[Histonet] Problem with breast cancer TMA - core are mooving

[Logan, Shannon]

Hello Liliane,

I would suggest longer time in 60 degree oven before H&E staining… perhaps 60 min.
Also, if you are not using “plus” or coated slides , I would try using them.
Good luck,

Shannon H. Logan  B.S., HTL (ASCP)
Pathology Department

Bellin Health Memorial Hospital
744 South Webster Avenue
Green Bay, WI 54305-3400
920-433-3653  X3727





From: Liliane Meunier via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: Tuesday, December 12, 2017 9:33 AM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Problem with breast cancer TMA - core are mooving

Hi

I recently did a H&E coloration on a TMA that was cut the day before. TMA
contain 300 breast tumor core. After the coloration, I noticed that a lot
of core had moove. I put slide 20 minutes in an oven at 60oC prior to
coloration

Thanks a lot for your suggestions


Liliane Meunier


Assistante de recherche
Laboratoire Dre Anne-Marie Mes-Masson
Institut du Cancer de Montréal
Plateforme Pathologie Moléculaire CRCHUM
900, rue St-Denis
10e étage
Montréal, Qc H2X 0A9
514-890-8000 poste 31296
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