[Histonet] Uneven ER/PR

[Terri Braud]

My first 2 items to check whenever I have uneven IHC staining are (1) Inadequate deparaffinization, (2) bad lot of charged slides - yes this can cause terribly streaky or spotty staining, and since you are using the Bond, perhaps there was an issue with coverplate placements.
Just things to consider.  Hope this helps, Terri

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874

Today's Topics:
   2. ER/PR Uneven Staining (Joanne Clark)
Message: 2
Date: Tue, 29 Nov 2016 21:09:17 +0000
From: Joanne Clark <jclark at pcnm.com>
Subject: [Histonet] ER/PR Uneven Staining
I had a breast needle core today that when stained with ER and PR the staining was uneven throughout the core, even though the cancer cells were present in the entire core.  The specimen had 10 hours fixation in 10% NBF.  I could understand the uneven staining from inadequate fixation on large grossed in breast tissue, but 10 hours with  needle core biopsies has always been more than sufficient.  Does anyone have any ideas?  We use Leica's ER and PR antibodies on the BOND.
Joanne Clark, BAAS, HT(ASCP)CM
Director of Histology