[Histonet] PAS Stain

[Jennifer MacDonald]

We switched form malt diastase to alpha amylase and have seen a 
significant improvement in digestion, but as others have said enzyme 
activity will be destroyed at 60 degrees C. We put ours into a 37 degree C 
water bath.  Enzymes work optimally at 37C, body temperature.  As the 
temperature rises above 37C you will see a decrease in enzyme activity and 
then none at all.
Jennifer



From:   Joanne Clark via Histonet <histonet at lists.utsouthwestern.edu>
To:     "histonet at lists.utsouthwestern.edu" 
<histonet at lists.utsouthwestern.edu>
Date:   05/04/2016 01:04 PM
Subject:        [Histonet] PAS Stain



Hi Histonetters, I'm hoping someone can help me troubleshoot our PAS 
diastase method.  We have been digesting the tissue in 0.5% diastase of 
malt in a 60 degree oven for 30 minutes, but do not see the glycogen being 
digested out.  I have tried alpha amylase and beta amylase also without 
any luck.  Does anyone have any suggestions to get the digestion to work

Joanne Clark, BAAS, HT(ASCP)CM
Director of Histology

P.   (575) 622-5600
C.   (575) 317-6403
F.   (575) 622-3720
TF. (800) 753-7284

pcnm.com




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