[Histonet] Histonet Digest, Vol 159, Issue 16 Colloidal iron leaching

[Steve McClain]

After checking reagents and glassware, look for other sources of iron contamination, e.g. Metal forceps, steel staining racks, water bath. 

Inadequate rinsing after the colloidal iron step - increase wash times, using DI

Check concentration of working reagent. Decrease the time in colloidal iron working reagent to 5 min. Hales method specified 5-20 min.

> Steve A. McClain, MD
> 
>>> Hello netters,
>>> We have had a problem with our colloidal iron leaching for about a year now. We have changed all reagents and tried different kits. Has anyone else had this problem? I cannot figure out what it is it's making me crazy! We go directly into clean 100% alcohol then clearing. Any advice would be greatly appreciated, thanks in advance!
>>> 
>>> Sent from my iPhone