[Histonet] FW: PAX-5

[Linda Margraf]

Here is a message I am posting for Beth….


From: "O'neil, Beth" <oneilb at wvumedicine.org<mailto:oneilb at wvumedicine.org>>
Date: July 21, 2017 at 2:31:24 PM CDT
To: "histonet-owner at lists.utsouthwestern.edu<mailto:histonet-owner at lists.utsouthwestern.edu>" <histonet-owner at lists.utsouthwestern.edu<mailto:histonet-owner at lists.utsouthwestern.edu>>
Subject: PAX-5
My pathologists are not happy with PAX-5 on the Ventana Ultra; it will occasionally show cytoplasmic staining of the Reed-Sternberg cells (supposed to be a nuclear stain).  We use Ventana’s clone SP34. I have been trying to optimize with the Optiview kit but we are getting so much background staining and only sporadic staining of the R-S cells.  I tried a different clone, Cell Marque EP156 which was really clean but would not stain the R-S cells at all, no matter what we did.   My Ventana technical specialist is stumped as to why we’re having so much trouble.  Does anyone else have the same problem or have any suggestions?

Beth Ann O’Neil, MT(ASCP)SC, HTL, QIHC
Histology Supervisor, Technical Specialist
Lab:  304 – 293 – 6014
Office:  304 – 293 – 7629
 oneilb at wvumedicine.org<mailto:oneilb at wvumedicine.org>


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