[Histonet] "Dirty" Immunos

Elizabeth Chlipala liz at premierlab.com
Thu Aug 10 10:57:10 CDT 2017


Paula

You need to sit down with them and review the slides together, you can't solve a problem without seeing the problem.  They need to explain and show you what they consider dirty.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
liz at premierlab.com
www.premierlab.com

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Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504

-----Original Message-----
From: Paula via Histonet [mailto:histonet at lists.utsouthwestern.edu] 
Sent: Thursday, August 10, 2017 8:24 AM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] "Dirty" Immunos

Hello,

Hoping we could get a recommendation on helping this issue..

We have a pathologist that complains about "dirty" stains every now and then..not all the time. What could this be/mean? Could it be debris, background staining?

We use the Bond III with their ready to use antibodies and their slides for tissue controls.  

 

Regarding the "dirty" complaint, could it come from our water baths, covertiles, paraffin, antibodies? 

 

I appreciate any feedback to help and I'll try anything.

Thank you,

Paula

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