[Histonet] H. pylori stain

Duddey, Aimee ADuddey at firsthealth.org
Fri Sep 2 07:23:01 CDT 2016

We have BMK Ultras for years.  I would suggest running the decontam for the stainer with the prescribed Lysol IC solution and then see what you get.  It the trash in your control tissue too or just the patient tissue?


-----Original Message-----
From: Amy Johnson via Histonet [mailto:histonet at lists.utsouthwestern.edu] 
Sent: Thursday, September 01, 2016 3:25 PM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] H. pylori stain

Hello histoland

We currently use Ventana's h. pylori antibody on our Benchmark ultra.  Over the last few months our pathologists have been noticing "junk" that is staining with this antibody.  It seems to be on the tissue more than in the tissue and its not on all slides.  WE have tried changing our waterbath from tap water to distilled water with no difference in staining.  We thought maybe our paraffin was bad so we stained a "blank" block of paraffin but could not find the culprit.......Any insight would be appreciated!

Amylin Johnson, B.S. HTL(ASCP)
Associates in Pathology
Wausau Wi 54401

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