[Histonet] Histonet Digest, Vol 150, Issue 2
T H
thigginsht at msn.com
Tue May 3 15:03:40 CDT 2016
Good to know that I am not the only on that think Rene is negative to others. Hey Rene it's not just one person that thinks your a dirt bag.
________________________________________
From: histonet-request at lists.utsouthwestern.edu <histonet-request at lists.utsouthwestern.edu>
Sent: Tuesday, May 3, 2016 12:00 PM
To: histonet at lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 150, Issue 2
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Today's Topics:
1. No More Blog Posts -- Over and Out! (Lester Raff MD)
2. IHC positive controls (Cindy Bulmer)
3. Re: No More Blog Posts -- Over and Out! (Rene J Buesa)
4. Fwd: (Linda Margraf)
5. Re: No More Blog Posts -- Over and Out! (Manfre, Philip)
6. Re: IHC positive controls (Morken, Timothy)
7. Re: No More Blog Posts -- Over and Out! (Boyd, Debbie M)
8. Re: No More Blog Posts -- Over and Out! (WILLIAM DESALVO)
9. Re: No More Blog Posts -- Over and Out! (Caroline Miller)
10. Re: No More Blog Posts -- Over and Out! (WILLIAM DESALVO)
11. Re: Fwd: (Rene J Buesa)
12. HHV-8 RTU (Delray Beach Pathology Kari Simeone)
13. PAS/Decal Question (Marcum, Pamela A)
----------------------------------------------------------------------
Message: 1
Date: Mon, 2 May 2016 17:39:28 +0000
From: Lester Raff MD <LRaff at uropartners.com>
To: "'histonet at lists.utsouthwestern.edu'"
<histonet at lists.utsouthwestern.edu>
Subject: [Histonet] No More Blog Posts -- Over and Out!
Message-ID:
<6347C6D2B080534F9B5C2B08436DCFAF10D5E354 at COLOEXCH01.uropartners.local>
Content-Type: text/plain; charset="us-ascii"
To My Lab Colleagues:
As my intent has never been to sow discontent or rancor, I think it is for the best if I no longer post links to my blog, lab related or otherwise. Of course the blogs go on, and if anyone is interested in being added to my mailing list for future notifications, just drop me a line at les.raff at post.com<mailto:les.raff at post.com> The mailing list is never used for any purpose other than announcing a new blog post. Be sure to let me know you are from the Histonet list!
I will continue to participate in any histology/pathologist discussions here as I have for many years.
Cheers,
Lester J. Raff, MD MBA
UroPartners
Medical Director Of Laboratory
2225 Enterprise Dr. Suite 2511
Westchester, Il 60154
Tel: 708-486-0076
Fax: 708-492-0203
------------------------------
Message: 2
Date: Mon, 2 May 2016 13:42:36 -0400
From: Cindy Bulmer <cjbulmer2526 at aol.com>
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] IHC positive controls
Message-ID: <15472908bba-2169-bd52 at webprd-a99.mail.aol.com>
Content-Type: text/plain; charset=utf-8
Hello Histoland,
I need some advice, I have a PT block that is positive with Spirochetes.
What would be the best way to use this block as a positive control?
1) Cut (serial sections, stain the last slide for bugs) and oven time (60) for 1 hr.
then put slides in refrigerator for future use.
2) Cut (serial sections, stain the last slide for bugs) NO oven time and put slides
directly in refrigerator for future use.
3) Cut "fresh" every time they order the Ab.
Thank you,
Cindy
Cynthia Bulmer HT(ASCP),QIHC
IHC Supervisor, CTPL
Waco, TX
------------------------------
Message: 3
Date: Mon, 2 May 2016 18:22:59 +0000 (UTC)
From: Rene J Buesa <rjbuesa at yahoo.com>
To: Lester Raff MD <LRaff at uropartners.com>,
"'histonet at lists.utsouthwestern.edu'"
<histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] No More Blog Posts -- Over and Out!
Message-ID:
<1946825210.5059374.1462213379630.JavaMail.yahoo at mail.yahoo.com>
Content-Type: text/plain; charset=UTF-8
Thank you VERY MUCH!!!!!!!!!!!!!!!!!!!!!!!!!!!!!Ren?
On Monday, May 2, 2016 1:54 PM, Lester Raff MD via Histonet <histonet at lists.utsouthwestern.edu> wrote:
To My Lab Colleagues:
As my intent has never been to sow discontent or rancor, I think it is for the best if I no longer post links to my blog, lab related or otherwise.? Of course the blogs go on, and if anyone is interested in being added to my mailing list for future notifications, just drop me a line at les.raff at post.com<mailto:les.raff at post.com>? The mailing list is never used for any purpose other than announcing a new blog post. Be sure to let me know you are from the Histonet list!
I will continue to participate in any histology/pathologist discussions here as I have for many years.
Cheers,
Lester J. Raff, MD MBA
UroPartners
Medical Director Of Laboratory
2225 Enterprise Dr. Suite 2511
Westchester, Il 60154
Tel: 708-486-0076
Fax: 708-492-0203
_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 4
Date: Mon, 2 May 2016 13:37:35 -0500
From: Linda Margraf <lindamargraf at gmail.com>
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Fwd:
Message-ID: <31CF79C7-84C3-45B8-AF7B-20151C22F338 at gmail.com>
Content-Type: text/plain; charset=us-ascii
> From: Cindy Bulmer <cjbulmer2526 at aol.com>
> Date: May 2, 2016 at 12:15:22 PM CDT
> To: histonet-owner at lists.utsouthwestern.edu
>
> Hello Histoland,
>
> I need some advice, I have a PT block that is positive with Spirochetes.
> What would be the best way to use this block as a positive control?
>
> 1) Cut (serial sections, stain the last slide for bugs) and oven time (60) for 1 hr.
> then put slides in refrigerator for future use.
> 2) Cut (serial sections, stain the last slide for bugs) NO oven time and put slides
> directly in refrigerator for future use.
> 3) Cut "fresh" every time they order the Ab.
>
> Thank you,
> Cindy
> Cynthia Bulmer HT(ASCP),QIHC
> IHC Supervisor, CTPL
> Waco, TX
------------------------------
Message: 5
Date: Mon, 2 May 2016 14:38:32 -0400
From: "Manfre, Philip" <philip_manfre at merck.com>
To: Rene J Buesa <rjbuesa at yahoo.com>
Cc: "'histonet at lists.utsouthwestern.edu'"
<histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] No More Blog Posts -- Over and Out!
Message-ID:
<558A4571351D0C42BD923F403F4198C40111D88D1C5C at USCTMXP51014.merck.com>
Content-Type: text/plain; charset="utf-8"
Delete button broken, huh. I suppose it was necessary to attack a colleague to feel puffed up about yourself. Bully for you!
-----Original Message-----
From: Rene J Buesa via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: Monday, May 02, 2016 2:23 PM
To: Lester Raff MD; 'histonet at lists.utsouthwestern.edu'
Subject: Re: [Histonet] No More Blog Posts -- Over and Out!
Thank you VERY MUCH!!!!!!!!!!!!!!!!!!!!!!!!!!!!!Ren?
On Monday, May 2, 2016 1:54 PM, Lester Raff MD via Histonet <histonet at lists.utsouthwestern.edu> wrote:
To My Lab Colleagues:
As my intent has never been to sow discontent or rancor, I think it is for the best if I no longer post links to my blog, lab related or otherwise.? Of course the blogs go on, and if anyone is interested in being added to my mailing list for future notifications, just drop me a line at les.raff at post.com<mailto:les.raff at post.com>? The mailing list is never used for any purpose other than announcing a new blog post. Be sure to let me know you are from the Histonet list!
I will continue to participate in any histology/pathologist discussions here as I have for many years.
Cheers,
Lester J. Raff, MD MBA
UroPartners
Medical Director Of Laboratory
2225 Enterprise Dr. Suite 2511
Westchester, Il 60154
Tel: 708-486-0076
Fax: 708-492-0203
_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Notice: This e-mail message, together with any attachments, contains
information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth,
New Jersey, USA 07033), and/or its affiliates Direct contact information
for affiliates is available at
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from
your system.
------------------------------
Message: 6
Date: Mon, 2 May 2016 18:47:08 +0000
From: "Morken, Timothy" <Timothy.Morken at ucsf.edu>
To: Cindy Bulmer <cjbulmer2526 at aol.com>
Cc: Histonet <histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] IHC positive controls
Message-ID:
<761E2B5697F795489C8710BCC72141FF6FD47684 at ex07.net.ucsf.edu>
Content-Type: text/plain; charset="us-ascii"
Cynthia, best practice is to keep in the block and cut as needed. Second is to cut and dry at room temp and not melt, then store. Don't cut, melt and store. The issue is oxidation. Keeping in paraffin prevents oxidation. You'll protect the antigens better if they are fully isolated from air, which paraffin does, but once cut, they are exposed. That happens in the fridge as well and I'm not sure refrigeration helps much. I'm guessing that you don't do a lot of these, so you don't want to cut a lot of slide and have them sitting around for months waiting for use.
Tim Morken
Pathology Site Manager, Parnassus
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center
-----Original Message-----
From: Cindy Bulmer via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: Monday, May 02, 2016 10:43 AM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] IHC positive controls
Hello Histoland,
I need some advice, I have a PT block that is positive with Spirochetes.
What would be the best way to use this block as a positive control?
1) Cut (serial sections, stain the last slide for bugs) and oven time (60) for 1 hr.
then put slides in refrigerator for future use.
2) Cut (serial sections, stain the last slide for bugs) NO oven time and put slides
directly in refrigerator for future use.
3) Cut "fresh" every time they order the Ab.
Thank you,
Cindy
Cynthia Bulmer HT(ASCP),QIHC
IHC Supervisor, CTPL
Waco, TX
_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 7
Date: Mon, 2 May 2016 19:08:22 +0000
From: "Boyd, Debbie M" <DKBoyd at chs.net>
To: "Manfre, Philip" <philip_manfre at merck.com>
Cc: "'histonet at lists.utsouthwestern.edu'"
<histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] No More Blog Posts -- Over and Out!
Message-ID:
<7EAFE982E328304DA6CE2B677BB76246AB4B7465 at TN001WEXMBX014.US.chs.net>
Content-Type: text/plain; charset="utf-8"
I just feel if you didn't care for the subject/blog (doesn't pertain to you, you don't have any exposure to it etc. ) you could have deleted it without being so mean spirited. I have in the past ignored the mean spirited sparring, but this was a bit much.
Whatever happened to kindness?
Just my two cents....
Debbie M. Boyd HT (ASCP) | Chief Histologist | Southside Regional Medical Center | 200 Medical Park Blvd. | Petersburg, Va. 23805 | PH 804-765-5025 | FAX 804-765-6058
________________________________________
From: Manfre, Philip via Histonet [histonet at lists.utsouthwestern.edu]
Sent: Monday, May 02, 2016 2:38 PM
To: Rene J Buesa
Cc: 'histonet at lists.utsouthwestern.edu'
Subject: [EXTERNAL] Re: [Histonet] No More Blog Posts -- Over and Out!
Delete button broken, huh. I suppose it was necessary to attack a colleague to feel puffed up about yourself. Bully for you!
-----Original Message-----
From: Rene J Buesa via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: Monday, May 02, 2016 2:23 PM
To: Lester Raff MD; 'histonet at lists.utsouthwestern.edu'
Subject: Re: [Histonet] No More Blog Posts -- Over and Out!
Thank you VERY MUCH!!!!!!!!!!!!!!!!!!!!!!!!!!!!!Ren?
On Monday, May 2, 2016 1:54 PM, Lester Raff MD via Histonet <histonet at lists.utsouthwestern.edu> wrote:
To My Lab Colleagues:
As my intent has never been to sow discontent or rancor, I think it is for the best if I no longer post links to my blog, lab related or otherwise. Of course the blogs go on, and if anyone is interested in being added to my mailing list for future notifications, just drop me a line at les.raff at post.com<mailto:les.raff at post.com> The mailing list is never used for any purpose other than announcing a new blog post. Be sure to let me know you are from the Histonet list!
I will continue to participate in any histology/pathologist discussions here as I have for many years.
Cheers,
Lester J. Raff, MD MBA
UroPartners
Medical Director Of Laboratory
2225 Enterprise Dr. Suite 2511
Westchester, Il 60154
Tel: 708-486-0076
Fax: 708-492-0203
_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Notice: This e-mail message, together with any attachments, contains
information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth,
New Jersey, USA 07033), and/or its affiliates Direct contact information
for affiliates is available at
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from
your system.
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--------------------------------------------------------------------------
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------------------------------
Message: 8
Date: Mon, 2 May 2016 12:10:44 -0700
From: WILLIAM DESALVO <wdesalvo.cac at outlook.com>
To: "Manfre, Philip" <philip_manfre at merck.com>, Rene J Buesa
<rjbuesa at yahoo.com>
Cc: "'histonet at lists.utsouthwestern.edu'"
<histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] No More Blog Posts -- Over and Out!
Message-ID: <COL402-EAS17105428354E8AFE011831082790 at phx.gbl>
Content-Type: text/plain; charset="utf-8"
Your response is WAY out of line. Keep it professional.
Sent from my Windows Phone
________________________________
From: Manfre, Philip via Histonet<mailto:histonet at lists.utsouthwestern.edu>
Sent: ?5/?2/?2016 12:08 PM
To: Rene J Buesa<mailto:rjbuesa at yahoo.com>
Cc: 'histonet at lists.utsouthwestern.edu'<mailto:histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] No More Blog Posts -- Over and Out!
Delete button broken, huh. I suppose it was necessary to attack a colleague to feel puffed up about yourself. Bully for you!
-----Original Message-----
From: Rene J Buesa via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: Monday, May 02, 2016 2:23 PM
To: Lester Raff MD; 'histonet at lists.utsouthwestern.edu'
Subject: Re: [Histonet] No More Blog Posts -- Over and Out!
Thank you VERY MUCH!!!!!!!!!!!!!!!!!!!!!!!!!!!!!Ren?
On Monday, May 2, 2016 1:54 PM, Lester Raff MD via Histonet <histonet at lists.utsouthwestern.edu> wrote:
To My Lab Colleagues:
As my intent has never been to sow discontent or rancor, I think it is for the best if I no longer post links to my blog, lab related or otherwise. Of course the blogs go on, and if anyone is interested in being added to my mailing list for future notifications, just drop me a line at les.raff at post.com<mailto:les.raff at post.com>? The mailing list is never used for any purpose other than announcing a new blog post. Be sure to let me know you are from the Histonet list!
I will continue to participate in any histology/pathologist discussions here as I have for many years.
Cheers,
Lester J. Raff, MD MBA
UroPartners
Medical Director Of Laboratory
2225 Enterprise Dr. Suite 2511
Westchester, Il 60154
Tel: 708-486-0076
Fax: 708-492-0203
_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Notice: This e-mail message, together with any attachments, contains
information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth,
New Jersey, USA 07033), and/or its affiliates Direct contact information
for affiliates is available at
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from
your system.
_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 9
Date: Mon, 2 May 2016 14:49:25 -0700
From: Caroline Miller <mills at 3scan.com>
To: Lester Raff MD <LRaff at uropartners.com>
Cc: "histonet at lists.utsouthwestern.edu"
<histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] No More Blog Posts -- Over and Out!
Message-ID:
<CAFTHRQNbrcnN4jyGQypUYEFPrPdyPVjSjJQC=2W4+oRwTtHeKQ at mail.gmail.com>
Content-Type: text/plain; charset=UTF-8
Thank you Lester, I really appreciate your decision, and I look forward to
reading your histology-related histonet posts.
yours,
mills
On Mon, May 2, 2016 at 10:39 AM, Lester Raff MD via Histonet <
histonet at lists.utsouthwestern.edu> wrote:
> To My Lab Colleagues:
>
> As my intent has never been to sow discontent or rancor, I think it is for
> the best if I no longer post links to my blog, lab related or otherwise.
> Of course the blogs go on, and if anyone is interested in being added to my
> mailing list for future notifications, just drop me a line at
> les.raff at post.com<mailto:les.raff at post.com> The mailing list is never
> used for any purpose other than announcing a new blog post. Be sure to let
> me know you are from the Histonet list!
>
> I will continue to participate in any histology/pathologist discussions
> here as I have for many years.
>
> Cheers,
>
> Lester J. Raff, MD MBA
> UroPartners
> Medical Director Of Laboratory
> 2225 Enterprise Dr. Suite 2511
> Westchester, Il 60154
> Tel: 708-486-0076
> Fax: 708-492-0203
>
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
--
Caroline Miller (mills)
Director of Histology
3Scan.com
415 2187297
------------------------------
Message: 10
Date: Mon, 2 May 2016 15:49:09 -0700
From: WILLIAM DESALVO <wdesalvo.cac at outlook.com>
To: STEVEN PINHEIRO <spinheiro at lumc.edu>
Cc: <histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] No More Blog Posts -- Over and Out!
Message-ID: <COL129-W27DE73AED699C88244AA9582790 at phx.gbl>
Content-Type: text/plain; charset="windows-1256"
Another personal e-mail from you that is unwanted. I am feeling like I have my own stalker. You are one of the reasons that individuals will not post or comment, personal and ugly. I cannot imagine why your workplace (LUMC.edu) chose you to represent them. I request that you no longer contact me and make personal attacks. I know nothing of you and you certainly do not know anything about me. If you have something to say, post to the listserve, not me.
> From: SPINHEIRO at lumc.edu
> To: wdesalvo.cac at outlook.com
> Subject: RE: [Histonet] No More Blog Posts -- Over and Out!
> Date: Mon, 2 May 2016 20:15:38 +0000
>
> As is yours. Just can't keep that dictatorial aspect out of your charming consultant two cents worth.
>
> Steve Pinheiro
>
>
> -----Original Message-----
> From: WILLIAM DESALVO via Histonet [mailto:histonet at lists.utsouthwestern.edu]
> Sent: Monday, May 02, 2016 2:11 PM
> To: Manfre, Philip; Rene J Buesa
> Cc: 'histonet at lists.utsouthwestern.edu'
> Subject: Re: [Histonet] No More Blog Posts -- Over and Out!
>
> Your response is WAY out of line. Keep it professional.
>
> Sent from my Windows Phone
> ________________________________
> From: Manfre, Philip via Histonet<mailto:histonet at lists.utsouthwestern.edu>
> Sent: ?5/?2/?2016 12:08 PM
> To: Rene J Buesa<mailto:rjbuesa at yahoo.com>
> Cc: 'histonet at lists.utsouthwestern.edu'<mailto:histonet at lists.utsouthwestern.edu>
> Subject: Re: [Histonet] No More Blog Posts -- Over and Out!
>
> Delete button broken, huh. I suppose it was necessary to attack a colleague to feel puffed up about yourself. Bully for you!
>
> -----Original Message-----
> From: Rene J Buesa via Histonet [mailto:histonet at lists.utsouthwestern.edu]
> Sent: Monday, May 02, 2016 2:23 PM
> To: Lester Raff MD; 'histonet at lists.utsouthwestern.edu'
> Subject: Re: [Histonet] No More Blog Posts -- Over and Out!
>
> Thank you VERY MUCH!!!!!!!!!!!!!!!!!!!!!!!!!!!!!Ren?
>
> On Monday, May 2, 2016 1:54 PM, Lester Raff MD via Histonet <histonet at lists.utsouthwestern.edu> wrote:
>
>
> To My Lab Colleagues:
>
> As my intent has never been to sow discontent or rancor, I think it is for the best if I no longer post links to my blog, lab related or otherwise. Of course the blogs go on, and if anyone is interested in being added to my mailing list for future notifications, just drop me a line at les.raff at post.com<mailto:les.raff at post.com> The mailing list is never used for any purpose other than announcing a new blog post. Be sure to let me know you are from the Histonet list!
>
> I will continue to participate in any histology/pathologist discussions here as I have for many years.
>
> Cheers,
>
> Lester J. Raff, MD MBA
> UroPartners
> Medical Director Of Laboratory
> 2225 Enterprise Dr. Suite 2511
> Westchester, Il 60154
> Tel: 708-486-0076
> Fax: 708-492-0203
>
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at
> http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system.
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> Confidentiality Notice:
> This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email.
------------------------------
Message: 11
Date: Tue, 3 May 2016 13:28:28 +0000 (UTC)
From: Rene J Buesa <rjbuesa at yahoo.com>
To: Linda Margraf <lindamargraf at gmail.com>,
"histonet at lists.utsouthwestern.edu"
<histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Fwd:
Message-ID:
<1663136023.5351238.1462282108474.JavaMail.yahoo at mail.yahoo.com>
Content-Type: text/plain; charset=UTF-8
As I see it, the best solution is "1"Even more: if the piece of tissue is large enough,?cut 1 section and stain ? select at least 2 (+) areas? divide the block into 2 blocks each containing one of those 2 areas?and?by doing so?you would have duplicated the number of possible (+) sections.Ren?
On Monday, May 2, 2016 3:09 PM, Linda Margraf via Histonet <histonet at lists.utsouthwestern.edu> wrote:
> From: Cindy Bulmer <cjbulmer2526 at aol.com>
> Date: May 2, 2016 at 12:15:22 PM CDT
> To: histonet-owner at lists.utsouthwestern.edu
>
> Hello Histoland,
>?
> I need some advice, I have a PT block that is positive with Spirochetes.
> What would be the best way to use this block as a positive control?
>?
> 1)? Cut (serial sections, stain the last slide for bugs) and oven time (60) for 1 hr.
>? ? ? then put slides in refrigerator for future use.
> 2)? Cut (serial sections, stain the last slide for bugs) NO oven time? and put slides
>? ? ? directly in refrigerator for future use.
> 3)? Cut "fresh" every time they order the Ab.
>?
> Thank you,
> Cindy
> Cynthia Bulmer HT(ASCP),QIHC
> IHC Supervisor, CTPL
> Waco, TX
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Message: 12
Date: Tue, 3 May 2016 16:29:18 +0000
From: Delray Beach Pathology Kari Simeone <KSimeone at leavittmgt.com>
To: "histonet at lists.utsouthwestern.edu"
<histonet at lists.utsouthwestern.edu>
Subject: [Histonet] HHV-8 RTU
Message-ID:
<43944B1DBAAC2846B7B9D626B5F1233CC918550A at vm-email.leavittmgt.com>
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My vendor (Cell Marque/Sigma Aldrich) has a YEAR backorder on this IHC RTU. Does anyone have a vendor they purchase from that I can research? Thanks so much.
Kari M Simeone
Histology/Immunohistochemistry Specialist Supervisor
Alternate Laboratory Supervisor
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Message: 13
Date: Tue, 3 May 2016 16:39:16 +0000
From: "Marcum, Pamela A" <PAMarcum at uams.edu>
To: "histonet at lists.utsouthwestern.edu"
<histonet at lists.utsouthwestern.edu>
Subject: [Histonet] PAS/Decal Question
Message-ID: <826681eae65c40b28de6f620b3207f05 at MAIL13M2N1.ad.uams.edu>
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We are still having issues with our PAS stain on decaled bone marrows. The Pathologists in HemePath are seeing what they refer to as smudginess in cells on some areas of the completed PAS slides. We have looked at everything and cannot find where the issue is coming from at this point. We have done manual staining for PAS, automated on the Leica stainer and on the Dako Artisan. All methods show the same result for some slides. We can go for several days to a week or more with no problem and then suddenly it is back and we have changed nothing in the way we do the processing, embedding, sectioning, deparaffinization and coverslipping. We do as many as 38 bone marrow cores a night or as few as 8 and can find no correlation in the number we have to deal with for a given period. All bone marrows drawn today must be completed by 8AM tomorrow morning.
Fixation after pulling the bone marrows is a minimum of 2 hours in AZF with a maximum of 7 hours +/-.
Grossed and placed in cassettes for 15 minute rinse in running DI Water
Decal currently in the Milestone Decal Unit for 45 minutes in Immunocal at 50C
Rinsed in running DI water for 15 minutes
Placed in 10% NBF and processed on a 4 hour program with a delay of 4 hours minimum to come off at 4:45AM.
If anyone knows of any literature on decal effects on PAS staining in bone marrows please contact me. This has been going on for months and no matter what we do manual staining, Leica adaptation for automated or Dako it is not helping. Dako has been great with sending in technical experts repeatedly and we cannot get this corrected.
Thanks,
Pam
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End of Histonet Digest, Vol 150, Issue 2
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