[Histonet] IHC questions

[Gauch, Vicki]

Hi all,
I have been asked to post several questions regarding IHC stain issues and am hoping someone has some ideas...  We are having issues with IHC cases floating (particularly breast IHC) lately and are wondering....

1)      How are people storing IHC control slides?  Are they being precut and stored or cut at the time of the stain request?

2)      If storing controls- are you doing anything special ?

3)      How long are the IHC slides being air dried before placing in the oven ?  How long are they in the oven prior to staining?

This has not been such an issue in the past but recently we are experiencing it more frequently. We are looking at various things  but if anyone has any suggestions, they would be greatly appreciated !!!

Thanks,
Vicki Gauch
AMCH
Albany, NY


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