[Histonet] Fwd:
Rene J Buesa
rjbuesa at yahoo.com
Tue May 3 08:28:28 CDT 2016
As I see it, the best solution is "1"Even more: if the piece of tissue is large enough,→cut 1 section and stain → select at least 2 (+) areas→ divide the block into 2 blocks each containing one of those 2 areas and by doing so you would have duplicated the number of possible (+) sections.René
On Monday, May 2, 2016 3:09 PM, Linda Margraf via Histonet <histonet at lists.utsouthwestern.edu> wrote:
> From: Cindy Bulmer <cjbulmer2526 at aol.com>
> Date: May 2, 2016 at 12:15:22 PM CDT
> To: histonet-owner at lists.utsouthwestern.edu
>
> Hello Histoland,
>
> I need some advice, I have a PT block that is positive with Spirochetes.
> What would be the best way to use this block as a positive control?
>
> 1) Cut (serial sections, stain the last slide for bugs) and oven time (60) for 1 hr.
> then put slides in refrigerator for future use.
> 2) Cut (serial sections, stain the last slide for bugs) NO oven time and put slides
> directly in refrigerator for future use.
> 3) Cut "fresh" every time they order the Ab.
>
> Thank you,
> Cindy
> Cynthia Bulmer HT(ASCP),QIHC
> IHC Supervisor, CTPL
> Waco, TX
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