[Histonet] Melanin Bleach

[Rene J Buesa]

You are right. Bleaching is a "rough" procedure for the "survival" of sections and if on top of that you left the section overnight in DiH2O that is a recipe for disaster, as the one you experienced. Try to do the whole procedure during the same day.Additionally it seems to me that 6h in potassium permanganate is too much, you should check the condition of the sections every hour trying to minimize KMnO4 action the least time possible.René 

    On Tuesday, May 10, 2016 12:10 PM, "Heckford, Karen - SMMC-SF via Histonet" <histonet at lists.utsouthwestern.edu> wrote:
 

 Good Morning,
I need some help.  Yesterday I bleached some heavily pigmented tissue.  I have to run some IHC's on them.  I bought the bleaching kit from American Master Tech.  I had to put the Permanganate for about 6 hours to get the melanin and then a couple of minutes in Oxalic Acid.  I had to let them set overnight because I could not get another IHC run in that day.  It looks like the tissue fell off during decloaking.    I used Apex slides.  I rarely ever have to bleach anything here.  So I am not sure if I did this correctly.  I am thinking I need to bleach and do the run in the same day and not let them set over night in DiH20.

Thanks,


Karen Heckford HT ASCP CE
Lead Histology Technician
St. Mary's Medical Center
450 Stanyan St.
San Francisco, Ca. 94117
415-668-1000 ext. 6167
karen.heckford at dignityhealth.org
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