[Histonet] xenopus oocyte fixation

[Tamara A Howard]

Monica -


The Klymkowsky lab has a trove of Xenopus protocols:


http://klymkowskylab.colorado.edu/


Their wax and cryo page also has the reference to the 1989 Methods in Cell Biology paper covering Xenopus histological methods.


If you can, leave the oocytes in the ovary - it is MUCH easier to handle a blob of them for embedding than single oocytes!


Tamara


Tamara Howard
Dept. of Cell Biology & Physiology
UNM-HSC
________________________________
From: Monica Aguilera <monica.aguilera at irbbarcelona.org>
Sent: Thursday, January 19, 2017 9:52 AM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] xenopus oocyte fixation

Hello all,

does anyone have a good protocol (if possible) for xenopuss oocyte
fixation+paraffin embedding or cryopreservation+OCT embedding?


Many thanks in advance!

Kind Regards,
Mònica

--
Mònica Aguilera Pujabet, DVM, PhD
Histopathology Facility
Institute for Research in Biomedicine - IRB Barcelona
Baldiri Reixac, 10
E-08028 Barcelona - Spain

Tel:  +34 934033776 <%2B34%20934020546>
monica.aguilera at irbbarcelona.org <neus.prats at irbbarcelona.org>