[Histonet] Breast Her2Neu IHC vs FISH

[Morken, Timothy]

Jason, 

The only way is to validate for those conditions. You would need some specimens, including known controls,  handled and processed in those same conditions and compare to samples processed under the acceptable conditions. You can do it, it will just take some time. 


Tim Morken
Pathology Site Manager, Parnassus 
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center



-----Original Message-----
From: Jason McGough via Histonet [mailto:histonet at lists.utsouthwestern.edu] 
Sent: Wednesday, April 19, 2017 12:29 PM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Breast Her2Neu IHC vs FISH

Does anyone know or can you point me in the right direction to some literature about how to properly test for Her2Neu (IHC vs. FISH) on breast tumors if the cold ischemia time is greater than 1 hour or if the formalin fixation times are outside of the recommended time range? Also, what if the specimen has been placed in decal? We are struggling to find any documentation of how to properly test Her2Neu if the specimen is outside of these ranges. Thanks for your help!



Jason McGough, HT(ASCP)

Operations Manager

Clinical Laboratory of the Black Hills

605-343-2267

jmcgough at clinlab.com <mailto:jmcgough at clinlab.com> 

www.clinlab.com <http://www.clinlab.com> 

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