[Histonet] help!!

[Allan Wang]

Tim and Tony,

Why couldn't DAB be used on frozen sections in your example?

Allan

On Mon, Apr 17, 2017 at 9:03 PM, Tony Henwood (SCHN) via Histonet <
histonet at lists.utsouthwestern.edu> wrote:

> Hi Bianca,
> Well for most Pathology departments, Immunofluorescence (IF) is used for
> Renal and Skin biopsies; looking for human Immunoglobulin (Igs) deposition
> on basement membranes. The advantage here is not so much the fluorescence,
> but that we use unfixed frozen sections. The buffer rinse before antibody
> application, removes un-bound serum immunoglobulins, leaving any
> pathological bound Igs for the IF antibody to bind to. This gives a clean
> result.
>
> If one would do IF on formalin-fixed paraffin sections of renal or skin
> biopsies, you would find heavy background due to the fixative cross-linking
> serum Igs to tissue and cells (which would usually be removed by the buffer
> rinse if unfixed frozen sections were used - see above).
>
> IF, apart from being a historic method, also does not suffer from
> endogenous peroxidase that would need to be blocked if peroxidase was used
> in place of fluorescence.
>
>
>
> Regards
> Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
> Principal Scientist, the Children's Hospital at Westmead
> Adjunct Fellow, School of Medicine, University of Western Sydney
> Tel: 612 9845 3306
> Fax: 612 9845 3318
> Pathology Department
> the children's hospital at westmead
> Cnr Hawkesbury Road and Hainsworth Street, Westmead
> Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
>
> -----Original Message-----
> From: Blanca Lopez via Histonet [mailto:histonet at lists.utsouthwestern.edu]
> Sent: Thursday, 13 April 2017 11:10 PM
> To: histonet at lists.utsouthwestern.edu
> Subject: [Histonet] help!!
>
> Hello!
> I just need a help with a simple question...Is anyone can explain me what
> is the purpose between performing immunohistochemistry and
> Immunofluorescence?
> Thanks  :)
>
> Blanca Lopez
> Histotech (ASCP)
> UTSW Tissue Resource K1.210
> Simmons Comprehensive Cancer Center
> UT Southwestern Medical Center
> Telephone: 214-648-7598
> Email: Blanca.Lopez at utsouthwestern.edu
>
>
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