[Histonet] Villanueva Osteochrome Bone Stain on already embedded ground sections

Alicia Marie Ortega alicia.ortega at colorado.edu
Tue Oct 11 18:47:01 CDT 2016

Hello everyone,

I was just wondering if anyone has a protocol for staining undecalcified bone already embedded in poly(methyl methacylate) using the Villanueva Osteochrome Bone Stain?  I have 30-40 micron thick samples and I cannot deplasticize my samples as suggested by the manufacturer's protocol because my samples have been glued-to and ground-on plastic slides.

Thanks in advance for your help!


Alicia Ortega

Postdoctoral Research Associate

University of Colorado, Boulder

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