[Histonet] processor died overnight

Wed Apr 19 14:31:21 CDT 2017

To help this you could place the cassettes in the other processor in the last 100% for about 5 min, just to freshen them up.  Then proceed with the remainder of the process as usual.  

FYI, this will be one of our discussion questions this year in our HTL program.

T
Toysha N. Mayer D.H.Sc., MBA, HT(ASCP)
Instructor/Education Coordinator
HTL Program
MD Anderson School of Health Professions
713.563.3481
tnmayer at mdanderson.org

Hello Histoworld,

I came in this morning to find that the processor died halfway through process last night. The tissues are in 100% ETOH exactly half point. We do have a back- up processor. In your professional experiences, would these tissues be salvageable? Could I create a new program on the backup processor that finishes the process from that point and transfer the tissues over?

Thanks.

------------------------------

Message: 5
Date: Wed, 19 Apr 2017 13:41:47 +0000 (UTC)
From: Rene J Buesa <rjbuesa at yahoo.com>
To: Lauren Sweeney <lmarie08 at uga.edu>,
	"histonet at lists.utsouthwestern.edu"
	<histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] processor died overnight
Message-ID: <174147213.3806623.1492609307572 at mail.yahoo.com>
Content-Type: text/plain; charset=UTF-8

In 100% EthOL the tissues are completely "salvaged" and you can prepare the program to continue the steps until melted paraffin.If there are delicate tissue perhaps they will be "over-dried" but that is easily "compensated" during microtomy.Ren? 

    On Wednesday, April 19, 2017 9:01 AM, Lauren Sweeney via Histonet <histonet at lists.utsouthwestern.edu> wrote:

 Hello Histoworld,

I came in this morning to find that the processor died halfway through process last night. The tissues are in 100% ETOH exactly half point. We do have a back- up processor. In your professional experiences, would these tissues be salvageable? Could I create a new program on the backup processor that finishes the process from that point and transfer the tissues over?

Thanks.

_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 6
Date: Wed, 19 Apr 2017 16:11:51 +0000
From: "Terri  Braud" <tbraud at holyredeemer.com>
To: "'histonet at lists.utsouthwestern.edu'"
	<histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] IF vs IHC another reason
Message-ID:
	<48E053DDF6CE074DB6A7414BA05403F8130EAF at HRHEX03-HOS.holyredeemer.local>

Content-Type: text/plain; charset="us-ascii"

Another reason that IHC is used instead of IF is with IHC, one preserves the ability to see tissue/cell morphology through Light Microscopy at the same time as the visual IHC label.  Morphology is difficult to see with IF, with the exception of the fluorescein labeled area.

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
Care, Comfort, and Heal
-----Original Message-----
From: Blanca Lopez via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: Thursday, 13 April 2017 11:10 PM
Hello!
I just need a help with a simple question...Is anyone can explain me what is the purpose between performing immunohistochemistry and Immunofluorescence?
Thanks  :)
Blanca Lopez
Histotech (ASCP)
UTSW Tissue Resource K1.210
Simmons Comprehensive Cancer Center
UT Southwestern Medical Center
Telephone: 214-648-7598
Email: Blanca.Lopez at utsouthwestern.edu

------------------------------

Message: 7
Date: Wed, 19 Apr 2017 09:17:24 -0700
From: Caroline Miller <mills at 3scan.com>
To: Rene J Buesa <rjbuesa at yahoo.com>
Cc: Lauren Sweeney <lmarie08 at uga.edu>,
	"histonet at lists.utsouthwestern.edu"
	<histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] processor died overnight
Message-ID: <6156911D-EDE8-4C77-9C6D-36BA64BAF03F at 3scan.com>
Content-Type: text/plain;	charset=utf-8

Yes, totally +1 to Rene, they should be fine. 
(That has totally happened to me too)!

Caroline Miller (mills)
Director of Histology
3Scan, Inc
415-2187297

> On Apr 19, 2017, at 6:41 AM, Rene J Buesa via Histonet <histonet at lists.utsouthwestern.edu> wrote:
> 
> In 100% EthOL the tissues are completely "salvaged" and you can prepare the program to continue the steps until melted paraffin.If there are delicate tissue perhaps they will be "over-dried" but that is easily "compensated" during microtomy.Ren? 
> 
>    On Wednesday, April 19, 2017 9:01 AM, Lauren Sweeney via Histonet <histonet at lists.utsouthwestern.edu> wrote:
> 
> 
> Hello Histoworld,
> 
> I came in this morning to find that the processor died halfway through process last night. The tissues are in 100% ETOH exactly half point. We do have a back- up processor. In your professional experiences, would these tissues be salvageable? Could I create a new program on the backup processor that finishes the process from that point and transfer the tissues over?
> 
> Thanks.

Message: 8
Date: Wed, 19 Apr 2017 16:26:56 +0000
From: "McNabola, Angela" <Angela.McNabola at bpthosp.org>
To: 'Caroline Miller' <mills at 3scan.com>,
	"histonet at lists.utsouthwestern.edu"
	<histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] processor died overnight
Message-ID: <69AA9D996B56BF4EBCBB8EA666461B9326EBBB32 at mbx9vp.YNHH.ORG>
Content-Type: text/plain; charset="utf-8"

It has happened to us as well.  I'm not sure where you work, but our processors have alarms that go to security.  So I have gotten a call on off hours to come in and fix the problem or try to trouble shoot.  Some of the newer processors can be linked to your phone, etc.

-----Original Message-----
From: Caroline Miller via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: Wednesday, April 19, 2017 12:17 PM
To: Rene J Buesa
Cc: histonet at lists.utsouthwestern.edu
Subject: Re: [Histonet] processor died overnight

Yes, totally +1 to Rene, they should be fine.
(That has totally happened to me too)!

Caroline Miller (mills)
Director of Histology
3Scan, Inc
415-2187297

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