[Histonet] Fungus controls

[Dessasau III, Evan]

Hi Toysha, How long do you let the tissue/microbes incubate and at room temp?  When we tried it in the incubator at different times the tissue was too far gone to make it useful.  After a few day you still had viable tissue structure?  I'm going to try the liver prep.
Thank you so much if you are able to help!!
E-van

-----Original Message-----
From: Mayer,Toysha N via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: Tuesday, July 25, 2017 11:17 AM
To: 'histonet at lists.utsouthwestern.edu' <histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Fungus controls

We got some organisms from the microbiology lab and some fresh lung from autopsy.  When the organisms were ready, we 'stuck' the lung with the inoculating loop and let them incubate for a few days.  Then we grossed and processed the lung.  We were given separate specimens for GMs, AFB, and gram.  It worked great.
If you don't have lung, some liver from the grocery store would work.  We let that sit in the refrigerator for about two weeks, then did touch preps to see if anything was growing.
It had fungus galore in the touch prep.  We used that to teach students how to stain touch preps with specials.

Toysha

Message: 3
Date: Mon, 24 Jul 2017 09:48:16 -0400
From: Charles Riley <criley at dpspa.com>
To: Histo List <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Fungus Controls
Message-ID:
<CAAQhB10O2JT9ZTGCrAyPBFWE6Q7fcbFCQ5ABsFgmUQD_MiHOug at mail.gmail.com>
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Does anyone grow their own fungus control blocks for GMS and AFB stains?

If so would you mind sharing your procedure for doing so. I tried using rotten oranges but the sample wouldn't hold onto the counterstain.

--

Charles Riley BS  HT, HTL(ASCP)CM

Histopathology Coordinator/ Mohs


------------------------------

Message: 4
Date: Mon, 24 Jul 2017 08:11:40 -0700
From: Akemi <akemiat3377 at gmail.com>
To: Charles Riley <criley at dpspa.com>,Histonet
<Histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Fungus Controls
Message-ID: <6E924300-3BA1-4271-9B8A-8E3D9C754F89 at gmail.com>
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I've been told by one of my pathologists that blue cheese for a fungus control would work.
Akemi Allison

Sent from my iPhone

> On Jul 24, 2017, at 6:48 AM, Charles Riley via Histonet <histonet at lists.utsouthwestern.edu> wrote:
>
> Does anyone grow their own fungus control blocks for GMS and AFB stains?
>
> If so would you mind sharing your procedure for doing so. I tried
> using rotten oranges but the sample wouldn't hold onto the counterstain.
>
> --
>
> Charles Riley BS  HT, HTL(ASCP)CM
>
> Histopathology Coordinator/ Mohs
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet



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