[Histonet] Prostate needle biopsies

Porter, Douglas Douglas.Porter at sparrow.org
Fri Dec 15 18:39:20 CST 2017

We have stopped cutting unstained slides as physicians have started asking for the block to be sent for molecular studies.
When we cut unstained slides, there isn't enough of the specimen left for those studies.

Douglas A. Porter, HT (ASCP)
Pathologist Assistant
Anatomic Pathology IT Coordinator

Sparrow Laboratories
Department of Pathology
2508 South Cedar Street
Lansing, MI 48910-3138

517-372-5520 (phone)
517-372-5540 (fax)

douglas.porter at sparrow.org

-----Original Message-----
From: Bob Richmond via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: Friday, December 15, 2017 7:18 PM
To: Histonet at lists.utsouthwestern.edu
Subject: Re: [Histonet] Prostate needle biopsies

Martha Ward at Wake Forest Baptist Health, Winston-Salem, North Carolina

>>I am posting this question for our Histology manager. For prostate
biopsies how many levels and unstained slides are people cutting and also how long after the case is signed out is everyone keeping the unstained slides that have been cut?<<

A reasonable protocol is 5 sections from each block, cut subserially onto slides suitable for IHC. Stain slides 1, 3, and 5 with H&E for immediate review by the pathologist. Roughly 10% of cases will require immunohistochemistry on slides 2 and 4.

Many histotechnologists refuse to do this, but recutting loses about half the features of interest, compared to IHC done on pre-cut slides 2 and 4.

I don't know about retaining slides 2 and 4 in the 90% of cases where no IHC is necessary. Prolonged retention isn't necessary, though.

Robert S. Richmond
Samurai Pathologist
Maryville TN
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