[Histonet] shrinking mouse eyes

[Elizabeth Chlipala]

Nancy

I cannot comment on the plastic embedding but this also happens with paraffin embedded samples.  We trim off one of the edges on all mouse and rat eye samples and that eliminates that problem with paraffin embedded samples.  I can send pictures of a rat eye that has been trimmed.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
liz at premierlab.com
www.premierlab.com

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Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504

-----Original Message-----
From: Thomas, Nancy via Histonet [mailto:histonet at lists.utsouthwestern.edu] 
Sent: Wednesday, May 18, 2016 1:30 PM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] shrinking mouse eyes

Dear experts,
We often get a request to embed and section mouse eyes in GMA.  Currently we have been working with samples from 1 month old mice.  The big problem we need to solve is that the shape of the eyes is fine up until we embed them.  I'm sure the heat from the exothermic reaction is causing the eyes to sink inward, losing their round shape, but  what can prevent this?   We use the Immuno-bed kit which sets up fine and sections well.  Is there something more I can do during dehydration and infiltration steps?  We have experimented with longer dehydration times, but haven't seen much difference.  The eyes were fixed in Davidson's for 24 hr.  Would longer fixation help?
Thank you in advance for all advice,

Nancy Thomas
Senior Lab Manager, Histology Core
Stowers Institute for Medical Research
1000 E. 50th Street
Kansas City, MO  64110

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