[Histonet] PLA2R assay question

[Martha Ward-Pathology]

I have been asked to look into offering PLA2R on our renal biopsies and in doing some research on the subject I find that some people are staining frozen tissue via an indirect IMF procedure and some are staining ffpe tissues, also using an indirect IMF procedure.    I would prefer to do this via traditional IHC (we have Bond 2 stainers).    Is anyone doing this way?   If so, could you provide some tips on getting it to work successfully?

Thanks in advance for any help.


 
Martha Ward, MT (ASCP) QIHC
Manager
 
Molecular Diagnostics Lab
Medical Center Boulevard  \  Winston-Salem, NC 27157
p 336.716.2109  \  f 336.716.5890  
mward at wakehealth.edu