[Histonet] Egg Whites and IHC Staining

[Rene J Buesa]

Egg white (in Mueller's albumin) will always produce a "shadowy" staining with IH procedures y a dark background with IHC procedures.I suggest you use pork gelatin dissolves in the water of the water bath, use (+) charged slides and increase the drying time in the oven.Also sectioning those large pieces as thin as possible will help.René 

    On Monday, September 12, 2016 10:25 AM, Jessica Riggleman via Histonet <histonet at lists.utsouthwestern.edu> wrote:
 

 Hello,

We are cutting large Rabbit PLF formalin fixed paraffin embedded sections.

We have had issues staining H&E and Goldner’s Trichrome. Egg whites is working as a great slide adhesive for these two, but not so much for IHC Staining (RAM 11 specifically).

Any ideas/suggestions on how to get these sections to adhere? We have tried many methods, such as positively charged slides, chrom-alum gel, and so on.

Thank you for all input,
Jessica


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