[Histonet] Fwd: Dissolving precipitate in formalin?

[Conway, Carla]

Hi Lauren,

Thanks so much for your reply. I've seen the precipitate you refer to, but
this looks really different. Many of the flakes are large (5-7 mm) and were
at the bottom of the bottle. The bottles were previously unopened and
I didn't notice the flakes until preparing fixative for a study this
morning. Am wondering if adding NaOH will help (similar to dissolving
paraformaldehyde for EM fixative).

Thanks again!

Carla


Carla Conway
Histology Technician
Western Fisheries Research Center, USGS
6505 N.E. 65th Street
Seattle, WA 98115-5016 USA
Phone: 206-526-2042
Fax: 206-526-6654
E-mail: cmconway at usgs.gov

---------- Forwarded message ----------
From: Lauren Sweeney <lmarie08 at uga.edu>
Date: Tue, Aug 22, 2017 at 8:02 AM
Subject: RE: [Histonet] Dissolving precipitate in formalin?
To: "Conway, Carla" <cmconway at usgs.gov>


I've always been told that that is the salts flaking out and that you can't
dissolve them, usually found accumulating around the spigot or opening of
wherever it is poured out, we always just brush it off and if re-using the
container, can dissolve after running under very hot tap water. Hope this
helps!

-----Original Message-----
From: Conway, Carla via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: Tuesday, August 22, 2017 10:39 AM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Dissolving precipitate in formalin?

Hello,

I have 4 bottles of formalin which contain white flakes of paraformaldehyde
precipitate. I tried to dissolve the flakes by heating to ~65 degrees C for
45 min (with stirring). Has anyone been successful in dissolving this type
of precipitate?

Thanks very much,

Carla

Carla Conway
Histology Technician
Western Fisheries Research Center, USGS
6505 N.E. 65th Street
Seattle, WA 98115-5016 USA
Phone: 206-526-2042
Fax: 206-526-6654
E-mail: cmconway at usgs.gov
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