[Histonet] Performing PAS with Diastase by Hand

[Moe, Barbi A]

Jim -
We use amylase from porcine pancreas, from Sigma. 

Barb Moe
Gundersen Health System
La Crosse WI 

________________________________________
From: Ingles Claire via Histonet [histonet at lists.utsouthwestern.edu]
Sent: Wednesday, October 05, 2016 5:06 PM
To: Vickroy, James; histonet at lists.utsouthwestern.edu
Subject: Re: [Histonet] Performing PAS with Diastase by Hand

We get our Diastase powder from American Mastertech.

Claire
Dermatopathology lab
University of Wisconsin Hospital and Clinics

________________________________________
From: Vickroy, James via Histonet [histonet at lists.utsouthwestern.edu]
Sent: Wednesday, October 05, 2016 3:48 PM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Performing PAS with Diastase by Hand

We are starting to perform a PAS with diastase for fungus to be used by our dermatopathologist.   At first we weren't sure he was going to need a method that used diastase but he now prefers it.  Problem is our PAS counter kit did not come with diastase.  Please don't tell me to use saliva although I'm old enough to know we did for years.  We did order some powered diastase that was quite expensive and of course the working solution had a very short shelf-life.   Can anybody recommend a product and vendor that is relatively inexpensive and economical to use?

Jim

Jim Vickroy
Histology Manager
Springfield Clinic, Main Campus, East Building
1025 South 6th Street
Springfield, Illinois  62703
Office:  217-528-7541, Ext. 15121
Email:  jvickroy at SpringfieldClinic.com<mailto:jvickroy at SpringfieldClinic.com>



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