[Histonet] IHC for secreted proteins and cytokines in frozen sections - Pre-fix or post-fix?

[Ana Maluenda]

Hi everyone,

Just wondering what is people's opinion and protocols for IHC in mouse frozen sections targeting secreted proteins and cytokines. I see lots of places using fresh frozen sections/snap-freeze and cold acetone or methanol/ethanol post-fixation. Is this an issue when it comes to diffusion of such proteins in the tissue, since they are not well localized or linked to cellular structures? Would it be better to pre-fix (either immersion in 4%PFA or infusion with fixative) than post-fix?

Any thoughts would be much appreciated.

Kind regards,

Ana

Ana Maluenda
Research Assistant


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