[Histonet] Hirsch-Pfeiffer cresyl violet method

[Morken, Timothy]

Bob, Thanks for the offer! I finally found a copy and Google Translate did a pretty good job on it, and we have a person in the lab who, it turns out, can read german, so I think we are set.

As an aside, the "clues" I got from tidbits of the procedure mentioned in other papers turned out to have little basis in reality to the original method described in the paper (concentrations, times, temperatures all different!), so I'm glad I found the original.


Tim Morken
Pathology Site Manager, Parnassus 
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center



-----Original Message-----
From: Bob Richmond via Histonet [mailto:histonet at lists.utsouthwestern.edu] 
Sent: Saturday, April 15, 2017 11:46 AM
To: Histonet at lists.utsouthwestern.edu
Subject: Re: [Histonet] Hirsch-Pfeiffer cresyl violet method

Tim Morken in pathology at UC San Francisco Medical Center asks about the Hirsch-Pfeiffer (correct spelling) cresyl violet stain for frozen sections.

"I've found many references to it, but none that give the procedure. And the original paper is from 1955 not easily available. And is in German."

If you can get the paper, I can read it for you.

Bob Richmond
Samurai Pathologist
Maryville TN
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