[Histonet] Mousse tissue processing

Caroline Miller mills at 3scan.com
Wed Sep 21 17:37:03 CDT 2016


Hi Blanca, This is what I use, note, no formalin on my machine, and you
could probably cut out one of the last alcohols

Step Routine tissue
70% alcohol 10
80% alcohol 10
90% alcohol 20
100% alcohol 20
100% alcohol 20
100% alcohol 30
100% alcohol 30
100% alcohol 30
Xylene 30
Xylene 35
paraffin 30
paraffin 30
paraffin 30
paraffin 30
paraffin at 60oC, P/V on, agit on

On Wed, Sep 21, 2016 at 12:59 PM, Blanca Lopez via Histonet <
histonet at lists.utsouthwestern.edu> wrote:

> Hello! Histonets.
> Does anyone has a good protocol for processing mouse tissue? Somebody can
> share.
> thanks
>
> Blanca Lopez
> Histotech (ASCP)
> UTSW Tissue Resource K1.210
> Simmons Comprehensive Cancer Center
> UT Southwestern Medical Center
> Telephone: 214-648-7598
> Email: Blanca.Lopez at utsouthwestern.edu
>
>
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-- 
Caroline Miller (mills)
Director of Histology
3Scan.com
415 2187297


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