[Histonet] Histonet Digest, Vol 150, Issue 10
Marsha Price
mprice63 at live.com
Sat May 7 15:48:46 CDT 2016
I know this subject has been discussed on here before, but can someone direct me to the correct link for the application to get your Florida HT application? Thank You.
Marsha Price
Sent from my iPhone
> On May 7, 2016, at 12:00 PM, <histonet-request at lists.utsouthwestern.edu> <histonet-request at lists.utsouthwestern.edu> wrote:
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> Today's Topics:
>
> 1. Looking for Part Time Position in Oklahoma
> (Adesupo, Adesuyi (Banjo))
> 2. Re: Automated IHC instrument (Eddie Martin)
> 3. Leica printer (Ginny Achstetter)
> 4. Histology supervisor position - Children's Minnesota
> (Megan Dishop)
> 5. Re: picric acid (E. Wayne Johnson)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Fri, 6 May 2016 12:08:19 -0500
> From: "Adesupo, Adesuyi (Banjo)" <abadesuyi at nrh-ok.com>
> To: "'histonet at lists.utsouthwestern.edu'"
> <histonet at lists.utsouthwestern.edu>
> Subject: [Histonet] Looking for Part Time Position in Oklahoma
> Message-ID:
> <04EE4F75BB5FB246ADB68D69B7460443A5177B10DC at MAIL.nrhnt.nrh-ok.com>
> Content-Type: text/plain; charset="us-ascii"
>
>
> Hi,
> I am looking for a part time histo position in Oklahoma City, OK.
>
> Thanks,
>
>
> Banjo Adesuyi, BMLS, HT (ASCP) HTL, QIHC, QLS
>
> Histology Supervisor
>
> Norman Regional Health System,
>
> Norman, OK 73071.
>
> Cell: 405-973-6363
>
> Tel: 405- 307- 1145
> abadesuyi at nrh-ok.com<mailto:abadesuyi at nrh-ok.com>
>
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> ------------------------------
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> Message: 2
> Date: Fri, 6 May 2016 13:58:47 -0500
> From: Eddie Martin <edmartin26 at gmail.com>
> To: histonet at lists.utsouthwestern.edu
> Subject: Re: [Histonet] Automated IHC instrument
> Message-ID: <069D742F-813A-4A3B-8468-5DDD6B451D1D at gmail.com>
> Content-Type: text/plain; charset=utf-8
>
> Hi Valerie,
>
> Any platform would get the job done well. They all offer reagent rental agreements too. Dako and Leica would require the user to be a bit more knowledgeable of IHC protocols than starting a run on a Ventana analyzer. Dako is semi-automated, whereas leica and ventana both have onboard retrieval, and reagent mixing of chromagen. In terms of the most open system, Dako would be the best analyzer followed by Leica in second place. Ventana does allow 3rd party antibodies to be run on it, but charge you a premium for open containers to run on their analyzer.
>
> In terms of antibodies: this may be better chosen by your pathologist which may have an impact on which analyzer you will also get, but I can suggest to you my favorites.
>
> Her2neu - all three vendors provide a good Her2neu clone. Dako?s Her2 sometimes doesn?t stain as well, so would be my least favorite of the 3. Ventana?s Her2 is the most widely used.
>
> ER - all 3 vendors are also good.
>
> PR - Dako and Novocastra have great PR clones. Ventana?s PR clone is IVD as Dako and Novocastra Clone but is not ASCO-CAP approved antibody. Ventana will push to say it is IVD, as Dako, and Novocastra (Leica), which is necessary for billing purposes, but their PR clone isn?t as effective as the other two i mentioned.
>
> Ki67 - Dako?s MIB-1 clone is like the gold standard for Ki-67. All three vendor versions work as intended. Leica removed one of their two clone options last year, so k2 clone for Leica is your only option. Ventana?s clone looks nicer in my opinion.
>
> Hope this helps somehow.
>
> Kind Regards,
>
> Eddie Martin, HT(ASCP), QIHC, HTL
> 954-826-9403
> Edmartin26 at gmail.com
>
>
> ------------------------------
>
> Message: 3
> Date: Fri, 6 May 2016 18:57:15 -0400
> From: Ginny Achstetter <gachstetter at yahoo.com>
> To: histonet at lists.utsouthwestern.edu
> Subject: [Histonet] Leica printer
> Message-ID: <90D0CF47-7BDF-4228-B3A9-42671784CD26 at yahoo.com>
> Content-Type: text/plain; charset=us-ascii
>
> The Leica printer is a high output machine. Seems like the slide mate is more of a personal printer to go next to every microtome. I also found out that the Leica slides are charged. They have an x on the bottom of the slide. Fisher also makes the rounded edge slides that can be used on that machine.
>
> Sent from my iPhone
>
>
>
> ------------------------------
>
> Message: 4
> Date: Fri, 6 May 2016 17:59:06 -0500
> From: "Megan Dishop" <Megan.Dishop at childrensmn.org>
> To: histonet at lists.utsouthwestern.edu
> Cc: "Jennifer Heimkes" <Jennifer.Heimkes at childrensmn.org>, "Melissa
> Turner" <Melissa.Turner at childrensmn.org>
> Subject: [Histonet] Histology supervisor position - Children's
> Minnesota
> Message-ID: <572CDB6A.D202.00E3.1 at childrensmn.org>
> Content-Type: text/plain; charset="utf-8"
>
> We are looking for a team lead for the histology and immunohistochemistry laboratory at Children's Hospitals and Clinics of Minnesota in Minneapolis-St. Paul. This is a great opportunity in a growing pediatric healthcare network!
>
> Please apply using the link below.
> http://childrensmn.taleo.net/careersection/ex/jobdetail.ftl?job=1602705I
>
>
>
> Megan K. Dishop MD
> Medical Director, Pediatric Anatomic Pathology
> Children's Hospitals and Clinics of Minnesota Laboratories
> 2525 Chicago Ave S. MS32-B600, Minneapolis, MN 55404 USA
> Phone: 612-813-6521 Fax: 612-813-7721 Email: megan.dishop at childrensMN.org
> Adjoint Professor of Pediatrics, University of Colorado School of Medicine
>
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> Message: 5
> Date: Sat, 7 May 2016 10:13:10 +0800
> From: "E. Wayne Johnson" <ewj at pigsqq.org>
> To: histonet at lists.utsouthwestern.edu
> Subject: Re: [Histonet] picric acid
> Message-ID: <67682c47-08a5-435d-5a1c-2b40c2c07e42 at pigsqq.org>
> Content-Type: text/plain; charset=windows-1252; format=flowed
>
> I read "Chemical Magic" and the "Anarchist's Handbook" in high school
> many years ago. That was back when boys carried pocket knives and
> sometime took their shotguns to school to show their friends. I still
> occasionally make a little bit of NI_3 for fun.
>
> We've tried to make picric acid explode and have taken it out in the
> yard and burned it and beat on it with hammers. It's really not all that
> easy to make it go "BOOM" or even make crisp pops. Just don't mix it
> with heavy metals.
>
> I agree strongly that it should be handled with respect, and with gloved
> hands like any staining powder. Store it wet, but the /~~paranoia~/~
> about picric acid is exactly that.
>
> And on the part of the cops and bomb squads who take picric acid out and
> detonate it amid much drama and fanfare, heavy on the delusions and
> illusions of grandeur.
>
> E. Wayne Johnson, DVM
>
> Enable Ag-Tech
>
> Beijing
>
>> On 05/07/2016 12:01 AM, Julio Benavides wrote:
>> Thank you so much everybody for your help!!
>>
>>
>>
>> "Morken, Timothy" <Timothy.Morken at ucsf.edu> escribi?:
>>
>>> Here's another good document on how to handle picric acid powder
>>>
>>> www.ehs.wisc.edu/chem/SafeHandlingOfPicricAcid.pdf
>>>
>>>
>>>
>>> -----Original Message-----
>>> From: Morken, Timothy via Histonet
>>> [mailto:histonet at lists.utsouthwestern.edu]
>>> Sent: Friday, May 06, 2016 8:28 AM
>>> To: Julio Benavides
>>> Cc: Histonet
>>> Subject: Re: [Histonet] picric acid
>>>
>>> Julio, you can just pour water into the container. We always
>>> oversaturated so that a layer of water was on top of the powder.
>>>
>>> Look at this explanation
>>> http://oag.ca.gov/sites/all/files/agweb/pdfs/cci/safety/picric.pdf
>>>
>>> or read the text below if you cannot open this. This contains
>>> instructions on how to properly store picric acid powder, and how to
>>> deal with dry powder found in the lab.
>>>
>>> Long ago I had the pleasure of discovering a batch of six 2kg bottles
>>> of dry picric acid in our "bunker" where we stored flammables. Over
>>> 10 years old according to dates on the box. We called in the fire
>>> department to take care of it. They hosed it down, removed it and
>>> disposed of it; how, I don't know.
>>>
>>>
>>> Tim Morken
>>> Pathology Site Manager, Parnassus
>>> Supervisor, Electron Microscopy/Neuromuscular Special Studies
>>> Department of Pathology UC San Francisco Medical Center
>>>
>>>
>>> +++++++++++++++
>>> PICRIC ACID HAZARDS
>>> Mark Cameron, CIH
>>> Every couple months, an article appears in the local paper about a
>>> bomb disposal team removing picric acid that was found in a
>>> laboratory. The material is usually taken to be blown up. So why is
>>> picric acid considered so dangerous? Well, let?s look at the history
>>> of the use of Picric Acid and see what can be done to avoid those
>>> types of situations.
>>>
>>> Picric Acid (2,4,6 Trinitrophenol) is frequently found in forensic
>>> laboratories for use in the Christmas Tree stain (1) and for Urine
>>> detection (2). Histology uses include connective tissue stain
>>> (Jullien?s picroindogocarmine and Van Gieson?s picro-acid fuchsin),
>>> cytoplasmic stain (Van Gieson?s with iron hematoxylin), woody
>>> sections (picro aniline blue) and as a fixative agent (3). It was
>>> used in medicinal formulations in the treatment of malaria,
>>> trichinosis, herpes, smallpox and antiseptics. A one- percent
>>> solution was also used in the treatment of burns (4).
>>> British Chemist Peter Woulfe discovered picric acid in 1771. Picric
>>> acid was named from the Greek word pikros, which means ?bitter? due
>>> to its bitter taste (5). It was used to dye silk and wool yellow.
>>> Workers making picric acid during World War I were called ?canaries?
>>> because their skin was stained yellow (6).
>>>
>>> The explosive characteristics of Picric acid were discovered early.
>>> In 1885, experiments with picric acid were conducted in Lydd, England
>>> and the English adopted it as an explosive material called Lyddite in
>>> 1888. It was used extensively in bombs and grenades during World War
>>> I (7). Anhydrous Picric acid is similar to TNT. It needs usually
>>> needs a ?booster? such as a primer to create the explosion. However,
>>> as a strong acid, picric acid attacks common metals (except tin and
>>> aluminum) creating explosive salts, which are shock-sensitive. Bombs,
>>> mines and grenades were coated with tin or ashpatim to prevent the
>>> picric acid from contacting the metallic shell (8).
>>>
>>> Several catastrophic events involving picric acid have occurred. On
>>> December 6, 1917, an ammunition ship in Nova Scotia carrying 2,300
>>> tons of picric acid as well as 400,000 pounds of TNT caught fire and
>>> exploded. Over 1,900 people were killed immediately and 9,000 were
>>> injured (9). Shock-sensitive metal picrates demonstrated their
>>> hazardous nature on May 1, 1916 when a fire at a French ammunition
>>> factory caused molten picric acid to flow onto the concrete floor.
>>> Calcium picrate was formed and detonated, killing 170 people (10).
>>> 06/18/02
>>>
>>> Have there been any explosions in laboratories? There are no
>>> documented instances of spontaneous detonation of picric acid in a
>>> laboratory (11). The Department of Transportation classifies Picric
>>> Acid (Trinitrophenol) with less than 30% water by mass as a Class
>>> 1.1D explosive; with greater than 10% water by volume, it is a class
>>> 4.1 flammable solid (12). In the wetted state, it is unlikely to be
>>> an explosive hazard. If a bomb squad tries to blow it up, the picric
>>> acid will not detonate (13) and will just spread picric all over the
>>> area!
>>> The big concern has been with finding dehydrated picric acid. The
>>> most dangerous situations is if the bottle is old and has a metal
>>> cap. Under these circumstances, shock sensitive metal picrates may
>>> have formed on the cap contact area. Explosive experts should be
>>> contacted under these situations. Knowledgeable bomb disposal experts
>>> will use a robot to pick up the container and place it in water to
>>> re-hydrate the material (14) or remove it for detonation elsewhere.
>>>
>>> If a plastic cap is present, and the acid inside has dried, some
>>> crystals may be on the threads and the friction of removing a plastic
>>> cap might be enough to detonate the container. Under these
>>> circumstances, the container may be safe enough to place in a pail of
>>> water. Submerge the bottle to allow water to enter the cap and
>>> threads and dissolve any crystals that might be on the threads. Add
>>> ice to cause shrinkage of the bottle to enhance penetration of the
>>> water. Leave it like this for several days, until water can be seen
>>> inside the bottle. At this point, it is safe to open the cap and
>>> re-hydrate the acid inside (15). Whenever in doubt, contact
>>> explosives experts.
>>> Of course, an ounce of prevention is worth a pound of cure. If you
>>> really need to have picric acid in your lab, here?s what you should do:
>>> 1.
>>> Make sure that the picric acid is kept wet! Do not open a new bottle
>>> until needed. Then date the container to show when it was first used
>>> to help you in a routine inspection program. As part of your lab
>>> inspection program, check the hydration of your picric acid at least
>>> every six months and add distilled water as necessary.
>>> 2.
>>> Do not use metal spatulas to remove the material.
>>> 3.
>>> Be sure to clean the bottleneck, cap and threads with a wet cloth
>>> before resealing (16).
>>> 4.
>>> Get rid of old bottles with metal caps
>>> 5.
>>> Do not store large amounts of picric acid. Dispose of your picric
>>> acid every two years (17).
>>> 6.
>>> If possible, eliminate it from your inventory by purchasing premixed
>>> stains or a 1% solution for using in stain preparation.
>>> If you decide to dispose of your wet picric acid, several options are
>>> available. First, you could try reducing the picric acid to a
>>> non-explosive form using sodium hydroxide and sodium sulfide (18).
>>> After this treatment, the material will still be toxic and have to be
>>> disposed of as hazardous waste. Alternatively, it could be manifested
>>> as a flammable solid for hazardous waste and disposed of by
>>> incineration. DO NOT pour it down the drain; it could react with
>>> copper or iron piping to form the explosive salts.
>>> As a last consideration, Picric Acid is toxic. Ingestion of 1-2 grams
>>> would cause severe poisoning. The dust is irritating to the skin and
>>> eye. A peculiar effect on the eye is ?yellow? tainted vision.
>>> Systemic poisoning causes headache, vertigo, nausea, vomiting and
>>> diarrhea. The skin will turn yellow in severe exposures. Red colored
>>> urine may be produced (19). These symptoms would not expected in the
>>> laboratory environment under traditional uses.
>>>
>>> REFERENCES
>>> 1.
>>> Gaensslen, R., Mertens, J., Lee, H., Stolotow, M., ?Staining and
>>> Extraction Techniques?, Proceeding of a Forensic Science Symposium on
>>> the Analysis of Sexual Assault Evidence., FBI Academy, 1983.
>>> 2.
>>> Slot C. ?Plasma creatinine determination. A new and specific Jaffe
>>> reaction method.? Scand J. Clin. Lab. Invest. 1965, 17: 381
>>> 3.
>>> Lillie, R.D., ?H.J. Conn?s Biological Stains?, Williams & Wilkins
>>> Company, 1969, Baltimore, MD, pages 5, 60-61.
>>> 4.
>>> Patty?s Toxicology, John Wiley & Sons: New York, 2000, Volume IIB,
>>> page 980.
>>> 5.
>>> Davis, Tenney, ?The Chemistry of Powder and Explosives?, Angriff
>>> Press, 1984, page 164.
>>> 6.
>>> Hamilton, Alice, ?Exploring the Dangerous Trades?, American
>>> Industrial Hygiene Association: Fairfax, Virginia, 1995, page 185.
>>> 7.
>>> Cooper, Paul, ?Explosives Engineering?, Wiley-VCH, 1996, page 33.
>>> 8.
>>> Davis, ibid.
>>> 9.
>>> Phifer, Russell, ?Picric Acid: When is Panic Justified??, Speaking of
>>> Safety, Volume 9, No. 2, 2000, page 1-3.
>>> 10.
>>> Medard, Louis, ?Accidental Explosions, Volume 2: Types of Explosive
>>> Substances?, John Wiley and Sons: New York, 1989, page 739.
>>> 11.
>>> Phifer, ibid.
>>> 12.
>>> Code of Federal Regulations, Title 49, Section 172.101.
>>> 13.
>>> Kraut, Irv, In Handbook of Chemical Health and Safety, Alaimo, Robert
>>> J., Ed., Oxford University Press; New York, 2001, page 406.
>>> 14.
>>> Personal Communication with Tom Gundlach of RHR Inc., August 23, 2000.
>>> 15.
>>> Guidance for the Management of Reactive Chemicals, Picric Acid,
>>> http://www.uwsa.edu/oslp/ehs/info/picric.htm , 8/97 revision.
>>> 16.
>>> Safe Use and Management of Picric Acid, Safety Net #104,
>>> http://wwwehs.ucdavis.edu/sflynet/sn-104.html ,11/21/01
>>> 17.
>>> Biological & Chemical Safety Code, Appendix H-3, Handling Procedures
>>> for Unstable Agents, University of Saskatchewan, Department of Health
>>> and Safety, http://duke.usask.ca/~whiterv/unstable.html, 11/21/01.
>>> 18.
>>> Lunn, George and Sansone, Eric B., ?Destruction of Hazardous
>>> Chemicals in the Laboratory?, John Wiley & Sons: New York, 1990, page
>>> 219-221.
>>> 06/18/02
>>> 19.
>>> Documentation of the Threshold Limit Values, American Conference of
>>> Governmental Industrial Hygienists, Inc.: Cincinnati, Ohio, 1991,
>>> page 1271.
>>> 06/18/02
>>>
>>>
>>> +++++++++++++++++++++++++++++++++++++++
>>> -----Original Message-----
>>> From: Julio Benavides via Histonet
>>> [mailto:histonet at lists.utsouthwestern.edu]
>>> Sent: Friday, May 06, 2016 7:11 AM
>>> To: histonet at lists.utsouthwestern.edu
>>> Subject: Re: [Histonet] picric acid
>>>
>>> Hi,
>>>
>>> For how long can you keep it in water? any particular dilution or
>>> just keep it humid (saturation)?
>>>
>>> We also do have some dry picric acid in the lab and, after reading
>>> about the bomb squad, I was begining to get concerned...
>>>
>>> Thanks a lot
>>>
>>> julio
>>>
>>>
>>> El 06/05/2016 a las 15:30, Rene J Buesa via Histonet escribi?:
>>>> Picric acid is an expensive reagent useful in many histology
>>>> procedures.The advise you received of adding water is a good one.Humid
>>>> picric acid will not explode at all. Why waste a good reagent?Keep
>>>> humid, you will eventually used it.Ren?
>>>>
>>>> On Thursday, May 5, 2016 3:24 PM, Mca Werdler via Histonet
>>>> <histonet at lists.utsouthwestern.edu> wrote:
>>>>
>>>>
>>>> Dear histonetters,
>>>>
>>>> Since a few months, i started working in a histology lab, run only by
>>>> me ( coworkers are not specialized in histology). There has not worked
>>>> here a person at histology for about 2 years.
>>>>
>>>> After many new protocols, i decided to clear out some chemicals.
>>>> Now i found around 1 KG of DRY picric acid. I informed my coworkers
>>>> about this, and they said just to dissolve everything in water.
>>>>
>>>> What do you guys think is the best way for handeling with this
>>>> explosive chemical? Thank you all in advance!
>>>>
>>>> Maarten
>>>> _______________________________________________
>>>> Histonet mailing list
>>>> Histonet at lists.utsouthwestern.edu
>>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>>>
>>>>
>>>>
>>>> _______________________________________________
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>>>
>>>
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>>
>>
>>
>>
>>
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