[Histonet] PAS Stain
Jennifer MacDonald
JMacDonald at mtsac.edu
Thu May 5 13:15:00 CDT 2016
We switched form malt diastase to alpha amylase and have seen a
significant improvement in digestion, but as others have said enzyme
activity will be destroyed at 60 degrees C. We put ours into a 37 degree C
water bath. Enzymes work optimally at 37C, body temperature. As the
temperature rises above 37C you will see a decrease in enzyme activity and
then none at all.
Jennifer
From: Joanne Clark via Histonet <histonet at lists.utsouthwestern.edu>
To: "histonet at lists.utsouthwestern.edu"
<histonet at lists.utsouthwestern.edu>
Date: 05/04/2016 01:04 PM
Subject: [Histonet] PAS Stain
Hi Histonetters, I'm hoping someone can help me troubleshoot our PAS
diastase method. We have been digesting the tissue in 0.5% diastase of
malt in a 60 degree oven for 30 minutes, but do not see the glycogen being
digested out. I have tried alpha amylase and beta amylase also without
any luck. Does anyone have any suggestions to get the digestion to work
Joanne Clark, BAAS, HT(ASCP)CM
Director of Histology
P. (575) 622-5600
C. (575) 317-6403
F. (575) 622-3720
TF. (800) 753-7284
pcnm.com
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