[Histonet] PAS Stain

[Bob Richmond]

Amylase (diastase) for the PAS stain queries:

Whatever happened to spitting on the slide (30 min at room temperature)?
John Kiernan advises "thinking of lemons and drooling into a small beaker"
though I'd advise chewing on a rubber band for a few seconds.

He notes that alpha amylase is preferred. I'd go with the cheapest one in
the Sigma-Aldrich catalog. Room temperature is usual, but I note that Sigma
offers a heat-stable alpha amylase.

Bob Richmond
Samurai Pathologist
Maryville TN