[Histonet] Looking for a paper

[Jorge A. Santiago-Blay]

Looking for a paper

Dear Colleagues:

Two major library searches by two different institutions and years of
looking by a very competent librarian, still unable to find the reference
below:

Cunha, A. and W. E. Kerr. 1957. A genetical theory to explain
sex-determination by arrhenotocous parthenogenesis.Forma et functio
1(4):___-___.

Does anyone have this reference? More broadly, does this reference exist?

If anyone has a constructive suggestion, please email it to me directly,
blayjorge at gmail.com

Gratefully,

Jorge

P.S. Apologies for potentially duplicate emails.

Jorge A. Santiago-Blay, PhD
http://paleobiology.si.edu/staff/individuals/santiagoblay.cfm

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On Mon, Oct 3, 2016 at 3:33 PM, Jayasri Narasimhan via Histonet <
histonet at lists.utsouthwestern.edu> wrote:

> Hi everyone,
>
> For the past two months in the research lab I work in, we've been dealing
> with poorly processed mouse livers. The following is the protocol, which is
> optimized for mouse mammary tissue, but we've been using it for all tissues.
>
> Fixation:
> ~48 hours in 10% nbf, shaking.
> 70% ethanol until processing; it sits in there anywhere from a couple of
> days to weeks.
>
> Processing (Sakura Tissue Tek VIP - 5)
> 70% ethanol       5 min
> 80%                        30 min
> 95% - 1                  30 min
> 95% - 2                  40 min
> 100% -1                30 min
> 100% -2                30 min
> 100%-2                 30 min
> Xylene-1              45 min
> Xylene-2              40 min
> Xylene-3              30 min
> Paraffin1              40 min
> Paraffin2              45 min
> Paraffin3              45 min
>
> The worst livers expanded on the ice bath, and had the hepatocytes
> appeared to have perinuclear cytoplasmic clearing. We ran PAS and glycogen
> doesn't fully account for their appearance.
>
> We've pm'ed our processor, compared runs on our processor to runs from the
> research histocore, and processed a few cassettes in surgical pathology
> using their protocol.
>
> By far, livers processed in Surg Path were the best. Their protocol starts
> with NBF and then goes into 80% alchohol. From then, each cycle is 40-60
> minutes, so a bit longer than ours.
>
> Finally, the Sakura tech ran a warm water flush. The specimens run after
> this flush look similar to the ones run at the research histocore, so the
> processing problems seem to be resolved.
>
> My main question: what could the warm flush have done to resolve our
> processing issues? Since we're storing our tissues in 70% before putting
> them on the processor, aren't we preventing salt precipitation that could
> affect processing? Does anyone know the chemistry/science behind what might
> have happened here?
>
> Thanks,
> JN
> OHSU Lab
>
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



-- 
Jorge A. Santiago-Blay, PhD
blaypublishers.com

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2. Free examples of papers published in *LEB*:
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http://blayjorge.wordpress.com/
http://paleobiology.si.edu/staff/individuals/santiagoblay.cfm