[Histonet] Pap stain without xylene

[Mike Toole]

René and other Histonetters,


After reading the paper:



Buesa RJ, Peshkov, MV. Histology without xylene. Ann Diagn Pathol. 2009 Aug;13(4):246-56. Epub 2009 Feb 5.



It appears that xylene in the final clearing steps is replaced with isopropanol and mineral oil as follows:

·        5:1         isopropanol to mineral oil             50°C

·        2:1         isopropanol to mineral oil             50°C

·        Undiluted mineral oil                                     50°C

·        Drying oven 5 minutes                                   60°C

·        Coverslip



Do you feel these clearing steps be applied to the pap stain in order to eliminate xylene?  If so, can it be done at room temperature?

Thanks,
Mike

Mike Toole, BS, CT(ASCP)CM