[Histonet] Egg Whites and IHC Staining

Rene J Buesa rjbuesa at yahoo.com
Mon Sep 12 09:59:09 CDT 2016

Egg white (in Mueller's albumin) will always produce a "shadowy" staining with IH procedures y a dark background with IHC procedures.I suggest you use pork gelatin dissolves in the water of the water bath, use (+) charged slides and increase the drying time in the oven.Also sectioning those large pieces as thin as possible will help.René 

    On Monday, September 12, 2016 10:25 AM, Jessica Riggleman via Histonet <histonet at lists.utsouthwestern.edu> wrote:


We are cutting large Rabbit PLF formalin fixed paraffin embedded sections.

We have had issues staining H&E and Goldner’s Trichrome. Egg whites is working as a great slide adhesive for these two, but not so much for IHC Staining (RAM 11 specifically).

Any ideas/suggestions on how to get these sections to adhere? We have tried many methods, such as positively charged slides, chrom-alum gel, and so on.

Thank you for all input,


Jessica Riggleman | Research Associate

Globus Medical, Inc.
Valley Forge Business Center
2560 General Armistead Avenue | Audubon, PA 19403
Ph: (610) 930-1800 ext. 2583 | Fax:

Confidentiality Note:  This email is confidential and intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing, or copying of this email is strictly prohibited. If you have received this email in error please contact the sender. Any views or opinions presented are solely those of the author and do not necessarily represent those of Globus Medical, Inc. Although this email and any attachments are believed to be free of any virus or other defects which might affect any computer or IT system into which they are received, no responsibility is accepted by Globus Medical, Inc. for any loss or damage arising in any way from the receipt or use thereof.

Histonet mailing list
Histonet at lists.utsouthwestern.edu


More information about the Histonet mailing list