[Histonet] Melanin Bleach

[Jay Lundgren]

Cool trick Jeffrey!

On Tue, May 10, 2016 at 12:14 PM, Maxim Peshkov via Histonet <
histonet at lists.utsouthwestern.edu> wrote:

> Karen,
> The bleaching reagents will not compatible before IHC anyway.
> Some tips:
> 1. Do IHC-test as usual and counterstain nuclei with methylene blue
> Loeffler at 3-5 sec, only for
> profile of chromatine. The melanin will stain at green color.
> 2. Use any red color chromogen for IHC. It will be some contrast with
> brown melanine
> 3. Cut sections at 3 microns. Do all steps of IHC as usual, include DAB.
> All next steps do very gently:
> Before nuclear counterstain do bleaching procedure with 0.01% KMnO4 + 0.5%
> H2SO4 at 10 mins;
> gently rinse in DW 1 min;
> 0.5% oxalic acid 5-10 sec or up to bleaching;
> gently rinse in DW 1 min;
> counterstain nuclei and all other steps with regular manner of your lab.
> This solutions will not bleach DAB.
> Sections will not detach from slides.
>
> Hope this help.
> --
>  Maxim Peshkov
>  Russia
>  Taganrog.                         mailto:Maxim_71 at mail.ru
>
>
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