[Histonet] Section adherence issues (IHC)

[Walter Benton]

Greg,

We are running into a similar issue on the Biocare Nemesis platform and the support there suggest humidity to be the issue. Our sections stay adhered to the slides through depar, antigen retrieval, but appear to fall off during the staining process. It is not consistent. We are going to try some other slides and see if the issue persists. I'd be interested to hear your findings and resolution.


Walter Benton HT(ASCP)QIHC
Lab Operations Manager
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061




-----Original Message-----
From: Greg Dobbin via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: Wednesday, July 12, 2017 11:09 AM
To: histonet at lists.utsouthwestern.edu
Cc: kgeveleigh at ihis.org
Subject: [Histonet] Section adherence issues (IHC)

Hi Folks,
I haven't been on here much lately but it is nice to know that we are all here for each other when needed!

My problem is section adherence during IHC staining. And it is not all of the time it is intermittent; so not all of the time and not on all slides when it does occur.

Background:
We have a Bond-III immunostainer and we use the Leica Apex charged slides for our IHC stains. We have no additives in our water baths. Our sections drain in a stand and then any trapped water under the section is either flicked or wicked away as needed prior to placing the slides in a rack in a
60 C oven for 30 mins prior to staining.  We do not do on board baking (primarily because on board baking is only 10 mins long and with the section lifting issue we want longer).

Some of the specimen types are more susceptible it would seem. Cervix LEEP specimens tend to be bad, we use a 4mm punch to obtain some of our control tissue and so the breast tissue we use for Myosin Heavy Chain seems to be a bad one and sometimes our ER/PR control sections (but not always). Fine needle cores where a lot of tumour is present tend to be bad (again not always).

We have tried baking longer, we turn ourselves inside out trying to get all of the water out from under the sections, we have tried charged slides from another manufacturer. I have looked at the HIER protocols and none are extraordinary in nature. We use very clean covertiles (no scratches or blemishes). Our specimens are fixed for 24hrs before processing. We use the same slides for Special Stains and don't have this issue there.

I need some new suggestions to try! All ideas welcome.
Cheers,
Greg

--
*Greg Dobbin*
1205 Pleasant Grove Rd
RR#2 York,
PE      C0A 1P0


*Everything in moderation...even moderation itself**!*
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