[Histonet] PIN4 not working

[Eddie Martin]

Hi Kim,

I was wondering if you could be more descriptive as to what is going wrong with your PIN4 stain?

It's difficult to give a suggestion when we don't have any clue as to what your lab is visualizing. 

Is it the DAB?
Is it the AP Fast Red chromagen?
Is it reduced staining or no staining?

Kind regards,

Eddie Martin, HTL, HT(ASCP), QIHC(ASCP)
IHC Histotechnologist III
Joint Pathology Center (JPC)
Walter Reed National Military Medical Center
Bethesda, MD
 



> On Aug 4, 2017, at 5:06 PM, Renee Fisher <rfisher0126 at msn.com> wrote:
> 
> Need help the PIN4 has suddenly stop working we use P63+CK5+CK14 from Bio Care Medical and P504S from Dako.  We double stain on the Leica Bond with the Polymer refine Red and Polymer Refine detection. This stain has worked beautifully in the past. Any suggestions?
> 
> 
> 
> Thank you
> 
>