[Histonet] Weigert's hematoxylin for IHC

Esther C Peters epeters2 at gmu.edu
Tue Nov 22 16:24:11 CST 2016

Could someone advise me on whether Weigert's iron hematoxylin can be used as the counterstain for nuclei in IHC? We need to avoid using Harris's hematoxylin because that will stain mucocytes in the coral tissues we are working with. If it can be used, would that be after all antibody steps have been done? Thank you!

Esther Peters

Esther C. Peters, Ph.D.
Term Associate Professor
Environmental Science & Policy
George Mason University
4400 University Drive, MS 5F2
Fairfax, VA 22030-4444
Office: David King Hall, Room 3050
Phone: 703-993-3462
Fax: 703-993-1066
e-mail: epeters2 at gmu.edu<mailto:epeters2 at gmu.edu>

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