[Histonet] Anti-fibrin antibody

[Ana Maluenda]

Dear all,

I've been trying to optimise an immunohistochemistry protocol for fibrin that has not been working. Was wondering if there is anyone that would have any recommendation of staining protocols for fibrin or recommendation of anti-fibrin antibodies?
We are working with cryosections, mouse tissue, embedded fresh (no pre-fixation). I've tried different conditions already (different fixatives, blocking reagents and so on). So far, the antibodies I found commercially available are anti-mouse fibrinogen. This IHC is to complement special stainings (eg Masson and PSR).

Thanks in advance.

Kind regards,

Ana


Ana Maluenda
Research Assistant
Atherothrombosis and Vascular Biology Laboratory

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