[Histonet] Weigert's hematoxylin for IHC

[Rene J Buesa]

Once you finish the IHC procedure, the DAB reaction is very stable and you can use Weigert's or any other iron hematoxylin.René 

    On Tuesday, November 22, 2016 5:29 PM, Esther C Peters via Histonet <histonet at lists.utsouthwestern.edu> wrote:
 

 Could someone advise me on whether Weigert's iron hematoxylin can be used as the counterstain for nuclei in IHC? We need to avoid using Harris's hematoxylin because that will stain mucocytes in the coral tissues we are working with. If it can be used, would that be after all antibody steps have been done? Thank you!


Esther Peters


Esther C. Peters, Ph.D.
Term Associate Professor
Environmental Science & Policy
George Mason University
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