[Histonet] Tissues storage question

[Colleen Forster]

Greetings Blanca,

Let me think about this for a bit....I will get back to you soon!

Respectfully,

Colleen FOrster HT(ASCP)QIHC
BioNet Histology and Research Laboratory
612-626-1930

On Fri, Jan 27, 2017 at 8:41 AM, Blanca Lopez via Histonet <
histonet at lists.utsouthwestern.edu> wrote:

> Dear Histonets,
> I would like to ask everybody what do you think about a specific way to
> storage tissues in -80 C for long time of periods and how this type of
> procedure can affect those tissues.
>                This is my first time that I heard about this procedure:
> I have a customer that storage hundreds of tissues this way, she fixed
> them in 4 paraformaldehyde for long time then transfer to a 100% alcohol
> and storage them in -80 C for several years...Can you told me if this
> procedure is wrong or good and how can affect those tissues?
>                I told her that I can test a couple to see how they
> react...I process them starting 70% alcohol for 12 hrs. processing, cut
> them (they were little crunchy) and perform an H&E. everything stain pink
> except the nucleus were fade purple. It looks weird to me.
>
> My question is how can she troubleshooting those tissues? Can we fix them
> in formalin again and start a new process? Can we wash the tissues in soap
> water to clean them? How can we do to make those tissue back to normal? Is
> alcohol a bad storage reagent?
>
> I am kind of loss with this case. I really need to understand how the
> tissue get affected to be storage this way. How can I troubleshooting?
>
> Thanks for your advices:)
>
> Blanca Lopez
> Histotech (ASCP)
> UTSW Tissue Resource K1.210
> Simmons Comprehensive Cancer Center
> UT Southwestern Medical Center
> Telephone: 214-648-7598
> Email: Blanca.Lopez at utsouthwestern.edu
>
>
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