[Histonet] Histonet Digest, Vol 164, Issue 10 mycoplasma on agar

Frazier, John john.frazier at roche.com
Sat Jul 15 18:21:49 CDT 2017


I would have the ordering physician to order a cold agglutinin on the
patient. This is a a good indicator if Mycoplasma is present.

Sent from my iPad

> On Jul 14, 2017, at 4:23 PM, Steve McClain <SteveM at mcclainlab.com> wrote:
>
> Too bad, Hoescht stain is rapid and sensitive (UV) for mycoplasma.
>
> They can try staining direct smears w difquik giemsa.
>
> Or fix agar plate in Carnoy's and slice out and process to paraffin for microtomy and staining.  IHC  can be done from sections if needed.
> These 1 micron bacteria are at the practical limits of visibility.
>
> Steve A. McClain, MD
>
>
>



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