[Histonet] Egg Whites and IHC Staining

[Elizabeth Chlipala]

Jessica

There are a couple of things that we will try here when we have problems with tissue adherence

1.  We utilize a particular brand of plus slides  - trubond 380 - these have worked very well in our hands, we purchase them from Newcomer Supply
2.  Post fix in 10% NBF for 10 minutes after you run the slides down to water - this does not impact the H&E staining but can impact IHC staining we have seen that is may decrease sensitivity so you would need to potentially rework your IHC protocol
3.  Our Ram-11 IHC protocol requires HIER we have two different retrieval protocols that we have validated for this particular antibody - our routine 95C for 30 minutes and then a 70C for 2 hours - we use the longer lower temp retrieval when we have problems with tissue adherence, again you may need to potentially rework your IHC protocol for the lower temp retrieval.

Hope this helps, good luck

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
liz at premierlab.com
www.premierlab.com

Ship to Address:

Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504

-----Original Message-----
From: Jessica Riggleman via Histonet [mailto:histonet at lists.utsouthwestern.edu] 
Sent: Monday, September 12, 2016 8:05 AM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Egg Whites and IHC Staining

Hello,

We are cutting large Rabbit PLF formalin fixed paraffin embedded sections.

We have had issues staining H&E and Goldner’s Trichrome. Egg whites is working as a great slide adhesive for these two, but not so much for IHC Staining (RAM 11 specifically).

Any ideas/suggestions on how to get these sections to adhere? We have tried many methods, such as positively charged slides, chrom-alum gel, and so on.

Thank you for all input,
Jessica


_____________________________________________________________________

Jessica Riggleman | Research Associate

Globus Medical, Inc.
Valley Forge Business Center
2560 General Armistead Avenue | Audubon, PA 19403
Ph: (610) 930-1800 ext. 2583 | Fax:

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