 ML OF DISTILLED WATER 80 ML OF 40% FORMALIN 50 ML OF GLACIAL ACETIC ACID V/R IB _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From j.rowaihi at alborglaboratories.com Mon Mar 27 04:39:56 2017 From: j.rowaihi at alborglaboratories.com (Jamal Rowaihi) Date: Mon, 27 Mar 2017 12:39:56 +0300 Subject: [Histonet] (no subject) Message-ID: Hi colleagues?We are going for Digital Pathology?What is your recommendation?What's the best device and company (Aperio, Philips, 3DHISTECH....) Regards Jamal RowaihiAnatomic Pathology SupervisorAl Borg Medical Laboratories?Sent from my cell phone From redrose297 at gmail.com Mon Mar 27 04:55:31 2017 From: redrose297 at gmail.com (warda hassan) Date: Mon, 27 Mar 2017 09:55:31 +0000 Subject: [Histonet] Dako Auto stainer for PAP Message-ID: Hi to all We are planning to move on to Dako Auto stainer for PAP; any one can share their experience from staining quality to difficulty in handling the autostainer Good day Thank you Rose Histopathology Royal- Oman From brannon at alliedsearchpartners.com Mon Mar 27 13:38:28 2017 From: brannon at alliedsearchpartners.com (Brannon Owens) Date: Mon, 27 Mar 2017 18:38:28 +0000 Subject: [Histonet] Histology Support Specialist (IHC) job opening in Baltimore/DC Message-ID: I have a full time/permanent opportunity for an experienced IHC Histology professional in the Baltimore/DC area. This individual would be traveling about 75% of the time with company car, gas card, and travel expenses reimbursed while traveling. The remainder of the time would be spent working from home. A full job description is listed on our website. Interested candidates Nationwide are encouraged to apply as there is also a $5,000 relocation assistance offered for the right candidate. To view a complete list of Allied Search Partners current openings go to: http://www.alliedsearchpartners.com/careers/ Brannon Owens Recruitment Manager Allied Search Partners LinkedIn: http://www.linkedin.com/pub/brannon-owens/28/528/823 http://www.alliedsearchpartners.com T: 888.388.7571 ext. 106 Direct Line: 407.413.9421 F: 888.388.7572 From canderton at biocare.net Mon Mar 27 13:45:51 2017 From: canderton at biocare.net (Caryn Anderton) Date: Mon, 27 Mar 2017 18:45:51 +0000 Subject: [Histonet] I have a job Message-ID: Hello- I wanted to know if it was possible to post a position that I have available? See Below---- Immunohistochemistry Product Manager Communications Expert- Part Time Summary: The position will focus on working with three internal groups within Biocare Medical: Marketing, Engineering and R&D. We're looking for a driven, resourceful technical writing and communication expert to manage the process of writing our customer-facing product documentation. You'll manage the end-to-end process of drafting, writing, and releasing documentation, tutorials, developer guides for: internal and client trainings, sales collateral and product manuals. Engineering & R&D: Collaborate with the Engineering team to launch a new automated IHC slide staining platform. Engage with the teams to understand the instrument and how it works and be able to translate technical concepts, and make them easy to understand for our end users. Create and maintain reference and user guides, including hardware and software documentation Marketing: Work with marketing to supply scientific knowledge and technical/medical writing for product catalogs, marketing brochures and website content. Work with training department to develop sales training programs. Requirements: ? Broad knowledge base in Histology , Immunohistochemistry and related instrumentation ? Excellent writing, editing, and proofreading skills with meticulous attention to detail ? Ability to present information and generate content appropriate market segments ? Self-motivated, independent, able to prioritize and manage multiple projects ? Demonstrate capacity to work collaboratively within cross-functional teams ? Proven and consistent track record of meeting project deadlines with quality results ? Excellent organizational and time management skills ? Ability to receive creative direction and feedback ? Please include a cover letter and curriculum-writing sample with your application. Biocare Medical is a leading supplier of automated immunohistochemistry (IHC) instrumentation, antibodies and the full range of reagents for IHC. Biocare's portfolio includes novel and established primary antibodies and FISH probes for many tissue types. EEO/AA Employer/Veterans/Disabled/Race/Ethnicity/Gender/Age To all recruitment agencies: Biocare does not accept agency resumes. Biocare will not pay for any fees related to unsolicited resumes. Job Location-Pacheco, California, United States Department-Marketing Position Type-Part Time Caryn M. Anderton Senior Director of Marketing Biocare Medical, LLC. 60 Berry Dr. Pacheco, CA 94553 (925) 768-1270 www.biocare.net From canderton at biocare.net Mon Mar 27 13:54:03 2017 From: canderton at biocare.net (Caryn Anderton) Date: Mon, 27 Mar 2017 18:54:03 +0000 Subject: [Histonet] JOB LISTING Message-ID: The job is located in Pacheco California please send resume to canderton at biocare.net no phone calls Immunohistochemistry Writer Communications Expert- Part Time Summary: The position will focus on working with three internal groups within Biocare Medical: Marketing, Engineering and R&D. We're looking for a driven, resourceful technical writing and communication expert to manage the process of writing our customer-facing product documentation. You'll manage the end-to-end process of drafting, writing, and releasing documentation, tutorials, developer guides for: internal and client trainings, sales collateral and product manuals. Engineering & R&D: Collaborate with the Engineering team to launch a new automated IHC slide staining platform. Engage with the teams to understand the instrument and how it works and be able to translate technical concepts, and make them easy to understand for our end users. Create and maintain reference and user guides, including hardware and software documentation Marketing: Work with marketing to supply scientific knowledge and technical/medical writing for product catalogs, marketing brochures and website content. Work with training department to develop sales training programs. Requirements: ? Broad knowledge base in Histology , Immunohistochemistry and related instrumentation ? Excellent writing, editing, and proofreading skills with meticulous attention to detail ? Ability to present information and generate content appropriate market segments ? Self-motivated, independent, able to prioritize and manage multiple projects ? Demonstrate capacity to work collaboratively within cross-functional teams ? Proven and consistent track record of meeting project deadlines with quality results ? Excellent organizational and time management skills ? Ability to receive creative direction and feedback ? Please include a cover letter and curriculum-writing sample with your application. Biocare Medical is a leading supplier of automated immunohistochemistry (IHC) instrumentation, antibodies and the full range of reagents for IHC. Biocare's portfolio includes novel and established primary antibodies and FISH probes for many tissue types. EEO/AA Employer/Veterans/Disabled/Race/Ethnicity/Gender/Age To all recruitment agencies: Biocare does not accept agency resumes. Biocare will not pay for any fees related to unsolicited resumes. Job Location-Pacheco, California, United States Department-Marketing Position Type-Part Time Caryn M. Anderton Senior Director of Marketing Biocare Medical, LLC. 60 Berry Dr. Pacheco, CA 94553 canderton at biocare.net www.biocare.net From CIngles at uwhealth.org Mon Mar 27 15:01:36 2017 From: CIngles at uwhealth.org (Ingles Claire) Date: Mon, 27 Mar 2017 20:01:36 +0000 Subject: [Histonet] Reference for this Recipe and Use In-Reply-To: <0237449DE79DBC45B686AB82CDCD16FF014EAD20@SVDCMBX-MEX008.nswhealth.net> References: <008901d2a51c$dca3df00$95eb9d00$@comcast.net>, <0237449DE79DBC45B686AB82CDCD16FF014EAD20@SVDCMBX-MEX008.nswhealth.net> Message-ID: Sadly, it is much safer than the old Chloroform clearing method. Claire ________________________________________ From: Tony Henwood (SCHN) via Histonet [histonet at lists.utsouthwestern.edu] Sent: Sunday, March 26, 2017 6:22 PM To: ian bernard Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Reference for this Recipe and Use This is sometimes known as Newel's solution for revealing lymph nodes in fatty specimens: Newell KJ, Sawka BW, Rudrick BF, Driman DK. (2001) "GEWF solution" Arch Pathol Lab Med 125:642 645. Absolute ethanol 1000ml Water 340ml Concentrated formalin (37%) 160ml Glacial acetic acid 100ml Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: ian bernard via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Saturday, 25 March 2017 3:04 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Reference for this Recipe and Use 500 ML OF 100% ALCOHOL 170 ML OF DISTILLED WATER 80 ML OF 40% FORMALIN 50 ML OF GLACIAL ACETIC ACID V/R IB _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nguy0515 at gmail.com Mon Mar 27 15:52:10 2017 From: nguy0515 at gmail.com (Trini Nguyen) Date: Mon, 27 Mar 2017 20:52:10 +0000 Subject: [Histonet] MITF for Mohs frozen sections Message-ID: Hello all, Mohs surgeon would like to start doing MITF (microphthalmia transcription factor) for melanoma with a chromogen yielding red results manually. I have never done them manually and do not know where to begin. We have a bond max but it keeps breaking down and you can't stain manually since the AEC and DAB are mixed by the stainer itself. Please help with any information and or procedure/protocols you have. From akemiat3377 at gmail.com Mon Mar 27 16:50:41 2017 From: akemiat3377 at gmail.com (Eileen Akemi Allison) Date: Mon, 27 Mar 2017 14:50:41 -0700 Subject: [Histonet] I have a job In-Reply-To: References: Message-ID: I thought we weren?t allowed to post jobs on histonet! I tried and it was removed! Akemi Allison-Tacha > On Mar 27, 2017, at 11:45 AM, Caryn Anderton via Histonet wrote: > > Hello- > > I wanted to know if it was possible to post a position that I have available? See Below---- > > Immunohistochemistry Product Manager Communications Expert- Part Time > > Summary: > The position will focus on working with three internal groups within Biocare Medical: Marketing, Engineering and R&D. We're looking for a driven, resourceful technical writing and communication expert to manage the process of writing our customer-facing product documentation. You'll manage the end-to-end process of drafting, writing, and releasing documentation, tutorials, developer guides for: internal and client trainings, sales collateral and product manuals. > Engineering & R&D: > Collaborate with the Engineering team to launch a new automated IHC slide staining platform. Engage with the teams to understand the instrument and how it works and be able to translate technical concepts, and make them easy to understand for our end users. Create and maintain reference and user guides, including hardware and software documentation > Marketing: > Work with marketing to supply scientific knowledge and technical/medical writing for product catalogs, marketing brochures and website content. Work with training department to develop sales training programs. > Requirements: > > ? Broad knowledge base in Histology , Immunohistochemistry and related instrumentation > > ? Excellent writing, editing, and proofreading skills with meticulous attention to detail > > ? Ability to present information and generate content appropriate market segments > > ? Self-motivated, independent, able to prioritize and manage multiple projects > > ? Demonstrate capacity to work collaboratively within cross-functional teams > > ? Proven and consistent track record of meeting project deadlines with quality results > > ? Excellent organizational and time management skills > > ? Ability to receive creative direction and feedback > > ? Please include a cover letter and curriculum-writing sample with your application. > Biocare Medical is a leading supplier of automated immunohistochemistry (IHC) instrumentation, antibodies and the full range of reagents for IHC. Biocare's portfolio includes novel and established primary antibodies and FISH probes for many tissue types. > EEO/AA Employer/Veterans/Disabled/Race/Ethnicity/Gender/Age > > To all recruitment agencies: Biocare does not accept agency resumes. Biocare will not pay for any fees related to unsolicited resumes. > > Job Location-Pacheco, California, United States > Department-Marketing > Position Type-Part Time > > > > > > > Caryn M. Anderton > Senior Director of Marketing > Biocare Medical, LLC. > 60 Berry Dr. > Pacheco, CA 94553 > (925) 768-1270 > www.biocare.net > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akemiat3377 at gmail.com Mon Mar 27 16:52:12 2017 From: akemiat3377 at gmail.com (Eileen Akemi Allison) Date: Mon, 27 Mar 2017 14:52:12 -0700 Subject: [Histonet] I have a job In-Reply-To: References: Message-ID: <506245E6-A602-464F-BFF6-992D39F6DE51@gmail.com> I thought we weren?t allowed to post jobs on histonet! I tried and it was removed! Akemi Allison-Tacha > On Mar 27, 2017, at 11:45 AM, Caryn Anderton via Histonet wrote: > > Hello- > > I wanted to know if it was possible to post a position that I have available? See Below---- > > Immunohistochemistry Product Manager Communications Expert- Part Time > > Summary: > The position will focus on working with three internal groups within Biocare Medical: Marketing, Engineering and R&D. We're looking for a driven, resourceful technical writing and communication expert to manage the process of writing our customer-facing product documentation. You'll manage the end-to-end process of drafting, writing, and releasing documentation, tutorials, developer guides for: internal and client trainings, sales collateral and product manuals. > Engineering & R&D: > Collaborate with the Engineering team to launch a new automated IHC slide staining platform. Engage with the teams to understand the instrument and how it works and be able to translate technical concepts, and make them easy to understand for our end users. Create and maintain reference and user guides, including hardware and software documentation > Marketing: > Work with marketing to supply scientific knowledge and technical/medical writing for product catalogs, marketing brochures and website content. Work with training department to develop sales training programs. > Requirements: > > ? Broad knowledge base in Histology , Immunohistochemistry and related instrumentation > > ? Excellent writing, editing, and proofreading skills with meticulous attention to detail > > ? Ability to present information and generate content appropriate market segments > > ? Self-motivated, independent, able to prioritize and manage multiple projects > > ? Demonstrate capacity to work collaboratively within cross-functional teams > > ? Proven and consistent track record of meeting project deadlines with quality results > > ? Excellent organizational and time management skills > > ? Ability to receive creative direction and feedback > > ? Please include a cover letter and curriculum-writing sample with your application. > Biocare Medical is a leading supplier of automated immunohistochemistry (IHC) instrumentation, antibodies and the full range of reagents for IHC. Biocare's portfolio includes novel and established primary antibodies and FISH probes for many tissue types. > EEO/AA Employer/Veterans/Disabled/Race/Ethnicity/Gender/Age > > To all recruitment agencies: Biocare does not accept agency resumes. Biocare will not pay for any fees related to unsolicited resumes. > > Job Location-Pacheco, California, United States > Department-Marketing > Position Type-Part Time > > > > > > > Caryn M. Anderton > Senior Director of Marketing > Biocare Medical, LLC. > 60 Berry Dr. > Pacheco, CA 94553 > (925) 768-1270 > www.biocare.net > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From NRich at bmhsc.org Tue Mar 28 10:09:12 2017 From: NRich at bmhsc.org (Nina J. Rich) Date: Tue, 28 Mar 2017 15:09:12 +0000 Subject: [Histonet] Primera Cassette Printer Message-ID: Anyone have a Primera Cassette Printer? We have slide printer and cassette printer and have had to replace the cassette printer 4 times already. Would like to know likes and dislikes and problems other labs might be having with their printers. My phone number is 843-522-5159. Any information would greatly be appreciated. Thanks. Jean From jaylundgren at gmail.com Tue Mar 28 15:02:23 2017 From: jaylundgren at gmail.com (Jay Lundgren) Date: Tue, 28 Mar 2017 16:02:23 -0400 Subject: [Histonet] I have a job In-Reply-To: <506245E6-A602-464F-BFF6-992D39F6DE51@gmail.com> References: <506245E6-A602-464F-BFF6-992D39F6DE51@gmail.com> Message-ID: As far as I know, looking for a job is frowned upon, posting jobs is OK, once in awhile. This isn't Indeed.com On Mon, Mar 27, 2017 at 5:52 PM, Eileen Akemi Allison via Histonet < histonet at lists.utsouthwestern.edu> wrote: > I thought we weren?t allowed to post jobs on histonet! I tried and it was > removed! > > Akemi Allison-Tacha > > > On Mar 27, 2017, at 11:45 AM, Caryn Anderton via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > > > > Hello- > > > > I wanted to know if it was possible to post a position that I have > available? See Below---- > > > > Immunohistochemistry Product Manager Communications Expert- Part Time > > > > Summary: > > The position will focus on working with three internal groups within > Biocare Medical: Marketing, Engineering and R&D. We're looking for a > driven, resourceful technical writing and communication expert to manage > the process of writing our customer-facing product documentation. You'll > manage the end-to-end process of drafting, writing, and releasing > documentation, tutorials, developer guides for: internal and client > trainings, sales collateral and product manuals. > > Engineering & R&D: > > Collaborate with the Engineering team to launch a new automated IHC > slide staining platform. Engage with the teams to understand the instrument > and how it works and be able to translate technical concepts, and make them > easy to understand for our end users. Create and maintain reference and > user guides, including hardware and software documentation > > Marketing: > > Work with marketing to supply scientific knowledge and technical/medical > writing for product catalogs, marketing brochures and website content. Work > with training department to develop sales training programs. > > Requirements: > > > > ? Broad knowledge base in Histology , Immunohistochemistry and related > instrumentation > > > > ? Excellent writing, editing, and proofreading skills with meticulous > attention to detail > > > > ? Ability to present information and generate content appropriate > market segments > > > > ? Self-motivated, independent, able to prioritize and manage multiple > projects > > > > ? Demonstrate capacity to work collaboratively within cross-functional > teams > > > > ? Proven and consistent track record of meeting project deadlines with > quality results > > > > ? Excellent organizational and time management skills > > > > ? Ability to receive creative direction and feedback > > > > ? Please include a cover letter and curriculum-writing sample with your > application. > > Biocare Medical is a leading supplier of automated immunohistochemistry > (IHC) instrumentation, antibodies and the full range of reagents for IHC. > Biocare's portfolio includes novel and established primary antibodies and > FISH probes for many tissue types. > > EEO/AA Employer/Veterans/Disabled/Race/Ethnicity/Gender/Age > > > > To all recruitment agencies: Biocare does not accept agency resumes. > Biocare will not pay for any fees related to unsolicited resumes. > > > > Job Location-Pacheco, California, United States > > Department-Marketing > > Position Type-Part Time > > > > > > > > > > > > > > Caryn M. Anderton > > Senior Director of Marketing > > Biocare Medical, LLC. > > 60 Berry Dr. > > Pacheco, CA 94553 > > (925) 768-1270 > > www.biocare.net > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From POWELL_SA at mercer.edu Tue Mar 28 15:20:14 2017 From: POWELL_SA at mercer.edu (Shirley A. Powell) Date: Tue, 28 Mar 2017 20:20:14 +0000 Subject: [Histonet] I have a job question Message-ID: <013c7c3e36034b9d89fa5ac4c1dc8bfb@spiderman.MercerU.local> Below is what is found when subscribing to histonet at http://lists.utsouthwestern.edu/mailman/listinfo/histonet. The two doctors who run histonet are highlighted below in yellow. They would be the ones who make the rules and those questions should be directed to them. There are no hard and fast rules here to define who can post and what can be posted. Sometimes subscribers think they know the rules of the listserve, but need to ask the ones who are in charge first what they are. Maybe they would be kind enough to share them with us. Histonet -- For the exchange of information pertaining to histotechnology and related fields About Histonet English (USA) WHO SHOULD SUBSCRIBE? Anyone interested in research or clinical applications of histology, immunohistochemistry, in-situ hybridization pathology, and electron microscopy may find Histonet informative and useful. Currently, there are more than 3200 subscribers from all over the world. Subscribers include hospital employees from major urban centers and obscure remote locales, university researchers, botanists and the employees of commercial laboratories, government agencies, veterinary facilities and a wide variety of commercial industrial ventures. WHO RUNS HISTONET? The list is run by Anita Sengupta M.D. and Linda R. Margraf, M.D. using hardware and software owned by the University of Texas Southwestern Medical School in Dallas, Texas. If you have any questions or problems with Histonet please contact Linda Margraf at lindamargraf at gmail.com To see the collection of prior postings to the list, visit the Histonet Archives. Using Histonet To post a message to all the list members, send email to histonet at lists.utsouthwestern.edu. You can subscribe to the list, or change your existing subscription, in the sections below. Subscribing to Histonet Subscribe to Histonet by filling out the following form. You will be sent email requesting confirmation, to prevent others from gratuitously subscribing you. This is a hidden list, which means that the list of members is available only to the list administrator. Your email address: Your name (optional): You may enter a privacy password below. This provides only mild security, but should prevent others from messing with your subscription. Do not use a valuable password as it will occasionally be emailed back to you in cleartext. If you choose not to enter a password, one will be automatically generated for you, and it will be sent to you once you've confirmed your subscription. You can always request a mail-back of your password when you edit your personal options. Pick a password: Reenter password to confirm: Which language do you prefer to display your messages? English (USA) Would you like to receive list mail batched in a daily digest? No Yes Histonet Subscribers (The subscribers list is only available to the list administrator.) Enter your admin address and password to visit the subscribers list: Admin address: Password: To unsubscribe from Histonet, get a password reminder, or change your subscription options enter your subscription email address: If you leave the field blank, you will be prompted for your email address ________________________________ From ctorrence at kmcpa.com Tue Mar 28 17:15:55 2017 From: ctorrence at kmcpa.com (Carol Torrence) Date: Tue, 28 Mar 2017 22:15:55 +0000 Subject: [Histonet] Grossing histotech opening - Topeka, Kansas Message-ID: Good day all! We have an opening in our histology lab! We are a smaller lab with a fun and fast pace. Besides routine histology, we offer our dermatopathologist a broad menu of IHC testing. The lab operates Monday through Friday with a team of 5. Weekends are free to enjoy nearby lakes, parks and arts. We are 28 miles from Lawrence, 50 miles from Manhattan and 65 miles from greater Kansas City. Candidate must meet CLIA requirements for grossing (high complexity). Follow this link to check us out! http://www.kmcpa.com/ Follow this link for a complete job description https://www.indeed.com/viewjob?jk=e07c4cac6b004db4&tk=1bb4jhvlqak8leja&from=company This notification is being placed by the employer and not an employment agency. Have a great day! Carol M. Torrence, HT(ASCP) Director of Lab Services From Mariam.Vanetsyan at cshs.org Tue Mar 28 18:01:32 2017 From: Mariam.Vanetsyan at cshs.org (Vanetsyan, Mariam) Date: Tue, 28 Mar 2017 23:01:32 +0000 Subject: [Histonet] Intensity of Mayer's Hematoxylin. Message-ID: Good Afternoon Everyone, I have been using Modified Mayer's Hematoxylin, preformulated as a counterstain for IHC: 5 min in HEM Followed by 10 sec rinse running DI Water 20 sec in Ammonium Hydroxide (30%) diluted 5 drops in 250 mL DI Water My question: How can I increase the intensity of BLUE color? Thank you, Mariam Vanetsyan Cedars-Sinai Medical Center| 8723 Alden Dr. Los Angeles, CA 90048 IMPORTANT WARNING: This message is intended for the use of the person or entity to which it is addressed and may contain information that is privileged and confidential, the disclosure of which is governed by applicable law. If the reader of this message is not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this information is strictly prohibited. Thank you for your cooperation. From mward at wakehealth.edu Wed Mar 29 11:47:43 2017 From: mward at wakehealth.edu (Martha Ward-Pathology) Date: Wed, 29 Mar 2017 16:47:43 +0000 Subject: [Histonet] Looking for a C4d ffpe control block Message-ID: Hello everyone, I am trying to find someone who can share a C4d ffpe control block with us. Ours is almost gone and I don't have access to any suitable tissues in house. If anyone has a block they are willing to share please contact me. I can try to help out with any controls that you may be in need of as well. Thanks in advance for everyone's help. Martha Ward, MT (ASCP) QIHC Manager, Molecular Diagnostics Lab Wake Forest Baptist Health Winston-Salem, NC 27157 mward at wakehealth.edu From wangweixi at sjhmc.org Wed Mar 29 12:09:25 2017 From: wangweixi at sjhmc.org (Wang, Weixi) Date: Wed, 29 Mar 2017 17:09:25 +0000 Subject: [Histonet] formalin pad for grossing Message-ID: <1CC147AA7F0DC04B92CF569DB81256A9DEC156E4@INFEXDB02.sjhmc.sjhealthsys.org> Dear all Histonetters, It has been a while since I joined the mailing list and I learned a lot here! Now I have a question and would like to get feedbacks regarding the use of formalin in the grossing room. I was instructed to use the formalin-neutralized pads during the grossing. Any other pads or paper, absorbent or not, cannot go to the bio-hazardous waste. But the neutralizer pads are so costly, especially for grossing part, when we need to change very frequently to void the cross contamination between specimen. I would like to know what is the pad/mat/paper you use for grossing, and how do you dispose these formalin contaminated materials. Any feedback is deeply appreciated! Weixi Weixi Wang, Ph.D., HTL(ASCP) Manager | Histology Laboratory St. Joseph's Healthcare System 703 Main Street, Paterson, NJ 07503 Phone: 973.754.3541 | Fax: 973.754.3292 wangweixi at sjhmc.org | www.StJosephsHealth.org *** Important Notice About St. Josephs emails *** St. Josephs Regional Medical Center is using Zix to encrypt any e-mails containing Protected Health Information (PHI). If you receive an encrypted email from a person at St. Josephs the body of the message will indicate that you have a "New Zixcorp secure email from St. Josephs Regional Medical Center." You will need to click on the link in that email to retrieve the message. For more information please visit http://www.uapguide.com/st-josephs-hospital-and-regional-medical-center/introduction. For help in retrieving a secure email that you??ve received from St Josephs please go to http://www.uapguide.com/st-josephs-hospital-and-regional-medical-center/receiving-encrypted-email. CONFIDENTIAL COMMUNICATION THIS TRANSMISSION IS INTENDED ONLY FOR THE INDIVIDUAL OR ENTITY TO WHICH IT IS ADDRESSED AND CONTAINS INFORMATION THAT IS CONFIDENTIAL. IF YOU HAVE RECEIVED THIS COMMUNICATION IN ERROR, PLEASE DELETE THE EMAIL AND CONTACT THE SENDER IMMEDIATELY. THIS INFORMATION MAY HAVE BEEN DISCLOSED TO YOU FROM CONFIDENTIAL RECORDS AND MAY BE PROTECTED BY FEDERAL AND STATE LAW. THIS INFORMATION MAY INCLUDE CONFIDENTIAL MENTAL HEALTH, SUBSTANCE ABUSE, ALCOHOL ABUSE AND/OR HIV-RELATED INFORMATION. FEDERAL AND STATE LAW PROHIBITS YOU FROM MAKING ANY FURTHER DISCLOSURE OF THIS INFORMATION WITHOUT THE SPECIFIC WRITTEN CONSENT OF THE PERSON TO WHOM IT PERTAINS, OR AS OTHERWISE PERMITTED BY LAW. ANY UNAUTHORIZED FURTHER DISCLOSURE IN VIOLATION OF THE LAW MAY RESULT IN A FINE OR JAIL SENTENCE OR BOTH. A GENERAL AUTHORIZATION FOR THE RELEASE OF THIS INFORMATION MAY NOT BE SUFFICIENT AUTHORIZATION FOR FURTHER DISCLOSURE. From tejohnson at genoptix.com Wed Mar 29 12:37:10 2017 From: tejohnson at genoptix.com (Teri Johnson) Date: Wed, 29 Mar 2017 17:37:10 +0000 Subject: [Histonet] Intensity of Mayer's Hematoxylin Message-ID: Hi Mariam, Mayer's is a progressive hematoxylin. Therefore you should increase your time in the stain to get a deeper color. You might try 10 or 20 minutes and see what works best for you. You can do your bluing in tap water or even buffer solution rather than ammonium hydroxide if you prefer. It's not as fast but potentially more gentle on the tissue. If you want a more intense blue (be careful you don't obscure light nuclear positivity), you could use a different hematoxylin stain solution. Best wishes, Teri ________________________________ CONFIDENTIALITY NOTICE: The sender of this email is not an employee or agent of, or a contractor or consultant to, Genoptix. This email has been sent through the Genoptix email system as a transitional service provided by Genoptix, Inc. ("Genoptix") to Navigate BioPharma Services, Inc. The sender of this email is not authorized to represent or to speak on behalf of any member of Genoptix, nor to enter into any undertaking, arrangement or agreement on behalf of any member of Genoptix. Any such undertaking, arrangement or agreement shall not be binding on Genoptix. Genoptix accepts no liability for the content of this email, or for the consequences of any acts or omissions taken or not taken on the basis of the information contained in this email or in any email sent from this email address. All reasonable precautions have been taken to ensure no viruses or other malware are present in this e-mail and Genoptix cannot accept responsibility for any loss or damage arising from the use of this e-mail or attachments and you are responsible for ensuring that your own virus screening procedures are up to date and fit for purpose. From mneglia at trajanscimed.com Wed Mar 29 12:38:46 2017 From: mneglia at trajanscimed.com (Marc Neglia) Date: Thu, 30 Mar 2017 04:38:46 +1100 Subject: [Histonet] Pathology slide survey - AMAZON GIFT CARD OFFER Message-ID: <61E7E1B920537D4BABCCDF8D8B6E70D1062B2A2FD9E3@mifune1> Hello Histonetters, I am conducting a study to learn about your experiences in the laboratory with microscope slides. The information gathered will help assist with product development decisions that are aimed at improving patient outcomes and optimizing your pathology workflow. To participate in this short, 5 minute survey, please click on the link below: https://www.surveymonkey.com/r/BKNBBWV As a token of appreciation, we will randomly select four (4) respondents to receive a $50 Amazon gift card after the survey has been completed. I would like to thank you in advance for your participation, and I look forward to viewing your responses. Best regards, Marc Marc Neglia Trajan Scientific and Medical mneglia at trajanscimed.com www.trajanscimed.com From mpence at grhs.net Wed Mar 29 12:53:53 2017 From: mpence at grhs.net (Mike Pence) Date: Wed, 29 Mar 2017 17:53:53 +0000 Subject: [Histonet] Pathology slide survey - AMAZON GIFT CARD OFFER In-Reply-To: <61E7E1B920537D4BABCCDF8D8B6E70D1062B2A2FD9E3@mifune1> References: <61E7E1B920537D4BABCCDF8D8B6E70D1062B2A2FD9E3@mifune1> Message-ID: This looks to me like a way to get contact information to make sales calls later! -----Original Message----- From: Marc Neglia via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, March 29, 2017 12:40 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Pathology slide survey - AMAZON GIFT CARD OFFER Hello Histonetters, I am conducting a study to learn about your experiences in the laboratory with microscope slides. The information gathered will help assist with product development decisions that are aimed at improving patient outcomes and optimizing your pathology workflow. To participate in this short, 5 minute survey, please click on the link below: https://www.surveymonkey.com/r/BKNBBWV As a token of appreciation, we will randomly select four (4) respondents to receive a $50 Amazon gift card after the survey has been completed. I would like to thank you in advance for your participation, and I look forward to viewing your responses. Best regards, Marc Marc Neglia Trajan Scientific and Medical mneglia at trajanscimed.com www.trajanscimed.com _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From beller at uw.edu Wed Mar 29 18:42:46 2017 From: beller at uw.edu (Allison Beller) Date: Wed, 29 Mar 2017 16:42:46 -0700 Subject: [Histonet] Anatomic Pathology Technologist Role at UW Medicine Message-ID: The UW Neuropathology Core has an outstanding opportunity for a full time *ANATOMIC PATHOLOGY TECHNOLOGIST* This is an excellent opportunity for an experienced technologist to perform complex procedures for services such as histology, brain autopsy, and/or specialized quantitative fluid and tissue based immunoassays. Under the general supervision of the Principle Investigator and the Lab Manager the licensed anatomic pathology technologist will perform histotechnology procedures such as processing, embedding, cutting, mounting, and staining of tissue specimens for evaluation and diagnosis by Pathologists. In addition, the anatomic pathology technologist will perform complex procedures in the specialty service area of brain autopsy including complex fresh and fixed brain dissections for histology and other qualitative and quantitative analyses. In addition to histology, histochemistry, and immunohistochemistry procedures, duties will include performing autopsy procedures specific to the central nervous system such as brain removal, sectioning, dissection, slicing, and sampling, and neuropathologic data recording and/or in depth involvement in quantitative immunoassays in the lab including Luminex, flow cytometry, and/or Histelide. The successful applicant will be required to perform special stains via automated stainer and or manual staining; use care and maintain histology instruments; perform quality control and quality assurance; perform research and assist in development of new procedures; monitor reagent use, perform preventative maintenance on instruments according to prescribed protocols. The successful applicant will be expected to organize and maintain updated databases on tissue blocks and other materials and to navigate the laboratory and pathology computer networks, participate in lab and other meetings, provide regular progress updates, review work lists and prioritize work in conjunction with the PI and lab manager, and interact with the research team and collaborators. REQUIREMENTS: A Bachelor's Degree with coursework in chemistry, biology and other biological sciences including microscopy AND certification , or certified within two years of date of hire, at the Technologist level by a nationally recognized certifying agency such as the American Society of Clinical Pathologists, the American Society of Clinical Laboratory Scientists or the Microscopy Society of America AND one year of clinical anatomic laboratory experience OR equivalent education/experience. DESIRED: HT or HTL certified by the American Society of Clinical Pathologist (ASCP) Experience or familiarity with Power-Path To learn more and apply see UW job requisition #143582 https://uwhires.admin.washington.edu/eng/candidates/default.cfm?szCategory=jobprofile&szOrderID=143582&szCandidateID=0&szSearchWords=&szReturnToSearch=1 Allison Beller Research Manager University of Washington UW Medicine | Neuropathology email: beller at uw.edu From tbraud at holyredeemer.com Thu Mar 30 12:42:55 2017 From: tbraud at holyredeemer.com (Terri Braud) Date: Thu, 30 Mar 2017 17:42:55 +0000 Subject: [Histonet] disposable grossing pad Message-ID: <48E053DDF6CE074DB6A7414BA05403F812D064@HRHEX03-HOS.holyredeemer.local> I know many, including staff here, that prefer to use "chux". They are very cheap, come in various sizes and adsorption levels, latex-free, with a highly absorbent core and soft, quick-drying coverstock so you don't get lint in your specimens. Use your favorite search engine to look them up. Almost all major Med suppliers carry them. Between specimens, we just toss the old one into red-bag waste. I hope this helps. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal 1. formalin pad for grossing (Wang, Weixi) ---------------------------------------------------------------------- Message: 1 Date: Wed, 29 Mar 2017 17:09:25 +0000 From: "Wang, Weixi" Subject: [Histonet] formalin pad for grossing Dear all Histonetters, It has been a while since I joined the mailing list and I learned a lot here! Now I have a question and would like to get feedbacks regarding the use of formalin in the grossing room. I was instructed to use the formalin-neutralized pads during the grossing. Any other pads or paper, absorbent or not, cannot go to the bio-hazardous waste. But the neutralizer pads are so costly, especially for grossing part, when we need to change very frequently to void the cross contamination between specimen. I would like to know what is the pad/mat/paper you use for grossing, and how do you dispose these formalin contaminated materials. Any feedback is deeply appreciated! Weixi Weixi Wang, Ph.D., HTL(ASCP) Manager | Histology Laboratory St. Joseph's Healthcare System 703 Main Street, Paterson, NJ 07503 Phone: 973.754.3541 | Fax: 973.754.3292 wangweixi at sjhmc.org | www.StJosephsHealth.org *** From jriggleman at globusmedical.com Thu Mar 30 16:20:38 2017 From: jriggleman at globusmedical.com (Jessica Riggleman) Date: Thu, 30 Mar 2017 21:20:38 +0000 Subject: [Histonet] Microscope Camera Message-ID: Hi Everyone, I am looking for a camera for a microscope (specifically for an Olympus BX41) to look at cellular detail. If you have one that is not in use please let me know. Thank You, Jessica _____________________________________________________________________ Jessica Riggleman | Research Associate Globus Medical, Inc. Valley Forge Business Center 2560 General Armistead Avenue | Audubon, PA 19403 Ph: (610) 930-1800 ext. 2583 | Fax: Confidentiality Note: This email is confidential and intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing, or copying of this email is strictly prohibited. If you have received this email in error please contact the sender. Any views or opinions presented are solely those of the author and do not necessarily represent those of Globus Medical, Inc. Although this email and any attachments are believed to be free of any virus or other defects which might affect any computer or IT system into which they are received, no responsibility is accepted by Globus Medical, Inc. for any loss or damage arising in any way from the receipt or use thereof. From thigginsht at msn.com Fri Mar 31 07:50:37 2017 From: thigginsht at msn.com (T H) Date: Fri, 31 Mar 2017 12:50:37 +0000 Subject: [Histonet] Tissue Fixation In-Reply-To: References: Message-ID: Good Morning, I have a pathologist that is not happy with the fixation on some of our LEEP specimens. She swears its histology doing something to the specimen to cause the tissue to look unfixed on only "part" of the LEEP specimens (all the same client specimens). She claims we must be diluting our formalin to cause this issue or "something". We mentioned maybe it was on the clients end not placing them in 10% formalin right away, she wouldn't hear of it. Let me give you some back ground on how our process works. Our clients send us all our specimens to us via Overnight FedEx or UPS in 10% formalin they will then they sit in 10% formalin in-house until the processors are started around 3pm and sits an additional 5 hours in 10% formalin on the processor before the processor actually starts. That being said the fixation process has had a pretty good start before we ever even touch it. My question is, and I thought I had read this in the past is, when a specimen is left out prior to fixation and lying on a absorbent surface such as a paper towel, won't the area of the tissue touching the absorbent surface begin the disintegration processes faster in that exact area then the rest of the specimen? Or if you have any other suggestion on what might be happening to only "certain" specimens would be great as well. Thanks for your help! Tim From rjbuesa at yahoo.com Fri Mar 31 08:32:35 2017 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Fri, 31 Mar 2017 13:32:35 +0000 (UTC) Subject: [Histonet] Tissue Fixation In-Reply-To: References: Message-ID: <1730848134.8065268.1490967155655@mail.yahoo.com> What you describe as a possible scenario is absolutely possible.If your PT does not "want to hear" about it, suggest she gets a "hearing aid" or to study something about histotechnology or even better yet, pay attention to what a professional on the subject (you) has to say about it. You would never dare to question her diagnosis, why would she question yours on this subject?Ren? On Friday, March 31, 2017 9:11 AM, T H via Histonet wrote: Good Morning, I have a pathologist that is not happy with the fixation on some of our LEEP specimens.? She swears its histology doing something to the specimen to cause the tissue to look unfixed on only "part" of the LEEP specimens (all the same client specimens).? She claims we must be diluting our formalin to cause this issue or "something".? We mentioned maybe it was on the clients end not placing them in 10% formalin right away, she wouldn't hear of it. Let me give you some back ground on how our process works.? Our clients send us all our specimens to us via Overnight FedEx or UPS in 10% formalin they will then they sit in 10% formalin in-house until the processors are started around 3pm and sits an additional 5 hours in 10% formalin on the processor before the processor actually starts.? That being said the fixation process has had a pretty good start before we ever even touch it. My question is, and I thought I had read this in the past is, when a specimen is left out prior to fixation and lying on a absorbent surface such as a paper towel, won't the area of the tissue touching the absorbent surface begin the disintegration processes faster in that exact area then the rest of the specimen?? Or if you have any other suggestion on what might be happening to only "certain" specimens would be great as well. Thanks for your help! Tim _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From paula at excaliburpathology.com Fri Mar 31 08:57:30 2017 From: paula at excaliburpathology.com (Paula Keene Pierce) Date: Fri, 31 Mar 2017 13:57:30 +0000 (UTC) Subject: [Histonet] Tissue Fixation In-Reply-To: <1730848134.8065268.1490967155655@mail.yahoo.com> References: <1730848134.8065268.1490967155655@mail.yahoo.com> Message-ID: <372734167.8016960.1490968650877@mail.yahoo.com> Bravo Rene!?Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953FAX 405-759-7513www.excaliburpathology.com From: Rene J Buesa via Histonet To: T H ; "histonet at lists.utsouthwestern.edu" Sent: Friday, March 31, 2017 8:44 AM Subject: Re: [Histonet] Tissue Fixation What you describe as a possible scenario is absolutely possible.If your PT does not "want to hear" about it, suggest she gets a "hearing aid" or to study something about histotechnology or even better yet, pay attention to what a professional on the subject (you) has to say about it. You would never dare to question her diagnosis, why would she question yours on this subject?Ren? ? ? On Friday, March 31, 2017 9:11 AM, T H via Histonet wrote: Good Morning, I have a pathologist that is not happy with the fixation on some of our LEEP specimens.? She swears its histology doing something to the specimen to cause the tissue to look unfixed on only "part" of the LEEP specimens (all the same client specimens).? She claims we must be diluting our formalin to cause this issue or "something".? We mentioned maybe it was on the clients end not placing them in 10% formalin right away, she wouldn't hear of it. Let me give you some back ground on how our process works.? Our clients send us all our specimens to us via Overnight FedEx or UPS in 10% formalin they will then they sit in 10% formalin in-house until the processors are started around 3pm and sits an additional 5 hours in 10% formalin on the processor before the processor actually starts.? That being said the fixation process has had a pretty good start before we ever even touch it. My question is, and I thought I had read this in the past is, when a specimen is left out prior to fixation and lying on a absorbent surface such as a paper towel, won't the area of the tissue touching the absorbent surface begin the disintegration processes faster in that exact area then the rest of the specimen?? Or if you have any other suggestion on what might be happening to only "certain" specimens would be great as well. Thanks for your help! Tim _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ? _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken at ucsf.edu Fri Mar 31 10:06:53 2017 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Fri, 31 Mar 2017 15:06:53 +0000 Subject: [Histonet] Tissue Fixation In-Reply-To: <1730848134.8065268.1490967155655@mail.yahoo.com> References: <1730848134.8065268.1490967155655@mail.yahoo.com> Message-ID: <761E2B5697F795489C8710BCC72141FF8E5C6461@ex07.net.ucsf.edu> Tim, I have to agree with Rene that the formalin or time in formalin is obviously not the problem - it has plenty of time in formalin (and who would dilute it anyway?). Handling before formalin must always be determined when problems arise. If the sample sits on a paper towel, gauze etc it does not really degrade faster, rather the tissue may dry out and so fixes by dehydration rather than by formalin. It may be the formalin cannot get into the tissue in those dried out portions of the tissue. However that is just speculation. Since these all seem to be from one particular client, the client is the place to start. The only way to determine the problem is to follow the specimen from start to finish. Can you or someone you trust physically observe the way samples are handled from the time they are taken to the time put in formalin? One issue I always run up against is people saying they do one thing but actually doing another. And they may realize during questioning that they are not doing it right but don't want to admit it. It wastes a lot of time. I've had physicians tell me to ignore the part of the process they are responsible for because they do it right. I just tell them that to be complete we need to follow the process from start to finish, nothing personal, just business. Leaving any part out may lead to not resolving the problem. Probably 99% of the process is just fine, but that 1% is damaging the sample and needs to be illuminated. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Rene J Buesa via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, March 31, 2017 6:33 AM To: T H; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Tissue Fixation What you describe as a possible scenario is absolutely possible.If your PT does not "want to hear" about it, suggest she gets a "hearing aid" or to study something about histotechnology or even better yet, pay attention to what a professional on the subject (you) has to say about it. You would never dare to question her diagnosis, why would she question yours on this subject?Ren? On Friday, March 31, 2017 9:11 AM, T H via Histonet wrote: Good Morning, I have a pathologist that is not happy with the fixation on some of our LEEP specimens.? She swears its histology doing something to the specimen to cause the tissue to look unfixed on only "part" of the LEEP specimens (all the same client specimens).? She claims we must be diluting our formalin to cause this issue or "something".? We mentioned maybe it was on the clients end not placing them in 10% formalin right away, she wouldn't hear of it. Let me give you some back ground on how our process works.? Our clients send us all our specimens to us via Overnight FedEx or UPS in 10% formalin they will then they sit in 10% formalin in-house until the processors are started around 3pm and sits an additional 5 hours in 10% formalin on the processor before the processor actually starts.? That being said the fixation process has had a pretty good start before we ever even touch it. My question is, and I thought I had read this in the past is, when a specimen is left out prior to fixation and lying on a absorbent surface such as a paper towel, won't the area of the tissue touching the absorbent surface begin the disintegration processes faster in that exact area then the rest of the specimen?? Or if you have any other suggestion on what might be happening to only "certain" specimens would be great as well. Thanks for your help! Tim _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joyce.weems at emoryhealthcare.org Fri Mar 31 10:26:23 2017 From: joyce.weems at emoryhealthcare.org (Weems, Joyce K.) Date: Fri, 31 Mar 2017 15:26:23 +0000 Subject: [Histonet] Tissue Fixation In-Reply-To: <761E2B5697F795489C8710BCC72141FF8E5C6461@ex07.net.ucsf.edu> References: <1730848134.8065268.1490967155655@mail.yahoo.com> <761E2B5697F795489C8710BCC72141FF8E5C6461@ex07.net.ucsf.edu> Message-ID: Aren't LEEPS done with some sort of electric method that will damage the tissue before it even reaches formalin. I'm not positive but Google it - I believe that might be the problem. j Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax 770-380-8099 Cell joyce.weems at emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph?s Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: Morken, Timothy via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, March 31, 2017 11:07 AM To: Rene J Buesa ; T H Cc: Histonet Subject: Re: [Histonet] Tissue Fixation Tim, I have to agree with Rene that the formalin or time in formalin is obviously not the problem - it has plenty of time in formalin (and who would dilute it anyway?). Handling before formalin must always be determined when problems arise. If the sample sits on a paper towel, gauze etc it does not really degrade faster, rather the tissue may dry out and so fixes by dehydration rather than by formalin. It may be the formalin cannot get into the tissue in those dried out portions of the tissue. However that is just speculation. Since these all seem to be from one particular client, the client is the place to start. The only way to determine the problem is to follow the specimen from start to finish. Can you or someone you trust physically observe the way samples are handled from the time they are taken to the time put in formalin? One issue I always run up against is people saying they do one thing but actually doing another. And they may realize during questioning that they are not doing it right but don't want to admit it. It wastes a lot of time. I've had physicians tell me to ignore the part of the process they are responsible for because they do it right. I just tell them that to be complete we need to follow the process from start to finish, nothing personal, just business. Leaving any part out may lead to not resolving the problem. Probably 99% of the process is just fine, but that 1% is damaging the sample and needs to be illuminated. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Rene J Buesa via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, March 31, 2017 6:33 AM To: T H; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Tissue Fixation What you describe as a possible scenario is absolutely possible.If your PT does not "want to hear" about it, suggest she gets a "hearing aid" or to study something about histotechnology or even better yet, pay attention to what a professional on the subject (you) has to say about it. You would never dare to question her diagnosis, why would she question yours on this subject?Ren? On Friday, March 31, 2017 9:11 AM, T H via Histonet wrote: Good Morning, I have a pathologist that is not happy with the fixation on some of our LEEP specimens. She swears its histology doing something to the specimen to cause the tissue to look unfixed on only "part" of the LEEP specimens (all the same client specimens). She claims we must be diluting our formalin to cause this issue or "something". We mentioned maybe it was on the clients end not placing them in 10% formalin right away, she wouldn't hear of it. Let me give you some back ground on how our process works. Our clients send us all our specimens to us via Overnight FedEx or UPS in 10% formalin they will then they sit in 10% formalin in-house until the processors are started around 3pm and sits an additional 5 hours in 10% formalin on the processor before the processor actually starts. That being said the fixation process has had a pretty good start before we ever even touch it. My question is, and I thought I had read this in the past is, when a specimen is left out prior to fixation and lying on a absorbent surface such as a paper towel, won't the area of the tissue touching the absorbent surface begin the disintegration processes faster in that exact area then the rest of the specimen? Or if you have any other suggestion on what might be happening to only "certain" specimens would be great as well. Thanks for your help! Tim _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From j.benavides at eae.csic.es Fri Mar 31 10:39:14 2017 From: j.benavides at eae.csic.es (Julio Benavides) Date: Fri, 31 Mar 2017 17:39:14 +0200 Subject: [Histonet] Update on Trimming soft brain In-Reply-To: <20170306231819.Horde.yLnzqmg1byVn79kW7me1SA1@webmail.csic.es> References: <20170306231819.Horde.yLnzqmg1byVn79kW7me1SA1@webmail.csic.es> Message-ID: <3eba3ac9-eced-57ee-c497-2ea55e1c3f73@eae.csic.es> Hi there, just to update you on the issue. Thanks to the kind and useful suggestions from histonetes, I found the solution to my problem. Fixation for two days in 10% formalin in absolute ethanol worked perfectly well. Brains were well fixed, we could trimmed them, and the fixation in alcoholic formalin did not interfere with the IHCs, at least with the one we are using (CD3, bAPP and GFAP). Again, thank you so much for your help! cheers Julio El 06/03/2017 a las 23:18, Julio Benavides via Histonet escribi?: > Hi there, > > I?m trying to trim ovine foetal brain (90 days of gestation). The > brain is > too soft and there is no way to get a proper slice. It has been in > buffered > 10% formalin for three weeks but still too soft. When you take the brain > out of the pot and put into the trimming table, it just kind of spill > over > the place. We have tried to embed the brain in 7.5% gelatin, which > helps to > keep the shape of the brain before trimming, but as soon you cut the > slice, > the centre of the tissue just melt away. I was wondering if there is any > post fixative solution we can try. May be Bouin?s? > > Any suggestion would be greatly appreciated! > > Cheers > > Julio > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jford at cytomx.com Fri Mar 31 10:55:57 2017 From: jford at cytomx.com (Judi Ford) Date: Fri, 31 Mar 2017 15:55:57 +0000 Subject: [Histonet] Tissue banking Message-ID: Hi everyone, Happy Friday to you all...... I am hoping to gather some information on how people bank frozen tissue for work in research. I am trying to start a bank of frozen human samples stored in a -80 freezer for our scientists use. Information that I'm looking for is as follows: 1. Accessioning software/database - what software do you use to keep track of samples and their useage. What information is useful for you in keeping track of the samples. 2. Storing samples in the freezer - how do you store them; wrapped foil, in cryoboxes, in ziplock baggies, etc. 3. Do you have a system of checking out the samples? If so, how do you keep track and control of the samples? 4. Do you have one person who is in charge of the tissue bank? If so what are their responsibilities? Thanks for your help with this. If you want to send me information offline here is my email address: jford at cytomx.com. Thank you and I hope you have a really great weekend. Judi Ford SRA CytomX Therapeutics STATEMENT OF CONFIDENTIALITY: The information contained in this electronic message and any attachments to this message are intended for the exclusive use of the addressee(s) and may contain confidential or privileged information. If you are not an intended recipient, or a person responsible for delivering the e-mail to an intended recipient, please be advised that you have received this message in error and that any use, dissemination, forwarding, printing, or copying is strictly prohibited. Please notify the sender at CytomX Therapeutics, Inc., immediately and destroy all copies of this message and any attachments. CytomX Therapeutics, Inc. From LRaff at uropartners.com Fri Mar 31 11:25:47 2017 From: LRaff at uropartners.com (Lester Raff MD) Date: Fri, 31 Mar 2017 16:25:47 +0000 Subject: [Histonet] Disposal of slides/blocks Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF11313D43@COLOEXCH01.uropartners.local> Our lab is once again evaluating disposal of slides/blocks from >10 years ago. Maintaining or donating the material do not appear to be feasible options. Aside from PHI issues, how are labs disposing of this type of material. Do you place the slides in sharps containers? Do you red bag the blocks? Thanks. Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From gu.lang at gmx.at Fri Mar 31 12:10:55 2017 From: gu.lang at gmx.at (Gudrun Lang) Date: Fri, 31 Mar 2017 19:10:55 +0200 Subject: [Histonet] Tissue Fixation In-Reply-To: References: <1730848134.8065268.1490967155655@mail.yahoo.com> <761E2B5697F795489C8710BCC72141FF8E5C6461@ex07.net.ucsf.edu> Message-ID: <000f01d2aa41$c2f22150$48d663f0$@gmx.at> I second the opinion of Joyce. We see such effects in portio-conisations, that are done with a thermo-electrical knife. The surface and the underlying area show a very pink colour in HE. It can also be seen in prostata-chips. IHC on such biopsies shows the effect of an non-stainable edge with a clear cut between positive and negative. https://www.uni-marburg.de/fb20/zahnerhaltk/lehre/Download/Bilder/35_plattenepithelmetaplasie_portio_02212427.jpg I've found this picture, that shows a typical conisation-section. regards Gudrun -----Urspr?ngliche Nachricht----- Von: Weems, Joyce K. via Histonet [mailto:histonet at lists.utsouthwestern.edu] Gesendet: Freitag, 31. M?rz 2017 17:26 An: Morken, Timothy; Rene J Buesa; T H; Histonet at lists.utsouthwestern.edu Betreff: Re: [Histonet] Tissue Fixation Aren't LEEPS done with some sort of electric method that will damage the tissue before it even reaches formalin. I'm not positive but Google it - I believe that might be the problem. j Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax 770-380-8099 Cell joyce.weems at emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph?s Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: Morken, Timothy via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, March 31, 2017 11:07 AM To: Rene J Buesa ; T H Cc: Histonet Subject: Re: [Histonet] Tissue Fixation Tim, I have to agree with Rene that the formalin or time in formalin is obviously not the problem - it has plenty of time in formalin (and who would dilute it anyway?). Handling before formalin must always be determined when problems arise. If the sample sits on a paper towel, gauze etc it does not really degrade faster, rather the tissue may dry out and so fixes by dehydration rather than by formalin. It may be the formalin cannot get into the tissue in those dried out portions of the tissue. However that is just speculation. Since these all seem to be from one particular client, the client is the place to start. The only way to determine the problem is to follow the specimen from start to finish. Can you or someone you trust physically observe the way samples are handled from the time they are taken to the time put in formalin? One issue I always run up against is people saying they do one thing but actually doing another. And they may realize during questioning that they are not doing it right but don't want to admit it. It wastes a lot of time. I've had physicians tell me to ignore the part of the process they are responsible for because they do it right. I just tell them that to be complete we need to follow the process from start to finish, nothing personal, just business. Leaving any part out may lead to not resolving the problem. Probably 99% of the process is just fine, but that 1% is damaging the sample and needs to be illuminated. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Rene J Buesa via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, March 31, 2017 6:33 AM To: T H; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Tissue Fixation What you describe as a possible scenario is absolutely possible.If your PT does not "want to hear" about it, suggest she gets a "hearing aid" or to study something about histotechnology or even better yet, pay attention to what a professional on the subject (you) has to say about it. You would never dare to question her diagnosis, why would she question yours on this subject?Ren? On Friday, March 31, 2017 9:11 AM, T H via Histonet wrote: Good Morning, I have a pathologist that is not happy with the fixation on some of our LEEP specimens. She swears its histology doing something to the specimen to cause the tissue to look unfixed on only "part" of the LEEP specimens (all the same client specimens). She claims we must be diluting our formalin to cause this issue or "something". We mentioned maybe it was on the clients end not placing them in 10% formalin right away, she wouldn't hear of it. Let me give you some back ground on how our process works. Our clients send us all our specimens to us via Overnight FedEx or UPS in 10% formalin they will then they sit in 10% formalin in-house until the processors are started around 3pm and sits an additional 5 hours in 10% formalin on the processor before the processor actually starts. That being said the fixation process has had a pretty good start before we ever even touch it. My question is, and I thought I had read this in the past is, when a specimen is left out prior to fixation and lying on a absorbent surface such as a paper towel, won't the area of the tissue touching the absorbent surface begin the disintegration processes faster in that exact area then the rest of the specimen? Or if you have any other suggestion on what might be happening to only "certain" specimens would be great as well. Thanks for your help! Tim _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SteveM at mcclainlab.com Fri Mar 31 12:53:57 2017 From: SteveM at mcclainlab.com (Steve McClain) Date: Fri, 31 Mar 2017 17:53:57 +0000 Subject: [Histonet] Histonet Digest, Vol 160, Issue 30 Subject: disposable grossing pad for specimen imaging In-Reply-To: References: Message-ID: We use squares of absorbent paper towels to first blot excess fixative and then place on a 2mm thickness piece of art foam. Art foam is used as a background for photography and slicing. By sliding or moving the foam we position the specimen under the camera during specimen photography. We have photographed every specimen since 2004. Send me an email and I will reply w images to illustrate our method. Steve A. McClain, MD > On Mar 31, 2017, at 13:25, "histonet-request at lists.utsouthwestern.edu" wrote: > > Subject: Re: [Histonet] disposable grossing pad From TNMayer at mdanderson.org Fri Mar 31 13:01:19 2017 From: TNMayer at mdanderson.org (Mayer,Toysha N) Date: Fri, 31 Mar 2017 18:01:19 +0000 Subject: [Histonet] disposable grossing pad Message-ID: <47E9B2C01DDDD94881EACD2DC44EBC8839EA2895@D1PWPEXMBX08.mdanderson.edu> We use chux here as well. The cotton in them can bunch up, also they don't lay completely flat. We also use the thin lab mats. Cardinal carries them as does fisher. They can be bought in bulk, have an absorbent side and a fluid resistant side. Since we are on a tight budget here, and students are wasteful, they work well for us. A pack of 30 or so lasts us about two calendar months. We use 1/day and 3/week. There is also a bench top liner that you can purchase. It may come in a roll, and you can just cut what you need. As for disposal, since they are contaminated with specimens, we place ours directly in the biohazard. Any item that may have come in contact with an unprocessed specimen (no blades or glass) goes into the biohazard waste. Hope this helps. Toysha Mayer Message: 1 Date: Thu, 30 Mar 2017 17:42:55 +0000 From: "Terri Braud" To: "'histonet at lists.utsouthwestern.edu'" Subject: Re: [Histonet] disposable grossing pad Message-ID: <48E053DDF6CE074DB6A7414BA05403F812D064 at HRHEX03-HOS.holyredeemer.local> Content-Type: text/plain; charset="us-ascii" I know many, including staff here, that prefer to use "chux". They are very cheap, come in various sizes and adsorption levels, latex-free, with a highly absorbent core and soft, quick-drying coverstock so you don't get lint in your specimens. Use your favorite search engine to look them up. Almost all major Med suppliers carry them. Between specimens, we just toss the old one into red-bag waste. I hope this helps. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal 1. formalin pad for grossing (Wang, Weixi) ---------------------------------------------------------------------- Message: 1 Date: Wed, 29 Mar 2017 17:09:25 +0000 From: "Wang, Weixi" Subject: [Histonet] formalin pad for grossing Dear all Histonetters, It has been a while since I joined the mailing list and I learned a lot here! Now I have a question and would like to get feedbacks regarding the use of formalin in the grossing room. I was instructed to use the formalin-neutralized pads during the grossing. Any other pads or paper, absorbent or not, cannot go to the bio-hazardous waste. But the neutralizer pads are so costly, especially for grossing part, when we need to change very frequently to void the cross contamination between specimen. I would like to know what is the pad/mat/paper you use for grossing, and how do you dispose these formalin contaminated materials. Any feedback is deeply appreciated! Weixi Weixi Wang, Ph.D., HTL(ASCP) Manager | Histology Laboratory St. Joseph's Healthcare System 703 Main Street, Paterson, NJ 07503 Phone: 973.754.3541 | Fax: 973.754.3292 wangweixi at sjhmc.org | www.StJosephsHealth.org *** ------------------------------ _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message may be privileged, confidential, and/or protected from disclosure. This e-mail message may contain protected health information (PHI); dissemination of PHI should comply with applicable federal and state laws. If you are not the intended recipient, or an authorized representative of the intended recipient, any further review, disclosure, use, dissemination, distribution, or copying of this message or any attachment (or the information contained therein) is strictly prohibited. If you think that you have received this e-mail message in error, please notify the sender by return e-mail and delete all references to it and its contents from your systems. From TNMayer at mdanderson.org Fri Mar 31 13:15:24 2017 From: TNMayer at mdanderson.org (Mayer,Toysha N) Date: Fri, 31 Mar 2017 18:15:24 +0000 Subject: [Histonet] Tissue fixation Message-ID: <47E9B2C01DDDD94881EACD2DC44EBC8839EA28B3@D1PWPEXMBX08.mdanderson.edu> I agree with Rene. To discredit the pathologist theory show if all of the specimens from the run are not fixed properly. Then show if it is just the LEEPs. Then show if it is that particular clients specimens? Then onto that client's LEEPs. That should prove your problem lies with the client handling and not the fixation on your end. Cauterization can cause burning of the specimen, but not look unfixed. If it was left out of formalin, autolysis can set in. The situation shows the god-complex some physicians have and the lack of respect they have for their techs. This is why we should all be certified and keep up with continuing education, so that these pathologists will respect us. Ok, I'm going to get off my soapbox now, before I say something ugly. Toysha Mayer ------------------------------ Message: 3 Date: Fri, 31 Mar 2017 12:50:37 +0000 From: T H To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Tissue Fixation Message-ID: Content-Type: text/plain; charset="iso-8859-1" Good Morning, I have a pathologist that is not happy with the fixation on some of our LEEP specimens. She swears its histology doing something to the specimen to cause the tissue to look unfixed on only "part" of the LEEP specimens (all the same client specimens). She claims we must be diluting our formalin to cause this issue or "something". We mentioned maybe it was on the clients end not placing them in 10% formalin right away, she wouldn't hear of it. Let me give you some back ground on how our process works. Our clients send us all our specimens to us via Overnight FedEx or UPS in 10% formalin they will then they sit in 10% formalin in-house until the processors are started around 3pm and sits an additional 5 hours in 10% formalin on the processor before the processor actually starts. That being said the fixation process has had a pretty good start before we ever even touch it. My question is, and I thought I had read this in the past is, when a specimen is left out prior to fixation and lying on a absorbent surface such as a paper towel, won't the area of the tissue touching the absorbent surface begin the disintegration processes faster in that exact area then the rest of the specimen? Or if you have any other suggestion on what might be happening to only "certain" specimens would be great as well. Thanks for your help! Tim ------------------------------ Message: 4 Date: Fri, 31 Mar 2017 13:32:35 +0000 (UTC) From: Rene J Buesa To: T H , "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Tissue Fixation Message-ID: <1730848134.8065268.1490967155655 at mail.yahoo.com> Content-Type: text/plain; charset=UTF-8 What you describe as a possible scenario is absolutely possible.If your PT does not "want to hear" about it, suggest she gets a "hearing aid" or to study something about histotechnology or even better yet, pay attention to what a professional on the subject (you) has to say about it. You would never dare to question her diagnosis, why would she question yours on this subject?Ren? On Friday, March 31, 2017 9:11 AM, T H via Histonet wrote: Good Morning, I have a pathologist that is not happy with the fixation on some of our LEEP specimens.? She swears its histology doing something to the specimen to cause the tissue to look unfixed on only "part" of the LEEP specimens (all the same client specimens).? She claims we must be diluting our formalin to cause this issue or "something".? We mentioned maybe it was on the clients end not placing them in 10% formalin right away, she wouldn't hear of it. Let me give you some back ground on how our process works.? Our clients send us all our specimens to us via Overnight FedEx or UPS in 10% formalin they will then they sit in 10% formalin in-house until the processors are started around 3pm and sits an additional 5 hours in 10% formalin on the processor before the processor actually starts.? That being said the fixation process has had a pretty good start before we ever even touch it. My question is, and I thought I had read this in the past is, when a specimen is left out prior to fixation and lying on a absorbent surface such as a paper towel, won't the area of the tissue touching the absorbent surface begin the disintegration processes faster in that exact area then the rest of the specimen?? Or if you have any other suggestion on what might be happening to only "certain" specimens would be great as well. Thanks for your help! Tim _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Fri, 31 Mar 2017 13:57:30 +0000 (UTC) From: Paula Keene Pierce To: Rene J Buesa , Histonet Histonet Subject: Re: [Histonet] Tissue Fixation Message-ID: <372734167.8016960.1490968650877 at mail.yahoo.com> Content-Type: text/plain; charset=UTF-8 Bravo Rene!?Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953FAX 405-759-7513www.excaliburpathology.com From: Rene J Buesa via Histonet To: T H ; "histonet at lists.utsouthwestern.edu" Sent: Friday, March 31, 2017 8:44 AM Subject: Re: [Histonet] Tissue Fixation What you describe as a possible scenario is absolutely possible.If your PT does not "want to hear" about it, suggest she gets a "hearing aid" or to study something about histotechnology or even better yet, pay attention to what a professional on the subject (you) has to say about it. You would never dare to question her diagnosis, why would she question yours on this subject?Ren? ? ? On Friday, March 31, 2017 9:11 AM, T H via Histonet wrote: Good Morning, I have a pathologist that is not happy with the fixation on some of our LEEP specimens.? She swears its histology doing something to the specimen to cause the tissue to look unfixed on only "part" of the LEEP specimens (all the same client specimens).? She claims we must be diluting our formalin to cause this issue or "something".? We mentioned maybe it was on the clients end not placing them in 10% formalin right away, she wouldn't hear of it. Let me give you some back ground on how our process works.? Our clients send us all our specimens to us via Overnight FedEx or UPS in 10% formalin they will then they sit in 10% formalin in-house until the processors are started around 3pm and sits an additional 5 hours in 10% formalin on the processor before the processor actually starts.? That being said the fixation process has had a pretty good start before we ever even touch it. My question is, and I thought I had read this in the past is, when a specimen is left out prior to fixation and lying on a absorbent surface such as a paper towel, won't the area of the tissue touching the absorbent surface begin the disintegration processes faster in that exact area then the rest of the specimen?? Or if you have any other suggestion on what might be happening to only "certain" specimens would be great as well. Thanks for your help! Tim Message: 6 Date: Fri, 31 Mar 2017 15:06:53 +0000 From: "Morken, Timothy" To: Rene J Buesa , T H Cc: Histonet Subject: Re: [Histonet] Tissue Fixation Message-ID: <761E2B5697F795489C8710BCC72141FF8E5C6461 at ex07.net.ucsf.edu> Content-Type: text/plain; charset="utf-8" Tim, I have to agree with Rene that the formalin or time in formalin is obviously not the problem - it has plenty of time in formalin (and who would dilute it anyway?). Handling before formalin must always be determined when problems arise. If the sample sits on a paper towel, gauze etc it does not really degrade faster, rather the tissue may dry out and so fixes by dehydration rather than by formalin. It may be the formalin cannot get into the tissue in those dried out portions of the tissue. However that is just speculation. Since these all seem to be from one particular client, the client is the place to start. The only way to determine the problem is to follow the specimen from start to finish. Can you or someone you trust physically observe the way samples are handled from the time they are taken to the time put in formalin? One issue I always run up against is people saying they do one thing but actually doing another. And they may realize during questioning that they are not doing it right but don't want to admit it. It wastes a lot of time. I've had physicians tell me to ignore the part of the process they are responsible for because they do it right. I just tell them that to be complete we need to follow the process from start to finish, nothing personal, just business. Leaving any part out may lead to not resolving the problem. Probably 99% of the process is just fine, but that 1% is damaging the sample and needs to be illuminated. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center Content-Type: text/plain; charset="utf-8" Aren't LEEPS done with some sort of electric method that will damage the tissue before it even reaches formalin. I'm not positive but Google it - I believe that might be the problem. j Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax 770-380-8099 Cell joyce.weems at emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 ________________________________ The information contained in this e-mail message may be privileged, confidential, and/or protected from disclosure. This e-mail message may contain protected health information (PHI); dissemination of PHI should comply with applicable federal and state laws. If you are not the intended recipient, or an authorized representative of the intended recipient, any further review, disclosure, use, dissemination, distribution, or copying of this message or any attachment (or the information contained therein) is strictly prohibited. If you think that you have received this e-mail message in error, please notify the sender by return e-mail and delete all references to it and its contents from your systems. From rsrichmond at gmail.com Fri Mar 31 13:25:21 2017 From: rsrichmond at gmail.com (Bob Richmond) Date: Fri, 31 Mar 2017 13:25:21 -0500 Subject: [Histonet] Tissue fixation Message-ID: Tim describes a problem "I have a pathologist that is not happy with the fixation on some of our LEEP specimens." LEEP specimens are inherently crappy, because of the cautery used to obtain them, and the resulting cautery artifact in the specimens. In the last few years they've turned the voltage down, and that's helped some - and perhaps this particular clinic hasn't turned the voltage down. I wonder how familiar your pathologist is with the whole process - the sort of homely detail not taught in pathology residency, and the sort of detail I often go out and research on my own. It's helpful to all to know that the clinical stakes here are not high - we do report margins on cervical LEEP specimens (when we can figure them out), but clinical outcomes apparently don't have much to do with whether margins are positive for dysplasia or not. It's impossible to get india ink to stick to the cauterized gross margins, but the cautery artifact is painfully obvious under the microscope anyway. Bob Richmond Samurai Pathologist Maryville TN From Nancy.Stedman at buschgardens.com Fri Mar 31 13:39:46 2017 From: Nancy.Stedman at buschgardens.com (Stedman, Nancy) Date: Fri, 31 Mar 2017 18:39:46 +0000 Subject: [Histonet] [EXTERNAL] Re: Tissue fixation In-Reply-To: <47E9B2C01DDDD94881EACD2DC44EBC8839EA28B3@D1PWPEXMBX08.mdanderson.edu> References: <47E9B2C01DDDD94881EACD2DC44EBC8839EA28B3@D1PWPEXMBX08.mdanderson.edu> Message-ID: <18D791D4EE07BC41BF05F8EF3CCCDE247FDE0714@SEAP4002.nam.int.local> I would bet there are quite a few pathologists on this mailing list (like me) who are here because we respect histo techs and know you have valuable information to offer! I agree, cautery artifact is pretty easy to tell from poor fixation, so it should be easy to determine if that is the problem. Sometimes, specimens that are submitted smashed in a cassette may have poor fixation of the tissue in contact with the cassette. Or if they are submitted sponged and floating, some tissue may not be fully immersed in formalin. Also, crush artifact may resemble poor fixation, so maybe the clinical team is not handling the tissue gently enough? I read veterinary specimens; we don't do LEEPs so I don't know if any of these scenarios are possible, but they are things I see frequently. -Nancy Stedman -----Original Message----- From: Mayer,Toysha N via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, March 31, 2017 2:15 PM To: 'histonet at lists.utsouthwestern.edu' Subject: [EXTERNAL] Re: [Histonet] Tissue fixation I agree with Rene. To discredit the pathologist theory show if all of the specimens from the run are not fixed properly. Then show if it is just the LEEPs. Then show if it is that particular clients specimens? Then onto that client's LEEPs. That should prove your problem lies with the client handling and not the fixation on your end. Cauterization can cause burning of the specimen, but not look unfixed. If it was left out of formalin, autolysis can set in. The situation shows the god-complex some physicians have and the lack of respect they have for their techs. This is why we should all be certified and keep up with continuing education, so that these pathologists will respect us. Ok, I'm going to get off my soapbox now, before I say something ugly. Toysha Mayer ------------------------------ Message: 3 Date: Fri, 31 Mar 2017 12:50:37 +0000 From: T H To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Tissue Fixation Message-ID: Content-Type: text/plain; charset="iso-8859-1" Good Morning, I have a pathologist that is not happy with the fixation on some of our LEEP specimens. She swears its histology doing something to the specimen to cause the tissue to look unfixed on only "part" of the LEEP specimens (all the same client specimens). She claims we must be diluting our formalin to cause this issue or "something". We mentioned maybe it was on the clients end not placing them in 10% formalin right away, she wouldn't hear of it. Let me give you some back ground on how our process works. Our clients send us all our specimens to us via Overnight FedEx or UPS in 10% formalin they will then they sit in 10% formalin in-house until the processors are started around 3pm and sits an additional 5 hours in 10% formalin on the processor before the processor actually starts. That being said the fixation process has had a pretty good start before we ever even touch it. My question is, and I thought I had read this in the past is, when a specimen is left out prior to fixation and lying on a absorbent surface such as a paper towel, won't the area of the tissue touching the absorbent surface begin the disintegration processes faster in that exact area then the rest of the specimen? Or if you have any other suggestion on what might be happening to only "certain" specimens would be great as well. Thanks for your help! Tim ------------------------------ Message: 4 Date: Fri, 31 Mar 2017 13:32:35 +0000 (UTC) From: Rene J Buesa To: T H , "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Tissue Fixation Message-ID: <1730848134.8065268.1490967155655 at mail.yahoo.com> Content-Type: text/plain; charset=UTF-8 What you describe as a possible scenario is absolutely possible.If your PT does not "want to hear" about it, suggest she gets a "hearing aid" or to study something about histotechnology or even better yet, pay attention to what a professional on the subject (you) has to say about it. You would never dare to question her diagnosis, why would she question yours on this subject?Ren? On Friday, March 31, 2017 9:11 AM, T H via Histonet wrote: Good Morning, I have a pathologist that is not happy with the fixation on some of our LEEP specimens.? She swears its histology doing something to the specimen to cause the tissue to look unfixed on only "part" of the LEEP specimens (all the same client specimens).? She claims we must be diluting our formalin to cause this issue or "something".? We mentioned maybe it was on the clients end not placing them in 10% formalin right away, she wouldn't hear of it. Let me give you some back ground on how our process works.? Our clients send us all our specimens to us via Overnight FedEx or UPS in 10% formalin they will then they sit in 10% formalin in-house until the processors are started around 3pm and sits an additional 5 hours in 10% formalin on the processor before the processor actually starts.? That being said the fixation process has had a pretty good start before we ever even touch it. My question is, and I thought I had read this in the past is, when a specimen is left out prior to fixation and lying on a absorbent surface such as a paper towel, won't the area of the tissue touching the absorbent surface begin the disintegration processes faster in that exact area then the rest of the specimen?? Or if you have any other suggestion on what might be happening to only "certain" specimens would be great as well. Thanks for your help! Tim _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Fri, 31 Mar 2017 13:57:30 +0000 (UTC) From: Paula Keene Pierce To: Rene J Buesa , Histonet Histonet Subject: Re: [Histonet] Tissue Fixation Message-ID: <372734167.8016960.1490968650877 at mail.yahoo.com> Content-Type: text/plain; charset=UTF-8 Bravo Rene!?Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953FAX 405-759-7513www.excaliburpathology.com From: Rene J Buesa via Histonet To: T H ; "histonet at lists.utsouthwestern.edu" Sent: Friday, March 31, 2017 8:44 AM Subject: Re: [Histonet] Tissue Fixation What you describe as a possible scenario is absolutely possible.If your PT does not "want to hear" about it, suggest she gets a "hearing aid" or to study something about histotechnology or even better yet, pay attention to what a professional on the subject (you) has to say about it. You would never dare to question her diagnosis, why would she question yours on this subject?Ren? ? ? On Friday, March 31, 2017 9:11 AM, T H via Histonet wrote: Good Morning, I have a pathologist that is not happy with the fixation on some of our LEEP specimens.? She swears its histology doing something to the specimen to cause the tissue to look unfixed on only "part" of the LEEP specimens (all the same client specimens).? She claims we must be diluting our formalin to cause this issue or "something".? We mentioned maybe it was on the clients end not placing them in 10% formalin right away, she wouldn't hear of it. Let me give you some back ground on how our process works.? Our clients send us all our specimens to us via Overnight FedEx or UPS in 10% formalin they will then they sit in 10% formalin in-house until the processors are started around 3pm and sits an additional 5 hours in 10% formalin on the processor before the processor actually starts.? That being said the fixation process has had a pretty good start before we ever even touch it. My question is, and I thought I had read this in the past is, when a specimen is left out prior to fixation and lying on a absorbent surface such as a paper towel, won't the area of the tissue touching the absorbent surface begin the disintegration processes faster in that exact area then the rest of the specimen?? Or if you have any other suggestion on what might be happening to only "certain" specimens would be great as well. Thanks for your help! Tim Message: 6 Date: Fri, 31 Mar 2017 15:06:53 +0000 From: "Morken, Timothy" To: Rene J Buesa , T H Cc: Histonet Subject: Re: [Histonet] Tissue Fixation Message-ID: <761E2B5697F795489C8710BCC72141FF8E5C6461 at ex07.net.ucsf.edu> Content-Type: text/plain; charset="utf-8" Tim, I have to agree with Rene that the formalin or time in formalin is obviously not the problem - it has plenty of time in formalin (and who would dilute it anyway?). Handling before formalin must always be determined when problems arise. If the sample sits on a paper towel, gauze etc it does not really degrade faster, rather the tissue may dry out and so fixes by dehydration rather than by formalin. It may be the formalin cannot get into the tissue in those dried out portions of the tissue. However that is just speculation. Since these all seem to be from one particular client, the client is the place to start. The only way to determine the problem is to follow the specimen from start to finish. Can you or someone you trust physically observe the way samples are handled from the time they are taken to the time put in formalin? One issue I always run up against is people saying they do one thing but actually doing another. And they may realize during questioning that they are not doing it right but don't want to admit it. It wastes a lot of time. I've had physicians tell me to ignore the part of the process they are responsible for because they do it right. I just tell them that to be complete we need to follow the process from start to finish, nothing personal, just business. Leaving any part out may lead to not resolving the problem. Probably 99% of the process is just fine, but that 1% is damaging the sample and needs to be illuminated. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center Content-Type: text/plain; charset="utf-8" Aren't LEEPS done with some sort of electric method that will damage the tissue before it even reaches formalin. I'm not positive but Google it - I believe that might be the problem. j Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax 770-380-8099 Cell joyce.weems at emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 ________________________________ The information contained in this e-mail message may be privileged, confidential, and/or protected from disclosure. This e-mail message may contain protected health information (PHI); dissemination of PHI should comply with applicable federal and state laws. If you are not the intended recipient, or an authorized representative of the intended recipient, any further review, disclosure, use, dissemination, distribution, or copying of this message or any attachment (or the information contained therein) is strictly prohibited. If you think that you have received this e-mail message in error, please notify the sender by return e-mail and delete all references to it and its contents from your systems. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nelsonrnch at verizon.net Fri Mar 31 13:58:35 2017 From: nelsonrnch at verizon.net (Patti Nelson - PNP Lab Consultant) Date: Fri, 31 Mar 2017 14:58:35 -0400 Subject: [Histonet] Survey!!!!!! Message-ID: <15b25bbc879-4891-5749@webprd-a21.mail.aol.com> Hi Everyone, I just wanted to get everyone's opinion. If you had to chose between buying a Auto Side Stainer or Auto Slide Cover Slipper, which one would you chose? Lets say your volume was around 60 to 80 blocks a day and you worked for a GI Lab. Everyone's input would be greatly appreciated. Sincerely, PATTI NELSON H.T.(ASCP) PNP LABORATORY CONSULTANTS SUPERVISOR DGC/ZADEH LABS PO BOX 412 CABAZON, CA. 92230 909-841-9761 nelsonrnch at verizon.net CONFIDENTIALITY NOTICE:This message and any included attachments are from Patti Nelson, PNP Laboratory Consultants and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender ofthe delivery error by e-mail or you may call 909-841-9761. From wbenton at cua.md Fri Mar 31 14:39:07 2017 From: wbenton at cua.md (Walter Benton) Date: Fri, 31 Mar 2017 19:39:07 +0000 Subject: [Histonet] Survey!!!!!! In-Reply-To: <15b25bbc879-4891-5749@webprd-a21.mail.aol.com> References: <15b25bbc879-4891-5749@webprd-a21.mail.aol.com> Message-ID: <1490989121876.97250@cua.md> How many slides are you producing from that number of blocks? How many techs do you have that are able to hand stain and/or hand coverslip? Walter Benton HT(ASCP)QIHC Lab Operations Manager Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) Chesapeakeurology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. ________________________________________ From: Patti Nelson - PNP Lab Consultant via Histonet Sent: Friday, March 31, 2017 2:58 PM To: Histonet at lists.utsouthwestern.edu Subject: [Histonet] Survey!!!!!! Hi Everyone, I just wanted to get everyone's opinion. If you had to chose between buying a Auto Side Stainer or Auto Slide Cover Slipper, which one would you chose? Lets say your volume was around 60 to 80 blocks a day and you worked for a GI Lab. Everyone's input would be greatly appreciated. Sincerely, PATTI NELSON H.T.(ASCP) PNP LABORATORY CONSULTANTS SUPERVISOR DGC/ZADEH LABS PO BOX 412 CABAZON, CA. 92230 909-841-9761 nelsonrnch at verizon.net CONFIDENTIALITY NOTICE:This message and any included attachments are from Patti Nelson, PNP Laboratory Consultants and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender ofthe delivery error by e-mail or you may call 909-841-9761. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From nelsonrnch at verizon.net Fri Mar 31 14:50:24 2017 From: nelsonrnch at verizon.net (Patti Nelson - PNP Lab Consultant) Date: Fri, 31 Mar 2017 15:50:24 -0400 Subject: [Histonet] Survey!!!!!! In-Reply-To: <1490989121876.97250@cua.md> Message-ID: <15b25eb392e-4891-5a4c@webprd-a21.mail.aol.com> Walter the staff consist of 1 Histotech and 1 lab assistant. On an average there will be 2 special stains per block. Hope that helps. Sincerely, PATTI NELSON H.T.(ASCP) PNP LABORATORY CONSULTANTS SUPERVISOR DGC/ZADEH LABS PO BOX 412 CABAZON, CA. 92230 909-841-9761 nelsonrnch at verizon.net CONFIDENTIALITY NOTICE:This message and any included attachments are from Patti Nelson, PNP Laboratory Consultants and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender ofthe delivery error by e-mail or you may call 909-841-9761. -----Original Message----- From: Walter Benton To: Patti Nelson - PNP Lab Consultant ; Histonet Sent: Fri, Mar 31, 2017 12:39 pm Subject: Re: [Histonet] Survey!!!!!! How many slides are you producing from that number of blocks? How many techs do you have that are able to hand stain and/or hand coverslip? Walter Benton HT(ASCP)QIHC Lab Operations Manager Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) Chesapeakeurology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. ________________________________________ From: Patti Nelson - PNP Lab Consultant via Histonet Sent: Friday, March 31, 2017 2:58 PM To: Histonet at lists.utsouthwestern.edu Subject: [Histonet] Survey!!!!!! Hi Everyone, I just wanted to get everyone's opinion. If you had to chose between buying a Auto Side Stainer or Auto Slide Cover Slipper, which one would you chose? Lets say your volume was around 60 to 80 blocks a day and you worked for a GI Lab. Everyone's input would be greatly appreciated. Sincerely, PATTI NELSON H.T.(ASCP) PNP LABORATORY CONSULTANTS SUPERVISOR DGC/ZADEH LABS PO BOX 412 CABAZON, CA. 92230 909-841-9761 nelsonrnch at verizon.net CONFIDENTIALITY NOTICE:This message and any included attachments are from Patti Nelson, PNP Laboratory Consultants and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender ofthe delivery error by e-mail or you may call 909-841-9761. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From liz at premierlab.com Fri Mar 31 14:55:08 2017 From: liz at premierlab.com (Elizabeth Chlipala) Date: Fri, 31 Mar 2017 13:55:08 -0600 Subject: [Histonet] [EXTERNAL] Re: Tissue fixation In-Reply-To: <18D791D4EE07BC41BF05F8EF3CCCDE247FDE0714@SEAP4002.nam.int.local> References: <47E9B2C01DDDD94881EACD2DC44EBC8839EA28B3@D1PWPEXMBX08.mdanderson.edu> <18D791D4EE07BC41BF05F8EF3CCCDE247FDE0714@SEAP4002.nam.int.local> Message-ID: <14E2C6176416974295479C64A11CB9AE0302C0353EC2@SBS2K8.premierlab.local> I'm going to give my two cents here. I think you need to look closely at how you handle these samples. First of all leeps are not small samples therefore just because the leep has been placed in fixative upon removal and then sits in fixative until its processed. Unless the sample is grossed into smaller pieces only then it will adequately fix. Electro cautery instruments function to do two things, they cut and then cauterize - there is always a thermal defect associated with the process or thermal spread, this is clearly evident on the H&E slides. The process is essentially coagulating the tissue proteins. The thermal spread and char that is associated with this process varies dependent upon the instrument and setting that is being used. This process may decrease fixative penetration so it's important that these samples are grossed in as soon as possible. Trimming the tissue will allow for better penetration of the fixative and good fixation requires at least 4 to 6 hours in formalin AFTER the tissue has been trimmed in. The artifact that the pathologist is seeing might not be related to the amount of time the sample sits in formalin it might be due to the amount of time the samples sits in formalin prior to being grossed in. If I had this issue I would start tracking the different times associated with these samples and possibly the instrument and setting used (if you are able to get that info). 1. Time to fixative - cold ischemic time 2. Time to when the sample is grossed in 3. Time in formalin once the sample is grossed in Good Luck I hope this helps. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: Stedman, Nancy via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, March 31, 2017 12:40 PM To: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] [EXTERNAL] Re: Tissue fixation I would bet there are quite a few pathologists on this mailing list (like me) who are here because we respect histo techs and know you have valuable information to offer! I agree, cautery artifact is pretty easy to tell from poor fixation, so it should be easy to determine if that is the problem. Sometimes, specimens that are submitted smashed in a cassette may have poor fixation of the tissue in contact with the cassette. Or if they are submitted sponged and floating, some tissue may not be fully immersed in formalin. Also, crush artifact may resemble poor fixation, so maybe the clinical team is not handling the tissue gently enough? I read veterinary specimens; we don't do LEEPs so I don't know if any of these scenarios are possible, but they are things I see frequently. -Nancy Stedman -----Original Message----- From: Mayer,Toysha N via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, March 31, 2017 2:15 PM To: 'histonet at lists.utsouthwestern.edu' Subject: [EXTERNAL] Re: [Histonet] Tissue fixation I agree with Rene. To discredit the pathologist theory show if all of the specimens from the run are not fixed properly. Then show if it is just the LEEPs. Then show if it is that particular clients specimens? Then onto that client's LEEPs. That should prove your problem lies with the client handling and not the fixation on your end. Cauterization can cause burning of the specimen, but not look unfixed. If it was left out of formalin, autolysis can set in. The situation shows the god-complex some physicians have and the lack of respect they have for their techs. This is why we should all be certified and keep up with continuing education, so that these pathologists will respect us. Ok, I'm going to get off my soapbox now, before I say something ugly. Toysha Mayer ------------------------------ Message: 3 Date: Fri, 31 Mar 2017 12:50:37 +0000 From: T H To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Tissue Fixation Message-ID: Content-Type: text/plain; charset="iso-8859-1" Good Morning, I have a pathologist that is not happy with the fixation on some of our LEEP specimens. She swears its histology doing something to the specimen to cause the tissue to look unfixed on only "part" of the LEEP specimens (all the same client specimens). She claims we must be diluting our formalin to cause this issue or "something". We mentioned maybe it was on the clients end not placing them in 10% formalin right away, she wouldn't hear of it. Let me give you some back ground on how our process works. Our clients send us all our specimens to us via Overnight FedEx or UPS in 10% formalin they will then they sit in 10% formalin in-house until the processors are started around 3pm and sits an additional 5 hours in 10% formalin on the processor before the processor actually starts. That being said the fixation process has had a pretty good start before we ever even touch it. My question is, and I thought I had read this in the past is, when a specimen is left out prior to fixation and lying on a absorbent surface such as a paper towel, won't the area of the tissue touching the absorbent surface begin the disintegration processes faster in that exact area then the rest of the specimen? Or if you have any other suggestion on what might be happening to only "certain" specimens would be great as well. Thanks for your help! Tim ------------------------------ Message: 4 Date: Fri, 31 Mar 2017 13:32:35 +0000 (UTC) From: Rene J Buesa To: T H , "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Tissue Fixation Message-ID: <1730848134.8065268.1490967155655 at mail.yahoo.com> Content-Type: text/plain; charset=UTF-8 What you describe as a possible scenario is absolutely possible.If your PT does not "want to hear" about it, suggest she gets a "hearing aid" or to study something about histotechnology or even better yet, pay attention to what a professional on the subject (you) has to say about it. You would never dare to question her diagnosis, why would she question yours on this subject?Ren? On Friday, March 31, 2017 9:11 AM, T H via Histonet wrote: Good Morning, I have a pathologist that is not happy with the fixation on some of our LEEP specimens.? She swears its histology doing something to the specimen to cause the tissue to look unfixed on only "part" of the LEEP specimens (all the same client specimens).? She claims we must be diluting our formalin to cause this issue or "something".? We mentioned maybe it was on the clients end not placing them in 10% formalin right away, she wouldn't hear of it. Let me give you some back ground on how our process works.? Our clients send us all our specimens to us via Overnight FedEx or UPS in 10% formalin they will then they sit in 10% formalin in-house until the processors are started around 3pm and sits an additional 5 hours in 10% formalin on the processor before the processor actually starts.? That being said the fixation process has had a pretty good start before we ever even touch it. My question is, and I thought I had read this in the past is, when a specimen is left out prior to fixation and lying on a absorbent surface such as a paper towel, won't the area of the tissue touching the absorbent surface begin the disintegration processes faster in that exact area then the rest of the specimen?? Or if you have any other suggestion on what might be happening to only "certain" specimens would be great as well. Thanks for your help! Tim _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Fri, 31 Mar 2017 13:57:30 +0000 (UTC) From: Paula Keene Pierce To: Rene J Buesa , Histonet Histonet Subject: Re: [Histonet] Tissue Fixation Message-ID: <372734167.8016960.1490968650877 at mail.yahoo.com> Content-Type: text/plain; charset=UTF-8 Bravo Rene!?Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953FAX 405-759-7513www.excaliburpathology.com From: Rene J Buesa via Histonet To: T H ; "histonet at lists.utsouthwestern.edu" Sent: Friday, March 31, 2017 8:44 AM Subject: Re: [Histonet] Tissue Fixation What you describe as a possible scenario is absolutely possible.If your PT does not "want to hear" about it, suggest she gets a "hearing aid" or to study something about histotechnology or even better yet, pay attention to what a professional on the subject (you) has to say about it. You would never dare to question her diagnosis, why would she question yours on this subject?Ren? ? ? On Friday, March 31, 2017 9:11 AM, T H via Histonet wrote: Good Morning, I have a pathologist that is not happy with the fixation on some of our LEEP specimens.? She swears its histology doing something to the specimen to cause the tissue to look unfixed on only "part" of the LEEP specimens (all the same client specimens).? She claims we must be diluting our formalin to cause this issue or "something".? We mentioned maybe it was on the clients end not placing them in 10% formalin right away, she wouldn't hear of it. Let me give you some back ground on how our process works.? Our clients send us all our specimens to us via Overnight FedEx or UPS in 10% formalin they will then they sit in 10% formalin in-house until the processors are started around 3pm and sits an additional 5 hours in 10% formalin on the processor before the processor actually starts.? That being said the fixation process has had a pretty good start before we ever even touch it. My question is, and I thought I had read this in the past is, when a specimen is left out prior to fixation and lying on a absorbent surface such as a paper towel, won't the area of the tissue touching the absorbent surface begin the disintegration processes faster in that exact area then the rest of the specimen?? Or if you have any other suggestion on what might be happening to only "certain" specimens would be great as well. Thanks for your help! Tim Message: 6 Date: Fri, 31 Mar 2017 15:06:53 +0000 From: "Morken, Timothy" To: Rene J Buesa , T H Cc: Histonet Subject: Re: [Histonet] Tissue Fixation Message-ID: <761E2B5697F795489C8710BCC72141FF8E5C6461 at ex07.net.ucsf.edu> Content-Type: text/plain; charset="utf-8" Tim, I have to agree with Rene that the formalin or time in formalin is obviously not the problem - it has plenty of time in formalin (and who would dilute it anyway?). Handling before formalin must always be determined when problems arise. If the sample sits on a paper towel, gauze etc it does not really degrade faster, rather the tissue may dry out and so fixes by dehydration rather than by formalin. It may be the formalin cannot get into the tissue in those dried out portions of the tissue. However that is just speculation. Since these all seem to be from one particular client, the client is the place to start. The only way to determine the problem is to follow the specimen from start to finish. Can you or someone you trust physically observe the way samples are handled from the time they are taken to the time put in formalin? One issue I always run up against is people saying they do one thing but actually doing another. And they may realize during questioning that they are not doing it right but don't want to admit it. It wastes a lot of time. I've had physicians tell me to ignore the part of the process they are responsible for because they do it right. I just tell them that to be complete we need to follow the process from start to finish, nothing personal, just business. Leaving any part out may lead to not resolving the problem. Probably 99% of the process is just fine, but that 1% is damaging the sample and needs to be illuminated. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center Content-Type: text/plain; charset="utf-8" Aren't LEEPS done with some sort of electric method that will damage the tissue before it even reaches formalin. I'm not positive but Google it - I believe that might be the problem. j Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax 770-380-8099 Cell joyce.weems at emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 ________________________________ The information contained in this e-mail message may be privileged, confidential, and/or protected from disclosure. This e-mail message may contain protected health information (PHI); dissemination of PHI should comply with applicable federal and state laws. If you are not the intended recipient, or an authorized representative of the intended recipient, any further review, disclosure, use, dissemination, distribution, or copying of this message or any attachment (or the information contained therein) is strictly prohibited. If you think that you have received this e-mail message in error, please notify the sender by return e-mail and delete all references to it and its contents from your systems. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the Symantec Email Security.cloud service. For more information please visit http://www.symanteccloud.com ______________________________________________________________________ From mdpraet at gmail.com Fri Mar 31 15:05:25 2017 From: mdpraet at gmail.com (Mequita Praet) Date: Fri, 31 Mar 2017 16:05:25 -0400 Subject: [Histonet] Auto Stainer vs Auto Coverslipper Message-ID: Hands down the Automatic Stainer preferred over the coverslipper. You can do H & E & you can do the AB/PAS as well on it. No standing there hand dipping. You can be doing something else and come back and take them off to sit and hand coverslipp. Email me personally if you want to know which Automatic Stainer I have many years of experience with. It is a work horse. With yearly maintenance hardly ever breaks down. Mequita Praet, HTL(ASCP)SLS Mdpraet at gmail.com From wbenton at cua.md Fri Mar 31 15:38:41 2017 From: wbenton at cua.md (Walter Benton) Date: Fri, 31 Mar 2017 20:38:41 +0000 Subject: [Histonet] Survey!!!!!! In-Reply-To: <15b25eb392e-4891-5a4c@webprd-a21.mail.aol.com> References: <1490989121876.97250@cua.md>, <15b25eb392e-4891-5a4c@webprd-a21.mail.aol.com> Message-ID: <1490992737943.33643@cua.md> ?Patti, If you are able to incorporate the special stains along with the H&E on the automated stainer, I think that would be the best option. Based on your information you may have 120-180 slides to coverslip by hand each day. Walter Benton HT(ASCP)QIHC Lab Operations Manager Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) Chesapeakeurology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. ________________________________ From: Patti Nelson - PNP Lab Consultant Sent: Friday, March 31, 2017 3:50 PM To: Walter Benton; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Survey!!!!!! Walter the staff consist of 1 Histotech and 1 lab assistant. On an average there will be 2 special stains per block. Hope that helps. Sincerely, PATTI NELSON H.T.(ASCP) PNP LABORATORY CONSULTANTS SUPERVISOR DGC/ZADEH LABS PO BOX 412 CABAZON, CA. 92230 909-841-9761 nelsonrnch at verizon.net CONFIDENTIALITY NOTICE:This message and any included attachments are from Patti Nelson, PNP Laboratory Consultants and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender ofthe delivery error by e-mail or you may call 909-841-9761. -----Original Message----- From: Walter Benton To: Patti Nelson - PNP Lab Consultant ; Histonet Sent: Fri, Mar 31, 2017 12:39 pm Subject: Re: [Histonet] Survey!!!!!! How many slides are you producing from that number of blocks? How many techs do you have that are able to hand stain and/or hand coverslip? Walter Benton HT(ASCP)QIHC Lab Operations Manager Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) Chesapeakeurology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. ________________________________________ From: Patti Nelson - PNP Lab Consultant via Histonet > Sent: Friday, March 31, 2017 2:58 PM To: Histonet at lists.utsouthwestern.edu Subject: [Histonet] Survey!!!!!! Hi Everyone, I just wanted to get everyone's opinion. If you had to chose between buying a Auto Side Stainer or Auto Slide Cover Slipper, which one would you chose? Lets say your volume was around 60 to 80 blocks a day and you worked for a GI Lab. Everyone's input would be greatly appreciated. Sincerely, PATTI NELSON H.T.(ASCP) PNP LABORATORY CONSULTANTS SUPERVISOR DGC/ZADEH LABS PO BOX 412 CABAZON, CA. 92230 909-841-9761 nelsonrnch at verizon.net CONFIDENTIALITY NOTICE:This message and any included attachments are from Patti Nelson, PNP Laboratory Consultants and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender ofthe delivery error by e-mail or you may call 909-841-9761. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From craigak12 at gmail.com Fri Mar 31 16:34:51 2017 From: craigak12 at gmail.com (J B) Date: Fri, 31 Mar 2017 21:34:51 +0000 Subject: [Histonet] Survey!!!!!! In-Reply-To: <1490992737943.33643@cua.md> References: <1490989121876.97250@cua.md> <15b25eb392e-4891-5a4c@webprd-a21.mail.aol.com> <1490992737943.33643@cua.md> Message-ID: Stainer for sure. JB On Fri, Mar 31, 2017, 2:05 PM Walter Benton via Histonet < histonet at lists.utsouthwestern.edu> wrote: > ?Patti, > > > If you are able to incorporate the special stains along with the H&E on > the automated stainer, I think that would be the best option. Based on your > information you may have 120-180 slides to coverslip by hand each day. > > > Walter Benton HT(ASCP)QIHC > Lab Operations Manager > Chesapeake Urology Associates > 806 Landmark Drive, Suite 127 > Glen Burnie, MD 21061 > 443-471-5850 (Direct) > 410-768-5961 (Lab) > 410-768-5965 (Fax) > Chesapeakeurology.com > > Voted a Best Place to Work by > Baltimore and Modern Healthcare > Magazines. > ________________________________ > From: Patti Nelson - PNP Lab Consultant > Sent: Friday, March 31, 2017 3:50 PM > To: Walter Benton; histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] Survey!!!!!! > > Walter the staff consist of 1 Histotech and 1 lab assistant. On an average > there will be 2 special stains per block. Hope that helps. > > Sincerely, > > PATTI NELSON H.T.(ASCP) > PNP LABORATORY CONSULTANTS > SUPERVISOR DGC/ZADEH LABS > PO BOX 412 > CABAZON, CA. 92230 > 909-841-9761 > nelsonrnch at verizon.net > CONFIDENTIALITY NOTICE:This message and any included attachments are from > Patti Nelson, PNP Laboratory Consultants and are intended only for the > addressee. The information contained in this message is confidential and > may contain privileged, confidential, proprietary and/or exemption from > disclosure under applicable law. Unauthorized forwarding, printing, > copying, distribution, or use of such information is strictly prohibited > and may be unlawful. If you are not the addressee, please promptly delete > this message and notify the sender ofthe delivery error by e-mail or you > may call 909-841-9761. > > > -----Original Message----- > From: Walter Benton > To: Patti Nelson - PNP Lab Consultant ; Histonet < > histonet at lists.utsouthwestern.edu> > Sent: Fri, Mar 31, 2017 12:39 pm > Subject: Re: [Histonet] Survey!!!!!! > > How many slides are you producing from that number of blocks? How many > techs do you have that are able to hand stain and/or hand coverslip? > > Walter Benton HT(ASCP)QIHC > Lab Operations Manager > Chesapeake Urology Associates > 806 Landmark Drive, Suite 127 > Glen Burnie, MD 21061 > 443-471-5850 (Direct) > 410-768-5961 (Lab) > 410-768-5965 (Fax) > Chesapeakeurology.com > > Voted a Best Place to Work by > Baltimore and Modern Healthcare > Magazines. > ________________________________________ > From: Patti Nelson - PNP Lab Consultant via Histonet < > histonet at lists.utsouthwestern.edu >> > Sent: Friday, March 31, 2017 2:58 PM > To: Histonet at lists.utsouthwestern.edu Histonet at lists.utsouthwestern.edu> > Subject: [Histonet] Survey!!!!!! > > Hi Everyone, > > I just wanted to get everyone's opinion. If you had to chose between > buying a Auto Side Stainer or Auto Slide Cover Slipper, which one would you > chose? Lets say your volume was around 60 to 80 blocks a day and you worked > for a GI Lab. Everyone's input would be greatly appreciated. > > > > Sincerely, > > PATTI NELSON H.T.(ASCP) > PNP LABORATORY CONSULTANTS > SUPERVISOR DGC/ZADEH LABS > PO BOX 412 > CABAZON, CA. 92230 > 909-841-9761 > nelsonrnch at verizon.net > CONFIDENTIALITY NOTICE:This message and any included attachments are from > Patti Nelson, PNP Laboratory Consultants and are intended only for the > addressee. The information contained in this message is confidential and > may contain privileged, confidential, proprietary and/or exemption from > disclosure under applicable law. Unauthorized forwarding, printing, > copying, distribution, or use of such information is strictly prohibited > and may be unlawful. If you are not the addressee, please promptly delete > this message and notify the sender ofthe delivery error by e-mail or you > may call 909-841-9761. > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > CONFIDENTIALITY NOTICE: The information contained in this electronic > message is intended solely for the personal and confidential use of the > designated recipient(s) named above and may contain information that is > protected from disclosure under applicable law. If you are not the intended > recipient, or the employee or agent responsible for delivering it to the > intended recipient, you are hereby notified that any dissemination, > distribution or copying of this transmission is strictly prohibited. If you > have received this transmission in error, please notify the transmitting > person/department immediately by email or telephone (410) 581-5881 and > delete the message without making a copy. > CONFIDENTIALITY NOTICE: The information contained in this electronic > message is intended solely for the personal and confidential use of the > designated recipient(s) named above and may contain information that is > protected from disclosure under applicable law. If you are not the intended > recipient, or the employee or agent responsible for delivering it to the > intended recipient, you are hereby notified that any dissemination, > distribution or copying of this transmission is strictly prohibited. If you > have received this transmission in error, please notify the transmitting > person/department immediately by email or telephone (410) 581-5881 and > delete the message without making a copy. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Have a great day!