[Histonet] head kidney issues
carl.hobbs at kcl.ac.uk
Thu Sep 15 01:49:47 CDT 2016
It's endogenous biotin, Tyrone.
Make your own biotin blocking kit or buy RTU kit.
Also, endog. peroxidases are eliminated by 3% aq H2O2 ( saves methanol) for 10mins at RT
Also, don't bother with permeabilisation.....you've done all that by processing the tissue to Pwax
Also, use 10% Formalin.
That's what I use for my zfish Pwax IHC
All suggestions: imho.
Have a look in Histonet image archives for IHC Pwax sections of z.fish
Carl Hobbs FIBMS
Histology and Imaging Manager
Guys Campus, London Bridge
Kings College London
020 7848 6813
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