[Histonet] Paraformaldehyde Fixed Tissue

[Tina Van Meter]

Hi Sandra,
What are the times for each processing station?​ Animal tissue is much
leaner, so it's easy to over process and become brittle to section.

Best,
Tina Van Meter




On Mon, Aug 28, 2017 at 11:30 AM, Sandra Cheasty via Histonet <
histonet at lists.utsouthwestern.edu> wrote:

> Hi all,
>                 We are having difficulty sectioning mouse tissue, (brain,
> spinal cord, liver, and spleen), on paraformaldehyde fixed tissue. Has
> anyone had issues with paraformaldehyde fixed tissue? They were processed
> routinely, starting in 10% NBF, with other tissues, and we are cutting them
> at 3u.
> Thank you!
> Sandy
>
> Sandra J. Cheasty, HT (ASCP)
> Histology & Necropsy Supervisor
> UW-Madison, School of Veterinary Medicine
>
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