[Histonet] Porcessing FFPE tissue without alcohol??

[Tony Henwood (SCHN)]

You might find these articles to be useful

Tracy, R. E., & Walia, P. (2002). A method to fix lipids for staining fat embolism in paraffin sections. Histopathology, 41(1), 75-79.

Turello, R., Snyder, D., & Hartman, H. A. (1984). A modification the osmium tetroxide post-fixation technique for the demonstration of extracellular lipid in paraffin-embedded tissue sections. Journal of Histotechnology, 7(2), 75-77.


Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-----Original Message-----
From: Hobbs, Carl via Histonet [mailto:histonet at lists.utsouthwestern.edu] 
Sent: Saturday, 26 March 2016 5:30 AM
To: histonet
Subject: Re: [Histonet] Porcessing FFPE tissue without alcohol??



Fix the tissue in Formalin, wash well in dw, then place very small pieces into Osmium tetroxide solution ( std soln for TEM post-fixation) Processing to Pwax as usual.
Basically, you will see lipids as black ( oxidised osmium) That's the only way to demonstrate solvent- soluble lipids, using Pwax processing.
Sure, there are caveats but, in the main...it will be Ok, imho.
I invite comments as I may be doing exactly this very soon, to count myelinated nerve fibres in a sciatic nerve.



  
 
Carl Hobbs FIBMS
Histology and Imaging Manager
Wolfson CARD
Guys Campus, London Bridge
Kings College London
London
SE1 1UL 
  
020 7848 6813    
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