[Histonet] A suitable negative control for BAP1

Hugh Luk Hlukey at msn.com
Thu Nov 10 16:00:37 CST 2016


We've been studying BAP-1 too and do not have a good answer for a truly negative tissue, except for skeletal muscle, but decided against it.  Our current multi control block contains:  1) lung ca, 2) tonsil, 3) colon ca, 4) LN, 5) ovary ca, 6) lung.  Our clone specifically stains each tissue, either nuclear or cytoplasmic.  My pathologists agonized over this for awhile, before they were happy with this mix.



Date: Tue, 8 Nov 2016 16:10:37 -0500
From: Patrick Laurie <foreightl at gmail.com>
Subject: [Histonet] A suitable negative control for BAP1

Hello everyone,

We recently brought BAP1 into our IHC menu, and we were eventually able to
get a good stain that our pathologist was happy with.  We are, however,
looking for a good suitable negative control.  Our pathologist is not
certain and wanted me to ask the local community.

Patrick Laurie(HT)ASCP QIHC
Histology Manager
Celligent Diagnostics, LLC
101 East W.T. Harris Blvd  | Suite 1212 | Charlotte, NC 28262
Work: 704-970-3300  Cell: 704-266-0869

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